Diagnostic Microbiology and Immunology
Chapter 31 Diagnostic Microbiology and Immunology Lab Diagnostic Time Lines The Swab Clicker Question: Heres Some, Depending on Specimen
Clicker Question Urine Analysis Dip Sticks
Top: After reaction with metabolites in urine. Bottom:Before reaction with metabolites in urine Test time = 60 sec. Measures: glucose, bilirubin, ketones, sp. gravity, pH, protein, urobilinogen, nitrite, esterases.Top two right are false positives to show the color reaction. Eosin Methylene Blue Agar There are hundreds of Selective and Differential Bacteriological Media
Text goes on describing particular samples:wounds, feces, genital swabs, etc. We will cover those when we get to specific diseases. Urethral Swab: Microscopy + Plating on Thayer-Martin Agar
Oxidase Test Reagent poured onto the agar AFTER the colonies grew up. Gas Pack, Anaerobic Chamber Some Clinical Tests Metabolic Reactions The Classic API-20e
Glucose Phenol Red with Durham tube. TSI: triple sugar iron agar. Citrate Test More Clinical Tests Testing Antibiotic Sensitivity
Kirby-Bauer Zone of Inhibition E-test. MIC: Minimal Inhibitory Concentration Some Antibiotics Interpretation of Size of the Zone of Inhibition Pseudomonas aeruginosa MICs
Each numbered row one antibiotic, highest conc at left, lowest at right. Clicker Question: E test Clicker Question: Working in a BSL-4 Lab The Gen Micro Labs in AHC2 are BSL-1, most our research labs are BSL-2. BSL-3 labs:to contain pathogens that could be infective as an aerosol. BSL-1: lab people wear lab coats, gloves..and usually does not involve pathogens. BSL-2: needs to contain pathogens in hoods, etc.Aerosol generating equipment must be in a biosafety cabinet. Disease Progression: Untreated Typhoid Fever
Antibody Titers:Generally done by serial 1:2 dilutions from a blood sample.Highest dilution that brings about an Ab-Ag reaction. Clicker Question: Disease Progression: Untreated Typhoid Fever
Antibody Titers:Generally done by serial 1:2 dilutions from a blood sample.Highest dilution that brings about an Ab-Ag reaction. Clicker Question Monoclonal Antibodies Precipitin Reaction Precipitin Rxns on a Glass Slide
Clicker Question: Agglutination Reactions Latex Bead Agglutination for S. aureus Fluorescent Antibodies
Clostridium septicum stained with FITC-Ab, C. chauvoel stained with Rhodamine-Ab Direct and Sandwich (Indirect) Yellow-Green cells are CD8 Cells Red-orange cells are CD-4 Cells
B and T Cells Yellow-Green cells are CD8 CellsRed-orange cells are CD-4 Cells Cell Sorting Clicker Question: Enzyme Immuno-Assays EIA Kits for Small Clinics Immunoblots: Detecting Blotted Proteins (after electrophoresis) Nucleic Acid Diagnostics PCR Detecting Mycobacteium tuberculosis
Lanes Molecular Weight Standards Lanes 2-12 from different TB patients sputum Lanes from non-TB patients with lung symptoms. Lane 15 from known Mycobacterium tuberculosisDNA.