DNA Prepared from ATCC Genuine Cultures™

Your Discoveries Begin With Us.®

Genomic DNA from Bacteria & ArchaeaATCC® No. Organism Strain Type Strain Sequenced Genome

ATCC offers purified DNA from well-characterized and authenti-cated microbial strains and molecularly cloned viruses. Genomic DNA from ATCC Genuine Cultures™ are available from:

• Bacteria • Mycoplasma • Protozoa • Fungi and yeast

Genomic DNA from ATCC Genuine Cultures™ can save you the time and expense of isolating DNA yourself. Applications include:

• Construction of genomic libraries • Southern hybridization • PCR • Identification and comparison at the genus, species, and strain levels

The package size of Genomic DNA prepared from bacteria, fungi and yeast is in the process of being changed. Genomic DNA will be available only in a 5-µg package size. The 10-µg size is discon-tinued when the 5-µg size becomes available. The ATCC catalog number will change to add ‘-5’ as a suffix. For example: ATCC

DNA Prepared from ATCC Genuine Cultures™

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catalog number 10556D has been changed to 10556D-5 for the new 5-µg size. The package size of DNA prepared from Mycoplasma remains unchanged at either 10-ng or 50-ng.

We’ve expanded our quality control testing for the 5-µg size to include the determination of the total amount of DNA by PicoGreen® measurement. This high-quality DNA has been isolated under aseptic conditions to prevent cross-contamina-tion. Batches are evaluated for integrity, purity and quality by several methods, including:

• Agarose gel electrophoresis• Spectrophotometry• Suitability for amplification by PCR • Sequencing of the 16S ribosomal RNA gene (first ~ 500 base pairs) consistent with the sequence of the source organism for bacteria • ITS sequencing for fungi and yeast consistent with the sequence of the source organism for fungi or yeast

Genomic DNA available in the 5-µg size that has been tested using the additional PicoGreen® measurement is shaded in GREEN on the list below.

23206D Acholeplasma laidlawii PG8 Y 14780D Achromobacter xerosis IMRU 134 Y 13598D Acidithiobacillus ferrooxidans 23270D Acidithiobacillus ferrooxidans NCIB 8455 Y 17925D Acinetobacter lwoffii NCIB 9020 49467D Acinetobacter sp. AmMS 248 27088D-5 Actinobacillus pleuropneumoniae 4074 Y 12104D Actinomyces naeslundii 279 Y 700406D Aerococcus viridans API 78 12-095 7965D Aeromonas hydrophila NCTC 7812 700893D-5 Aeropyrum pernix JCM 9820 Y Y8750D Alcaligenes faecalis subsp. faecalis 16 Y 19411D Arcanobacterium pyogenes NCTC 5224 Y 49558D Archaeoglobus fulgidus DSM 4304 Y Y10987D-5 Bacillus cereus NRS 428 Y14579D Bacillus cereus 971 Y Y21591D Bacillus halodurans A-59 BAA-125D-5 Bacillus halodurans C-125, JCM 9153 Y23857D-5 Bacillus subtilis 168 82D Bacillus subtilis AMC 33679D Bacillus thuringiensis NRRL B-3792 35646D Bacillus thuringiensis USDA HD522 25285D Bacteroides fragilis VPI 2553; NCTC 9343 Y Y29148D-5 Bacteroides thetaiotaomicron VPI 5482 Y Y43606D-5 Bacteroides ureolyticus P2N12 8482D Bacteroides vulgatus NCTC 11154 Y

ATCC® No. Organism Strain Type Strain Sequenced Genome

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49882D-5 Bartonella henselae Houston-1 Y Y15703D Bifidobacterium adolescentis E194a (Variant a) Y Y15700D Bifidobacterium breve S1 (Variant a) Y 15697D Bifidobacterium infantis S12 Y BAA-588D-5 Bordetella bronchiseptica RB50 Y15311D-5 Bordetella parapertussis NCTC 5952 Y BAA-587D-5 Bordetella parapertussis 12822 Y9797D-5 Bordetella pertussis 18323 Y BAA-589D Bordetella pertussis Tohama 1 Y35210D-5 Borrelia burgdorferi B31 Y Y9175D Brevibacterium linens KC BAA-1061D Campylobacter coli 33292D-5 Campylobacter jejuni subsp. jejuni AS-84-79 33560D-5 Campylobacter jejuni subsp. jejuni CIP 702 Y 43432D-5 Campylobacter jejuni subsp. jejuni MK7 43446D-5 Campylobacter jejuni subsp. jejuni MK104 700819D-5 Campylobacter jejuni subsp. jejuni NCTC 11168 YBAA-1234D-5 Campylobacter jejuni subsp. jejuni RM3193 43431D-5 Campylobacter jejuni subsp. jejuni TGH 9011 43503D-5 Campylobacter jejuni subsp. jejuni UA466 BAA-1060D Campylobacter lari 19089D Caulobacter vibrioides CB 15 Y49652D Chlorobium tepidum TLS Y Y12472D Chromobacterium violaceum MK Y Y8090D Citrobacter freundii NCTC 9750 Y 824D-5 Clostridium acetobutylicum Y Y9689D-5 Clostridium difficile 90556-M6S Y 14880D Clostridium glycolicum Y 13124D-5 Clostridium perfringens Y Y3584D Clostridium sporogenes 388 Y 27405D Clostridium thermocellum VPI 7372 Y YBAA-681D Colwellia psychrerythraea 34H Y700971D-5 Corynebacterium diphtheriae NCTC 13129 Y33798D Corynebacterium genitalium 205E 13032D-5 Corynebacterium glutamicum 534 Y7711D Corynebacterium xerosis CNF 010 43123D-5 Cupriavidus metallidurans CH34 Y Y33406D-5 Cytophaga hutchinsonii NCIB 9469 Y Y13939D-5 Deinococcus radiodurans RI (smooth) Y BAA-816D Deinococcus radiodurans USUHS Y15995D Derxia gummosa III 43914D Desulfobacterium autotrophicum DSM 3382 Y 33405D Desulfovibrio vulgaris subsp. oxamicus NCIB 9442 Y 29579D-5 Desulfovibrio vulgaris subsp. vulgaris NCIB 8303 Y Y51724D Eikenella corrodens CCUG 29131 51720D Elizabethkingia meningoseptica CCUG 26117 33958D Elizabethkingia miricola 15038D Enterobacter aerogenes IFO 12010 13047D-5 Enterobacter cloacae NCDC 279-56 Y 23373D Enterobacter dissolvens ICPB ED105 Y 9912D Enterobacter nimipressuralis 41-173 Y 49603D Enterococcus avium NCDO 2370 700802D-5 Enterococcus faecalis V583 Y51559D-5 Enterococcus faecium MMC4 BAA-472D-5 Enterococcus faecium TEX16 49712D Erysipelothrix rhusiopathiae CJPT-97 10798D-5 Escherichia coli K-12 700926D-5 Escherichia coli MG1655 Y700928D-5 Escherichia coli CFT073 Y

700927D* Escherichia coli 0157:H7 EDL 933 YBAA-460D* Escherichia coli 0157:H7 RIMD 0509952, Sakai Y8486D Eubacterium limosum NCIB 9763 Y 49145D Gardnerella vaginalis AmMS 117 10379D Gemella haemolysans NCTC 5414 Y 12980D-5 Geobacillus stearothermophilus NCA 26 Y 53774D-5 Geobacter metallireducens GS-15 Y Y51573D-5 Geobacter sulfurreducens PCA Y Y700685D-5 Haemophilus actinomycetemcomitans HK1651 700724D-5 Haemophilus ducreyi 35000HP Y51907D Haemophilus influenzae KW20 Rd Y700560D Halanaerobium lacusrosei H200 Y 700875D Haloarcula argentinensis DSM 12282 Y 43049D-5 Haloarcula marismortui DSM 3752 Y Y700850D Haloarcula quadrata DSM 11927 Y 33170D Halobacterium salinarum NRC 34001 Y 33171D Halobacterium salinarum NRC 34002 Y 700922D Halobacterium sp. NRC-1 Y700876D-5 Halobaculum gomorrense DSM 9297 Y 29605D Haloferax volcanii DS2 Y 43504D-5 Helicobacter pylori NCTC 11637 Y 700392D-5 Helicobacter pylori 26695 Y700824D-5 Helicobacter pylori J99 Y33394D Kingella denitrificans NCTC 10995 Y 23332D Kingella kingae 5530 700324D Klebsiella oxytoca LBM 90.11.033 700721D-5 Klebsiella pneumoniae subsp. pneumoniae MGH78578 4357D Lactobacillus acidophilus Pak 8287D Lactobacillus brevis 269Y 7995D Lactobacillus delbrueckii subsp. bulgaricus USDA Lb7 BAA-365D Lactobacillus delbrueckii subsp. bulgaricus Lb-18 Y8000D Lactobacillus delbrueckii subsp. lactis 39 E/K 25258D Lactobacillus jensenii 62G Y BAA-793D Lactobacillus plantarum NCIMB 8826, WCFS1 Y19435D Lactococcus lactis subsp. lactis NCTC 6681 Y 33152D-5 Legionella pneumophila subsp. pneumophila Philadelphia-1 Y YBAA-680D Listeria innocua CLIP 11262 Y19115D Listeria monocytogenes Li 2 BAA-409D Mannheimia haemolytica SH1217 BAA-410D Mannheimia haemolytica SH789 43067D-5 Methanocaldococcus jannaschii DSM 2661 Y YBAA-1333D-5 Methanococcus maripaludis C5 YBAA-1332D-5 Methanococcus maripaludis C6 BAA-1331D-5 Methanococcus maripaludis C7 43000D Methanococcus maripaludis DSM 2067 Y BAA-1334D-5 Methanococcus voltae A3 BAA-159D Methanosarcina mazei DSM 3647, Go1 Y51484D Methylobacillus flagellatus KT Y Y14718D-5 Methylobacterium extorquens NCIB 9133 27886D-5 Methylobacterium organophilum XX Y 19069D-5 Methylococcus capsulatus Y 49242D Methylocystis sp. AS1 33003D Methylomicrobium album NCIB 11123 Y 53528D Methylophilus methylotrophus Rockwell Y 53598D Micrococcus luteus SC 14641 25240D Moraxella (Branhamella) catarrhalis N9 17967D Moraxella (Moraxella) lacunata CDC KC 784 Y 19976D Moraxella (Moraxella) osloensis A1920 Y 35200D Morganella morganii subsp. morganii AM-15 35760D-5 Mycobacterium gordonae TMC 1327 23037D-5 Mycobacterium smegmatis 1102 19015D-5 Mycobacterium sp. BCG

ATCC® No. Organism Strain Type Strain Sequenced Genome

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23838D Mycoplasma arginini G230 Y 19611D Mycoplasma arthritidis Preston Y 19989D Mycoplasma fermentans PG18 Y 33530D Mycoplasma genitalium G37 Y Y23114D Mycoplasma hominis PG21 Y 17981D Mycoplasma hyorhinis BTS-7 Y 23714D Mycoplasma orale CH 19299 Y 25960D Mycoplasma pirum Guidice HRC/70-159 Y 15531D Mycoplasma pneumoniae FH strain of Eaton Agent Y 29342D Mycoplasma pneumoniae M129 Y23064D Mycoplasma salivarium PG20 Y 14221D Neisseria flava 53420D Neisseria gonorrhoeae 53421D Neisseria gonorrhoeae 53422D Neisseria gonorrhoeae 53423D Neisseria gonorrhoeae 53424D Neisseria gonorrhoeae 53425D Neisseria gonorrhoeae 700825D Neisseria gonorrhoeae FA1090 Y53414D-5 Neisseria meningitidis Serogroup C 53415D-5 Neisseria meningitidis Serogroup B 53416D Neisseria meningitidis 53417D-5 Neisseria meningitidis Serogroup A M1027 53418D Neisseria meningitidis 53419D Neisseria meningitidis 700532D-5 Neisseria meningitidis FAM18 YBAA-335D-5 Neisseria meningitidis MC58 Y14799D-5 Neisseria perflava 28 19718D-5 Nitrosomonas europaea OR74A Y29133D-5 Nostoc sp. PCC 73102 700278D-5 Novosphingobium aromaticivorans F199, DSM 12444 Y YBAA-331D Oenococcus oeni PSU-1 Y33243D Pantoea agglomerans NCPPB 2971 14536D Pantoea ananatis AJ 2189 19321D Pantoea ananatis NCPPB 800 Y 8199D Pantoea stewartii subsp. stewartii SS 11 Y BAA-672D Pectobacterium atrosepticum SCRI 1043 Y49031D Peptostreptococcus anaerobius MSHD 51903D Plesiomonas shigelloides GNI 14 33277D-5 Porphyromonas gingivalis 2561 Y BAA-308D-5 Porphyromonas gingivalis W83 Y25845D-5 Prevotella melaninogenica VPI 2381 Y 25746D-5 Propionibacterium acnes D-34 12453D Proteus mirabilis D1 9920D Proteus vulgaris 33672D Providencia stuartii 495 17933D Pseudomonas aeruginosa 633 47085D-5 Pseudomonas aeruginosa PA01-LAC YBAA-477D Pseudomonas fluorescens Pf-5 Y47054D-5 Pseudomonas putida KT2440 Y11355D Pseudomonas syringae pv. phaseolicola 3035 BAA-978D Pseudomonas syringae pv. phaseolicola 1448A YBAA-871D-5 Pseudomonas syringae pv. tomato DC3000 Y43587D-5 Pyrococcus furiosus DSM 3638 Y Y700860D-5 Pyrococcus horikoshii JCM 9974 Y Y49646D Rahnella aquatilis SAL 11696D-5 Ralstonia solanacearum CH34 Y 33970D Rhizobium radiobacter C58 YBAA-846D Rhizobium vitis S4 BAA-309D Rhodobacter capsulatus SB1003 17023D Rhodobacter sphaeroides ATH 2.4.1 Y BAA-808D Rhodobacter sphaeroides 2.4.1 YBAA-98D-5 Rhodopseudomonas palustris CGA009 Y

ATCC® No. Organism Strain Type Strain Sequenced Genome

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ATCC® No. Organism Strain Type Strain Sequenced Genome

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27048D Rhodospirillum rubrum ICPB 2204 27340D-5 Ruminococcus productus VPI 4299 (2396) Y BAA-605D Salinibacter ruber DSM 13855 Y YBAA-731D Salmonella enterica subsp. arizonae BAA-639D Salmonella enterica subsp. Diarizonae 49284D Salmonella enterica subsp. enterica serovar Minnesota mR595 9150D-5 Salmonella enterica subsp. enterica serovar Paratyphi A YBAA-1250D-5 Salmonella enterica serovar Paratyphi B SPB7 700931D-5* Salmonella enterica subsp. enterica serovar Typhi Ty2 Y700720D-5 Salmonella enterica subsp. enterica serovar Typhimurium LT2 Y27137D-5 Serratia marcescens CDC 3100-71 700550D Shewanella oneidensis MR-1 Y Y29903D-5 Shigella flexneri 24570 Y 700930D-5 Shigella flexneri 2457T Y700808D Silicibacter pomeroyi DSS-3 Y YBAA-590D Silicibacter pomeroyi DSS-10 27556D Spiroplasma citri Morocco-R8-A2 Y 10832D-5 Staphylococcus aureus subsp. aureus Wood 46 35556D-5 Staphylococcus aureus subsp. aureus SA113 700699D-5 Staphylococcus aureus subsp. aureus Mu50 Y12228D-5 Staphylococcus epidermidis FDA strain PCI 1200 Y35984D-5 Staphylococcus epidermidis RP62A Y29970D-5 Staphylococcus haemolyticus SM 131 Y 700236D Staphylococcus hominis subsp. novobiosepticus R22 Y 43809D Staphylococcus lugdunensis N860297 Y 15305D Staphylococcus saprophyticus Y Y43808D-5 Staphylococcus schleiferi subsp. schleiferi N850274 Y 13637D Stenotrophomonas maltophilia 810-2 Y BAA-611D-5 Streptococcus agalactiae 2603 V/R Y9809D Streptococcus gallolyticus 38 49456D-5 Streptococcus mitis NCTC 12261 Y 25175D Streptococcus mutans NCTC 10449 Y 700610D-5 Streptococcus mutans UA159 Y6308D-5 Streptococcus pneumoniae 6314D Streptococcus pneumoniae BAA-255D-5 Streptococcus pneumoniae R6 YBAA-334D-5 Streptococcus pneumoniae TIGR4 Y700294D-5 Streptococcus pyogenes M1 GAS, SF370 Y12344D-5 Streptococcus pyogenes typing strain T1 Y 9759D Streptococcus salivarius B2 BAA-1024D Streptococcus salivarius DSM 13084 10556D-5 Streptococcus sanguinis Y BAA-853D Streptococcus suis P1/7 BAA-250D Streptococcus thermophilus LMG 18311 Y31267D-5 Streptomyces avermitilis NRRL 8165 Y Y27419D-5 Streptomyces avidinii ISP 5526 Y 35852D Streptomyces cattleya NRRL 8057 23915D Streptomyces griseinus ISP 5047 Y 69445D Streptomyces lividans FD 29419 BAA-471D-5 Streptomyces violaceoruber M145 YBAA-1157D Sulfitobacter sp. BAA-1142D Sulfitobacter sp. EE-36 35092D-5 Sulfolobus solfataricus DSM 1617 Y27184D Synechocystis sp. PCC 6803 Y43037D-5 Tannerella forsythensis FDC 338 Y 700265D Thauera aromatica T1 700841D Thermaerobacter marianensis JCM 10246 Y 700654D Thermococcus gorgonarius DSM 10395 Y 700653D Thermococcus pacificus DSM 10394 Y 700529D Thermococcus zilligii DSM 2770 Y

25905D-5 Thermoplasma acidophilum 122-1B2, DSM 1728 Y Y51530D-5 Thermoplasma volcanium DSM 4299, GSS1 Y Y43589D-5 Thermotoga maritima DSM 3109 Y Y700961D Thermus antranikianii HN3-7 Y 700962D Thermus igniterrae RF-4 Y 33923D Thermus thermophilus AT-62 BAA-163D-5 Thermus thermophilus DSM 7039 Y10790D Veillonella parvula Te 3 Y 39315D-5* Vibrio cholerae N16961 Y51394D-5* Vibrio cholerae MO45 700601D Vibrio fischeri ES114 Y17802D-5 Vibrio parahaemolyticus EB101 Y 33313D Weissella paramesenteroides NCDO 803 Y 29543D-5 Wolinella succinogenes DSM 1740, FDC 602W Y Y49118D Xanthomonas axonopodis pv. citri 3213 Y 13902D Xanthomonas axonopodis pv. vasculorum XV 101 33913D Xanthomonas campestris pv. campestris NCPPB 528 Y Y35933D-5* Xanthomonas oryzae pv. oryzae PDDCC 3125 Y 49119D Xanthomonas phaseoli G-27 Y 35881D Xylella fastidiosa 2694 PCE-FG 700964D Xylella fastidiosa Temecula Y9610D Yersinia enterocolitica 33114 Y 13979D-5 Yersinia pseudotuberculosis NCTC 8580 4284D-5 Yersinia pseudotuberculosis 447 * Distributed only within the United States

10895D-5 Ashbya gossypii NRRL Y-1056 Y10231D-5 Candida albicans 3147 14053D-5 Candida albicans NIH 3172 MYA-2876D Candida albicans SC5314 2001D-5 Candida glabrata CBS 138 Y Y36909D Candida glabrata NCYC 388 66028D Candida kefyr AmMS 226 96144D Candida parapsilosis MCO448 66029D Candida tropicalis AmMS 227 66030D Cryptococcus albidus AmMS 228 18803D-5 Cryptococcus laurentii CBS 139 Y 66031D Cryptococcus neoformans AmMS 229 MYA-565D-5 Cryptococcus neoformans JEC 21 Y76484D-5 Cryptococcus neoformans YBC 81 36239D-5 Debaryomyces hansenii CBS 767 Y Y28958D-5 Filobasidiella neoformans NIH 430 8585D-5 Kluyveromyces lactis NRRL Y-1140 Y14522D Malassezia pachydermatis CBS 1879 Y 2601D-5 Saccharomyces cerevisiae NRRL Y-53 9763D-5 Saccharomyces cerevisiae NRRL Y-567 204508D-5 Saccharomyces cerevisiae S288C Y

30010D Acanthamoeba castellanii Neff 50608D Blastocystis hominis BT1 PRA-67D Cryptosporidium parvum Iowa strain Y50984D Perkinsus atlanticus ALG1 50439D Perkinsus marinus CRTW-3HE (MD-1) 30007D Tetrahymena thermophila WH-6 Y 30001D Trichomonas vaginalis C-1:NIHA 30266D Trypanosoma cruzi Tulahuen

Genomic DNA from Protozoa

ATCC® No. Organism Strain Type Strain Sequenced Genome

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Genomic DNA from Fungi and Yeast

The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffmann-La Roche, Inc. Use of the PCR process requires a license.

PicoGreen® is a registered trademark of Invitrogen.

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