Derrick R. J. Kolling March 16, 2010 The Chemistry of Photosynthetic Water Oxidation: From...
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Transcript of Derrick R. J. Kolling March 16, 2010 The Chemistry of Photosynthetic Water Oxidation: From...
Derrick R. J. Kolling March 16, 2010
The Chemistry of Photosynthetic Water Oxidation: From Fundamentals to Biofuels
Kolling LaboratoryCurrent Members
Yasmine Zeid
Ben Blodgett
Cameron Buchman
Courtney Nichols
James Board
Rebecca Mead
Shaheed Elhamdani
Evan Riley
Matthew Thompson
Former Members
Chase Turner
The lab has three main projects:
1. Assembly of the oxygen-evolving complex
2. [Mn2+] in compartments of phototrophs
3. Metabolomic studies of Chlorella vulgaris
We are looking for students who:
1. Would like to work >3 semesters
2. Are interested in writing grants
3. Enjoy challenging projects
• Psychrophile, 12°C• Antarctic benthic mat
Oscillatoria sp.
• Thermophile, 55°C• PSII crystal structure• Genome sequenced
Thermosynechococcus elongatus
www-dsv.cea.fr/var
www-biol.paisley.ac.uk
cc
cc
Project 1: OEC Photoassembly
We are studying photoassembly in photosynthetic extremophiles that grow at very different temperatures.
Project 2: Compartmental [Mn2+]
We are working to determine the concentration of Mn2+ in the different compartments of phototrophic prokaryotes and eukaryotes. This will help us to determine the mechanism of photoassembly.
Chlamydomonas reinhardtii
CytoplasmStromaLumen
Chloroplast
Cytoplasm
Lumen
[Mn2+]?
Synechocystis sp. PCC 6803
Project 3: Metabolomics
We are developing a strategy to determine the partial metabolome of the lipid generation pathways in Chlorella vulgaris. This organism and its metabolism are of interest, because its lipids can be used to make biodiesel.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Barber & Iwata (2004) Science
A trip from a leaf to the oxygen-evolving complex.
Ferreira et al. (2004) Science
The oxygen-evolving complex!
How do we probe photosynthesis?
• O2 production
• Fluorescence
• S-State Trapping• EPR Spectroscopy