Dr. Graham ScolesDr. Brian Rossnagel

Crop Molecular Genetics LabDepartment of Plant Sciences

Barley and Oat Breeding ProgramCrop Development Centre

PersonnelDr. Aaron BeattieDr. Tajinder GrewalDonna HayPeter Eckstein

Marker Discovery, Development and Implementation

Molecular Marker-Assisted Selection

Genomics

Oat (and Barley)Traditional polymorphism screen using a variety of fingerprinting methods and linkage analysis• usually for simply inherited traits• usually using bi-parental populations• polymorphisms developed for ease of use in MMAS• implement/adapt markers developed by other programs

Research Objectives

Marker implementation in breeding materials on a routine basis - lower cost - increased accuracy - shortened breeding cycle

Oat markers include/d; Pg16, loose smut, Pc94, Pc68

• EST Isolation• SSR marker development• TILLING collaboration• DArT Consortium• QTL analysis, association mapping/linkage dis-equilibrium

Marker-Assisted Selection

PCR based markers - 2 primer allele-specific or SCAR- 96 lane agarose gel electrophoresis

Rapid and crude DNA preparation - sodium hydroxide based- germinated seed- 96 samples PCR ready in 45 minutes

Where to Next ??

Too many samples for a research labToo few for a dedicated high-throughput facility

Critical Mass – need more marked traits - need quality markers - have barley Un8, Cov. Smut, Yd2, Pc11, Rh2, Rh13,

Rpg1, Net Blotch, low-protein, LOX1 - have lost Pc68, Rh2, Pc11, chr. 3 scald R.

SNP Markers

Variety of ways to implement and analyse

Barley CAP leading the way- great for genotyping, ease marker discovery- markers from the shelf - too poor - pick a SNP

- too far - pick a SNP - not poly - pick a different one - wrong marker type - select SNP from region

Oat - perhaps a little behind barley

Real-Time PCR - Cybergreen or TaqMan