Development of a Technical Program in Stem Cell Science
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Development of a TechnicalProgram in Stem Cell Science:
Responding to an Emerging Need
National Science FoundationAdvanced Technological Education
DUE 1104210
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Madison College
• 2012 Centennial Celebration
•Over 140 career paths serving more than 45,000 students, including many of the fastest growing occupations identified by the WDWD.
•12 Campuses (8 in Madison, 4 Regional).
•Greater than 90% placement rate for graduates within 6 months.
•Technical colleges generate nearly $7 billion in economic benefits for Wisconsin each year.
Facilities Expansion
Health Education
Ingenuity Center
Protective Services
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Biotechnology in Wisconsin
• Between 2004 and 2009 bioscience employment in Wisconsin grew by nearly 3% in contrast to the rest ofthe economy that shrunk by > 3%
• The average bioscience worker has earnings that are 64% higher than the earnings of a typical Wisconsin employee
• More than 640 Wisconsin bioscience businesses have created nearly 24,000 private sector jobs with atotal economic impact of close to $7 billion
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•Reported industry revenues in 2005 totaled $974,000 and $36.9 million in 2007. In the US, by 2016, stem cell company revenues are projected to exceed $8.5 billion and $16-20 billion by 2020.
•The state of Wisconsin has invested more than $94 million, in addition to private investments to promote growth in the stem cell and regenerative biology sector.
•California Institute of Regenerative Medicine (CIRM) $3 billion in Grants.
The Bioeconomic impact of Stem Cell Science
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Stem Cells & Regenerative BiologyAt Madison College
• In 2007, Madison College piloted our first human stem cell course.
•In 2011, NSF awarded $851,454 to develop a technical education program in Emerging Stem Cell Technologies.
•40 Advisory Board Members: Stem Cell Industry, UW Madison SCRMC, UW Waisman Center for Developmental Disorders, and Morgridge Institute for Research / Wisconsin Institute for Discovery.
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Development of a Technical Program in StemCell Technologies: Responding to an Emerging Need (NSF DUE 1104210)
OBJECTIVES:
I. Development of a 2-semester certificate programemphasizing workforce training in Stem Cell Technologies.
II. Develop educational materials in stem cell technologies fordissemination at the local, regional, and national levels.(web-based distribution, instructional videos, manuals)
III. Promote the growth of Stem Cell Programs in other Colleges& Universities throughout the nation
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Introduction to human Stem Cell Methods: Basic Culture and Characterization
Course Competencies:Characterization of hESCs• Chromosomal analysis, florescence microscopy
• Media Formulation (Feeder-dependent/xeno-free media)
•Generation of Cell Aggregates (EBs)
hESC H9 DAPI StainFor metaphase chromosomes
hESC H9 Cell Aggregates:Embryoid formation
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Scheduled Meeting time: Lecture: Monday 5:00pm to 5:50pm Laboratory: Monday/Thursday 6:00pm-8:50pm
Target audience: AAS Biotechnology, BS in Biological Sciences at or nearing completion,
Post-Baccalaureate, Graduate Students, Laboratory Staff.
Human Stem Cell Technologies Certificate Program: 4 Courses (8 Credits)
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SEMESTER 1: Introduction to human Stem Cells- Basic Culture and Characterization
Course Competencies:• Stem Cells and Bioethics
• Aseptic techniques and routine maintenance of PSC cell cultures.
• Basic techniques for culturing hES (H9) cells:thawing, plating, feeding, passaging, and cell banking.
Pluripotent Stem Cell colony pre-split (10x)
Pluripotent Stem Cell colony pre-split (2.5x)
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Course Competencies:• hES culture systems: Feeder dependent/independent.
hESC H9 Colony onMEF-feeder layer (20x)
hESC H9 Colony – Feeder independentOn Matrigel (10x)
SEMESTER 1: Introduction to human Stem Cells- Basic Culture and Characterization
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Course Competencies:• hESC (H9) and iPS (iMR90-4) Cell Differentiation
Spontaneous & Directed Differentiation of Adherent Cells and Cell Aggregates
•Immunoflourescence Microscopy & Molecular Analysis ofPSC-derived differentiated cells
SEMESTER 2: Advanced human Stem Cells- Differentiation & Applications
HepatocyteDifferentiation
Neural RosetteDifferentiation
CardiomyocyteDifferentiation
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Advanced Cell Culture Education Suite (ACCES)
•2744 sq.ft. Animal Cell Culture Facility.•Construction completed and fully operational, January 2012.
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Advanced Cell Culture Education Suite (ACCES)
Molecular Wet Lab Cell Culture Imaging & Microscopy
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Neuronal Differentiation
2013 Biopharmaceutical Technical Center Institute
STEM CELL SYMPOSIUM
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Summary : Stem Cell Education
With NSF ATE support, we have developed a technical training program in Human Stem Cells to
address the emerging demands of the global bioeconomy. Programming also includes the
creation of 1-day workshops & a 5-day Short Course
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Stem Cells & Regenerative BiologyIn Madison, Wisconsin
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Thank you!
Supported by the National Science Foundation AdvancedTechnological Education Project Grant DUE 1104210
Awarded to Madison College July 2011
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Contact Information
•Lisa Seidman, Ph.DPI, Biotechnology Program Director(608) [email protected]
•Thomas Tubon, Ph.D.Project Director/Co-PI, NSF Stem Cell Program at Madison College(608)[email protected]
•Jeanette Mowery, Ph.D.Co-PI, [email protected]