Detection of Amnionless Protein in Kidney Sections
description
Transcript of Detection of Amnionless Protein in Kidney Sections
![Page 1: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/1.jpg)
Detection of Amnionless Protein in
Kidney Sections
AuthorsNatasha TongeDr. Roberta Rivi
![Page 2: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/2.jpg)
Acknowledgements
Dr. Roberta RiviDr. Sat Bhattacharya
Dr. Katia ManovaDr. Betul Altay-Ozer
Harlem Children SocietyElizabeth Lacy Lab
MSKCC
![Page 3: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/3.jpg)
Wax in a Different Context
Tissue sample is embedded in paraffin wax
![Page 4: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/4.jpg)
Wax in a Different Context
Tissue sample embedded in paraffin wax
Sliced with microtome
![Page 5: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/5.jpg)
Wax in a Different Context
Tissue sample embedded in paraffin waxSliced with microtome
Placed in Tissue bath
![Page 6: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/6.jpg)
Wax in a Different Context
Tissue sample embedded in paraffin waxSliced with microtomePlaced in Tissue bath
Mounted on a slide
![Page 7: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/7.jpg)
Wax in a Different Context
Tissue sample embedded in paraffin waxSliced with microtomePlaced in Tissue bathMounted on a slide
Automatically Processed
![Page 8: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/8.jpg)
Immunohistochemistry - a process used to detect proteins in a tissue section.
Antigen - a substance that provokes an immune responseAntibody – the protein complex that binds to antigens
Antibodies in a Different Context
Enzyme - A protein that speeds up a chemical reaction.Substrate - The chemical that induces the chemical reaction
![Page 9: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/9.jpg)
Immunohistochemistry - a process used to detect proteins in a tissue section.Antigen - a substance that provokes an immune responseAntibody – the protein complex that binds to antigens
Antibodies in a Different Context
primary antibody
secondary antibody
substrate
enzyme
Antigen
Enzyme - A protein that speeds up a chemical reaction.Substrate - The chemical that induces the chemical reaction
![Page 10: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/10.jpg)
The Anatomy of the Kidney
Proximal Tubule - close to the glomerulus.Ammnionless - protein located on the proximal tubule.
Cortex - outer region of the kidney. Most likely to find the glomerulus and proximal tubule here.Medula - inner region of the kidney. Lacks ammnionless.
![Page 11: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/11.jpg)
The Kidney in Focus (more or less)
![Page 12: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/12.jpg)
The Kidney in Focus (more or less)
![Page 13: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/13.jpg)
The Past and Future of this Experiment
Tissue samples are sectioned, placed on slides and stained
The staining process, immunohistochemistry, makes use of antibodies to induce a color change
In future experiments the same process will be used topinpoint mutations in abnormal mouse tissue.
To Detect Amnionless in Kidney Sections:
That color change enabled us to see the location of the proximal tubules in the kidney sections.
![Page 14: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/14.jpg)
Bibliography
http://upload.wikimedia.org/wikipedia/en/thumb/b/b0/Kidney_section.jpg/180px-Kidney_section.jpg (altered by Natasha Tonge)http://mskweb3.mskcc.org/MolecularCytology/mccf-final.htm
http://en.wikipedia.org/wiki/Human_hair
![Page 15: Detection of Amnionless Protein in Kidney Sections](https://reader035.fdocuments.net/reader035/viewer/2022062502/56814bf5550346895db8ead9/html5/thumbnails/15.jpg)
Fin