VTEC

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• Simple and Specific

• Design your own Primers

• Results in less than one hour

• Ideal in all Research settings

Design your own isothermal molecular assay DNA and RNA Amplification Kits

IVD solutions through partnership

Why choose Mast Isoplex®?

Mast Isoplex utilises the advantages of loop mediated isothermal amplification (LAMP*) to create effective and convenient diagnostic assays. LAMP is a well published and proven alternative gene amplification method, overcoming many of the practical limitations of working with conventional PCR.

RapidMast Isoplex® produces up to 1000 times more nucleic acid than PCR in an extremely short reaction time of 15-60 minutes.

RobustMast Isoplex® is less prone to substance inhibition than PCR. Accordingly, the assay is ideal for use in blood and faecal samples where inhibition1 can cause difficulties.

SimpleMast Isoplex® achieves excellent results without the need for extensive sample extraction and concentration (a requirement for PCR) with selected assays2.

Advantages of Mast Isoplex®

ConvenienceDNA and RNA amplification kits afford a reduction in the time and complexity of assay development whilst dramatically simplifying the Quality Assurance process required for in-house assays.

Isothermal Amplification Simple temperature management at 60-65°C, allows continuous amplification of target gene sequences, removing the restriction of short periods of extension seen in PCR. Equipment requirements are minimal – a hot block and micro-centrifuge is all that is needed to run the assay. Simple Assay DesignPrimers can be designed using free web-based design tools or from commerciallyavailable software packages.

Single Tube Assay Reverse transcription, double looped template formulation, exponential amplification and detection of all reactions simultaneously in a single tube. As a result, the risk of contamination is significantly reduced.

Visit our website - www.mastgrp.com and download PrimerExplorer v4 primer designing software specifically for LAMP

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45 minutes

Boil at 95°C, quick spin and keep on ice

Add sam

ples and primers to R

eaction mix

Reaction

Read results

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Real-time detection

Benefits of LAMP technology

LAMP technology can be applied to a variety of fields, by designing specific primers for targeting anyDNA or RNA sequence, rendering the assay especially effective for detecting target sequences fromviruses3, protozoa4, bacteria and fungi.

Highly Sensitive and SpecificSignificantly higher sensitivity when compared with real-time PCR, without compromising specificity.

Inhibitory substances have significantly less impact with LAMP, than PCR due to the reaction chemistry and isothermal amplification conditions.

Detection Methods

Two different detection dye options are included within all DNA and RNA amplification kits to aid differentiation:

Visual Detection Dye for visual end point reading • Hydroxynaphtholblue(HNB)isametal indicator, which enables the visual detection of the Mg2+ concentration and its decrease by pyrophosphate precipitation during positive LAMPamplification.TheadditionofHNBto the reaction mix, changes the colour from purple to blue in positive samples during DNA or RNA amplification.

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Fluorochrome Dye

• AllowsforautomatedReal-timedetection on cyclers with a FAM setting for labs with higher throughput requirements.

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0 5 10 15 20 25 30

500

1000

1500

2000

2500

Time (min)

Inte

nsity

(mV

)

Positive Sample

Negative Sample

Visual detection

Sam

ple

prep

arat

ion

Detection

15

minutes

45 minutes

Boil at 95°C, quick spin and keep on ice

Add sam

ples and primers to R

eaction mix

Reaction

Read results

dprim

ers t

Real-time detection

Ordering Information

Order Code Product Kit Size

67DNAMP1 Mast Isoplex® DNA Amp 48 Tests

67DNAMP2 Mast Isoplex® DNA Amp 96 Tests

67DNAMP3 Mast Isoplex® DNA Amp 192 Tests

67RNAMP1 Mast Isoplex® RNA Amp 48 Tests

67RNAMP2 Mast Isoplex® RNA Amp 96 Tests

67RNAMP3 Mast Isoplex® RNA Amp 192 Tests

References1. HisatoshiKanekoa,TakashiKawanab,EikoFukushimaa,TatsuoSuzutani.Toleranceof loop-mediatedisothermalamplificationtoaculturemediumandbiologicalsubstances.J.Biochem. Biophys.Methods70(2007

2. HadersdorferJ,NeumüllerM,TreutterD,FisdcherTC(2011).FastandreliabledetectionofPlumpox virusinwoodyhostplantsusingtheBlueLAMPprotocol.AnnApplBiol,159,456.

3. PlutzerJ,KaranisP.(2009).RapididentificationofGiardiaduodenalisbyloop-mediatedisothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods. Parasitol Res. 104(6):1527 4. NotomiT,OkayamaH,MasubuchiH,YonekawaT,WatanabeK,AminoN,HaseT(2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research 28 (12): e63

* Licensed under International Patent application numbers:WO00/28082,WO01/34790,WO01/34838,WO01/83817,WO01/77317,WO02/24902,WO02/103053and corresponding patents owned by Eiken Co., Ltd., Japan in other countries.

v 2.0 CEA 02/13

United KingdomMast Group Ltd.Mast HouseDerby Road, BootleMerseyside L20 1EA

Tel: + 44 (0) 151 933 7277Fax: + 44 (0) 151 944 1332e-mail: sales@mastgrp.com

GermanyMast Diagnostica GmbHFeldstrasse 20DE-23858 Reinfeld

Tel: + 49 (0) 4533 2007 0Fax: + 49 (0) 4533 2007 68e-mail: mast@mast-diagnostica.de

FranceMast Diagnostic115 Rue Jules BarniCS91106 80011 Amiens CEDEX 1

Tél: + 33 (0) 322 80 80 67Fax: + 33 (0) 322 80 99 22e-mail: info@mast-diagnostic.fr

www.mastgrp.com

Additional products from the Mast Isoplex® range

Order Code Product Kit Size

67VTSCK1 Mast Isoplex® VTEC 48 Tests

67VTSCK2 Mast Isoplex® VTEC 96 Tests