Detect and Identify...Detect and Identify Microplate Readers in vivo Imaging Instruments Tube...

Detect and Identify

Microplate Readersin vivo Imaging InstrumentsTube LuminometersRadio HPLC MonitorsGamma Counters

Bio

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. 07

BERTHOLD TECHNOLOGIES GmbH & Co. KG

P.O. Box 100 16375312 Bad WildbadGermany

Phone: +49 7081 177-0Fax: +49 7081 177-100E-mail: [email protected]: www.Berthold.com/Bio

Company profile

Berthold Technologies is considered a worldwideleader in luminescence measuring technology andhas provided thousands of luminometers for rese-arch and diagnostics. The Centro XS3 luminometer for academic andpharmaceutical research has been developed withan optimized optics for even higher demands onsensitivity. The CentroLIApc is an ideal instrumentfor diagnostic applications and clinical use, whilethe new CentroPRO is an attractive alternative forresearch.

In recent years we have expanded the develop-ment of microplate instruments which support newmodern measuring methods. Our multimode readerMithras demonstrates exceptional performance inBRET technology, which is mainly used in researchof G-protein coupled receptors.HTRF® certified, the Mithras can be used for thescreening and investigation of biological interac-tions such as kinases or second messengers.

In gene expression studies, reporter genes havebecome an invaluable tool. Using our extremelysensitive imaging instruments the NightOWL, andthe new NightSHADE, the gene expression can beexamined in living organisms.

NightOWLTM and indiGOTM are trademarks of BERTHOLD TECHNOLOGIES. DLR®, DLReady logo, Dual-GloTM and Chroma-GloTM are registered trademarks ofPromega. HTRF® is a registered trademark of Cisbio. UPLC® is a registered trademarkof Waters. AlphaScreen® is a registered trademark of PerkinElmer. Windows® is a registered trademark of Microsoft. CALUX® is a registered trademark of BioDetection Systems. To run certain applications on these productsmay require licenses from others. Some instruments are not available in all countries! BERTHOLD TECHNOLOGIES reserves the right to implement technical improvements and/or design changes without prior notice.

Some techniques for generating and/or detecting light in biological subjects arepatented and may require licences from third parties. Users are advised to independently determine for themselves whether their activities infringe any valid patent.

detect and identifydetect and identify

Bio Gesamt 8-Seiter_Bio Gesamt 8-Seiter.qxp 29.07.10 15:15 Seite 1

Instrument Centro Centro XS3 CentroPRO CentroLIApc CentroLIAPlate formats 96 and 384 wells 96 and 384 wells 96 wells 96 wells 96 wellsSensitivity 5 amol ATP / well

2 zmol fireflyluciferase / well

1.5 amol ATP / well1.5 zmol fireflyluciferase / well

20 amol ATP / well7.5 zmol fireflyluciferase / well

0.002 mIU/L TSH 0.002 mIU/L TSH

Injectors up to 31 in reading position

up to 3 external external external

Heating up to 42 °C ambient ambient ambient ambientShaking 3 modes / 3 speeds 3 modes / 3 speeds 1 mode / 3 speeds 1 mode / 3 speeds 3 modes / 3 speedsSoftware & Operation PC based MikroWin ratio

calculation (DLRTM),kinetics, curve fit automation support

PC based ICE Research incl.ratio calculation(Dual-GloTM)

PC based ICE Immunoassay incl.immunoassay datareduction

on-board sofIAimmunoassay datareductionfor LIA & ILMA

Preferred applications • Aequorin-based Calciummonitoring

• Acridiniumester-basedimmunoassay

• cellular luminescence

• reporter gene assay(single and dual)

• ATP determination

• CALUX® Assays

• reporter gene assay(Dual-GloTM)


• immunoassays (LIA): HRP/ALP labelswith luminescent substrates

Mithras LB 940 Multimode ReaderMithras, the extremely versatile multimode readeroffers you all comprehensive technologies used intoday’s research.

AbsorbanceLuminescenceFluorescence (top and bottom)Fluorescence Polarisation (FP)FRETBRET/BRET2

AlphaScreen®

Time-resolved Fluorescence HTRF®

The unit combines fluorescence and luminescen-ce modes with the uncompromised performanceof dedicated instruments. The sensitivity of theluminescence function exceeds even that of dedicated plate luminometers. Up to 4 reagent injectors and temperature controlare the key features for the instrument´s leadership in functional receptor assays based onBRET or Calcium flux monitoring. HTRF® certified,the Mithras can be used for the screening and investigation of biological interactions such askinases or second messengers.

TriStar LB 941 Multimode ReaderThe TriStar LB 941 multimode reader provides youwith outstanding performance and dedicated opti-cal path system as it is known from the Mithras. TriStar offers you thefollowing technologies:

AbsorbanceLuminescenceFluorescenceFRETBRET/BRET2

The instrument can be equipped with up to 4 injectors for fast kinetics and a temperature control for all cellular assays.

Centro / Centro XS3 LB 960 LuminometersThe Centro and Centro XS3 meet all currentlyknown requirements forversatile, robust and sensi-tive microplate luminome-ters. The selected detectorand the proprietary designof the optical system gua-rantee highest efficiency,lowest background andnegligible crosstalk. Centro XS3 has an optimizedoptics for even higher demands on sensitivity.Fulfilling and even exceeding the requirements toqualify as DLReadyTM, the Centro XS3 is the idealinstrument for luciferase reporter gene assays.Three independently controlled injectors withvariable volume give entire freedom in the selec-tion of assay type and assay sequence.

CentroPRO LB 962LuminometerThe CentroPRO microplateluminometer is an attractiveentry-level instrument in research for all glow-type bio-luminescent and chemiluminescent applications. The instrument is controlled via the easy-to-useICE software which includes ratio calculation forreporter gene applications.

detect and identify

Twinkle LB 970FluorometerThe Twinkle LB 970 is based on a dedicated opti-cal system for fluorescence readings from aboveand below a microplate.Various formats from Petridishes to 384 well platesare supported. The Twinkle can beequipped with tempera-ture control to establishideal conditions for all cell based applications.The high quality design is well suited for sensitiveFRET assays.

Artemis K-101HTRF® ReaderArtemis is a dedicated microplate reader specificallydesigned and approved for HTRF® applications. The instrument supports 96 and 384 well microplateformats.Equipped with single photoncounting detection system, ahighly stable Xenon flashlamp, internal reflection optical unit and well optimized filters the Artemis shows enhanced sensitivity. The software is specifically designed forHTRF® measurements.

Stacker LB 931Plate Handling SystemFor medium and higher throughputBerthold Technologies offers theplate handling system Stacker LB 931.A unique feature of the Stacker is the re-stacking operation mode enablinglong-term studies of e.g. reportergene expression.For positive plate identification bar-code readers are available.

Microplate Readers

CentroLIA LB 961 Clinical LuminometerCentroLIA, the stand–alone microplate lumino-meter with integrated soft-ware, is especially designedfor luminescence immuno-assays (LIA). The use ofsoftkeys with an interactivedisplay of their associatedfunction makes the CentroLIAan extremely user-friendly instrument.The Software has been designed with the routineclinical laboratory in mind and offers protocols forall types of immunoassays.

CentroLIApc LB 962Clinical LuminometerThe CentroLIApc is a micro-plate luminometer especiallydedicated to luminescence immunoassays (LIA). The instrument is controlled via the easy-to-usewizard driven ICE software which includes immuno-assay data reduction. The CentroLIApc is an ideal instrument for diagnostic applications and clinical use.


Monochromator Multimode ReaderMithras2 LB 943*

• double monochromators for excitation & emission• all measurement technologies• all microplate formats• up to 4 reagent injectors

This product respectively some of its configurations will not be available for sale in the U.S.A.

detect and identify

w w w . b e r t h o l d . c o m / b i o

detect and identify

w w w . b e r t h o l d . c o m / b i o

Certain configurations of this product are not available for sale in the U.S.A.

Specifications

Detection Devices 2 low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 850 nm ultra-low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 650 nm (HiSens position) photo diode, spectral range 200 – 1000 nm

Excitation Sources halogen lamp Xenon flash lamp laser, 680 nm (Alpha Technology option)

Monochromator f number: 2.7 (high light transmission) variable bandwidth: 6 - 22 nm increment: 1 nm blocking: 10-6

Filters RFID coded Sample/Plate Formats

6 – 1536 well plates Terasaki plates Petri dishes filter membranes

Temperature Control

up to 45 °C plate incubation area

Shaking 3 modes, 3 velocities Scanning up to 10,000 points per well Injection up to 4 JET injectors

variable volume 10 – 100 µL variable speed tips can be chosen to be located in 8 positions

Kinetics Operation minimum resolution 0.05 sec maximum duration up to 7 days

Automation robot integration

Performance

Luminescence <6 amol ATP per well <3 zmol Firefly luciferase per well (equals less than 800 molecules)

Crosstalk 5 x 10-6 Fluorescence <0.3 fmol FITC per well (filter)

<2 fmol FITC per well (monochromator) Absorbance accuracy <2 % deviation (@ 2 OD, 405 nm)

precision <0.6 % CV (@ 2 OD, 405 nm) Time-Resolved Fluor.

<2 amol Europium per well

HTRF® dF high >900 % Fluorescence Polar.

<5 mP SD (1 nM Fluorescein)

AlphaScreen® <12.5 ng standard beads Dynamic range >6 decades (PMT)

4 decades (photo diode) Injectors accuracy better 98 % over entire volume range

precision better 98 % over entire volume range

Miscellaneous

Dimensions 480 x 500 x 550 mm (w x h x d) incl. injectors

Weight 48 kg OS Win XP, Win Vista, Win 7 Regulations CE, UL, CSA Specifications are subject to change without prior notice

Certain configurations of this product are not available for sale in the U.S.A.

Microplate Readers

TriStar² LB 942Multimode Microplate Reader

With TriStar² the multi-technology micro-

plate reader platform has developed into

its second generation setting new stand-

ards in modularity, performance and user-

friendliness.

Offering the reading technologies

▪ Absorbance

▪ Fluorescence

▪ FRET

▪ Luminescence

▪ BRET

TriStar² is a perfect fit for life sciences re-

search covering all important assays cur-

rently conducted in academic research.

The optical system ONE-4-ALL with a new

revolutionary design is the first time a sin-

gle optical system has been employed in a

multi-technology reader that matches the

performance of individual dedicated optical

devices.

Space for reagent vials is placed at the

front of the instrument providing easy ac-

cess and visibility. A removable trough can

be filled with ice to keep the reagents at

lower temperatures. A special reagent

holder keeps small tubes filled with valu-

able reagents safely in place and enables

using up the volume to the last droplet.

The unit is operated via the proven and in-

tuitive ICE software. Single and multiple

endpoint readings are possible as well as

kinetic and scanning measurements.

The data are displayed in numerical and

graphical formats and may be exported

into EXCEL or printed.

Thus, TriStar² microplate reader offers a

multitude of possibilities and a wide range

of applications. Measurements of enzyme

activities, phagocytosis, Calcium flux, cell

viability, apoptosis, immuno assays, pro-

tein and DNA concentrations and protein-

protein interactions are only a few of the

many uses of TriStar².

Microplate Readers

Apollo 11 LB 913Microplate Absorbance Reader

Based on the proven technology of its pre-

decessors the Apollo 11 sets new targets

for microplate absorbance readers by intro-

ducing its new unlimited life-time LED light

source technology.

The Apollo 11 is ideally suited for all com-

mon ELISAs, assay for monitoring enzyme

activities and protein and DNA quantifica-

tion assays.

The easy-to-use self-explaining one-view

PhotoRead software lets you get started

with the measurements within seconds.

Up to 6 filters can be used with the instru-

ment enabling the performance of all im-

portant applications as there are

▪ DNA quantification

▪ Protein quantification

▪ Enzyme activities

▪ β-Galactosidase (reporter gene)

▪ Alkaline Phosphatase (ELISA)

▪ Horseradish Peroxidase (ELISA)

Instrument Centro Centro XS3 CentroPRO CentroLIApc CentroLIAPlate formats 96 and 384 wells 96 and 384 wells 96 wells 96 wells 96 wellsSensitivity 5 amol ATP / well

2 zmol fireflyluciferase / well

1.5 amol ATP / well1.5 zmol fireflyluciferase / well

20 amol ATP / well7.5 zmol fireflyluciferase / well

0.002 mIU/L TSH 0.002 mIU/L TSH

Injectors up to 31 in reading position

up to 3 external external external

Heating up to 42 °C ambient ambient ambient ambientShaking 3 modes / 3 speeds 3 modes / 3 speeds 1 mode / 3 speeds 1 mode / 3 speeds 3 modes / 3 speedsSoftware & Operation PC based MikroWin ratio

calculation (DLRTM),kinetics, curve fit automation support

PC based ICE Research incl.ratio calculation(Dual-GloTM)

PC based ICE Immunoassay incl.immunoassay datareduction

on-board sofIAimmunoassay datareductionfor LIA & ILMA

Preferred applications • Aequorin-based Calciummonitoring

• Acridiniumester-basedimmunoassay

• cellular luminescence

• reporter gene assay(single and dual)


• CALUX® Assays

• reporter gene assay(Dual-GloTM)


• immunoassays (LIA): HRP/ALP labelswith luminescent substrates

Mithras LB 940 Multimode ReaderMithras, the extremely versatile multimode readeroffers you all comprehensive technologies used intoday’s research.

AbsorbanceLuminescenceFluorescence (top and bottom)Fluorescence Polarisation (FP)FRETBRET/BRET2

AlphaScreen®

Time-resolved Fluorescence HTRF®

The unit combines fluorescence and luminescen-ce modes with the uncompromised performanceof dedicated instruments. The sensitivity of theluminescence function exceeds even that of dedicated plate luminometers. Up to 4 reagent injectors and temperature controlare the key features for the instrument´s leadership in functional receptor assays based onBRET or Calcium flux monitoring. HTRF® certified,the Mithras can be used for the screening and investigation of biological interactions such askinases or second messengers.

TriStar LB 941 Multimode ReaderThe TriStar LB 941 multimode reader provides youwith outstanding performance and dedicated opti-cal path system as it is known from the Mithras. TriStar offers you thefollowing technologies:

AbsorbanceLuminescenceFluorescenceFRETBRET/BRET2

The instrument can be equipped with up to 4 injectors for fast kinetics and a temperature control for all cellular assays.

Centro / Centro XS3 LB 960 LuminometersThe Centro and Centro XS3 meet all currentlyknown requirements forversatile, robust and sensi-tive microplate luminome-ters. The selected detectorand the proprietary designof the optical system gua-rantee highest efficiency,lowest background andnegligible crosstalk. Centro XS3 has an optimizedoptics for even higher demands on sensitivity.Fulfilling and even exceeding the requirements toqualify as DLReadyTM, the Centro XS3 is the idealinstrument for luciferase reporter gene assays.Three independently controlled injectors withvariable volume give entire freedom in the selec-tion of assay type and assay sequence.

CentroPRO LB 962LuminometerThe CentroPRO microplateluminometer is an attractiveentry-level instrument in research for all glow-type bio-luminescent and chemiluminescent applications. The instrument is controlled via the easy-to-useICE software which includes ratio calculation forreporter gene applications.

detect and identify

Twinkle LB 970FluorometerThe Twinkle LB 970 is based on a dedicated opti-cal system for fluorescence readings from aboveand below a microplate.Various formats from Petridishes to 384 well platesare supported. The Twinkle can beequipped with tempera-ture control to establishideal conditions for all cell based applications.The high quality design is well suited for sensitiveFRET assays.

Artemis K-101HTRF® ReaderArtemis is a dedicated microplate reader specificallydesigned and approved for HTRF® applications. The instrument supports 96 and 384 well microplateformats.Equipped with single photoncounting detection system, ahighly stable Xenon flashlamp, internal reflection optical unit and well optimized filters the Artemis shows enhanced sensitivity. The software is specifically designed forHTRF® measurements.

Stacker LB 931Plate Handling SystemFor medium and higher throughputBerthold Technologies offers theplate handling system Stacker LB 931.A unique feature of the Stacker is the re-stacking operation mode enablinglong-term studies of e.g. reportergene expression.For positive plate identification bar-code readers are available.

Microplate Readers

CentroLIA LB 961 Clinical LuminometerCentroLIA, the stand–alone microplate lumino-meter with integrated soft-ware, is especially designedfor luminescence immuno-assays (LIA). The use ofsoftkeys with an interactivedisplay of their associatedfunction makes the CentroLIAan extremely user-friendly instrument.The Software has been designed with the routineclinical laboratory in mind and offers protocols forall types of immunoassays.

CentroLIApc LB 962Clinical LuminometerThe CentroLIApc is a micro-plate luminometer especiallydedicated to luminescence immunoassays (LIA). The instrument is controlled via the easy-to-usewizard driven ICE software which includes immuno-assay data reduction. The CentroLIApc is an ideal instrument for diagnostic applications and clinical use.


NightOWLTM II LB 983In vivo Imaging SystemGene expression can be monitoredin living organisms with ultra-sens-itive imaging systems. Berthold Technologies contributeda major breakthrough of this tech-nology with its Luminograph LB980 introduced in 1989. Today, thethird generation of low-light ima-gers is available, the NightOWL II.As the NightOWL has been the first imaging instrument, where thecamera is moved inside the cabinet, a lot of special applicati-ons can be done easily like close-up imaging, plant imaging or animal imaging in specific animal beds for multimodal imaging.Orthogonal 3D-imaging for approximate volume calculation is available. Additionally NightOWL II offers quantitative fluores-cence as the unique optical system from the Mithrasmultimode reader has been integrated.

FlowStar LB 513 Radioflow DetectorFlow monitoring of radiolabelled compounds separated by chromatographic techniques has been established as one of the most powerful tools for identification of chemical and biochemicalmetabolites.The LB 513 radiochromatography detection systemcontains componentsfor all alpha, beta,gamma and PET isotopes in drugmetabolism, phar-macology, environ-mental and clinicalapplications. A user-friendly touch screen, the automatic measu-ring cell recognition and the two integrated bipolaranalogue inputs are only some of the features of theFlowStar. Berthold Technologies provides a complete system forradio HPLC applications, including cells, splitters,mixers, pumps and software. A new generation of measuring cells has been designedsuitable for technologies such as UPLC®.

HERM LB 500 High Energy Radio MonitorThe HERM is an additional product in the detectorrange of Berthold Technologiesfor flow-through measurements. The highly sensitive Sodium Iodidecrystal, in conjunction with a leadshielding and Twin-Cell-Changer,enable the detection of variousgamma emitting isotopes.

Multi Crystal LB 2111Gamma CounterThe gamma counterfor clinical diagnosticsand research with 12or 24 detectors meetsthe demands of bigsample loads typicalin gamma countinglaboratories.

Junior LB 9509Portable Tube LuminometerThe Junior is a lightweight portable luminometer specificallydesigned for all common glowtype bioluminescent and chemi-luminescent applications.Rechargeable batteries, as wellas a mains supply and sturdytransportation case, make the Junior an ideal solution for laboratory and outdoor use in research, diagnostics, food and environmental monitoring.

Lumat3 LB 9508Single Tube LuminometerThe Lumat3 tube luminometerprovides the sensitivity andversatility you expect from ageneral purpose luminometer.Advanced digital photoncounting with selected photo-multipliers ensure superiorsensitivity and best match ofspectral demands. Equipped with up to 2 reagentinjectors the Lumat3 is ideally suited for reportergene assays as well as other luminescence applica-tions in research and clinical diagnostics.

AutoLumat Plus LB 953Multi-Tube LuminometerThe universal tube luminometer will be adding aplus to your laboratory. Up to 180 samples placedin a chain can be measured once or sequentiallymany times over a long period.Besides reporter gene assays,ATP monitoring and immuno-assays the AutoLumat is theinstrument of choice for allcellular luminescence applica-tions as the measuring chambercan be temperature controlled.Up to 3 reagent injectors emphasise its versatilityand power.

detect and identify

NightSHADE LB 985 Plant Imaging SystemThe NightSHADE is the first imagingsystem that is fully dedicated tobe used in plant research. Evenlong-term monitoring of lucifera-se or GFP expression is possible.Housed in an absolutely light tight darkroom theNightSHADE’s heart is a cooledCCD camera. It can be moun-ted on top or at the side of thedarkroom to facilitate imaging from above or laterally.A turn table can be applied to take images of a plantfrom different angles with the side-view camera. LED panels in the darkroom can be programmed fordaylight simulation. The darkroom itself may be tem-perature or humidity controlled. Fluoresc ent excitation and emission optics enable themeasurement of multiple fluorescent reporter genesand dyes.

Imaging InstrumentsTube LuminometersRadio HPLC MonitorsGamma Counters


White White clearbottom

Black Black clear bottom

Luminescence x x (x) -Fluorescence (x) - x xAbsorbance - x - xFRET - - x xAlphaScreen® x - - -FluorescencePolarisation

- - x -

Time-ResolvedFluorescence

(x) - x -

HTRF® x - x -

MikroWin 2000The sophisticated software for microplate readersoffers extensive possibilities for instrument operation and data evaluation (kinetics, curve fit,ratio etc.).The wells for measurementand injection can be selectedindependently.

Various operations can be picked and placed in anyorder to accommodate for all different require-ments of the assays to be performed.Kinetic data reduction and graphical display of therespective curves help the user to judge the results.User access and audit trail systems in the Advancedversions are the basis for MikroWin 2000´s 21 CFRpart 11 compliancy.

WinTermWinTerm is a computer–based communication software for a variety of Berthold Technologiesinstruments enabling data download and conversioninto spreadsheet formats.

TubemasterFor the AutoLumat Plus LB953 Tubemaster is a sophisti-cated though easy-to-usesoftware for instrument ope-ration and data evaluationwith GLP documentationfunctions.

LBISBerthold Technologies ́ proven immunoassay dataevaluation software can be used with the Multi CrystalGamma Counter LB2111 and theAutoLumat Plus LB953 including thecontrol of respectiveinstruments.

IndiGOTM

This highly integrated image analysis softwareoffers full functionality for instrument control,image capture and subsequent image analysis forall NightSHADE and NightOWL II systems.In the comfortable andtime-saving analysis partcontrast, transparency andsignal threshold can be easyset with sliders. Afterwardsall settings from one imagecan be applied to all imagesof a whole measurement series. Maintaining originalimages the software can be used in GLP regulatedenvironments.

RadioStarThe dedicated radio chro-matography software meetsall needs for flow monitorcontrol and sophisticatedanalysis of chromatogramsincluding full DPM calculationand contains extensivereport generation functions.

ICE SoftwareInstrument Control and EvaluationThe extremely intuitive software for use with theCentroLIApc, CentroPRO andLumat3 has been designedwith the demands of today’sresearchers as well as theroutine clinical laboratory inmind. Three software versions areavaible:

ICE Research supports ratio calculation for dual reporter gene and ADP/ATP assays as well as kinetics for enzyme activities

ICE Immunoassay includes multiple curve fitting algorithms (spline, linear regression, point-to-point) and options (master and reference curves)

ICE Advanced combines all functions of ICE Researchand ICE Immunoassay versions.

The ease of use during protocol creation, measure-ment and data export has been achieved with awizard-driven and clearly structured software.

detect and identify

Consumables AccessoriesSoftware

MicroplatesVarious plate formats(24, 96 and 384 wells)Black or whiteBlack plates with white wells Clear bottom for cell culture applicationsSterile, cell culture treated (individually packed)

Choosing the right microplate will help you toachieve the best measurement results:

TubesLumi vials 5 mL, 12x75 mm, 3,000 piecesLumi vials 3 mL, 12x47 mm, irradiated, 1,000 piecesLumi vials 3.5 mL, 12x55 mm, 2,000 pieces

Injector cleaning solutionsFor proper maintenance of the injectionsystem, the Cleanit solution is recom-mended for regular cleaning. Daily cleaning ensures that accuracyand precision as well as a long life timeof the injectors will be maintained.

FiltersOnly filters with high trans-mission characteristicsensure best sensitivity.BRET and other colourluminescens applicationsare only possible withextremely efficient opticalsystems.For the best measurement results we offer highquality optical filters and evaluated best filter com-binations.

Excitation and emission filters for fluorescenceapplicationsFilters for absorbance applicationsFilters for luminescence applications Optimized filter sets for BRET, FRET, AlphaScreen®,Fluorescence Polarisation (FP), HTRF®,Time-resolved Fluorescence and Chroma-GloTM

QC testplatesWith the testplates for lumines-cence and absorbance you caneasily check the performance ofyour microplate reader or your imaging instrument

Luminescence testplate for quality controlAbsorbance testplate for quality control

QC Luminescence test kitWith the QC luminescence test kit (label andreagents) the performance ofyour luminometer can bechecked. This is the ideal QC(quality control) method as both,injection and detection system,can be monitored.

Luminescent labelLuminescent reagentsFlash-type reaction


Detect and Identify

Microplate Readersin vivo Imaging InstrumentsTube LuminometersRadio HPLC MonitorsGamma Counters

Bio

anal

ytic

al I

nst

rum

ents

· 0

7-20

10 ·

500

0 ·

Id.N

r. 3

8052

PR2

Rev

. 07

BERTHOLD TECHNOLOGIES GmbH & Co. KG

P.O. Box 100 16375312 Bad WildbadGermany

Phone: +49 7081 177-0Fax: +49 7081 177-100E-mail: [email protected]: www.Berthold.com/Bio

Company profile

Berthold Technologies is considered a worldwideleader in luminescence measuring technology andhas provided thousands of luminometers for rese-arch and diagnostics. The Centro XS3 luminometer for academic andpharmaceutical research has been developed withan optimized optics for even higher demands onsensitivity. The CentroLIApc is an ideal instrumentfor diagnostic applications and clinical use, whilethe new CentroPRO is an attractive alternative forresearch.

In recent years we have expanded the develop-ment of microplate instruments which support newmodern measuring methods. Our multimode readerMithras demonstrates exceptional performance inBRET technology, which is mainly used in researchof G-protein coupled receptors.HTRF® certified, the Mithras can be used for thescreening and investigation of biological interac-tions such as kinases or second messengers.

In gene expression studies, reporter genes havebecome an invaluable tool. Using our extremelysensitive imaging instruments the NightOWL, andthe new NightSHADE, the gene expression can beexamined in living organisms.

NightOWLTM and indiGOTM are trademarks of BERTHOLD TECHNOLOGIES. DLR®, DLReady logo, Dual-GloTM and Chroma-GloTM are registered trademarks ofPromega. HTRF® is a registered trademark of Cisbio. UPLC® is a registered trademarkof Waters. AlphaScreen® is a registered trademark of PerkinElmer. Windows® is a registered trademark of Microsoft. CALUX® is a registered trademark of BioDetection Systems. To run certain applications on these productsmay require licenses from others. Some instruments are not available in all countries! BERTHOLD TECHNOLOGIES reserves the right to implement technical improvements and/or design changes without prior notice.

Some techniques for generating and/or detecting light in biological subjects arepatented and may require licences from third parties. Users are advised to independently determine for themselves whether their activities infringe any valid patent.

detect and identifydetect and identify


Bio

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ytic

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Detect and Identify...Detect and Identify Microplate Readers in vivo Imaging Instruments Tube...

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