Dr Conrad Introduction to Microfluidics

7/27/2019 Dr Conrad Introduction to Microfluidics

1/48

/)75@:/*/)8,57).+3/)'2'4*

(/525-/)'2+4-/4++7/4-

Jacinta C. Conrad

University of Houston

Chemical and Biomolecular Engineering

CHEE 1131

Fall 2013

1Shepherd, Conrad, Lewis, et al., Langmuir22, 86188622 (2006)


2/48

2

"=56.?9/)'26856+8:/+95,I;/*9

B Viscosity3+'9;8+5,I;/*8+9/9:'4)+:59:8+99%3'992+4-:.:/3+&

B +49/:?%3'992+4-:.3&

9A61=5E?60.912J;6@6


3/48

3

#.0>


4/48

)52>21


5/48

Flow examples in plants (unit operations)

5

53(/4':/543/>/4-56+8':/54:5)8+':+'.535-+4+5;99?9:+3

+7;/8+9)54:8525/4-9:8+'39

!+6'8':/549+6'8':/545,3/>:;8+)53654+4:9

3;29/H)':/54)8+':/545,'2/7;/*/42/7;/*9;96+49/54

/9:/22':/549+6'8':/545,54+2/7;/*,853'45:.+82/7;/*


6/48

Flow examples in plants (unit operations)

2;/*I5=685)+99+9+-H2:8':/54I;/*:8'49658:

+'::8'49,+8685)+99+9+-+:8'):/54'*9586:/54*8?/4-

".+835*?4'3/)685)+99+9+--'92/7;+,'):/548+,8/-+8':/54 +).'4/)'2685)+99+9+-9)8++4/4-'4*9/+


7/48

#60>6F.@6


8/48

"2;4@5?0.92?3:60>


9/48

#.@2>6.9?3:60>


10/48

#.@2>6.9?3:60>6461=9.?@60?

X

'/7et al.Lab Chip

*


11/48

#.@2>6.9?3:60>


12/48

#.@2>6.9?3:60>


13/48


14/48

>[email protected]@62?6;12B602?

10

=

V L

+?452*94;3(+8+

/4+8:/'2,58)+


15/48

#60>


16/48

>[email protected]@62?6;12B602?

12

=

V L

+?452*94;3(+8+

/4+8:/'2,58)+

/4-8+7;/8+996+)/'2*+


17/48

j = D0dc

dx

D0 =B

6a

/4-8':+/43/)85I;/*/)*+5,*/,,;9/54

species concentration [kg/m3]KAD


18/48

Combination: passive micromixer

X

+?/*+'4)8+'9+:.+2+4-:.5,:.+I5=).'44+2

http://www.youtube.com/

C.@05B+#>@;%?


19/48

Combination: passive planar micromixer

13

Melin et al., Lab Chip, 214-219 (2004)

+?/*+'5*/,?-+53+:8?:55(:'/43/>/4-


20/48

Combination: parallel lamination mixer

14

+?/*+'!62/:9:8+'39:5/4)8+'9+9;8,')+'8+''4*.+4)+3/>/4-

!.:5


21/48

Combination: 3-D serpentine mixers

X

+?/*+'**+2+3+4:9:5F,52*GI;/*


22/48

b

a

c

Combination: 3-D microvascular networks

15

+?/*+'!62/:9:8+'39/4*-+53+:8/+9:5+4.'4)+3/>/4-

'52>>6.A9@et al., Nat. Mater.2, 265-271 (2003)


23/48

Combination: herringbone micromixers

16

+?/*+'**+2+3+4:9:5F,52*GI;/*


24/48

Combination: herringbone mixer movie

1752).2'("4/;+78/9=5,#'8./4-954;/'%5:!:(+


25/48


26/48


27/48

Combination: colloid valves

19

+?/*+'4)58658':+3/)8549/@+*)5225/*'26'8:/)2+9/4:5*+>.Eet al., Science, 1841-1844 (2002)


28/48


29/48

&2=.>.@6


30/48

:A9?6J0.@6


31/48

>


32/48

:A9?6J0.@6/4-8':+9


33/48

:A9?6J0.@6


34/48

>


35/48

Separation: cell sorting via optical forces

27

Waste Collection

Optical switch

Analysis region

BufferBuffer

Sample input

+?/*+'#9+8'*/':/5468+99;8+:5958:)+229/4'3/)85I;/*/)*+


36/48


37/48

Separation: deterministic lateral displacement

28

+?/*+''8:/)2+95,*/,,+8+4:*/'3+:+8,5225=*/,,+8+4:9:8+'32/4+9

Huang et al., Science 987-990 (2004)


38/48

Separation of parasites from blood

29Holm et al., Lab Chip, 1326-1332 (2011)


39/48

Separation: motile sperm sorter

30

+?/*+'/


40/48

Separation: distillation

31

+?/*+'9:'(2/9.


41/48

Reaction: drops as microreactors

32

+?/*+'8569/4)8+'9+8+'):/548':+9(?/4)8+'9/4-9;8,')+:5


42/48

%2.0@6


43/48

Reaction: nucleation

34

Chen et al., J. Am. Chem. Soc. 9672-9673 (2005)

+?/*+'+9/-4'*8562+:('9+*3/)85I;/*/)9?9:+3:59:;*?+,,+):5,

3/>/4-544;)2+':/545,685:+/4)8?9:'29


44/48

Reaction: nanoparticle synthesis

35

+?/*+'#9+5,-'992;-9:59+6'8':+93'222/7;/*8+'):/54


45/48

%2.0@6?E;@52?6?

36

3/)85:;(;2+9

3/)85H(+89

Khan et al., Lab Chip 576-580 (2004)

+?/*+'.5:5652?3+8/@+'I5=,5);9+*9:8+'3F54:.+I?G

R i di hi


46/48

Reaction: gradient etching

37

+?/*+'8'*/+4:9/48+'):'4:)53659/:/54-+4+8':+*/,,+8+4)+9/4+:)./4-

8':+9:.85;-.'9;8,')+

Jeon et al., Langmuir 8311-8316 (2000)

96 6 3 6 K 616


47/48

==960.@6/9'4*).+3/)'28+96549+

96 @6 @ 996 @6


48/48

iii

i ii ii

iv iv

v vi

iii

i ii

iv

v

i

iii

Protein

Fluorinatedcarrier uid

PDMSGlasscapillary

Precipitant

Buer

aValve-based system

bDroplet-based system

cSlipChip (well-based) system

Open

Open

Valve

Loadprecipitant

Loadprotein

Close

Close

Close

Mix

1 mmmm

100 m00 m

1 mmmm 1 mm

1 cm

100 m

1 mm

100 m

100 m

250 m

Load protein

Preloadedprecipitants

Mix proteinwith precipitants

Slip

SlipProtein

Slip

Slip into contact

Precipitants

Protein

Crystallization trials

250 m

Open

==960.@[email protected].@6

Dr Conrad Introduction to Microfluidics

Documents

Transcript of Dr Conrad Introduction to Microfluidics