Department of Nutritional Sciences University of Toronto
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Ahmed El-Sohemy Department of Nutritional Sciences University of Toronto Gene Polymorphisms and dietary preferences
Transcript of Department of Nutritional Sciences University of Toronto
Ahmed El-Sohemy
Department of Nutritional Sciences University of Toronto
Gene Polymorphisms and dietary preferences
Eny and El-Sohemy, 2010
Eny and El-Sohemy, 2010
GLUT2
Glucose Transporter Type-2 (GLUT2)
Kidney
Brain
Glucose Homeostasis
Liver
Pancreas
Small Intestine
110 Thr Ile GLUT2 Protein
– 109 men and women (36-75 years) – Overweight/obese with mild type 2
diabetes – Diet: 3-day food records (repeated 2
weeks later)
Study Population
Protein
Visit 1
Thr/Thr Thr/Ile + Ile/Ile0
25
50
75
100 91 g 95 g
Genotype
Prot
ein
(g/d
)
Visit 2
Thr/Thr Thr/Ile + Ile/Ile0
25
50
75
100 92 g 93 g
GenotypePr
otei
n (g
/d)
P= 0.46 P= 0.87
Eny et al., Physiol. Genomics, 33:355-60, 2008
Fat
Visit 1
Thr/Thr Thr/Ile + Ile/Ile0
25
50
75
100
70 g76 g
Genotype
Fat (
g/d)
Visit 2
Thr/Thr Thr/Ile + Ile/Ile0
25
50
75
100
69 g 68 g
GenotypeFa
t (g/
d)
P= 0.37 P= 0.95
Eny et al., Physiol. Genomics, 33:355-60, 2008
Replication Study
• Population 1 – 109 men and women (36-75 years) – Overweight/obese with mild type 2 diabetes – Diet: 3-day food records (repeated 2 weeks later)
• Population 2 – 954 young men and women (20-29 years) (Toronto Nutrigenomics and Health Study) – Mostly lean and healthy – Diet: Food Frequency Questionnaire
Sugar
Thr/Thr Thr/Ile + Ile/Ile
100
125
150
115 g
131 g
Genotype
Suga
rs (g
/d)
P= 0.007
Eny et al., Physiol. Genomics, 33:355-60, 2008
Protein and Fat
Thr/Thr Thr/Ile + Ile/Ile0
25
50
75
10086 g 85 g
Protein
Genotype
Pro
tein
(g/
d)
P= 0.92
Thr/Thr Thr/Ile + Ile/Ile0
25
50
75 66 g70 g
Fat
GenotypeFa
t (g/
d)
P= 0.15
Eny et al., Physiol. Genomics, 33:355-60, 2008
Eny et al., Physiol. Genomics, 33:355-60, 2008
Eny and El-Sohemy, 2010
GLUT2
Eny and El-Sohemy, 2010
GLUT2 DRD2
Carriers of the Val allele consume less sugar.
Eny and El-Sohemy, 2010
GLUT2 DRD2 TAS1R2
Sensory Evaluation Subjects
• N =118 • Caucasian, Age 20-33, Non Smokers
Solutions
• NaCl, Sucrose, Citric Acid, PROP, FAs
Visit 1- Threshold Testing Method: 3 Alternative Forced Choice Staircase
Visit 2-Supra-threshold Testing
Method : Scaling with the general Limited Magnitude Scale
Genome Wide Association Scans
Affymetrix 6.0 chip • 906,000 SNPs • 946,000 Copy Number
Variation probes Subjects • n=550 • Caucasian
Garcia-Bailo et al., OMICS, 13:69-80, 2009
Implications
Health Outcome (+/-)
Diet
Genotype
ACKNOWLEDGMENTS
Karen Eny Marilyn Cornelis Bénédicte Fontaine-Bisson Ilana Platt Stephen Ozsungur Leah Cahill Clare Toguri
Canada Research Chairs
Canadian Institutes of Health Research
Natural Sciences and Engineering Research Council
Advanced Foods and Materials Network Centres of Excellence
Hyeon-Joo Lee Lindsay Stewart Daiva Nielsen Alejandra Navarro-Allende Susana Huang Sarah Herd Darren Brenner Bibiana Garcia-Bailo
Tom Wolever Lisa Duizer Paul Corey Hannia Campos David Jenkins
Canadian Diabetes Association
