Determination of Protein Concentration of Unknowns.

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Determination of Protein Concentration of Unknowns

Transcript of Determination of Protein Concentration of Unknowns.

Page 1: Determination of Protein Concentration of Unknowns.

Determination of Protein Concentration

of Unknowns

Page 2: Determination of Protein Concentration of Unknowns.

The Standard CurveStandard

Curve.

Estimation

Estimation by

drawing a line.

Linear Regressi

on

Y=mx+b

`

Page 3: Determination of Protein Concentration of Unknowns.

My PI

Understanding biological and personality and environmental factors that create vulnerabilities to drug addiction.

Rodent models are used.

Anxiety traits and pathways to drug addiction.

Low and high anxiety animals are used to explore cellular cellular and molecular factors to create a drug vulnerability profile.

S Tiffany Donaldson, PhD

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Impulsivity and Amphetamine.

• No living animals.

• Microstructural analysis of Golgi-Cox stained neurons: - High and low phenotyped animals.

• Measures of: - Dendritic length

- Bifurcation of the primary dendrite.

- Quantify and qualify the spine type(s)