DNA Fingerprinting
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Transcript of DNA Fingerprinting
DNA Fingerprinting
Mark Bailey
Vicki L. Burnett
Walker B. Carroll
What is it?
• Series of techniques used to differentiate one subject’s DNA from another
• Compares hypervariable regions, or variable numbers of tandem repeats (VNTRs)
• Synonyms are DNA profiling, DNA typing, DNA fingerprinting
Who developed it, and when?• Sir Alec Jeffreys, University of Leicester, England
in 1985• Identified hypervariable regions in the DNA• Published two key papers describing how those regions
could be used as ‘fingerprints’ for differentiating individuals, and how those fingerprints might be used in the criminal justice system
• First used in England in an immigration case, then to exonerate a suspect in a murder case
Jeffreys AJ, Wilson V, Thein SL (1985) Individual-specific ‘fingerprints’ of human DNA. Nature 316(4):76-79.Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA ‘fingerprints’. Nature 318(6046):577-579.
Technical advances
• Structure of DNA -Watson, Crick, Franklin
• Sequencing of DNA -Sanger
• Amplification of DNA by the polymerase chain reaction (PCR) -Mullis
• Restriction endonucleases to cleave DNA
• Gel electrophoresis to separate DNA
• Dyes or probes to visualize the DNA
What is it used for?
What is it used for?
• Paternity cases, babies in maternity ward• Forensic science to identity remains of dead
bodies as well as live folks• Characterization of genomes, cell lines,
plant lines• Trace transmission of diseases such as TB
and food-borne pathogens• Identify genetic mutations in cancer, other
diseases• Identify lineage of populations
Advantages
• Probability of two people having the same fingerprint is very small
• DNA profile can’t be altered by suspect• DNA remains intact under many harsh
conditions• May be able to obtain information about an
individual from a relative’s DNA• Leads as much to exonerations of wrongly
accused suspects as convictions of guilty ones
Disadvantages
• Fingerprint is a very small part of the genome, uniqueness questioned
• Matches within certain ethnic groups may be much higher than in other populations
• Human error either at the crime scene, the laboratory, or the police station
• Ethical issues pertaining to identification of genetic defects or medical conditions
DNA data banks• All 50 states have rules on collection of
DNA profiles from criminal cases
• National Combined Data Index System in place (CODIS) in searchable database
• Battery of 13 different markers used
• International databanks incorporating similar markers to facilitate identification of criminals across countries
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