Download preliminary programme (pdf) - Events
Transcript of Download preliminary programme (pdf) - Events
Marine animal models in evolution & development 1 – 12 July 2013 | Fiskebäckskil, Sweden
Preliminary Agenda PIs lectures: each PI has 1 hour slot that should include 45 minutes lecture + 15 minutes discussion. Please stick to this schedule. Sweatbox: after the lecture, all other PIs leave the room while students ask questions to the speaker for about half an hour. SUNDAY JUNE 30, 2013 - ARRIVAL DAY
17:30 - 19:00 Welcome buffet
19:00 - 20:00 Welcome and introduction to the course - Detlev Arendt
20:00 – 21:00 Keynote lecture by Claus Nielsen: Marine life at Gulmarsfjord
MONDAY JULY 1, 2013 07.30 – 08.30 BREAKFAST
Participants split in 4 working groups (WG)
08.30 – 12.30 WG 1 and 2 Boat trip: plankton, benthic meio- and macrofauna collection
WG 3 and 4 Safety briefing Plankton collection by teachers
12.30 – 13.30 LUNCH
13.30 – 17.30 WG 3 and 4 plankton inspection WG 1 and 2 safety briefing
Inspection of plankton and benthic meio- and macrofauna Collection of plankton with rowing boat
17.30 – 18.30 DINNER
18.30 – 19.30 Wrap up by Claus Nielsen
19.30 – 21.00 Poster Session
TUESDAY JULY 2, 2013 07.30 – 08.30 BREAKFAST
08.30 – 12.30 WG 3 and 4 Boat trip: plankton, benthic meio- and macrofauna collection
WG 1 and 2 Plankton collection with rowing boats
12.30 – 13.30 LUNCH
13.30 – 14.00 Introduction to comparative antibody staining (Ina Arnone)
14.00 – 15.00 Introduction to 4D microscopy/SIMI BioCell rotifer, acoel, tardigrade, gastrotrich (Andreas Hejnol & Sabrina Schiemann)
15.00 – 15.30 Introduction to microinjections, cell lineaging and 4D microscopy on Parhyale (Frederike Alwes)
15.30 – 17.30 Inspection of plankton and benthic meio- and macrofauna
Collection of plankton with rowing boat
Fixation of samples Start of antibody staining
17.30 – 18.30 DINNER
18.30 – 19.00 Introduction to CLEM (Harald Hausen and Gaspar Jékély)
19.00 – 20.00 Lecture: Phylogeny reconstruction and phylogeny interpretation (Max Telford)
20.00 – 20.30 “Sweatbox” (Max Telford)
20.30 – 21.00 Introduction to Light Sheet Fluorescence Microscopy and Open SPIM (Olaf Selchow and Pavel Tomancak)
WEDNESDAY JULY 3, 2013 08.00 – 09.00 BREAKFAST
09.00 – 09.30 Introduction to 2 color fluorescent in situ hybridization (Ina Arnone/Evelyn Houliston)
09.30 – 10.00 Introduction to comparative in situ staining (Heather Marlow)
10.00 – 10.30 Introduction to the flatworm Maritigrella (Bernhard Egger)
10.00 – 12.00 Antibody staining LSFM 4D microscopy Microinjections / Cell lineaging Collected samples
fixation and preparation
12.00 – 13.00 LUNCH
13.00 – 17.30 Antibody staining LSFM 4D microscopy Microinjections / Cell lineaging Collected samples
fixation and preparation
17.30 – 18.30 DINNER
19.00 – 20.00 Lecture (Ina Arnone)
20.00 – 20.30 Sweatbox (Ina Arnone)
THURSDAY JULY 4, 2013 08.00 – 09.00 BREAKFAST
09.00 – 09.30 Introduction to Nematostella vectensis (Heather Marlow)
09.30 – 10.00 Introduction to Clytia hemisphaerica (Evelyn Houliston)
10.00 –12.00 Antibody staining In situ hybridization (WMISH)
Microinjections / Cell lineaging
4D microscopy LSFM
12.00 – 13.00 LUNCH
13.00 – 17.30 Antibody staining In situ hybridization (WMISH)
Microinjections / Cell lineaging
4D microscopy LSFM
17.30 – 18.30 DINNER
18.30 – 19.30 Lecture (Detlev Arendt)
19.30 – 20.00 Sweatbox (Detlev Arendt)
20.00 – 21.00 Lecture (Gaspar Jékely)
21.00 – 21.30 Sweatbox (Gaspar Jékely)
FRIDAY JULY 5, 2013 08.00 – 09.00 BREAKFAST
09.00 – 09.30 Introduction to sea urchins (Ina Arnone)
09.30 – 10.00 Introduction to Platynereis dumerilii (Detlev Arendt)
10.00 – 12.00 In situ hybridization (WMISH) Microinjections / Cell lineaging 4D microscopy
LSFM
12.00 – 13.00 LUNCH
13.00 – 14.00 Lecture: Confocal laser scanning and reflection microscopy techniques demo (Gaspar Jékely)
13.30 – 17.30 In situ hybridization (WMISH) Microinjections / Cell lineaging 4D microscopy
LSFM
17.30 – 18.30 Lecture on fossils (Graham Budd)
18.30 – 19.00 Sweatbox (Graham Budd)
19.00 – 19.30 Introduction to field trip and fossils (Graham Budd)
20:00 - BBQ Bag packing for trip
The field-trip is organized and guided by Graham Budd, professor of Paleontology at Uppsala University.
More information on the field trip will be distribute soon.
SATURDAY JULY 6: Paleontological Field Trip SUNDAY JULY 7: Paleontological Field Trip
MONDAY JULY 8, 2013 08.00 – 09.00 BREAKFAST
09.00 – 09.30 Introduction to transgenic tools in emerging models (Michalis Averof)
09.30 – 10.00 Introduction to image registration (Gaspar Jékely)
09.30 – 12.00 Microinjections / cell lineaging
Finish antibody staining
Finish in situ hybridization (WMISH)
4D microscopy / LSFM
Image registration
12.00 – 13.00 LUNCH
13.30 -- 17.30 Microinjections
17.30 – 18.30 DINNER
18.30 – 19.00 Introduction to Ascidians and electroporation (Patrick Lemaire)
19.00 – 20.00 Lecture (Evelyn Houliston)
20.00 – 20.30 Sweatbox (Evelyn Houliston)
20.30 -- Night sampling (full moon)
TUESDAY JULY 9, 2013 08.00 – 09.00 BREAKFAST
09.00 – 09.30 Introduction to microinjections in several species and lineaging by tracer dye injections (Mark Q. Martindale)
09.30 – 10.00 Introduction to cell lineaging in Ophiuroids (Paola Olivieri)
10.30 – 12.00 Microinjections/cell lineaging
Transgenic tools in Parhyale Electroporation Confocal / LSFM Image registration
12.00 – 13.00 LUNCH
13.00 – 17.30 Microinjections/cell lineaging
Transgenic tools in Parhyale Electroporation Confocal / LSFM Image registration
17.30 – 18.30 DINNER
19.00 – 20.00 Lecture (Pavel Tomancak)
20.00 – 20.30 Sweatbox (Pavel Tomancak)
20.30 – 21.30 Lecture (Michalis Averof)
21.30 – 22.00 Sweatbox (Michalis Averof)
WEDNESDAY JULY 10, 2013
08.00 – 09.00 BREAKFAST
09.00 – 10.00 Lecture (Harald Hausen)
10.00 – 10.30 Sweatbox (Harald Hausen)
10.30 – 12.00 Microinjections/cell lineaging
Transgenic tools in Parhyale Electroporation Confocal / LSFM Image registration
12.00 – 13.00 LUNCH
13.00 – 17.30 Microinjections/cell lineaging
Transgenic tools in Parhyale Electroporation Confocal / LSFM Image
registration
17.30 – 18.30 DINNER
18.30 – 19.30 Lecture (Mark Q. Martindale)
19.30 – 20.00 Sweatbox (Mark Q. Martindale)
20.00 – 21.00 Lecture (Patrick Lemaire)
21.00 – 21.30 Sweatbox (Patrick Lemaire)
THURSDAY JULY 11, 2013 08.00 – 09.00 BREAKFAST
09.00 – 12.00 Microinjections/cell lineaging
Transgenic tools in Parhyale Electroporation Confocal / LSFM Image
registration
12.00 – 13.00 LUNCH
13.00 – 17.30 Finalizing all ongoing experiments
17.30 – 18.30 DINNER
18.30 – 19.30 Lecture (Andreas Hejnol)
19.30 – 20.00 Sweatbox (Andreas Hejnol)
FRIDAY JULY 12, 2013 08.00 – 09.00 BREAKFAST
09.00 – 12.00 Studentsʼ presentations and discussions
12.00 – 13.00 LUNCH
13.00 – 17.30 Students presentations and discussion
17.30 – 18.30 DINNER
18.30 – 19.30 Course wrap-up Feedback session
20.00 onwards PARTY!
SATURDAY JULY 13, 2013 08.30 – 09.30 BREAKFAST
Departures