Description of Supplementary Files  File Name: Supplementary Information Description: Supplementary Figures and Supplementary Table  File Name: Supplementary Data 1 Description: List of genes differently expressed among the classes passing the cut‐off value in T cells. The genes are tabulated along with parametric p‐value, FDR, the geometric mean of intensities, fold change and links to major annotation sources.  File Name: Supplementary Data 2 Description: List of genes differently expressed among the classes passing the cut‐off value in Monocytes. The genes are Pag. 5 di 5 tabulated along with parametric p‐value, FDR, the geometric mean of intensities, fold change and links to major annotation sources.  File Name: Supplementary Data 3 Description: List of the different gene sets identified in T cell, performing the gene set comparison tool in BRB Array‐Tools as a scoring test to assign the functional category definitions according to the Gene Ontology Database.  File Name: Supplementary Data 4 Description: List of the different gene sets identified in Monocytes, performing the gene set comparison tool in BRB ArrayTools as a scoring test to assign the functional category definitions according to the Gene Ontology Database.  File Name: Peer Review File  

Supplementusing A) AFdimension di

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Supplementary Figure 2. Summary of SPADE analysis. SPADE tree plots of untreated samples (CTRL) for significantly expressed cytokines (IL6, TNFα and MIP1β).

Supplementary Figure 3. Single-cell characterization on gated T lymphocyte subpopulations in GO treated samples (viSNE analysis). Plots show the use of viSNE to obtain a comprehensive single cell view and to distinguish the T cell subpopulations in the GO treated cells. Plots show expression of 9 proteins including cytokines.

cells

Monocytes

mDCs

pDCs

CD16+ NK Cells

CD16- NK Cells

Activated T HelperNaive T Helper

Memory T Helper

Naive T Killer

Memory T killer

Effector T Killer

Activated T killer

mory B cells

Plasma B Cells

Naive B cells

Helper

0

3.24

Monocytes

mDCs

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CD16+ NK Cells

CD16- NK Cells

Activated T HelperNaive T Helper

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Naive T Killer

Memory T killer

Effector T Killer

Activated T killer

Memory B cells

Plasma B Cells

Naive B cells

Effector T Helper

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Monocytes

mDCs

pDCs

CD16+ NK Cells

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Activated T HelperNaive T Helper

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Memory T killer

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Memory B cells

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1 cell9,398.08 cells0

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Effector T Helper

Activated T Helper

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Naïve T Cytotoxic

Effector T Cytotoxic

Activated T Cytotoxic

Memory T Cytotoxic

Plasma B cells

Naïve B cells

mDCs

Memory B cells

NaïveT Helper

pDCs

Monocytes

EffectorT Helper

Activated T Helper

Memory T Helper

Naïve T Cytotoxic

Effector T Cytotoxic

Activated T Cytotoxic

Memory T Cytotoxic

Plasma B cells

Naïve B cells

mDCs

Memory B cells

NaïveT Helper

pDCs

Monocytes

EffectorT Helper

Activated T Helper

Memory T Helper

Naïve T Cytotoxic

Effector T Cytotoxic

Memory T Cytotoxic

Plasma B cells

Naïve B cells

mDCs

Memory B cells

Activated T Cytotoxic

3.15

Lymphocytes T Helper

Lymphocytes T Cytotoxic

Activated

Memory

Effector

Naive

CD27 CD45RA CD38 HLADR

IFNg IL2 IL4 IL5 TNFa

CD27 CD45RA CD38 HLADR

Activated

Memory Effector

Naive

IFNg IL2 IL4 IL5 TNFa

Supplementary Figure 4. Single-cell characterization of gated T lymphocyte subpopulations in GONH

2 treated

samples (viSNE analysis). Plots show the use of viSNE to obtain a comprehensive single cell view and to distinguish the T cell subpopulations in the GONH

2 treated cells. Plots show expression of 9 proteins including cytokines.

Lymphocytes T Helper

Lymphocytes T Cytotoxic

CD27 CD45RA CD38 HLADR

Activated

Memory

Effector

Naive

IFNg IL2 IL4 IL5 TNFa

CD27 CD45RA CD38 HLADR

Activated

Memory Effector

Naive

IFNg IL2 IL4 IL5 TNFa

Supplementary Figure 5. Single-cell characterization of GO and GONH

2 on gated B cells (viSNE analysis). Plots

show the use of viSNE to obtain a comprehensive single-cell view and to distinguish the B cell subpopulations in the GO and GONH

2 treated cells. Plots show expression of the 7 proteins used to distinguish the single population

including cytokines.

B Cells

GONH2

GO

HLADR CD27 CD38

Memory

Plasma Naive

IL2 IL4 IL5 TNFa

CD38

Memory

Plasma Naive

HLADR CD27

IL2 IL4 IL5 TNFa

Supplementary Figure 6. Single-cell characterization of gated NK cells and monocytes in GO and GONH

2

treated samples (viSNE analysis). Plots show the use of viSNE to obtain a comprehensive single-cell view and to distinguish the NK cells and monocytes in GO and GONH

2 treated cells. Plots show expression of 9 proteins including

cytokines for NK cells and 6 for monocytes.

NK Cells

Monocytes

CD16 HLADR CD27 CD38

CD14 CD16 HLADR CD38 TNFa IL6

IL4 IL5 TNFa

GO

NK Cells

Monocytes

GONH2

CD14 CD16 HLADR CD38 TNFa IL6

CD16 HLADR CD27 CD38

IL4 IL5 TNFa

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