E. motion = E. coli + Motion

We will further familiarize people with iGEM and synthetic biology. We are preparing two amazing plans for educational activities for KIT-Kyoto 2015. 

8 Future Plan

1. We conducted a large-scale survey on genetic engineering (n = 1571).2. We constructed new devices that produce monoterpene in E. coli and yeast cells.3. More than 600 people experienced BioArt and genetic engineering through our activities.

7 Conclusion

6 Policy & Practices

Our activities were broadcast on evening news by a Japanese TV station.

Our team members gave lectures on genetic engineering at middle and high schools.

Heated discussion for hours to decide what we should do to raise the awareness of genetic engineering.

We tweeted to communicate with other iGEM teams.

We collaborated with other iGEM teams such as Imperial, Linkoping Sweden, ETH Zurich and Colombia.

We launched wiki specialized for iPhones and Androids.

Hundreds of students enjoyed creating their own artworks with E. motion. 

We gave presentations about genetic engineering and iGEM to high school students and their guardians.

The large-scale survey was conducted in the downtown Kyoto and at our institute.

We designed E. coli and yeast cells which synthesize aromatic substances in orange and lemon such as limonene, pinene and terpinene.We successfully produced γ-terpinene in yeast cells. 

Scent5

LIMS: d-limonene synthase, about 67kDaγTPNS: γ-terpinene synthase, about 65kDa BPINS: β-pinene synthase, about 65kDa GST: GST tag protein, about 27kDa

W e u s e d S P M E ( S O L I D P H A S E M I C R O E X T R A C T I O N ) t o c o l l e c t γ-terpinene at the headspace of the cul ture. However , any amounts of γ-terpinene were not detected at the headspace of the cu l ture (30-min exposure time). Next we collect γ-terpinene in the liquid phase of the culture using monotrap (1-h exposure t ime) and an amount o f γ-terpinene was detected in the liquid phase of the culture.

In 2010, team KIT-Kyoto produced E. coli expressing fluorescent proteins. Hundreds of students experienced BioArt in our institute.

3 Conventional BioArt

Spider Lily

Harvest Moon

Rabbits on the Moon

Larvae's motion is guided by monoterpene, yet it is art of chance unlike any other.  

４ Motion

We adopted BioArt as a powerful tool to familiarize people with genetic engineering. We added MOTION and scent to the conventional BioArt and took it to the next level. E. motion is the BioArt with dynamics and it inspires not only your visual sense but also your olfactory perception. E. motion shakes your emotion!

2 E. motion

Conventional BioArt

MotionScent

NextGeneration

BioArt

Our survey disappointingly indicates that more than 70% of Japanese people think GM foods are enigmatic and have bad images on genetic engineering.

We conducted a large-scale survey on Japanese people's attitudes toward genetic engineering.(n = 1571).

Introduction1

What do you think about genetic engineering?

Positive

Somewhat positive

Somewhat negativeNegative

Not sure

What kind of image do you have on genetic engineering?

Good

Bad

I don't care

Do you feel familiar with genetic engineering?

Yes

No

When you go to a grocery store,which do you buy, GM foods or non-GM foods?

Prefer non-GM foods

Always non-GM foods

Always GM foods

No response

Prefer GM foods

If the price of non-GM foods is 10-20% more expensive than GM foods, what would you do?

Still buy non-GM foods

Buy GM foods

Other

When you hear genetic modification and biotechnology, what comes to your mind? Please select all that apply.

GM foodsCloned animal

iPS cells

Biofuel

Artificial lifeGene therapy

BioethicsBioweapon

ES cells

Other

No response

Which sentence describes your ideas about genetic modification?Please select all that apply.

Unproven safetyPotential effects on future generations

Mysterious

Potential effects on ecosystem

UnethicalNo negative effects on future generations

No response

Other

Do you think that the research of genetic modification should be continued in the future?

Yes

No

Neither of the above

200 larvae were placed at the center of an agar plate (2.0%, radius: 8 cm). A piece of filter paper was placed on each end of the plate (Fig.1). 2.5 µl of monoterpene was dropped on one side of the filter paper (Monoterpene) and cover the plate with a lid. Three minutes later, the number of larvae within a 3 cm radius from the filter paper was counted (A or B) . The concentrations of monoterpene are diluted by 20% and 10% with DMSO. The control is DMSO.

Western blot data (Fig.2) showed clear bands around 90kDa in the lanes of p427-TEF- monoterpenoid synthase but not in the lane of empty vector.

monoterpene

pGEX

Non-mevalonatepathway

IPPDMAPP

pTEF

IPP DMAPP

GPP

Fig.2

GST CuMTS E2/3/62

p427-TEF

KpnⅠ

SpeⅠ XhoⅠ

BamHⅠEcoRⅠSmaⅠSalⅠ

2μ

kanMX

CYC1tTEFp

Ampr

In Yeast

In E. coli

TEF promoter

TAC promoter

TEF

GEX

Cyc1

RBS

Mono-terpenesynthase

Mono-terpenesynthase

Eww...

What should we do?

Our solution is the education through BioArt.

1. We will hold the National BioArt competition in Japan to spread BioArt.

2. We will shoot movies with E. motion and show them to the general public.

Fig.1 Measuring apparatus

AMonoterpene

BControl

Mevalonatepathway

O P O P O-

OO

O-O-

Extra GPP should be added in the medium.

100% Monoterpene Assay

Monoterpene Control

Supported by・Mr. Teppei Hirayabu, advisor of team KIT-Kyoto iGEM 2010

・Dr. Takehiko Shimada, National Agriculture and Food Research Organization in Japan

・COSMO BIO

E. motionIGEM 2014

E.MOTION

- Dawn of the New Generation BioArt -

OW!

3. Laboratory environment will surely improve with the smell of citrus which is famous for aromatics.