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Ž .European Journal of Pharmacology 346 1998 175–180
Differential effects of 5-HT receptor antagonists in dorsal and1Br1D
median raphe innervated brain regions
Claire Roberts a,), Ana Belenguer b, Derek N. Middlemiss a, Carol Routledge a
a SmithKline Beecham Pharmaceuticals, Department of Neuroscience, New Frontiers Science Park, Third AÕenue, Harlow, Essex, CM19 5AW, UKb SmithKline Beecham Pharmaceuticals, Department of Separation Science, New Frontiers Science Park, Third AÕenue, Harlow, Essex, CM19 5AW, UK
Received 22 September 1997; revised 12 January 1998; accepted 16 January 1998
Abstract
Ž X � X X wŽ .The effect of SB-224289 2,3,6,7-tetrahydro-1 -methyl-5- 2 -methyl-4 - 5-methyl-1,2,4-oxadiazole-3-yl biphenyl-4-x 4 w x X . Ž . Ž wyl carbonyl Furo 2,3-F -indole-3-spiro-4 -piperidine oxalate 4 mgrkg i.p., 5-HT receptor antagonist , GR 127935 N- 4-methoxy-3-1B
Ž . x X X Ž .w X x . Ž4-methyl-1-piperizinyl phenyl -2 -methyl-4 - 5-methyl-1,2,4-oxadiazole-3-yl 1,1 -biphenyl -carboxamide 0.3 mgrkg i.p., 5-HT1Br1D. Ž . Ž .receptor antagonist , and paroxetine 10 mgrkg p.o. were investigated on extracellular 5-hydroxytryptamine 5-HT levels in the frontal
Žcortex, striatum and dentate gyrus of the freely moving guinea-pig with microdialysis. In the frontal cortex and striatum dorsal raphe.innervated areas , GR 127935 evoked a significant decrease in extracellular 5-HT, reaching minima of 41"12% and 32"6% of basal,
respectively. This decrease may be explained by antagonism of inhibitory 5-HT receptors on raphe cell bodies, leading to a local1Br1D
increase in 5-HT, which, in turn, stimulated 5-HT receptors to decrease cell firing, and hence 5-HT release from terminals. In contrast,1AŽ .SB-224289 had no effect on 5-HT levels in either region. In the dentate gyrus median raphe innervated area , GR 127935 and
SB-224289 significantly increased extracellular 5-HT, reaching maxima of 146"11% and 151"19% of basal, respectively. The abilityof both compounds to increase 5-HT levels in the dentate gyrus suggests a lack of 5-HT receptors in the median raphe nucleus.1Br1D
Paroxetine produced a small but non-significant increase in extracellular 5-HT in the frontal cortex, and a small decrease in the dentategyrus. The lack of effect of paroxetine in terminal areas may be due to the limiting effects of cell body 5-HT autoreceptors. In summary,the above data demonstrate that 5-HT receptor antagonists increase 5-HT levels in the dentate gyrus, implying that acute1Br1D
administration of 5-HT receptor antagonists will achieve a similar effect to chronic selective serotonin re-uptake inhibitor treatment1Br1D
in median raphe innervated areas. This, in turn, suggests that such compounds may be efficacious in the treatment of depression. q 1998Elsevier Science B.V.
Ž .Keywords: Microdialysis; Guinea pig ; 5-HT receptor antagonist1Br1D
1. Introduction
Ž .5-Hydroxytryptamine 5-HT release is subject to regu-lation by a negative feedback system mediated by autore-ceptors located on both neuronal terminals and cell bodies.It has been established that the majority of terminal autore-
Žceptors are of the 5-HT receptor subtype Engel et al.,1B
1986; Middlemiss et al., 1988; Hoyer and Middlemiss,1988; Roberts et al., 1994b, 1996; Buhlen et al., 1996;
.Davidson and Stamford, 1996; Hartig et al., 1996 , whilesomatodendritic autoreceptors encompass both 5-HT and1A
) Corresponding author. Tel.: q44-01279-622684; fax: q44-01279-622230; e-mail: [email protected]@inet
Ž5-HT receptor subtypes Starkey and Skingle, 1994;1D.Davidson and Stamford, 1995; Pineyro et al., 1996 . How-
ever, there is some preliminary evidence for the existenceof a small population of 5-HT receptors at 5-HT termi-1D
Žnal locations Limberger et al., 1991; Davidson and Stam-. Žford, 1996 and 5-HT receptors in the raphe Davidson1B
.and Stamford, 1995 .We have previously reported that systemic administra-
tion of both the mixed 5-HT receptor antagonist,1Br1DŽ w Ž .GR 127935 N- 4-methoxy-3- 4-methyl-1-piperizinyl
x X X Ž .w Xphenyl -2 -methyl-4 - 5-methyl-1,2,4-oxadiazole-3-yl 1,1-x .biphenyl -carboxamide , and the selective 5-HT receptor1B
Ž X � Xantagonist,SB-224289 2,3,6,7-tetrahydro-1-methyl-5- 2 -X wŽ .m e th y l-4 - 5 -m e th y l-1 ,2 ,4 -o x a d ia z o le -3 -y l
x 4 w x Xbiphenyl-4-yl carbonyl Furo 2,3-F -indole-3-spiro-4 -
0014-2999r98r$19.00 q 1998 Elsevier Science B.V. All rights reserved.Ž .PII S0014-2999 98 00061-2
( )C. Roberts et al.rEuropean Journal of Pharmacology 346 1998 175–180176
.piperidine oxalate were unable to illicit an increase inextracellular 5-HT in the frontal cortex of the guinea-pigŽ .Roberts et al., 1994a, 1997a,b . One should note thatalthough GR 127935 has been reported to demonstrate
Žpartial agonism in recombinant cell lines Pauwels and.Colpaert, 1995; Watson et al., 1995 , it failed to show
Žintrinsic activity at native terminal autoreceptors Roberts.et al., 1994b, 1996 . Some of these results were confirmed
by another group investigating in vivo 5-HT levels in theŽ .frontal cortex Skingle et al., 1994, 1995 . However, other
groups have demonstrated 5-HT receptor antagonists1Br1DŽto have no effect in the substantia nigra Moret and Briley,
.1995a,b , but to increase 5-HT release in regions such asŽ .the hypothalamus Briley M., personal communication
Ž .and the dentate gyrus Pullar et al., 1996 . These datasuggest that there are region-specific actions of 5-HT1Br1D
receptor antagonists.It is known that 5-HT projections arise from two main
mid-brain nuclei, the dorsal and median raphe nuclei.Although many terminal regions receive projections fromboth nuclei, some contain a majority from one type. Forexample, the frontal cortex has been shown to contain amajority of dorsal raphe projections; the dentate gyrus and
Žhypothalamus, a majority of median raphe projections seeKosofsky and Molliver, 1987 for serotonergic morphol-
.ogy . Therefore, it is possible that dorsal and median rapheprojections demonstrate different pharmacology. There issome evidence in the literature that suggests differencesbetween responsiveness of 5-HT and 5-HT receptor1A 1B
ligands and selective serotonin re-uptake inhibitors in areasinnervated from either the dorsal or median raphe nucleusŽ .Blier et al., 1990a . Blier and colleagues reported that inmedian raphe innervated areas, 5-HT receptors play a1B
more important role in the control of terminal 5-HT re-lease. In contrast, in dorsal raphe innervated areas, 5-HT1A
receptor control is more prevalent.Therefore, the aim of this work was to study multiple
Ž .brain regions to assess effects of: i the selective 5-HT1B
receptor antagonist, SB-224289, and the non-selective 5-Ž .HT receptor antagonist, GR 127935; and ii systemic1Br1D
paroxetine on extracellular 5-HT levels, to compare thecontrol on 5-HT release exerted by 5-HT and 5-HT1B 1D
receptor antagonism in different brain regions. Frontalcortex and striatum were used as example areas receiving5-HT projections from the dorsal raphe nucleus and den-tate gyrus as an example of median raphe nucleus projec-tions.
2. Materials and methods
2.1. In ÕiÕo microdialysis
Ž .Male Dunkin Hartley guinea-pigs Porcellus weighingbetween 300–400 g were used in all experiments. Animalswere maintained on a 12 h light–dark cycle at 228C, and
given free access to food and water. Guinea-pigs wereŽanaesthetised with 5% isoflurane delivered with O 32
.lrmin in an induction chamber. On attaining surgicalanaesthesia, the guinea-pigs were transferred to a stereo-
Ž .taxic frame David Kopf , which had been adapted toaccommodate an anaesthetic mask and scavenging unitŽ .Klapwyk et al., 1995 . Anaesthesia was maintained on2–3% isoflurane with 2 lrmin O :2 lrmin N O.2 2
Brain microdialysis probes were constructed as previ-Ž .ously described in Roberts et al. 1997a . Dialysis probes
Ž . Žwere implanted either into: i frontal cortex AP q4.5. Ž .mm, ML "2.0 mm, 3.0 mm vertical from the dura ; ii
Ždentate gyrus AP y4.1 mm, ML "2.1 mm, 4.5 mm. Ž . Žvertical from the dura , or iii striatum AP q3.0 mm,
.ML "3.3 mm, 7.0 mm vertical from the dura fromBregma.
Probes were secured with two skull screws and dentalacrylic cement, and the wound sealed. Animals were al-lowed 24 h for recovery, after which the probes were
Žperfused with artificial cerebrospinal fluid aCSF; 125 mMNaCl; 2.5 mM KCl; 1.18 mM MgCl ; 1.26 mM CaCl ;2 2
.pH 7.4 at a rate of 2 mlrmin. After 2 h perfusion,samples were collected every 20 min into 10 ml of aCSF.3 samples were taken to measure basal extracellular levelsof 5-HT before drug treatment. Following drug treatments,5-HT levels were measured for 9 further samples. Guinea-
Ž .pigs were injected intraperitoneally i.p. with vehicle orŽdrug after the third dialysis sample i.e., 60 min from the
.start of the experiment . Animals were not re-used.
2.2. High-performance liquid chromatography separation( )with electrochemical detection HPLC-ECD of 5-HT
Dialysis samples of 45 ml were injected onto an HPLCsystem using centre loop filling, and 5-HT separated fromother substances using reverse phase, ion pair chromatog-raphy. Separation was achieved at a flow rate of 0.35mlrmin with a 3 mm=20 cm ODS2, 3 mm columnŽ .Spherisorb , and a mobile phase consisting of 96% bufferŽ0.15 M NaH PO ; 0.3 mM sodium octanylsulphate; 0.12 4
.mM EDTA; pH 3.0 and 4% isopropanol. The mobilephase was filtered through a 0.22 mm GS filter anddegassed with helium.
Detection of 5-HT was performed with an Antec elec-trochemical detector, with a glassy carbon working elec-trode set at q0.65 V vs. a AgrAgCl reference electrode.The detection of 5-HT was linear over the range 2–2000fmol with a limit of detection of 2 fmolrsample underthese conditions.
2.3. Data analysis
Data from experiments were reported as area underchromatogram peaks. The first 3 samples were averaged toyield a basal level of extracellular 5-HT. All samples wereexpressed as percent of basal levels. Percent of basal
( )C. Roberts et al.rEuropean Journal of Pharmacology 346 1998 175–180 177
values for the individual time points post-treatment wereaccumulated and averaged, producing a value for the mean% of basal, which was an estimate of the mean area under
Ž .the curve AUC . Statistical comparisons of mean AUCfollowing drug administration were calculated on a SAS-
Ž Ž .Research Scientist Application v1.4, release 6.08 1992.SAS Institute, Cary, NC 27513, USA . Analyses were
Ž .performed using a one-way analysis of variance ANOVAfollowed by a post-hoc least significant difference t-test.Significance was taken at the 5% level.
2.4. Materials
Ž .Paroxetine, SB-224289 Roberts et al., 1997a and GRŽ .127935 Skingle et al., 1993 were synthesised at SmithK-
line Beecham.Ž .The vehicle used was methyl cellulose 1% . The dose
of SB-224289 was derived from a behavioural animalmodel of 5-HT receptor function, i.e., antagonism of1B
Ž .SKF 99101-induced hypothermia Hatcher et al., 1995 ,and was equivalent to 2=ED . The dose of GR 12793550
was taken from the maximum dose shown to inhibit5-HT receptor agonist-induced contralateral turning1Br1DŽ .Skingle et al., 1993 . This dose of GR 127935 wasequivalent to 0.6=ED derived from the agonist-induced50
hypothermia model.
3. Results
Ž .Basal levels of extracellular 5-HT were 16"3 ns16 ,Ž . Ž .5"1 ns10 and 9"2 ns11 fmolrsample in the
frontal cortex, striatum and dentate gyrus, respectively.ŽIn the frontal cortex, GR 127935 0.3 mgrkg i.p.,
. Ž .ns6 produced a significant decrease P-0.05 in extra-cellular 5-HT, reaching a minimum of 41"12% of basal 3
Žh post-treatment. However, SB-224289 4 mgrkg i.p.,
Ž . Ž . Ž . ŽFig. 1. Effect of ` vehicle ns6 , B GR 127935 0.3 mgrkg i.p.,. Ž . Ž .ns6 and v SB-224289 4 mgrkg i.p., ns4 on extracellular 5-HT
in the frontal cortex. Arrow denotes administration of compounds.
Ž . Ž . Ž . ŽFig. 2. Effect of ` vehicle ns5 and ' paroxetine 10 mgrkg i.p.,.ns5 on extracellular 5-HT in the frontal cortex. Arrow denotes adminis-
tration of compounds.
. Ž .ns4 had no significant effect Fig. 1 . The mean AUCŽ .for both compounds were 58"9% ns6 and 108"6%
Ž . Ž .ns4 , respectively. Paroxetine 10 mgrkg p.o. had nosignificant effect on cortical extracellular 5-HT, with mean
Ž .AUC of 103"9% ns5, Fig. 2 .Ž .In the striatum, GR 127935 0.3 mgrkg i.p., ns3
Ž .produced a significant decrease P-0.05 in extracellular5-HT, reaching a minimum of 32"6% of basal 3 h
Žpost-treatment. In contrast, SB-224289 4 mgrkg i.p.,. Ž .ns4 had no significant effect on 5-HT levels Fig. 3 .
Ž .The mean AUC for both compounds were 57"4% ns3Ž .and 97"3% ns4 , respectively.
ŽIn the dentate gyrus, both GR 127935 0.3 mgrkg i.p.,. Ž .ns4 and SB-224289 4 mgrkg i.p., ns4 significantly
Ž .increased extracellular 5-HT Fig. 4, P-0.05 . GR 127935reached a maximum of 146"11% after 1 h and SB-224289reached a maximum of 151"19% after 2 h drug treat-ment. The mean AUC for both compounds were 129"16%
Ž . Ž . Ž . ŽFig. 3. Effect of ` vehicle ns3 , B GR 127935 0.3 mgrkg i.p.,. Ž . Ž .ns3 and v SB-224289 4 mgrkg i.p., ns4 on extracellular 5-HT
in the striatum. Arrow denotes administration of compounds.
( )C. Roberts et al.rEuropean Journal of Pharmacology 346 1998 175–180178
Ž . Ž . Ž . Ž . ŽFig. 4. A Effect of ` vehicle ns3 , B GR 127935 0.3 mgrkg. Ž . Ž . Ž .i.p., ns4 and B v SB-224289 4 mgrkg i.p., ns4 on extracellu-
lar 5-HT in the dentate gyrus. Arrow denotes administration of com-pounds.
Ž . Ž .ns4 and 129"6% ns4 , respectively. ParoxetineŽ .10 mgrkg p.o. tended to decrease extracellular 5-HT, but
Ž .this did not reach significance Ps0.08 , with mean AUCŽ .of 66"7% ns3, Fig. 5 .
Ž . Ž . Ž . ŽFig. 5. Effect of ` vehicle ns5 and ' paroxetine 10 mgrkg p.o.,.ns3 on extracellular 5-HT in the dentate gyrus. Arrow denotes adminis-
tration of compounds.
4. Discussion
Basal levels of extracellular 5-HT were higher in theguinea-pig frontal cortex than both the striatum and thedentate gyrus. However, 5-HT levels were not predictiveof drug effects, i.e., compounds possessed different actionsin the striatum and dentate gyrus although 5-HT levelswere comparable in these areas.
Regarding the effects of 5-HT receptor antagonists1Br1DŽon 5-HT levels: we have previously reported Roberts et
.al., 1997b that 5-HT receptor antagonists, when1Br1D
perfused directly into a terminal region, in this case thefrontal cortex, were able to increase extracellular 5-HT
Ž .levels, confirming the work of Skingle et al. 1994, 1995 .These studies were interpreted to demonstrate that 5-HT receptor antagonism resulted in blockade of an1Br1D
inhibitory 5-HT tone in the terminal area. These in vivow3 xstudies also confirmed results from in vitro H 5-HT
Ž .release assays Roberts et al., 1996 , where 5-HT1Br1D
receptor antagonists were shown to potentiate release ofw3 xH 5-HT. Therefore, one would predict that systemicadministration of 5-HT receptor antagonists should1Br1D
increase 5-HT release. However, the data obtained in thesestudies suggest that 5-HT receptor autoregulation is1Br1D
much more complex than initially proposed. The effects of5-HT vs. 5-HT receptor antagonists in the different1B 1Br1D
brain regions will be discussed in turn.
4.1. GR 127935-induced decrease in extracellular 5-HT
Differential effects of non-selective versus selectiveligands on 5-HT levels were observed in the dorsal rapheinnervated brain regions, i.e., the frontal cortex and stria-tum. The selective 5-HT receptor antagonist, SB-224289,1B
had no effect on 5-HT, while the mixed 5-HT recep-1Br1D
tor antagonist, GR 127935, decreased 5-HT levels. Thedecrease may be explained by antagonism of inhibitory5-HT receptors on raphe cell bodies that leads to a1Br1D
local increase in 5-HT. This increase in 5-HT in turnstimulates raphe 5-HT receptors to decrease cell firing,1A
hence 5-HT release from terminals. Evidence to supportŽthis theory was demonstrated in a recent paper Roberts et
.al., 1997b , when the GR 127935-induced decrease in5-HT observed in the frontal cortex was attenuated onco-administration of a 5-HT receptor antagonist, WAY1A
100635.
4.2. Lack of effect of SB-224289
Extrapolating from the above hypothesis, one wouldpredict that 5-HT receptor antagonism would also result1B
in an increase of 5-HT in the region of the dorsal raphenucleus, and act on 5-HT receptors to decrease cell1A
firing and terminal 5-HT release. The reason why a de-crease was not apparent in this brain area, under the
( )C. Roberts et al.rEuropean Journal of Pharmacology 346 1998 175–180 179
conditions used, may be explained by either of the follow-ing.
Ž .i The amount of raphe 5-HT generated by cell body5-HT antagonism in the dorsal raphe nucleus is not as1B
large as when both cell body 5-HT and 5-HT receptors1B 1D
are blocked. Therefore, there is a smaller degree of inhibi-tion, via somatodendritic 5-HT receptors, imparted on1A
terminal 5-HT release.Ž . Ž .ii Ceci et al. 1994 have eloquently demonstrated that
8-OH-DPAT inhibits dorsal raphe firing post-synapticallythrough fronto-cortical neurones that project onto dorsalraphe 5-HT neurones. Thus, in a similar way, it may bepossible that there is an alternative projection from the5-HT receptors of the dorsal raphe nucleus to the frontal1D
cortex, mediating inhibition of terminal 5-HT release.
4.3. GR 127935 and SB-224289-induced increase in den-tate gyrus 5-HT
In the median raphe innervated region, both compoundshad a similar effect. The ability of both compounds toincrease 5-HT levels in the dentate gyrus implies thefollowing.
Ž .i In this region, the limiting effects described above,i.e., from raphe cell body 5-HT and 5-HT receptors,1B 1D
may be absent. That is, 5-HT receptors may not be1Br1D
present in the median raphe nucleus.Ž .ii Endogenous 5-HT tone may differ between areas
innervated by neurones projecting from the dorsal com-pared with the median raphe nucleus. If this were the case,then these data suggest that there is less endogenous tonein dorsal raphe innervated areas, as an increase in 5-HTlevels following drug treatment was not apparent in thefrontal cortex or striatum.
Ž .iii There exist pharmacologically distinct terminal au-toreceptors in the two defined regions. It has been sug-
w3 xgested, using the technique of in vitro H 5-HT release,that multiple terminal autoreceptors exist in regions such
Ž .as the hypothalamus Moret and Briley, 1986 , hippocam-Ž . Žpus Wilkinson and Middlemiss, 1992 and cortex Price et
.al., 1993; Roberts et al., 1996 . These areas receive amixture of median and dorsal raphe innervation. Therefore,it is possible that pharmacologically different autoreceptorsexist in brain areas innervated by dorsal rather than medianraphe projections. In support of this, multiple ‘5-HT -like’1D
binding sites have been demonstrated in frontal cortex, butŽ .not striatum Mahle et al., 1991 .
4.4. Effect of paroxetine
As well as potential differences between autoreceptorŽ .function, Blier et al. 1990a have also alluded to differ-
ences in the effects of selective serotonin re-uptake in-hibitors between brain areas in the rat. In contrast, this was
Ž .not replicated by Sharp et al. 1994 . In the present study,acutely administered paroxetine had a similar profile in
both frontal cortex and dentate gyrus. These data agreewith those in other studies, where the lack of effect ofacutely administered paroxetine in these regions has beenattributed to the limiting effects of cell body 5-HT1A
Žreceptors Arborelius et al., 1995; Hjorth, 1993; Invernizzi.et al., 1996 . Therefore, the similar action of paroxetine in
both the dorsal and median raphe innervated areas suggeststhat the density of 5-HT andror 5-HT re-uptake sites in1A
median vs. dorsal raphe are comparable in the guinea-pig.
4.5. Summary
We have demonstrated that in an area we have hypothe-sised to be devoid of 5-HT receptor modulation, both1Br1D
selective 5-HT and mixed 5-HT receptor antago-1B 1Br1D
nists increased 5-HT release in vivo. This is the firstdemonstration that systemic administration of selective5-HT receptor antagonists increase 5-HT in some1Br1D
terminal regions. Increases in 5-HT levels have also beenreported to be elicited by chronic administration of selec-
Ž .tive serotonin re-uptake inhibitors Gardier et al., 1996 .Selective serotonin re-uptake inhibitors are widely used aseffective antidepressants, but have a delayed onset of
Ž .action Montgomery et al., 1994 . The beneficial effect ofselective serotonin re-uptake inhibitors has been attributedto their ability to increase 5-HT following desensitisationof raphe 5-HT andror terminal 5-HT receptors, and it1A 1B
has been suggested that this desensitisation is responsibleŽ .for their delayed onset of action Blier et al., 1990b .
These data therefore suggest that 5-HT receptor an-1Br1D
tagonists will achieve an effect similar to selective sero-tonin re-uptake inhibitors, but have an immediate onset ofaction in median raphe innervated areas. However, effi-cacy as an antidepressant may be limited by a decrease in5-HT release in dorsal raphe innervated areas. This possi-bility may be minimised by using a selective 5-HT1B
rather than a mixed 5-HT receptor antagonist. Valida-1Br1D
tion of this hypothesis now awaits clinical studies with5-HT receptor antagonists.1B
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