Electronic Supplementary Material

Solid phase extraction of antidepressant drugs amitriptyline and nortriptyline from plasma

samples using core-shell nanoparticles of the type Fe3O4@ZrO2@N- cetylpyridinium, and

their subsequent determination by HPLC with UV detection

Fahimeh Zare1, Mehrorang Ghaedi1,*, Ali Daneshfar2

1 Department of chemistry, Faculty of sciences, Yasouj University, Yasouj 75918-74831, Iran

2 Department of chemistry, Faculty of sciences, Ilam University, Ilam 69315-516, Iran

*Corresponding author: Tel./Fax: + 98 743 222 3048, E-mail: m_ghaedi@yahoo.com

Figure (S1). FT-IR spectra of Fe3O4 ; Fe3O4@ZrO2 and Fe3O4@ZrO2@N-cetylpyridinium -

NPs.

Figure (S2). TGA plot of Fe3O4@ZrO2@ N-cetylpyridinium -NPs.

Figure (S3). Effect of pH on magnetic hemimicelle solid phase extraction (MSPE) extraction

efficiency (n=3).

Figure (S4). Effect of type of surfactant on MSPE extraction efficiency (n=3).

Figure (S5). Effect of amount of surfactant on MSPE extraction efficiency (n=3).

Figure (S6). Effect of type of eluent solvent on MSPE extraction efficiency (n=3).

Figure (S7). Standardized main effect Pareto chart for the Plackett-Burman design of

screening experiment. Vertical line in the chart defines 95 % confidence level.

Figure (S8). Response surface for the 22 central composite designs: amount of nanoparticle-

sample volume.

Figure (S9). The optimization plot for (a) Plackett-Burman design (b) central composite design

including seven factors.