Diego Padoan DVM - VinfeedENVIRONMENT-technical floor, ventilation, lightness - climate: season,...
Transcript of Diego Padoan DVM - VinfeedENVIRONMENT-technical floor, ventilation, lightness - climate: season,...
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Mikotoksini kod svinja & ImunitetDiego Padoan DVM
Novi Sad, Svinjarstvo 2014
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Plan prezentacije
Ukratko o mikotoksinima
Izloženost kombinaciji mikotoksina
Delovanje na imuni sistem
Efekti na plodnost
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Istraživanje 2014 u Južnoj Evropi
Južna Evropa
Afla [ug/kg] ZEN [ug/kg] DON [ug/kg]
FUM [ug/kg]
OTA [ug/kg]
T-2 [ug/kg]
Broj testiranih uzoraka 286 306 342 231 241 253
Pozitivni uzorci 157 55 171 164 110 27
% Pozitivnih 55% 18% 50% 71% 46% 11%
Negativni uzorci 129 251 171 67 131 226
% Negativnih 45% 82% 50% 29% 54% 89%
Prosek kod pozitivnih (ppb) 6 86 411 1563 23 89
Maksimum (ppb) 104,45 1321,09 9902,59 20260,29 595,28 316,53
Prosek(ppb) 3 15 206 1109 10 10
Srednja vrednost pozitivnih (ppb) 2,63 39,64 206,66 711,705 2,175 57,8
Prva četvrtina pozitivnih (ppb) 1,32 18,35 108,6 402,5025 0,8675 48,34
Treća četvrtina pozitivnih (ppb) 5,8 82,585 387,145 1897,65 6,375 98,99
Žetva 2013
Pojava mikotoksina u životinjskoj hrani je često u vezi sa geografskim poreklom
Južna Evropa
Pozitivni uzorci
Negativni uzorci
Broj
uzoraka
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(Bottalico et al., 1998)
Vrste Fusarium Mikotoksini
F. acuminatum T-2, HT-2, DAS, MAS, MON, NEO
F. crookwellense NIV, FUS, ZON, ZOH
F. culmorum DON, ZON, NIV, FUS, ZOH, AcDON
F. equisetiDAS, ZON, ZOH, NIV, DAcNIV, MAS,FUS
F. graminearumDON, ZON, NIV, FUS, AcDON,DAcDON, DAcNIV
F. heterosporum ZON, ZOH
F. poae DAS, MAS, NIV, FUS, T-2, HT-2, NEO
F. proliferatum FUM, BEA, MON, FUP
F. sambucinum DAS, T-2, NEO, ZON, MAS
F. sporotrichioides T-2, HT-2, NEO, MAS, DAS
F. subglutinans BEA, MON, FUP
Mešavina 11 mikotoksina
Na jednom polju: prosečno od 10 do 15 različitih vrsta Fusarium (Besenhofer, AGES, Vienna)
Zašto smo zabrinuti zbog mešavine toksina ?
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Stvarnost
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“Jačina delovanja jednog mikotoksina se uvećava prisustvom drugih”
Više od jednog?!
Ispod limita
1 mikotoksin
>1 mikotoksin
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O
H O
O H
O
O CH 3
O
O H
H
H
H
C H3
H
O
O
H O
H3
C
H
C H2
O H
Jačina delovanja jednog mikotoksina se uvećava
prisustvom drugih mikotoksina = SINERGIZAM
• T-2 toksin deluje sinergistički sa DON-om (Friend et al., 1992)
• Aflatoksin i T-2 toksin imaju sinergistički efekat (Pierce 1991)
Sinergistički efekti
Zearalenon (ZON) Deoksinivalenol (DON)
1 ZON+1 DON=11
(Trenholm et al., 1988)
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Mikotoksini dođu i prođu
Simptomi povezani samikotoksikozama mogu se pojaviti kada je koncentracija toksina ispod granice detekcije.
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Efekti faktora rizika na preformanse prasadiBob Goodband et. al, J An. Sci 2014
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Interakcija: iskustvo sa prasićimaLesio
n s
core
Interakcija aditivaoštećenje jetre
Interakcija aditiva na specifičan humoralni odgovor IgG protiv ovoalbumina kod prasadi
Kontrola
DON
FB
DON+FB
Antagonistička interakcija na specifičan humoralniOdgovor IgA protiv ovualgumina kod prasadi
Grenier et al. 2011
Arb
itra
ry u
nits
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Interakcija: iskustvo sa prasićimaPro
life
ration index
Interakcion lower than additivein the cell specific answer
Grenier et al. 2011
Expre
ssio
n level
Citokini koji učestvuju u obnavljanju antigenih ćelija
Citokini koji učestvuju u aktiviranju specifičnog imunog odgovora
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Interakcija:specifični imuni odgovor
Citokini uključeni u obnavljanjeantigenih ćelija
Bertrard 2011
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Interakcija:specifični imuni odgovor
Bertrard 2011
Citokini uključeni u aktiviranjespecifične imune reakcije
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Interakcija:specifični imuni odgovor
Bertard 2011
Nesposobnost proliferacije limfocitaposle antigenske stimulacije
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Interakcija: specifični imuni odgovor
Bertrard 2011
Što dovodi do izmenjene proizvodnje specifičnih antitela.
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• DON-glukozid na kukuruzu (Sewald 1992) i pšenici (Berthiller 2004)
• ZON-4-sulfat na pirinču
(Berthiller 2004)
• Hidroksilacija i Glukozilacija OTA u pšeničnim i
kukuruznim kulturama (Ruhland 1994)
• Fumonizini su delimično vezani za proteine u hrani
(npr. kornfleks) (Shier 1990)
•Zearalenon-4-Glukozid
Engelhardt 1988,(e.g. glucosides, glucuronides, fatty acid esters, Savard 1991,
Zearalenone released during digestion (Gareis, 1994)
H
H
HHO
O
CH3
OH
CH3
O
OH
O
OH OH
OH
O
OH
O
CH3
H
O
OOH
OS
O
O
O
DON-3-Glucoside
ZON-4-Sulfate
Maskirani mikotoksini
Ne mogu da se detektuju uobičajenim metodama analize.
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Osetljivost
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T-2, DON, Ergoti
• Smanjen unos hrane
• Lezije na koži i ustima
• Odbijanje hrane
•Povraćanje
• Smanjen rast
T-2, Ergoti
• Lezije na koži i ustima
• Vazokonstrikcija
(nekroza)
T-2, DON, AFB1, OTA, FUM,
Endotoksini
• Krvarenja u crevima
• Oštećenja bubrega
• Bleda i masna jetra
• Plućni edem svinja (PPE)
• Povećana konzumacija vode
• Groznica*
ZON, T-2, DON,
Ergoti
• Neredovno gonjenje
• Abortusi
• Lažna suprasnost
• Niska stopa začeća
• Ciste na jajnicima
• Smrt embriona
• Nekroza repa
• Nimfomanija
• Uvećanje materice
• Smežurano vime/
Agalakcija
• Mrtvorođena prasad
AFB1, T-2, OTA
• Dijareja
• Krv u fecesu i urinu
• Zapaljenje bešike i bubrega
T-2, DON, AFB1, OTA, FUM
• Smanjene performanse
• Imunosupresija
• Nekroza pankreasa
*bolesti u vezi sa endotoksinima
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ZON: hipertrofija uterusaOTA: krv u mokraći
Efekti mikotoksina kod svinja
OTA: Oštećenje bubrega
DON: Kožne lezije
AFB1: masna jetra
otečena vulva, prolapsus
ZON/DON: Splay legs
Otečenavulva
Crvenilo bradavica
ZON
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Aflatoksin
OO
OCH3O
O
O
Hrana za životinje i ljude na koju deluje
• žitarice(kukuruz, ječam, sirak, ovas, pirinač, kineska šećerna trska, pšenica)
• kikiriki, pistaći, badem
• ostalo jezgrasto voće
• soja
• seme pamuka
• mleko, mlečni proizvodi (sir)
• jaja
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Aflatoksin
Aflatoksini su hepatotoksini!
Source: National Animal Disease Center, Ames, USA
Ciljani organ
OO
OCH3O
O
O
Aspergillus flavus, Aspergillus parasiticus
Sindrom blede jetre
= JETRA
Aspergillosis-pneumonia
Rezidue• jetra• mleko (AFM1)
Imunosupresivan, karcinogen, krvarenje
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Vakcinacija kod prasadi
Patogen: Mycoplasma agalactiae (laboratorijski antigen)Mikotoksin: Aflatoksini u hrani 0,14-0,28 mg/Kg
Vakcina Režim Titar specifičnih antitela
Ekspresija citokina
Da kontrolna
Da aflatoksini
Marin et al. 2002
Isto sa fumonizinimaTaranau et al. 2005
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Ohratoksin
Hrana za životinje i ljude na koju deluje
• zrnevlje žitarica(ječam, kukuruz, ovas, pšenica)
• kikiriki
• zrna kafe
• suvi pasulj
• grožđe
• sir
• meso
OH O
H
HH
COOH
Cl
O
CH3N O
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OhratoksinOH O
H
HH
COOH
Cl
O
CH3N O
• Krv u urinu i fecesu
• dijareja
Reziduebubreg, jetra, meso
Akutni simptomi kod prasadi
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Ohratoksin - prasad
• Povećana konzumacija vode• Oštećenje bubrega (“porcine nephropathy”,
renalna disfunkcija)• Oštećenje jetre i bešike• Zapaljenje bešike i bubrega
Hronični simptomi
REZIDUEbubreg, jetra, meso
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Infekcije kod prasadi
Patogen: Salmonella enterica, prirodna pojavaMikotoksin: Ohratoksin u hrani 0,7 mg/Kg
Tretman Poređenje S. enterica
Kontrolna 0/6
OTA 1 ppm 2/6
Ota 3 ppm 6/6
Stoev et al. 2000
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TrihoteceniO
CH2
OHH3C
CH3
H
HHH
H
O
OCH3IOAc
OAc
O
OOO
H3C
Hrana za životinje i ljude na koju deluju
•Zrna žitarica
(ječam, kukuruz, ovas, pšenica, raž, sirak)
• Orasi
• Soja
• Šećerna trska
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Trihoteceni
• Oralne i dermalne lezije
• Poremećaji varenja
(povraćanje DON >2ppm, dijareja)
• Odbijanje hrane (>1.3 ppm DON)
• Nekroze
Akutni simptomi
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Oštećenja kože
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Efekti faktora rizika na performanse prasadi
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Trihoteceni
Hronični simptomi:
• konjuktivitis
Nespecifični primeri:
• Imunosupresija
• Smanjene performanse
Hronični simptomi:
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Konjuktivitis
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DON smanjuje iskoristivost hranljivih materija putem SGLT-1
DON remeti crevnu apsorpciju hranljivih materija, kao što su glukoza i aminokiseline.
Niska koncetracija DON-a inhibira SGLT-1
SGLT-1 je odgovoran za iskoristivost glukoze
SGLT-1 je takođe odgovoran za reapsorpciju vode –> mogući razlog što DON izaziva dijareju
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Poremećena propustljivost creva Bertrand 2010
Mikotoksin: deoksinivalenolIn vitro ogled: linije epitelnih ćelija, ljudskih i svinjskih
Tretman ćelija sa DON-omi merenje TEER = Trans-Epitelna Električna Otpornost
TEER se smanjuje posle Povećane izloženosti DON-u.
Pinton et al. 2008
Pinton et al. 2009
Povećani transepitelijalni prolaz dekstranai bakterija: poremećena propustljivost creva
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Propustljivost creva
Proteins of the junctions:- klaudin 1,2,3,4....- okludin- kaderini- itd.
Porcine cells IPEC-1
Klaudin 3
Klaudin 4
Smanjen intenzitet istaknutosti pri fluorescentnoj mikroskopiji posle izlaganja DON-u: smanjena ekspresija proteina koji utiču na propustljivost ćelijePinton et al. 2009
Control DON
Band inte
nsity (
arb
itra
ry u
nits)
(Bracarense et al. 2012)
Adherencejunction
Tightjunction
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(15 acetil-)DON
Mikotoksin: DONIn vivo ogled, prasad hranjena sa 2,5 mg/Kg
Bracarense et al. 2011
Kontrolna životinja Posle 5 nedeljaSpajanje i izravnavanje resicaIsprekidan epitel
Crevna mukoza posle 5 nedelja uzimanja hrane u kojoj se nalazi DON
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Hrana za životinje i ljude na koju deluju
• Kukuruz (trulo jezgro)
•Ostala zrna žitarica
C
Me
O
O Me OH
OH OH
NH2
C O
COOH
HOOC
O
COOH
HOOC
Fumonizini
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Rezidue
o jetra
o bubreg
Svinje
Akutni simptomi
• PPE1 (edem pluća kod svinja)
Hronični simptomi
• nekroze pankreasa
• oštećenje jetre
• uticaj na imuni sistem
Fumonizini
• Zapaljenja, odbrana: aktivnost makrofaga je smanjena
• Humoralni imunitet: nema poznatih efekata
• Ćeliski imunitet: smanjena proliferacija limfocita i prirodnih ćelija ubica
• Eksperimentalne infekcije: porast smrtnost
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Iskustva sa prasadimaCum
ula
tive c
ough f
requence
Days
(Halloy et. Al. 2005)
Patogen: Pasteurella multocida, intra-trahelna infekcijaMikotoksin : Fumonizin B1, u hrani 5-8 mg/Kg
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Imunomodulacija: Posledice Bertrand 2010
Mikotoksin: fumonizini, u hrani 0,5 mg/Kg lw
E. Coli data oralno 1.1 x 109 CFUs
Povećana kolonizacija bakterija u crevima prasadi
Oswald et al. 2003
Pri ekvivalentnim dozama fumonizina i pri istom načinu davanja E. Coli Devriedent et al. (2009) je pokazao:- smanjena ekspresija citokina- promena antigena u funkciji imunokompetentnih crevnih ćelija
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Ergot –alkaloidi kod prasadi
• Dva glavna znaka bolesti :
- Gangrene ili konvulzije
- Gastrointestinalni poremećaji
• Vazokonstrikcija izaziva negativne efekte na kardiovaskularni i centralni nervnisistem.
• Agalakcija, odbijanje hrane i posledično smanjen prirast .
• Jaki uterotonični efekti, uzrokuju mrtvorođene i smanjenu stopu suprasnosti.
• Ostali znaci
– Proizvodni gubici
– Laminitis
– Nekroze ušiju, repova, i nogu
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Ergot
Krmače inficirane sa sirkom-ergot (17% C. africana ergot sclerotia) pre prašenja,pokazuju:
Smežurano vime ,
Oskudna proizvodnja kolostruma ,
Znaci estrusa,
Mrtva prasad zbog gladi
• Krmače hranjene sa žitaricama posle prašenja pokazuju ozbiljno smanjenjeproizvodnje mleka zbog preniskog nivoa prolaktina.
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Zaključak
Simptomi
Uticaj na performanse(rast, anoreksija)
Oštećenje jetre
Oštećenje bubrega
Reproduktivni problemi(abortus, neplodnost)
Edem pluća
Uticaj na imuni sistem
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Diferencijalna dijagnoza !
?!
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Naturally ahead
PEN No. 28
SUBKLINIČKE mikotoksikoze kod svinja
Naturally ahead
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Naturally ahead
SUBKLINIČKE mikotoksikoze kod svinja
Naturally ahead
PEN No. 26
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Naturally ahead
PEN No. 26 PEN No. 28
SUBKLINIČKE mikotoksikoze kod svinja
DON: 1500 ppbZON: n.d.Init.wgt 8,48 kgWgt. day 42 25,89kgDWG 414 g
DON: n.d.ZON: n.d.Init.wgt 8,48 kgWgt. day 42 27,13 kgDWG 444 g
Naturally ahead
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Naturally ahead
Mali predah
Pauza
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Pitanje plodnosti
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Zearalenon
Hraniva na koja utiče
•kukuruz
• pšenica
• sirak
• ječam
• ovas
• pirinač
Pojavljuje se zajedno sa DON-om!
O
O
OH
HO
O CH 3
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Zearalenon
• Uvećanje mamarnih žlezda
• Otečenost i crvenilo vulve
• Rektalni i vaginalni prolapsusi
Simptomi kod krmača
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Rektalni prolapsus
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Zearalenon
• utiče na cikluse, začeće, ovulaciju, implantaciju
• lažni graviditet
• abortusi
• nimfomanija
• embrionalna uginuća (7.-10. dana)
• inhibicija razvoja fetusa
• smanjen broj fetusa
• hipertrofija (uvećanje) materice
• atrofija jajnika
Krmače: ZON uzrokuje probleme u plodnosti
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Zearalenon
• Povećanje mamarnih žlezda
• Smanjen kvalitet semena
• Atrofija testisa
• Otečen prepucijum
Simptomi kod nerastova
Smanjena spermatogeneza, koju uzrokuje ZON
Feminizacija
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• crvenilo bradavica
• otečenost i crvenilo vulve
• raskrečenost nogu
Simptomi kod prasadi
Zearalenon
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Primer
Nekoliko slučajeva u jednom leglu !!!
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Nedavni slučaj
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ENVIRONMENT
-technical
floor,
ventilation,
lightness
- climate:
season,
temperature,
wind
MANAGEMENT
-animal care
heat detection
instruments,
mating time and way
transport
moving,
cleaning,
mixing,
confinement
- storage/buy
- feeding
way,
ratio,
protein r.
energy r.
vitamin c.
mineral c.
trace mineral c.
poisoning
water supply
DISEASE
- viral
PRRS
parvovirus
circovirus
influenza v.
enterovirus
pestivirus
- bacterial :
E. coli
Salmonella
Streptococcus
Staphylococcus
Pasteurella
Leptospira
Erysipelothrix
Chlamidia
Mycoplasma
Listeria
FERTILITY
MYCOTOXINS
Diferencijalna diagnoza
From signs to affliction
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Plodnost
ZEN
Fitoestrogeni, sve žitarice [soja]
Zagađenje okoline: dioxini, pftalati
[plastifikatori],nonilfenol-bisfenol [boje, deterdženti,
lakovi]
Pesticidi/herbicidi: PCB, metoksiclor, DDT, kepone.
ZEN
Znaci: Crvenilo vulve/bradavica,anestruskasnija polna zrelost kod nazimica
Način delovanja: hipeoestrogeni efekat
Od problema do dokaza
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Negativ.kontrolna
grupa
Toksin grupa
Oglednagrupa
Pozitiv.Kontrol.Grupa
Deoksinivalenol (DON)
[µg/kg] 0 1000 1000 0
Zearalenon(ZON)
[µg/kg] 0 250 250 0
Mycofix® Plus [kg/t] - - 1.5 1.5
48 hibridna praseta, 42 dana
9 kg, podeljeni u 4 eksperimentalne grupe
Plan ogleda
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Završna težina
Final Weight (kg)
29,8
26,2
29,129,4
24
25
26
27
28
29
30
31
Control 1ppm DON, 250ppb ZON 1ppm DON, 250ppb
ZON, 1,5kg/ton MPL
Mycofix Plus 1,5kg/ton
P < 0.05
ab
a
b
a
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Povećanje materice zbog kontaminacije ZON-om
Toksin grupa Ogledna grupa (uklj. Deaktivator)
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Otečena VulvaToksin grupa1ppm DON, 250ppb ZON
Normalna VulvaOgledna grupa1ppm DON, 250ppb ZONDeaktivator 1.5 kg/t
Otečenost Vulve uzrokovana ZON
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Ukupan Protein
Total Protein Albumin Globulin (g/l)
79,1
57,6
72,875,2
0
10
20
30
40
50
60
70
80
90
Control 1ppm DON, 250ppb ZON 1ppm DON, 250ppb
ZON, 1,5kg/ton MPL
Mycofix Plus 1,5kg/ton
P < 0.05
a
b
aa
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Titar antitela
Titer of vaccination of Pseudo-Rabies
28 days post-vaccination
7,21
6,19
6,82
7,16
5,6
5,8
6
6,2
6,4
6,6
6,8
7
7,2
7,4
Control 1ppm DON, 250ppb ZON 1ppm DON, 250ppb
ZON, 1,5kg/ton MPL
Mycofix Plus 1,5kg/ton
P < 0.05
aa
ab
b
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Negativan efekat DON-a i ZON-a je
potvrđen. Oni imaju uticaj na proizvodne
parametre i zdravstveno stanje.
Imunosupresivni efekat DON-a i ZON-a je
potvrđen brojem antitela u titru .
Potvrđen je i koristan efekat deaktivatora na
proizvodne parametre.
Zaključak
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Gubitak hranjivih materija
2006, G. Morantes
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NOVOSTI…NOVOSTI…NOVOSTI!
DVA BIOMIN-ova proizvodabiće PRVIautorizovani proizvodi za deaktivaciju mikotoksina u EU!
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BIOMIN
PRVA kompanija koja je podnela dosije podfunkcionalnom grupom 1(m): Mycofix® Secure
PRVA kompanija koja je dobila pozitivnoEFSA-mišljenje za “1(m)-proizvode”
Usledilo je samo 5 dosijea od 2010, dva od togaponovo od BIOMIN-a (BBSH 797, FUMzyme).Ostali podnosioci: Kemin (Toxfin), FIM Biotech
(FIMIX) i Ankerpoort (Clinoptilolite).
Mycofix® Secure: EFSA Journal 2010;10(7):2787BIOMIN® BBSH 797: EFSA Journal 2013;11(5):3203
Toxfin® Dry: EFSA Journal 2013;11(4):3179
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BIOMIN
PRVA kompanija koja je dobila EU pozitivne glasove za “1(m)-proizvode”
PRVA kompanija koja je dobila EU-odobrenje za “1(m)-proizvode”
Sastanak SCFCAH: 19-20 Septembar 2013
Mycofix® Secure: Aflatoksin-vezivanjeBIOMIN® BBSH 797: Trihoteceni biotransformacija
Publikacija gore pomenutih EU propisa (završni korak!) je urađena u oktobru 2013!
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Što može da se uradi protiv mikotoksina?
Prevencija „Dobra poljoprivredna praksa“, uzgoj biljaka, Bt
kukuruz…… tokom procesa proizvodnje hrane
Dekontaminacija fizički/hemijski tretmani
čišćenje, mehaničko sortiranje, zračenje, ekstakcija rastvaračem ; amonijak, natrijum hidroksid, oksidacioni i redukcioni agensi...
… tokom procesa prerade hrane
Deaktivacija putem aditiva za hranu Adsorpcija
Biotransformacija… tokom procesa varenja hrane
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Bioprotekcija
… za eliminaciju toksičnih efekata
Adsorpcija… za eliminaciju toksina
Biotransformacija
… za eliminaciju toksičnosti
Mycofix® – Prirodni napredak uupravljanju rizikom mikotoksina!
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Adsorpcija... za eliminaciju toksina
> 300 materijala je testirano na kapacitet adsorpcije Aflatoxina od strane IFA i Christian Doppler Lab, u saradnji sa BIOMIN-om
Material Br. uzorakaBentoniti 88
Organske gline 10Diatomejska zemlja 7
HSCAS 19Kaolini 7Ugalj 3MOS 13Drugi 150
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Uobičajeni test vezivanja 200 ppb AfB1, 0.2% adsorbensa, pH 3 i
pH 6.5
Testiranje pod“intenziviranim” uslovima
4 ppm AfB1, 0.02% adsorbensa, pH 5
Određivanje indeksa hemisorpcije
Extrakcija sametanolom ihloroformom
Vezivanje hranjivihmaterija (vitamini, mikro-
elementi)
dalje testiranje
dalje testiranje
dalje testiranje
Ocena vezujućih materijala - šema testiranja
Adsorbens pomešansa nutrientima,
analizirano sa i bezadsorbensa
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Efikasnost u gastro-intestinalnom traktu (in
vitro)
Adsorpcija izotermaVisoka AfB1 i niska
koncentracija adsorbensa
In vivo procena
dalje testiranje
dalje testiranje
dalje testiranje
Provera na rizične zagađivače
Studija vezivanja u želučanom soku
Teški metali, dioksini
Ocena vezujućih materijala-šema testiranja
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Adsorpcija... za eliminaciju toksina
Visok kapacitet adsorpcije (µg toksina/mg adsorbenta; 20 µg Aflatoksina/mg Mycofix®-a)
Jako vezivanje → Nema/slaba desorpcija već adsorbovanih toksina
Visok afinitet prema toksinu → Nema/slaba adsorpcija bitnih hranjivih materija
Adsorbenti ne smeju biti toksični → laboratorijski testovi i in vivo ogledi
Rezultati dovode do zaključka da Izbor pravog adsorbenta treba da se bazira na :
Vekiru et al., 2007
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BIOMIN – dozvola na snazi posle zvaničnog objavljivanja
Mycofix® SecureKOMISIJA ZA SPROVOĐENJE PROPISA (EU) Br. 1060/2013 od 29 Oktobra 2013 odnosi se na dozvolu za bentonit kao dodatak hrani za sve životinjske vrste
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Bioprotekcija
… za eliminaciju toksičnih efekata
Adsorpcija
… za eliminaciju toksina
Biotransformacija
… za eliminaciju toksičnosti
Mycofix® – Prirodni napredak u upravljanju rizikom od mikotoksina!
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“Najznačajniji nalaz našeg rada je taj da ZOM-1 metabolit nije estrogenik in vivo i ne reaguje in vitro sa proteinima estrogenih
receptora ”
Biotransformacija… za eliminaciju toksičnosti
OH
OHOH
O
O
OH
O
1
2
3
4 6
5 1'
2'
3'
4'
5'
6'7'
8'
9'10'
11'
12'
Biološki konstituent dobijen od kvasca proizvodi esterazu.
Esteraza cepa laktonski prsten usled hidrolize estarskih grupa.
*Vekiru et al., 2010
Biološki konstituent - ZEN
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Inaktivirani bioprotein proizvodi epoksidazu koja cepaepoksidni prsten trihotecena (npr. DON)
Biotransformacija… za eliminaciju toksičnosti
Inaktivirani bioprotein - Trihoteceni
*EFSA Report, 2009Pregled sredstava za detoksikaciju mikootosina-koji se koriste kao aditivi za stočnu hranu: način delovanja, efikasnost i sigurnost hrane
De-Epoxidase
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BIOMIN – autorizacija na snazi od zvaničnog objavljivanja
Biomin® BBSH 797KOMISIJA ZA SPROVOĐENJE PROPISA (EU) Br. 1016/2013 od 23 Oktobra 2013 odnosi se na dozvolu za korišćenje i pripremu soja mikroorganizma DSM 11798 iz porodice Coriobacteriaceae kao dodatka ishrani svinja
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Bioprotekcija
… za eliminaciju toksičnih efekata
ADSORPCIJA
… za eliminaciju toksina
BIOTRANFORMACIJA
… za eliminaciju toksičnosti
Mycofix® – Prirodni napredak u upravljanju rizikom od mikotoksina!
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Mycofix® proizvodna linija
Adsorpcija
Sinergističkamešavina minerala
Biotransformacija
Biološki konstituent, Inaktiviranibioprotein, Biomin® BBSH797, Biomin® MTV, FUMzyme®
Bioprotekcija
Odabrani ekstraktibiljaka i algi
© Copyright 2013 by Erber AG. Austria. All rights reserved.
Afla, ZEN, Trihoteceni,
OTA, FUM, Ergottoksini,
Endotoksini
Zaštita jetre i podrškaimunom sistemu
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Mycofix Plus 3.E
Doziranje: 0.5 - 1.0 kg/t preventivno1.5 - 2.5 kg/t terapija
Prasad
Krmače
Roditeljska jata živine
Muzne krave (20-50 g/c/d)
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Svinje u tovu
Brojleri
Nosilje
Ne-reproduktivne životinje
Doziranje: 0.5 - 1.0 kg/t preventivno1.5 - 2.5 kg/t terapija
Mycofix Select 3.E
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Hvala na pažnji.
Pitanja?