Different Stimuli on TailfanAnam Syed, Alyssa Huenniger, Pratiksha Malbari

Materials (Salt: NaCl)

--NaCl solutions

--Syringe with wiring to help direct control and test stimuli

--Crayfish tanks (6)

--Crayfish (6)

--Water (filled halfway)

--Control (Tank Water)

Methods (Salt: NaCl)

--Place crayfish in fresh tank of water

--Begin testing crayfish for reaction

--Insert 3 ml of Tank Water into syringe for control test

--Spray different areas of tail and tailfan/ gills on crayfish; note reaction

--Insert 3 ml NaCl solution (1%, 2%, 3% conc.) into wire syringe

--Spray the same areas of tail and tailfan/ gills; note reaction

Materials (Salt: CaCl2)

--CaCl2 solutions

--Tank Water Control

--Syringe with wiring to help control and direct stimulus

--Crayfish tanks (6)

--Crayfish (6)

--Water (Tanks filled halfway)

Methods (Salt: CaCl2)

--Place crayfish in fresh tank of water

--Begin testing crayfish for reaction

--Insert 3 ml of Tank Water into syringe for control test

--Spray different areas of tail and tailfan/ gills on crayfish; note reaction

--Insert 3 ml of CaCl2 solution (0.3%, 0.6%, and 1.2% conc.) into wire syringe

--Spray same areas of tail and tailfan/gills; note reaction

Materials (Temperature)

--Wire syringe (3) filled with tank water at various temperatures

--3 temperatures used: 12 , 22 , 32℃ ℃ ℃

--Control Tank Water (22 )℃

Methods (Temperature)

--Place crayfish in fresh tank of water

--Begin testing crayfish for reaction

--Insert 3 ml of Tank Water into syringe for control test

--Spray different areas of tail and tailfan/ gills on crayfish; note reaction

--Insert 3 ml of different temperature water (12 degrees Celsius, 22 degrees Celsius and 32 degrees Celsius) into wire syringe

--Spray same areas of tail and tailfan/gills; note reaction

Materials (Mink Urine)

--Mink Urine Solution (1% conc.)

--Tank Water Control

--Syringe with wiring to help control and direct stimulus

--Crayfish tanks (6)

--Crayfish (6)

--Water (Tanks filled halfway)

Methods (Mink Urine)

--Place crayfish in fresh tank of water

--Begin testing crayfish for reaction

--Insert 3 ml of Tank Water into syringe for control test

--Spray different areas of tail and tailfan/ gills on crayfish; note reaction

--Insert 3 ml of 1% concentrated Mink Urine into wire syringe

--Spray same areas of tail and tailfan/gills; note reaction

Materials (Allyl Isothiocyanate)

--Allyl Isothiocyanate (0.1% conc.)

--Tank Water Control

--Syringe with wiring to help control and direct stimulus

--Crayfish tanks (6)

--Crayfish (6)

--Water (Tanks filled halfway)

Methods (Allyl Isothiocyanate)

-Place crayfish in fresh tank of water

--Begin testing crayfish for reaction

--Insert 3 ml of Tank Water into syringe for control test

--Spray different areas of tail and tailfan/ gills on crayfish; note reaction

--Insert 3 ml of 0.1% concentration into wire syringe

--Spray same areas of tail and tailfan/gills; note reaction

--Spray long and short antennae and antennules; note reaction

Results:NaCl

Results:CaCl2

Results:Temperature

Results: Mink Urine

Results: Allyl Isothiocyanate

References

1. Bell, J. C. "The Reactions of Crayfish to Chemical Stimuli." Journal of Comparative Neurology & Psychology (1906).

2. Lung, Megan E., et al. "Effect of increasing salinity levels on the foraging behavior of Orconectes virilis in a controlled environment." (2012).

3. Spoor, W. A. "Loss and gain of heat-tolerance by the crayfish." The Biological Bulletin 108.1 (1955): 77-87.

4. Taylor, Robert C. "Thermal preference and temporal distribution in three crayfish species." Comparative Biochemistry and Physiology Part A: Physiology 77.3 (1984): 513-517.