Developmental Biology I - lab.anhb.uwa.edu.au · Basic Principles of Development 1- Cell Division...
Transcript of Developmental Biology I - lab.anhb.uwa.edu.au · Basic Principles of Development 1- Cell Division...
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Developmental Biology I(Classical Embryology)
Anke van Eekelen, PhDTelethon Institute for Child Health Research
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Development
Differential cell behaviours(division, differentiation, growth, patterning, movement)
the emergence of organised structures (tissues, organs)
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Basic Principles of Development
1- Cell Division • multicellular organism
2- Pattern Formation • Defining the Axes: Body Plan
• initiating Germ Layer Formation
3- Morphogenesis • Formation of 3 Germ layers
4- Cell Differentiation • Blood, Muscle, Nerves …
5- Growth • maturity
Cleavage
Gastrulation
Organogenesis
Event Principle Outcome
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Mouse Fruit Fly (Drosophila)
Developmental cycles for various species
Differences:
shape & look of embryo
length of dev. cycle
Common aspects:cleavage/gastrulation/organogenesis
Chicken
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CleavageCell division
Cleavage: NO INCREASE in size of the embryo - process of just generating many cellsExtraordinarily QUICK cell division (much more than in tumours) The decrease in cytoplasmic/nuclear ratio is crucial
Cleavage parcels up the newly divided nuclei with increasingly smaller sub-divisions of pre-existing cytoplasm and nuclear material (although DNA content remains the same!).
Differential distribution of cellular molecules (mRNA and proteins)
New cells will “inherit” cytoplasm and nuclei with differing amounts of these molecules.
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Examples of specific cellular distribution patterns of cellular components in fertilized oocytes
Frog oocyte
Drosophila oocyte
Vg-1 mRNA in vegetal cortex(in situ hybridisation)
Bicoid mRNA on anterior pole(in situ hybridisation)
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As cleavage proceeds:
Oocyte Morula Blastula(1 cell) (clump of cells ) (bigger but hollow ball of cells)
- Cell heterogeneity
- Cell division
- Maternal determinants
- Embryonic gene expression
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Localisation of cytoplasmic factors(specific mRNAs and proteins) is used as morphogenetic information
to lay the blueprint for the body plan(body axis formation)
maternal vs zygotic factors
A-P & V-D axis
CleavagePattern formation
Fruit fly
Frog Zebrafish
A PVL
RD
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Body PlanOrganisationof a fruit fly
Dorsal
ventral
posterioranterior
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GastrulationMorphogenesis - formation of 3 germ layers
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GASTRULATION is the re-arrangement of the blastula to form:
Outer ectoderm skin and (central) nervous systemInner mesoderm most of the organs
endoderm linings of digestive and respiratory system
Frog Chicken
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Morphogenesis or Developmental Mechanics:
Cell adhesionCell shape
Cell movementCell proliferation/deathExtracellular materials
Changes in these qualities at the single cell level alter the form of the cell groups, the tissues, organs and the entire embryo.
New shapes are moulded and sculpted, cells alter theirbehaviours.
This is moulding and sculpting, but there is no sculptor; the clay forms itself.
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Gastrulation is dependent on
Organizers or signalling centres
Spemann organizer
Hensen’s node
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In the fertilized oocyte (zygote):
Sperm entry defines location of the organizer!
Cortex rotates with respect to internal cytoplasm (microtubule-driven)
Differentially distributed cytoplasmic molecules of cortex will be shifted with respect to differentially distributed moleculesof internal cytoplasm cortical rotation V-D axis
Nieuwkoop center(as part of Spemann organizer)
Frog
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The Nieuwkoop center cells can induce changes in neighbouring cells…….
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Intracellular induction causes cell shaping & re-arrangementthrough
‘Autonomous’ signals: zygotic transcription factorsor
cell conditioning signals: zygotic secretion factors
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Cleavage ends:cytoplasm: nucleus ratio while cells divide
maternally derived molecules (transcriptional repressors in the oocyte cytoplasm?)
increase zygotic gene expression of autonomous & cellular signalling molecules
Gastrulation starts:induction of the Organizer by zygotic signalling molecules………this signals to neighbouring cells ………...controls organization of the Blastula…………. instigates & orchestrates cell re-arrangements…………….to give a 3 layered, patterned embryo
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Gastrulation processin frog
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Chicken
Cle
avag
eG
astru
latio
n
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The end result of gastrulaton in vertebrates generating:
A tube with 3 basic layers
Ectoderm =outer layerepidermis (skin)neural (CNS & PNS)
Endoderm=inner layerabsorptive cellssecretory cells
Mesoderm=middle layernotochordmuscle skeletonconnectivekidneyblood
Cell Differentiation /Tissue & Organ development
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OrganogenesisCell differentiation
Further induction processes mediate
cell differention-tissue and organ development:
Example 1
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Example 2Limb formation: Apical Ectodermal Ridge
Example 3Brain compartmentalization: isthmus
(defines boundary between mid-and hindbrain)
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Developmental eventsare controlled by differential gene expression,
……..which drives cascades of gene-regulatory events,
……….which define differential cell behaviours,
which underlie major developmental processes
crucial to study gene expression & protein function
Next week: Molecular mechanisms underlying development