EDITORIAL Promotions for Life Scientists of the...

Promotions for Life Scientists

ISSUE 15 - November�� arch 2010��

EDITORIAL ISSUE 15 - NOVEMBER 2009

Local price agreements apply.

DK

http://dk.vwr.com/lifescience

WWW.VWR.COMEditorVWR International bvbaResearchpark Haasrode 2020Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International

Layout and typesettingMarketing Services VWR

PrintingStork, Germany

No part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe.

Run80.500 copiesPublication date: November 2009

Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.

Cell biology

�� Whatman Klari-FlexTM

�� FBS-Superior – Biochrom�� BD FalconTM tube�� Corning filter systems�� Wheaton serum bottles�� Spectrum CellMax Duo�� Pall VacuCap©

�� Streptavidin magnetic beads from SoluLinK�� Nunc cryogenic tubes

Genomics

�� 5 PRIME Hotmaster Taq�� Thermo Scientific Hot-Start PCR�� 96 well Electroporation from BTX�� Bertin Precellys�� GelGreenTM and GelRedTM from Biotium�� Brand Microplate and cuvettes �� BDH Prolabo Agarose �� GE Healthcare NanoVue Plus�� VWR Collection PCR combo�� Omega Bio Tek Plasmid mini kit�� VWR Collection GenoSmart�� VWR Collection Taq�� DNA extraction from ZyGEM

Proteomics

�� Applichem plate stabiliser�� Cayman Antibodies�� NAP-BLOCKER from G-Biosciences�� Thermo Biopolymers custom peptides�� Sartorius Vivaspin�� Thermo Scientific MultiskanFC�� CBS Vertical electrophoresis and blotting system

combo

Supporting products

�� ART pipette tips reload system

IN THIS ISSUE

��Cell biology Welcome to the Autumn issue

of the VWRbioMarke shop.

As thoughts turn to the end ofthe year celebrations, here atVWR we’ve worked hard to comeup with some offers that aresure to warm you and get you in the festive mood.

There’s some exceptional offers in this issue on everything from Life science equipment like cyclers and gel documentation. Plus somefabulous reductions on everyday consumables for all disciplines.Seasons greetings from yourVWRbioMarke team!

Easy to use: FBS-Superior from Biochrom

Biochrom offers with FBS Superior A standardised quality serum that evens out the usual variations of biological material. Due to the addition of selected synthetic supplements, a continuous high level of quality is guaranteed.

Manage routine cell culture in the future without any need to test FBS batches, and use the Biochrom FBS that provides constant quality and excellent growth-enhancing characteristics.

�� Once tested, the FBS provides a balanced constant growth profile

�� Save further testing in the future�� The costs for the FBS Superior are the same

compared to usual EU-approved FBS

Pack (ml) Cat. No. Price

100 BCHRS0613P 297,85

500 BCHRS0615P 837,70

Streptavidin magnetic beadsThe highest binding capacityStreptavidin magnetic beads available.

promo code

3

Buy 2 pay for 1!

Streptavidin magnetic beads Qty (mg) Cat. No. Price

NanoLink 10 733-2507P 2220,-

MagnaLink 10 733-1883P 2830,-

�� Lower backgrounds of non-specific binding

�� Lower net costs by using less beads�� Available in two sizes��

NEW BD Falcon™ 50 ml conical tube with Flip-Top cap Ideal for any research applications requiring one-handed opening and closing of the cap. This novel design saves time and effort in applications requiring multiple aliquoting, storage, and pouring from the same tube, while maintaining the same superior quality and performance of our standard screw-cap closure.

�� Hinged cap and thumb grip design enables simple, sterile one-hand manipulation

�� Innovative splash guard eliminates risk of splatter�� Engineered to perform under high stress and a broad range of

temperatures�� Novel click-bead feature ensures a tight seal.

Description Size (mm) Sterile Packaged Qty/Pk Cat. No. Price

BD Falcon 50 ml high clarity polypropylene, conical tubes with Flip-Top cap.

30 mm O.D. and 115 mm length

Yes 22/bag 440 734-1437P 958,76

To order your free sample Email [email protected]

promo code

4

promo code

2

Order a free sample today! Contact [email protected]

NEW: Whatman Klari-Flex™ bottle top filtration system with Drop-Connect technologyDesigned to improve productivity, the Klari-Flex™ system features a stable, low-profile docking station with a permanent vacuum hose connection. So you only have to connect the vacuum once!The weighted docking station and the tip-resistant bottle makes the Klari-FlexTM system easy-to-use, maximises throughput and minimises the risk of spills and contamination.Thanks to Drop-Connect technology, the filtration unit can be quickly and accurately placed into the docking station with just one hand. Removing the unit is just as easy.

What will Klari-Flex™ offer you:�� Minimised risk of spills and

contamination�� Improved productivity�� Fail-safe vacuum connection�� Easier to use for one hand operation

promo code

1

Buy 25 packs and get a

docking station free

(Value: 860€ )

Klari-Flex bottle top PES-S filter volume (ml)

Pore size(μm)

Qty/Pk Cat. No. Price

250 0.2 12 514-1158P 830,-

500 0.2 12 514-1159P 1210,-

1000 0.2 12 514-1160P 1570,-

250 0.45 12 514-1164P 807,-

500 0.45 12 514-1165P 1190,-

1000 0.45 12 514-1166P 1540,-

Please request more information via [email protected]

Place your order today! Visit our website http://dk.vwr.comLocal price agreements apply.

Offers valid from 16th November to 31st March 2010Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders.2

ISSUE 15 - NOVEMBER 2009��Corning® filter systemsThe smart choice for Cell Culture filtration

�� Corning’s new vacuum filter products use a new square filter membrane support system that increases filtration rates�� Improved, higher capacity Polyethersulphone (PES) membranes maximise filter throughput�� Together, the new square filter supports with the improved PES membranes increase filtering performance by up to 44% depending on the filter funnel size

Cell biologypromo

code5

Wheaton serum bottlesSerum bottles are ideal for lyophilisation, as vaccine/injectable drug containers and also for short and long term storage. They are made from moulded glass and have excellent wall strength.

�� Fit most lyophilisation applications

�� Borosilicate glass that conforms to USP Type I requirements

�� Excellent chemical resistance

�� Shrink-wrapped partitioned modules reduce breakage

�� Mouth ID x OD 13 x 20 mm

promo code

7

Buy 3 pay

for 2

Clear serum bottles

Size (ml) Dia x H (mm) Qty/Pk Cat. No. Price

5 23 x 47 288 216-3005P 1410,-

10 25 x 54 288 216-3006P 1980,-

20 32 x 58 288 216-3007P 2420,-

30 37 x 63 288 216-3008P 2970,-

50 43 x 73 288 216-3009P 3720,-

60 41 x 91 144 216-3010P 2080,-

100 52 x 95 144 216-3011P 2880,-

125 54 x 107 144 216-3012P 4020,-

Amber serum bottles

Size (ml) Dia x H (mm) Qty/Pk Cat. No. Price

5 23 x 47 288 216-3013P 2540,-

10 25 x 54 288 216-3014P 2820,-

20 32 x 58 288 216-3015P 3190,-

30 37 x 63 288 216-3016P 3890,-

50 43 x 73 288 216-3017P 4950,-

100 52 x 95 144 216-3018P 3710,-

Description Bottle volume (ml) Neck size (mm)

Pore size ��

Qty/Pk

Cat. No. Price

PES membrane

Vacuum filter systems with prefilter

150 50 0.22 12 513-3357P 692,-

250 50 0.22 12 513-3354P 711,-

500 70 0.22 12 513-3355P 1167,-

1000 90 0.22 12 513-3356P 1701,-

Bottle top vacuum filters with prefilter

150 33 0.22 48 734-1890P 1978,-

150 45 0.22 48 734-1891P 1978,-

500 33 0.22 12 513-3362P 735,-

500 45 0.22 12 525-3409P 735,-

1000 45 0.22 12 734-1896P 1239,-

CA membrane


150 50 0.22 12 513-3358P 692,-

150 50 0.45 12 513-3359P 692,-

250 50 0.22 12 513-3346P 711,-

250 50 0.45 12 513-3347P 711,-

500 70 0.22 12 513-3348P 1167,-

500 70 0.45 12 513-3349P 1168,-

1000 90 0.22 12 734-1846P 1700,-

1000 90 0.45 12 734-1845P 1700,-

1000 with 500 Funnel 70 0.22 12 734-1900P 1700,-

1000 with 500 Funnel 70 0.45 12 734-1901P 1700,-

CA membrane


150 33 0.22 48 513-3361P 1978,-

150 33 0.45 48 513-3344P 1978,-

150 45 0.22 48 513-3360P 1978,-

150 45 0.45 48 513-3345P 1978,-

500 33 0.22 12 734-1848P 735,-

500 33 0.45 12 734-1841P 735,-

500 45 0.22 12 734-1842P 735,-

500 45 0.45 12 734-1843P 735,-

1000 45 0.22 12 734-1810P 1243,-

CN membrane


250 50 0.22 12 734-1860P 711,-

500 70 0.22 12 734-1861P 1167,-

1000 90 0.22 12 734-1821P 1700,-

NY membrane


250 50 0.2 12 513-3350P 749,-

500 70 0.2 12 513-3352P 1167,-

1000 90 0.2 12 734-1844P 1700,-


500 45 0.2 12 734-1811P 735,-

150 tube top vacuum filters

50 0.22 12 734-1831P 586,-

50 0.45 12 734-1830P 586,-

33 0.22 48

Buy 3 and

pay for only 2

Thermo Scientific Nunc Cryogenic TubesWhen it is worth preserving, choose the quality you can trust.

Designed for cryogenic storage at liquid nitrogen temperatures, all Thermo Scientific Nunc® CryoTubes are made of non-cytotoxic and USP and EuPh-compliant plastics, ensuring that these cryogenic vials will not affect your cells. The sterile CryoTubes are also CE-marked, meeting the regulatory requirements of the IVD directive for containers of samples of human origin. Finally, the unique plastic used to make Nunc CryoTubes have a very low cell-binding capacity, to allow maximum cell recovery after thawing.Available in various sizes from 1 to 4.5 ml working volume, with either internally as externally-threaded versions, the Nunc CryoTubes have been the standard in cryogenic preservation for more than 40 years. When you need to preserve your valuable cells, you can trust the Nunc CryoTubes.

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Description of Nunc Cryotubes

Bottom Thread Size (ml) Sterile Qty/Pk Cat. No. Price

Narrow neck Round Ext 1 Yes 500 479-6816P 1292,38

Round 1.8 Yes 500 479-6853P 1223,60

Round Int 1.8 Yes 500 479-6837P 1157,67

Round base, self-standing Conical 1 Yes 500 479-6840P 1255,42

Round 1.8 Yes 450 479-6841P 1094,58

Starfoot Conical 1 Yes 500 479-6842P 1275,33

Round 1.8 Yes 450 479-6843P 1101,09

Round base, barcode label Round 1.8 Yes 2000 479-0598P 13296,93

Starfoot, barcode inkjet Round 1.8 Yes 1800 479-0599P 7236,58

Nalgene System100 Cryobox 10 x 10 No 1 479-3314P 94,-

Buy 4 packs of cryovials or 1 pack

of barcoded cryovials & get 10

Nalgene System100 cryoboxes free

(>100 € value !)

Free

product


Offers valid from 16th November to 31st March 2010Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders. 3

ISSUE 15 - NOVEMBER 2009��CELLMAX® DUO SYSTEM

Cell culturing with Hollow Fibre Bioreactors

A low cost alternative to conventional cell culture methods 1 CellMax® Bioreactor is equivalent to the yield from 100 T-Flasks!

Unlike traditional cell culture methods, T-flasks, roller bottles, and two compartment bioreactors, CellMax® Hollow Fibre Bioreactors provide a three dimensional space for in vitro cell culture. Hollow fibres are tubular ��"�#�� $�� &�''��and around the large surface area provided by the network of hollow fibres. When perfused with culture media, the hollow fibres allow oxygen and nutrients to be supplied to the cells while metabolic waste products are eliminated. The process increases the accumulation of the cell-secreted growth factors required for optimal growth. Due to the system’s efficient delivery of media and removal of waste, the process uses less culture media than other methods. Cultures can be sustained for 6 months or more while maintaining high production yields and titres.CellMax® DUO Pump requires less space than traditional cell culture techniques and can be placed in an incubator. The cool running motor does not affect incubator temperature.

Ordering Information For detailed information about the CellMax® DUO System, please ask your local VWR sales office.

promo code

8Process more per unit and reduce plastics waste…….

VacuCap® bottle-top vacuum filtration devices are designed for fast, easy sterile filtration of 100 ml to 5 l.Use less plastic and increase filtration efficiency.VacuCap filters can be transferred to different receiving vessels, so filtration capacity is not limited by a single filtration unit. Fewer filter units are needed, saving money and reducing waste.The VacuCap filter’s patented small design accepts a wide variety of collection vessel sizes and reduces waste and storage space.Ordering information – supplied sterile in packs of 10

promo code

9

Get a free bioreactor of

your choice by purchasing the CellMax® DUO

System!

Description Cat. No. Price Promo Price

CellMax® DUO System for two HF Bioreactors, 120–240 V Flow rate: 5 – 120 ml/min

392-0211P 28180,- 21975,-

Fibre type MWCO Surface Area (cm2) ECS (ml) Cat. No. Price Promo Price

Cellulose 30 kD 1 700 12 392-0178P 5750,- 4481,84

30 kD 10 000 60 392-0209P 7360,- 5740,24

20 kD 16 000 100 392-0147P 8340,- 6504,96

Polysulphone 10 kD 1 700 12 392-0177P 5750,- 4481,84

10 kD 10 000 60 392-0208P 7360,- 5740,24

30 kD 1 300 12 392-0207P 5750,- 4481,84

30 kD 10 000 60 392-0210P 7360,- 5740,24

pre-coated Polypropylene � �� 100 1.5 392-0157P 6450,- 5023,92

� *�� 423 7 392-0158P 7590,- 5914,48

� $�� 123 2.3 392-0159P 7130,- 5556,32

Polypropylene � �� 100 1.5 392-0155P 5780,- 4501,20

� *�� 423 7 392-0148P 5390,- 4201,12

� $�� 123 2.3 392-0154P 5270,- 4104,32

� $�� 1 500 12 392-0149P 5750,- 4481,84

�� Filter faster with the Supor membrane for high flow rates�� Match filter to sample volume by choosing either 60 or 90 mm device�� Maximise throughput of hard-to-filter solutions with VacuCap PF devices with

a built-in prefilter�� No need to constantly refill upper fluid reservoir�� Draws directly from the mixing reservoir�� Eliminate the possibility of contamination from transfer steps as filters directly

into the desired container�� +�;�<��=<��'�� "�� Patented small design accepts a wide variety of collection vessel sizes and

reduces waste and storage space�� Available with tubing attached to each device (TA version) for maximum

convenience

VacuCap 60 Devices (up to 1 l)


� "��>�?�� 515-0016P 1020,- 896,22

� ��>�?�� 515-0017P 1020,- 896,22

� J*��>�?�� 515-0018P 1020,- 896,22

� ��>�?��Q��'�;��;[;��''=�attached tubing for each filter device)

516-9813P 1770,- 1546,42

VacuCap 60 PF Device (up to 1 l)


� �\� ��>�?�� 515-0019P 955,- 955,-

VacuCap 90 Devices (up to 5 l)


� "��>�]�� 515-0012P 1120,- 974,10

� ��>�]�� 515-0013P 1070,- 941,34

� J*��>�]�� 515-0014P 1070,- 939,40

� ��>�]��Q��'�;��;[;��''=�attached tubing for each filter device),

516-9812P 1860,- 1635,62

VacuCap 90 PF Device (up to 5 l)


� �\� ��>�]�� 515-0015P 1210,- 1058,10

Genomics

E.Z.N.A.™ Plasmid Mini Kit llIsolate up to 70 μg of Plasmid DNA in 30 minutes

The E.Z.N.A.™ Plasmid Mini Kit ll bridges the gap between mini and midi prep plasmid DNA isolation protocols. The binding capacity of the HiBind DNA columns is doubled allowing for the processing of up to 15 ml of culture. The whole procedure is completed in a microcentrifuge or a vacuum manifold allowing for easy and fast processing. Purified Plasmid DNA can be used for transfection, transformation, sequencing, restriction enzyme digest and routine screening.

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10

Description Qty/Pk Cat. No. Price Promo Price

E.Z.N.A.™ Plasmid Mini Kit ll 200 OMEGD6945-02P 1480,- 980,68

Buy Plasmid Mini Kit ll 200 Prep size

(D6945-02) and receive a bonus

Plasmid Mini Kit ll 50 Prep size Free

(D6945-01)

BDH Prolabo Electran® DNA grade agaroseBDH Electran® covers the whole range of agarose, each of which is optimally specified for particular applications.

Description Pack Cat. No. Price Promo price

Standard agarose for routine gel electrophoresis

500 g 438794LP 3380,- 950,-

Take advantage of

our HAPPY BIRTHDAY

offer!

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11

Innovations in nucleic acid isolation

SPECIAL OFFER Save your data, whilst helping save the planet.

With every purchase of VacuCap filter units receive a free 1GB USB Memory Stick,

made from recycled rubber.



ISSUE 15 - NOVEMBER 2009��New option for Hot-Start PCR

Now you can load your hot-start PCR product directly onto an agarose gel

Go directly from thermal cycler to gel analysis without having to add additional reagents with help of our unique ReddyMix PCR buffer, now available with our Hot-Start enzyme Thermo-Start Taq DNA Polymerase. This time saving buffer contains both loading dye and gel loading precipitant, saving your time and sample handling steps.

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Master your PCR with 5 PRIME HotMaster™ TaqThree for two on 5 PRIME HotMaster Taq

Fast HotStart Performance Fast PCR Amplification of a 131 bp fragment of the human TFN gene with standard Taq, HotMaster Taq and conventional Hot Start enzymes.All reactions with 5 sec elongation cycle. Initial denaturation was 2 min at 95°C or 10 min for the chemically modified enzyme respectively.

Innovative Hot-Start / Cold-Stop technology for fast and specific Hot-Start PCR�� Optimal results – highly specific amplification�� Save time and effort – optimal PCR conditions provided�� Excellent for fast PCR protocols – no enzyme activation

Hot-start is a well established method for improving PCR specificity. Conventional hot-start technologies, such as antibody-mediated inhibition or chemical blocking of DNA polymerases, have limitations, such as long initial activation steps that can reduce the performance of the DNA polymerase and compromise specificity. HotMaster Taq DNA Polymerase features a superior hot-start PCR technology – Hot-Start / Cold-Stop®: A temperature-dependent inhibitory ligand completely inhibits Taq polymerase activity at low temperatures. At high temperatures, the inhibitor is released and full Taq activity is immediately restored. Since the process is reversible, the “cold-stop” aspect of the inhibitor has the potential to block enzyme activity in every low-temperature cycle of the PCR, ensuring optimal results.The HotMaster Taq is also available as convenient HotMasterMix.

Genomicspromo

code12

Buy two of the same

product and receive a

third free!

Description Content Cat. No. Price

HotMaster Taq DNA Polymerase 250 U 733-0118P 893,54



5 PRIME HotMasterMix 100 rxn 733-0120P 649,88

5 PRIME HotMasterMix 1000 rxn 733-0121P 4908,74

Description Pack (25 ul rxns)

Cat. No. Price Promo price

1.1X Thermo-Start ReddyMix PCR Master Mix

160 x 25 ADVAAB-1960/AP 686,- 480,20

1.1X Thermo-Start ReddyMix PCR Master Mix

1600 x 25 ADVAAB-1960/BP 5490,- 3843,-

2X Thermo-Start ReddyMix PCR Master Mix

160 x 25 ADVAAB-1961/AP 686,- 480,20

2X Thermo-Start ReddyMix PCR Master Mix

1600 x 25 ADVAAB-1961/BP 5490,-

2X Thermo-Start High Performance ReddyMix PCR Master Mix

160 x 25 ADVAAB-1962/AP 853,- 597,10

2X Thermo-Start High Performance ReddyMix PCR Master Mix

1600 x 25 ADVAAB-1962/BP 6290,- 4403,-

Precellys®24-Dual fast, cooled tissue homogeniser and cell lyser

Precellys®24-Dual is a tissue homogeniser for sample preparation and cell lysis in a single system for DNA, RNA, proteins and drugs extraction. The bead-beating technology grinds and homogenises a large range of biological samples in few seconds. Mouse organs can be prepared whole for example brain (2 g), liver (3 g), lung/bronchus/trachea (1.5 g). The system can be operated in multiple configurations with 0.5 ml, 2 ml, 7 ml, with or without Cryolys cooling option.

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�� Fast - Homogenise samples in few seconds�� Cooling option available – Cryolys allows temperature sensitive molecules extraction (RNA, proteins)�� Efficient - Retain integrity of any biological molecule DNA, RNA, protein, drug etc.�� Flexible - Adapt protocols and consumables from soft to hard tissues (micro-organisms, animal or

vegetal tissues) and from 1 mg to 5000 mg�� Cross contamination free – single-use tubes�� Reproducible – Standard protocols�� High-Quality - Robust, ergonomic and programmable

Sorry, not available in France or Germany

New – accommodates 6 x 7 ml or 12 x 12 ml tubes


Precellys 24 - Dual Sized homogeniser including 3 x 7 ml Grinding kits (432-3759)

432-3757P 117620,- 72500,-

Ceramic grinding kits for 50 preps

2.8 mm beads in 7 ml tube 432-3759P 4940,- 3100,-

1.4 mm beads in 0.5 ml tube 432-3760P 2700,- 1700,-

0.5 mm beads in 0.5 ml tube 432-3761P 2700,- 1700,-

mix of 1.4 mm and 2.8 mm beads in 2 ml tube 432-3762P 2700,- 1700,-

38% off and 3 free kits of 7

ml beads

homogeniser aPrecellys®24

and drugs seconds. Msystem ca

��

��

Sor

ga

30% off list price

NanoVue™ Plus GE Healthcare Life Sciences Drop. Measure. Done. The easy to use spectrophotometer.

NanoVue Plus is the latest addition to GE Healthcare’s range of UV/Visible spectrophotometers giving intelligent performance across all small volume spectrophotometer applications. NanoVue Plus quickly and accurately quantifies nucleic acids and protein samples, using a convenient, drop & read sample plate.

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NanoVue Plus 736-0368P 73300,- 65970,-

NanoVue Plus with SD card 736-0369P 75540,- 67986,-

NanoVue Plus with integrated printer 736-0370P 75990,- 68391,-

NanoVue Plus with Bluetooth 736-0371P 75250,- 67725,-

10% OFF�� Swift, accurate analysis of 0.5 - 5 μl, nucleic acid and protein samples

�� Outstanding sample recovery�� Reliable and reproducible measurements �� Pre-defined methods for nucleic acid quantification,

protein quantification, and protein assays

�� Automatic self-calibration and intuitive software �� Up to 90 user-defined methods can be stored with optional

password protection �� Optional built-in printer or output data via USB to a PC for

printing or storage �� Optional wireless connectivity

e e across ples, using a conv

NEW



ISSUE 15 - NOVEMBER 2009��See the advantage of BTX 96 well Electroporation�� Fast library development �� Simplified method optimisation for high yields�� No need for expensive kits

The BTX 25 or 96 well HTS system is a powerful tool for optimising experimental variables in minutes. Run 48 samples in duplicate and in seconds be ready to analyse the results; for example, five different cell densities, three nucleic acid concentration, eight different buffers and up to 8 varied electrical conditions in just minutes. The BTX HT systems allow you to get more data rapidly, optimise for highest yields and save time without expensive reagent kits.

BTX is the leader in Multiwell technology for electroporation.

�� Other electroporation products available for in vivo and in vitro applications

Use BTX HT Systems for Bacteria, Yeast, Mammalian, Plant and Insect cells

UV-transparent BRANDplates® microplates and cuvettes

The new 96 well UV-Plates from BRAND are manufactured according to the ANSI-/SBS-Standard 1 through 4 – 2004. The microplates made in PS have a UV transparent bottom made from a very thin 25 μm fluorocarbon film, ensuring a high light transmission in the wavelength range between 220 nm and 700 nm. The excellent light transmission (85 % at 260 nm, 90 % at 280 nm) allows the precise quantification of nucleic acids and proteins (260 / 280 nm).The disposable micro UV-cuvettes work from 220 nm and are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Standard light path 10 mm. Excellent chemical resistance. Sample volumes as small as 70 μl are sufficient.

�� Individually wrapped UV-cuvettes and free from DNase, DNA and RNase�� 96-well UV-transparent pureGrade™ microplates, F-bottom, 350 μl per well, slightly hydrophilized, non-treated, non-sterile�� UV-cuvettes micro, UV-transparent Polymer, center height 8.5 or 15 mm, fit into most spectrophotometers without adapter, packed

by mould cavity number to ensure lowest variation of extinction coefficient

Are you still using Ethidium Bromide? Then have a look at our environmentally friendly and ultra-sensitive nucleic acid gel stains alternatives....GelRedTM - superior to EtBr

�� Non-mutagenic and non-toxic�� Easy disposal –passes environmental safety test for disposal directly down the drain or in

regular rubbish�� More sensitive than EtBr �� Stable at room temperature, available in water, microwaveable�� Flexible, can be used for dsDNA, ssDNA or RNA in either precast or post gel staining�� Compatible with most downstream applications�� Easy to use –no change to the optical settings used for EtBr are required

Have you already got rid of the Ethidium Bromide?Then GelGreen™ could be of interest...GelGreen™ – is simply unmatched by SYBR® Safe

�� Non-toxic and non-mutagenic, available in water�� Higher stability than SYBR® Green I at equal sensitivity �� More sensitive than SYBR® Safe

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Still not convinced...?Request your personal sample at [email protected]

Description Qty/Pk (ml) Cat. No. Price Promo Price

GelRed 10000x in water 0.5 730-2958P 1167,- 860,38

10 730-2960P 17623,- 13335,86

GelGreen 10000x in water 0.5 730-2961P 1024,- 813,08

10 730-1343P 17850,- 12195,82

Description Plus free of charge Cat. No. Price

1 pack (50pcs.) UV-transparent 96-well BRANDplates®

2 packs (2 x 100 pcs.) UV-cuvettes micro, centre height 8.5 mm, individually wrapped

612-3668P1 5550,-

1 pack (50pcs.) UV-transparent 96-well BRANDplates®

2 packs (2 x 100 pcs.) UV-cuvettes micro, centre height 15 mm, individually wrapped

612-3668P2 5550,-

Buy a pack with 50 UV-plates

and get 2 packs of UV-cuvettes

micro free

Description Gap size (mm)

Cat. No. Package price

System HT830/200+Wellplate HT-P96-4–96 4 732-1229P 82500,-








tion cod1

special

package price!

GenoSmart the only gel documentation system you needFor high quality and fast documentation and analysis of your DNA or Protein gels, the VWR Collection is just what you need. Easy to use and very versatile, the unit is compatible with most commonly used electrophoresis gel stains and by using a white or blue light converter on your VWR or other brand of transilluminator (not supplied), you can also capture images from many other applications. Transfer your data onto a USB stick and you can work on images on your PC.

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GenoSmart Gel documentation system 730-3000PP 39640,- 31212,85

Free 20 x 20 cm 312 nm transilluminator

with every purchase

VWR Taq DNA polymeraseIdeal for rich amplificationsBuy 1000 units and get an additional 1000 units completely free!

Contact [email protected] for free samples.

promo code

19

Description Pack size Cat. No. Promo price

VWR Taq DNA Polymerase 1000 units 733-1302P 600,-

�� Real-time images from 8-bit CCD camera (768 x 582 pixel resolution)

�� Self-contained, microprocessor controlled darkroom

�� Integrated colour LCD screen for image viewing

�� White Epi-light�� Instant on-screen saturation

detection�� Rapid photographic print production

0,30 / unit! Take advantage of our GREAT offer on our ultra-pure VWR Taq DNA polymerase.

1

HALF PRICE!



ISSUE 15 - NOVEMBER 2009��Genomics

A thermal cycler that you can afford

The PCR cycler range from the VWR Collection is the solution for the modern molecular biology laboratory, including all the latest features and functionality that will take your research to new level. UNO cycler together with the 96 well standard gradient block is the ideal choice for users who require superb thermal performance in a single headed unit, easy to use, intuitive, powerful.

�� Touch screen�� Gradient�� 2 years warranty

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22

Description Cat. No. Price

Uno Thermal cycler+ 96 well standard gradient block 732-1200PRO1P 35000,-

Great price

*To benefit from this great offer send your details together with your machine’s serial number to [email protected]

Fast & easy enzymatic DNA extraction in 2 steps and less than 30 mins

prepGEM® DNA extraction kits enable rapid extraction of DNA from almost any biological sample in less than 30 minutes and only 2 simple temperature controlled steps. They allow routine genotyping by PCR or qPCR in low to ultra-high-throughput applications without requiring further processing.

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Description Qty/Pk (preps)

Cat. No. Price

prepGEM™ Tissue 50 ZYGEPTI0050P 479,-

200 ZYGEPTI0200P 1700,-

500 ZYGEPTI0500P 3320,-

5,000 ZYGEPTI005KP 26210,-

prepGEM™ Blood 50 ZYGEPBL0050P 479,-

200 ZYGEPBL0200P 1700,-

500 ZYGEPBL0500P 3320,-

5,000 ZYGEPBL005KP 26210,-

prepGEM™ Insect 50 ZYGEPIN0050P 479,-

200 ZYGEPIN0200P 1700,-

500 ZYGEPIN0500P 3320,-

5,000 ZYGEPIN005KP 26210,-

prepGEM™ Storage Card Blood 50 ZYGEPSB0050P 479,-

200 ZYGEPSB0200P 1700,-

500 ZYGEPSB0500P 3320,-

5,000 ZYGEPSB005KP 26210,-

prepGEM™ Storage Card Saliva 50 ZYGEPSS0050P 479,-

200 ZYGEPSS0200P 1700,-

500 ZYGEPSS0500P 3320,-

5,000 ZYGEPSS005KP 26210,-

prepGEM™ Saliva 50 ZYGEPSA0050P 479,-

200 ZYGEPSA0200P 1700,-

500 ZYGEPSA0500P 3590,-

5,000 ZYGEPSA005KP 26210,-

prepGEM™ Bacteria 50 ZYGEPBA0050P 575,-

200 ZYGEPBA0200P 2040,-

500 ZYGEPBA0500P 3980,-

1,000 ZYGEPBA001KP 6730,-

Product features and benefits�� High temperature proteolytic activity results in highly efficient removal of proteins from DNA template giving significantly better PCR

results�� Most preps can be done without centrifugation & don’t require purification�� Highly automatable and efficient yields�� Only 2 liquid handling steps required to perform extraction�� Very limited chemical carryover into the PCR reaction means more of the extraction can be used in a PCR making it ideal for trace samples

Order a free sample by emailing [email protected]

Proteomics

Free Biomarke trolley with every order*

AppliCoat Plate Stabiliser Stabiliser for coated surfaces in immunoassays

�� Preservation of coated microtitre plates, polystyrene beads or glass slides �� Preserves coated plates for 3 months up to 3 years depending on application

AppliCoat Plate Stabiliser can be used on coated ELISA plates, lateral flow assays, protein arrays, and similar applications. AppliCoat Plate Stabiliser seals the plates by forming a uniform stabilising layer over antibodies and antigens immobilised on the solid phase. After incubation of the microtitre plate or solid phase with AppliCoat Plate Stabiliser, the plates may be stored either directly under moist conditions or after drying of the plate or solid phase. The shelf life of the coated molecules is substantially expanded.

* 125ml pack only


AppliCoat Plate Stabiliser Pack A7708.0125P 1020,- 765,-

promo code

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25% OFF*

A high temperature “stress test” at 37 °C for 3 months after application of the plate stabiliser solution. AppliCoat Plate Stabiliser significantly increases the binding activity after storage.



ISSUE 15 - NOVEMBER 2009��Harvest the benefits from using the best in custom biopolymers and in liquid handling

Order Thermo Scientific peptides worth 279 Euro or more and get a Thermo Scientific Finnpipette® F1 30-300 μl plus a rack of Finntips® completely free of charge!!

Conditions: One pipette per person. Offer only valid for orders submitted in electronic format and available as long as stock lasts. Dissemination is at the entire discretion of Thermo Fisher Scientific GmbH. Void when prohibited by law or company / organisation. Offer available in Europe until 31st March 2009.

promo code

24

Having trouble finding a 500 μl horizontal centrifugal device for your protein concentrations? Look from a different angle and go for a vertical design!For decades, Sartorius Stedim Biotech has been helping protein biochemists to concentrate or desalt proteins with patented vertical design Ultrafiltration membrane devices, for volumes from 100 μl to 250 ml. If you are concentrating, desalting or re- buffering volumes of less than 1 ml, Vivaspin 500 is the best choice in the world.

Proteomics

Contact your local VWR LifeSciences specialist for further details.

nefits from using thend in liquid handling

® F1 30-300

cs

Place your peptide orders

until 31st March 2010 mentioning the code VWRF1.

promo code

25

Description MWCO (kDa) Qty/Pk Cat. No. Price Promo price*

Vivaspin 500,Starter PACK

5 of Ea. 5, 10, 30, 50, 100

25 512-3745P 664,- 531,20

Vivaspin 500 3 100 512-2838P 2330,- 1864,-

3 25 512-2837P 664,- 531,20

5 100 512-4001P 2330,- 1864,-

5 25 512-3737P 664,- 531,20

10 100 512-4002P 2330,- 1864,-

10 25 512-3736P 664,- 531,20

30 100 512-4003P 2330,- 1864,-

30 25 512-3738P 664,- 531,20

50 100 512-4004P 2330,- 1864,-

50 25 512-3739P 664,- 531,20

100 100 512-4005P 2330,- 1864,-

100 25 512-3740P 664,- 531,20

300 100 512-4026P 2330,- 1864,-

300 25 512-4025P 664,- 531,20

1000 100 512-4028P 2330,- 1864,-

1000 25 512-4027P 664,- 531,20

0.2 μm 100 512-3744P 2330,- 1864,-

0.2 μm 25 512-3743P 664,- 531,20

See for yourself if this angle works for you! Take advantage of this limited offer and put Vivaspin 500 to the test.

Vertical design means:�� Highest flow rates and recoveries in fast processing times�� Sample integrity with low adsorption, built-in dead-stop design�� Simple, one-step handling, no re-spin necessary, direct pipetting�� Robust and reliable design for minimal membrane blocking

Tech specs: �� Starting volumes from 100

to 500 μl, final volume down to 5 μl

�� Fits standard fixed angle 2.2 ml centrifuge rotors (minimum 25°)

�� Most comprehensive membrane MWCO range in the market

�� 3 kD to 1.000 kD MWCO, plus 0.2 μm low-binding PES membrane

20 % OFF

NAP-BLOCKER™(Non Animal Protein-BLOCKER™)

An animal-free protein blocking agent for immunoassays & ELISAs

NAP-BLOCKER™ is an animal-free protein blocking agent for immunoassays and ELISAs. NAP-BLOCKER™ contains zero proteins of animal origin and can be used as an alternative to sera, BSA, casein or non-fat dry milk to prevent potential interactions between immunoglobulins in these protein solutions and antibodies used for detection. NAP-BLOCKER™ is free from biotin and other cross-reacting agents present in most of the animal source blocking agents, ensuring uniform blocking without non-specific binding. NAP-BLOCKER™ is supplied as a pre-made [2X] solution and is also available as a 2X preparation in PBS or TBS.

A major drawback of other blocking agents, such as BSA, casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the “blocking” animal proteins. NAP-BLOCKER™ ensures no cross-reaction with your animal source antigens and antibodies, due to being 100% free of animal proteins. For improved assay sensitivity, minimal non-specific binding, and a high signal-to-background ratio, NAP-BLOCKER™ is recommended.

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Description Size Cat. No. Price Promo price

NAP-BLOCKER™ [2X]

2 x 500 ml GENO786-190P 1091,- 872,80

NAP-BLOCKER™ [2X] in PBS

2 x 500 ml GENO786-190-PP 1062,- 849,60

NAP-BLOCKER™ [2X] in TBS

2 x 500 ml GENO786-190-TP 1062,- 849,60

20% OFF

Cayman offers a growing selection of antibodiesQuality and affordability: See the Cayman difference

Buy any antibody from Cayman and receive the companion blocking peptide absolutely FREE! Never be caught without the proper controls again. View the full product listing on the VWR LifeScience website

promo code

26

To benefit from this promotion contact: [email protected]

Antibody product lines

Eicosanoid receptors

Eicosanoid synthases and dehydrogenases

Cyclooxygenases and lipoxygenases

PAF Enzymes and phospholipase enzymes

Oxidative injury

Atherosclerosis, lipid & metabolism research

Nitric oxide

Immunology & inflammation

Cell Signalling, apoptosis & cancer research

Neuroscience

FREE blocking

peptide with antibody purchase!



ISSUE 15 - NOVEMBER 2009��

Order from the Customer Service Centre or your VWR Primary Distributor:

These offers cannot be used in conjunction with any other pricing agreements from VWR Interntional. Prices exclude VAT.

New ART® pipette tips reload systemThe only aerosol barrier tip available that guarantees 100% protection against aerosol and liquid contamination of your sample and pipette is now available in environmentally friendly packaging.�� Patented interactive barrier prevents aerosols and aqueous based solutions passing through�� New hinged rack with flip lid offers flexibility by allowing the option of a hinged rack or lift-off lid�� Safe lock latch provides ergonomic one-handed opening and closing�� New reload insert packed in a blister tub for easy insertion�� Certified free of RNase, DNase, ATP and Pyrogens

Supporting productspromo

code30

VWR - Bie & BerntsenTransformervej 82730 Herlev

Place your order today!Tel: 43 86 87 88Fax: 43 86 87 90http://dk.vwr.come-mail: [email protected]

Run the equivalent of 4 standard mini-gels at a time

TWC-202-20 Special Combination Package includes Dual Double-Wide Mini-Vertical system (MGV-202-20), CE with matching 2-place Double-Wide Blotting system (EBU-402), CE. Run or blot two 20 cm(w) x 10 cm(h) gels at a time.

Dual Double-Wide Mini-Vertical system includes:�� Cooling chamber with coolant recirculation ports�� Platinum electrodes�� Long-lasting silicone gasket�� Safety cover with attached retractable sheath power leads,�� White spring clamps�� 2 each 0.75 mm x 17 well combs, 2 glass plate sets, 2 sets of 0.75 mm spacers,

2 Gel Wrap gaskets for casting

Dual Double-Wide Mini-Blotter includes:�� Cooling chamber with internal glass cooling base for temperature controlled

runs�� 2 platinum electrode panels�� 1 gel cassette assembly�� Safety cover with attached power leads�� Tubing adapters

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Mini-Vertical & Blotter Combo Package, CE 730-1347P 8909,52

Thermo Scientific Multiskan FC, a reliable and easy to use microplate photometer

Thermo Scientific Multiskan FC is a new generation microplate photometer designed with 30 years experience in microplate photometry. Multiskan® FC is a reliable and robust instrument for a wide variety of photometric research and routine applications offering ease of use via the large colour display, visual user interface and multiple language options.

* Thermo Scientific Finnpipette F2, 8-channel, 30-300 μl. Thermo Scientific Finntip Flex 300, 10 x 96/rack. Value of the free package 642 EUR.

promo code

28

Proteomics

Purchase a Multiskan FC and

get a multichannel pipette and a box of pipette tips*

FREE!

Special

Offer!


Multiskan FC microplate photometer (405, 450 and 620 nm filters included) 736-0355P 38750,-

Multiskan FC with incubator (405, 450 and 620 nm filters included) 736-0356P 53500,-

Email [email protected] supply us with the serial number of the instrument you purchased, and we will send you your free pipette.

pipette anof pipette

FREE

Type Vol (μl) Qty/Pk Cat. No Price each Promo Price*

Racks

ART-10 REACH 10 10 x 96 732-1140BM 829,- 580,30

ART-20E 10 10 x 96 732-1141BM 829,- 580,30

ART-20P 20 10 x 96 732-1142BM 829,- 580,30

ART-100 100 10 x 96 732-1143BM 829,- 580,30

ART-100E 100 10 x 96 732-1144BM 829,- 580,30

ART-200 200 10 x 96 732-1145BM 829,- 580,30

ART-1000XL 1000 8 x 96 732-1146BM 1040,- 728,-

Reloads

ART-10 REACH 10 10 x 96 732-1148BM 829,- 580,30

ART-20E 10 10 x 96 732-1149BM 829,- 580,30

ART-20P 20 10 x 96 732-1150BM 829,- 580,30

ART-100 100 10 x 96 732-1151BM 829,- 580,30

ART-100E 100 10 x 96 732-1152BM 829,- 580,30

ART-200 200 10 x 96 732-1153BM 829,- 580,30

ART-1000XL 1000 8 x 96 732-1154BM 1040,- 728,-

Buy 3 packs of the ART barrier tips and get a30% discount*

*30% discount for purchase of minimum 3 packs (in racks and/or reloads)

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