Drug target
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DRUG TARGETS AND DRUG DESIGN Ms. Sanchi Dua M. Tech. Biotechnology (2009-11)
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B-Tech.NIET..Introduction to Bio Science By:-Sanchi Dua
Transcript of Drug target
- 1. DRUG TARGETS AND DRUG DESIGN Ms. Sanchi Dua M. Tech. Biotechnology (2009-11)
- 2. Introduction: Alzheimer's Disease Progressive Neurodegenerative Disorder Acute Cognitive Decline Neuronal Cell Dysfunction Memory Loss Poorly understood
- 3. Amyloid Beta (A) A (betaamyloid peptide) Hallmark of Alzheimer's disease (AD) Yeast Cells used to study harmful effects
- 4. Pathological Characterization Accumulation of extracellular beta amyloid (A) plaques Intracellular tau tangles Gliosis Neuronal cell death
- 5. Research Objective Identify biomarkers Peripheral Biomarkers Blood samples easy to obtain Novel cognitive- Increased Efficacy and Safety enhancing therapeutic strategies
- 6. Existing clinical methods of diagnosis Brain Imaging Characterizing fluid biomarkers Cerebrospinal fluid (CSF)
- 7. Blood-based biomarkers Potential sources Platelets Small, anuclear fragments derived from megakaryocytes in the bone marrow Platelets Respond to neurotransmitters Enzymes responsible for generating the A peptide. High concentration of amyloid precursor protein (APP)
- 8. Methodology for identifying Biomarkers Validation of Cooccurrence of other pooled analyte changes consistent with previous biomarker studies Platelet isolation and membrane protein enrichment Quantification of membrane-enriched proteome
- 9. Cognitive Aging Aging Cognitive functions decline
- 10. Current drug targets To improve cognitive function Inhibit acetylcholinesterase (AChE) N-methyl-D-aspartate receptor
- 11. Novel cognitive-enhancing therapeutic strategies To slow disease progression in AD patients To enhance cognitive function
- 12. Potential Drug Targets Metabotropic glutamate receptor subtype 5 Increasing the activation of mGlu5 Nicotinic acetylcholine receptor Activation of the 7nAChR subtype with smallmolecule agonists M1 Muscarinic acetylcholine receptors potential to affect multiple disease pathologies including amyloid-beta and phosphorylated tau
- 13. Potential Drug Targets Indirect cholinergic 5Hydroxytryptamine stimulation through 5-hydroxytryptamine receptors (5-HT) receptors 6 Norepinephrine Noradrenergic neurons: vulnerable during aging Cyclic adenosine monophosphate cAMP signaling is increased with aging
- 14. Experimental Techniques Immunostaining Immunoblot Indirect ELISA Assay Mass spectrometry (MS)-based proteomics