Drunken Master - A Seminar on Brewing Technology

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    DrunkenDrunken MasterMaster

    a seminar ona seminar on

    Brewing TechnologyBrewing Technology

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    Introduction: History of BrewingIntroduction: History of Brewing

    Earliest record: 6,000 years old in SumeriaEarliest record: 6,000 years old in Sumeria

    Fermentation discovered by chanceFermentation discovered by chance Hymn to Ninkasi: Sumerian beer recipeHymn to Ninkasi: Sumerian beer recipe

    Beer was a divine drinkBeer was a divine drink

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    Babylonian BeerBabylonian Beer

    Ancient beer was cloudy and unfilteredAncient beer was cloudy and unfiltered

    Beer was used as barterBeer was used as barter

    Hammurabis laws provided for beerHammurabis laws provided for beerrationsrations

    2 L for workers2 L for workers

    3 L for civil servants3 L for civil servants 5 L for administrators and high priests5 L for administrators and high priests

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    The Egyptian BrewThe Egyptian Brew

    Osiris taught humansOsiris taught humans

    to brew beerto brew beer

    Beer residue wasBeer residue was

    found in ancientfound in ancient

    Egyptian vesselsEgyptian vessels

    1996 U of Cambridge1996 U of Cambridge

    analysis showedanalysis showedfruity and sweetfruity and sweet

    beerbeer

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    GrecoGreco--Roman EraRoman Era

    Plinius held that beer came before winePlinius held that beer came before wine

    Romans considered beer as a barbarianRomans considered beer as a barbarian

    drinkdrink Beer was cloudy and produced no foamBeer was cloudy and produced no foam

    People thought of intoxication as divinityPeople thought of intoxication as divinity

    Kalewala (Finnish poetic saga): 400Kalewala (Finnish poetic saga): 400verses for beer; 200 verses for creationverses for beer; 200 verses for creation

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    PrePre--Medieval AgeMedieval Age

    HighHigh--alcohol beer was considered medicinalalcohol beer was considered medicinal

    Brewing vessels were a womans propertyBrewing vessels were a womans property

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    Middle AgesMiddle Ages

    Monasteries took over; scientific brewing beganMonasteries took over; scientific brewing began

    Beer was permitted even while fastingBeer was permitted even while fasting

    Monks moved into brewery salesMonks moved into brewery sales

    Use of hops beganUse of hops began

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    Patrons and WitchesPatrons and Witches

    King GambrinusKing Gambrinus(Flanders and(Flanders andBrabant) was theBrabant) was the

    patron saint of beerpatron saint of beer Hops threatened theHops threatened the

    Grutrecht (flavorGrutrecht (flavorlicense)license)

    Failed brews wereFailed brews wereblamed on beerblamed on beerwitcheswitches

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    ClarificationClarification

    Hops led to beersHops led to beersclear characterclear character

    Wilhelm IV (Duke ofWilhelm IV (Duke of

    Bavaria) proclaimedBavaria) proclaimedthe German Beerthe German BeerPurity Law in 1516Purity Law in 1516

    Industrial RevolutionIndustrial Revolution

    improved temperatureimproved temperaturehandling (steam andhandling (steam andcooling)cooling)

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    Modern BeerIndustry and CommerceModern BeerIndustry and Commerce

    Louis Pasteurs StudiesLouis Pasteurs Studies

    Concerning Beer (1876)Concerning Beer (1876)

    Christian Hansen:Christian Hansen:

    successful isolation of asuccessful isolation of asingle yeast cellsingle yeast cell

    Germany, 1888: SalvatorGermany, 1888: Salvator

    battle (against pricebattle (against priceincrease)increase)

    RP:RP: SSanan MMigueliguel BBeereer

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    MBB: Yeast PhysiologyMBB: Yeast Physiology

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    Traditional Brewing ProcessTraditional Brewing Process

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    Traditional Brewing ProcessTraditional Brewing Process

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    Traditional Brewing ProcessTraditional Brewing Process

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    Traditional Brewing ProcessTraditional Brewing Process

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    Prospects: G.M. Yeast StrainsProspects: G.M. Yeast Strains

    The killer factor versus contaminationThe killer factor versus contamination

    Amylolytic genes for a lowAmylolytic genes for a low--calorie beercalorie beer

    AA--acetolactate decarboxylase gene fromacetolactate decarboxylase gene fromEnterobacter aerogenesEnterobacter aerogenes

    Increasing the copy numberIncreasing the copy numberILV5ILV5 to lessento lessen

    diacetyl productiondiacetyl production

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    Yeast Peptide Complex (YPC)Yeast Peptide Complex (YPC)

    Discovery by Dillemans, et. al. of aDiscovery by Dillemans, et. al. of a

    fermentationfermentation--enhancing peptide complexenhancing peptide complex

    Thesis: Use of proteomic methods such asThesis: Use of proteomic methods such asisolation and characterization for YPCisolation and characterization for YPC

    YPC as a nonYPC as a non--GMO enhancement fromGMO enhancement from

    biotechnological researchbiotechnological research

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    Appreciation of TechnologyAppreciation of Technology

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    End of the PresentationEnd of the Presentation

    Copyright 2002Copyright 2002

    Albert Francis E. DomingoAlbert Francis E. Domingo