Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites...

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Ministry of Higher Education and Scientific research Directorate of Quality Assurance and Accreditation ب ڕێوي ب رایتماو جۆری و میای دڵىی تی ب خشیهDepartment of Medical Laboratory Technology College of Erbil Health Technical College University of Erbil Polytechnic University Subject: Medical Parasitology Course Book: second stage (year 2) Lecturer's name: Dr. Hemdad Hawez Mawlood (PhD in Medical and Molecular parasitology) Academic Year: 2019/2020

Transcript of Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites...

Page 1: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

Department of Medical Laboratory Technology

College of Erbil Health Technical College

University of Erbil Polytechnic University

Subject: Medical Parasitology

Course Book: second stage (year 2)

Lecturer's name: Dr. Hemdad Hawez Mawlood (PhD in Medical and

Molecular parasitology)

Academic Year: 2019/2020

Page 2: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

Course Book

1. Course name Medical Parasitology

2. Lecturer in charge Dr.Hemdad Hawez Mawlood

3. Department/ College MLT/Erbil Health Technical College

4. Contact E-mail: [email protected]

Tel: 009647504489480

5. Time (in hours) per week Theory: 2

Practical: 3

6. Office hours 11 hours

7. Course code

8. Teacher's academic

profile

Currently Ph. D in Molecular Parasitology between University

of zakho (Kurdistan)Tennessee State University(USA) and

University of Tennessee at Knoxville(USA).

From 10/2004 to 2/2007 Master in Medical Parasitology

University of Alqadisiya (South of Iraq).

From 10/1994 to 7/1998 Bachelor of Science in Biology from

University of Salahaddin.

From 2017 –till now lecturing Clinical Parasitology for second

grade in MLT Dep/Erbil Health Technical college.

From 10/2012 to 07/2014 lecturing Medical parasitology for

second grade in Erbil Medical Technology Institute

Erbil, Kurdistan and Researching for PhD at University of

Zakho.

From 11/2014 to 5/2015 researching and study in Tennessee

State University and Southern Hills Medical Centres in United

states of America.

From 3/2016 to 6/2016 researching and training PCR-RFLP

Technique for genotyping; Mice injection and cell culture for

T. gondii at University of Tennessee at Knoxville/USA

From 10/ 2010 to 10/2012 researching and working as

MICROBIOLOGIST 2 Certified at Tennessee Health

Department Laboratory Service/USA for following issues

1. Surveillance for the confirmation of: Streptococcus

pnumoniae, Haemophilus influenza and typing, Neisseria

meningitides and grouping, Streptococcus pyogenes (Group A

Page 3: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

Streptococci) and Staphylococcus aureus.

2.Screened for sexually transmitted diseases by Nucleic Acid

Amplification Techniques by Genprobe; specifically, Chlamydia

trachomatis and Neisseria gonorrhoeae.

3.detected microorganisms in water samples and wastewater, the

methods were performed by Environmental Protection Agency

(EPA). the following organism were detected: Escherichia coli,

Fecal coliform, Enterococcus species, Heterotrophic bacteria,

Cryptosporidium and Giardia lumblia

9. Keywords Common parasites in Kurdistan , Protozoa parasites,

worms,Ectoparasite

10. Course overview:

I Explain the view of course by following points:

1.parasitology classes it is important for each student of our department because in future

when the student graduated in college and get Bachelor’s degree in Medical Laboratory

Technology he or she doesn’t have chance to find out the job without parasitic diagnosis

skills.

2.The principle of relation between parasite and hosts it is a major part of this classes

especially human beings because human it has considered an intermediate host of many

parasites.

3. during the course student should be understood the major type of protozoa parasites and

worms especially all parasites which are available in Kurdistan, also should be know the type

of hosts and mechanism of pathogenicity of each parasite.

4.Diagnostic of parasites it is topic of each subjects and follow up new techniques for

diagnostic of parasites especially molecular technique and genotyping of parasites.

5.After graduation all students just need it minor review of techniques in general hospitals

and private labs. 11. Course objective:

During Medical parasitology classes student, they learnt and earned knowledge of:

1.the life cycle, lab diagnostic, pathogenicity, treatment infective stage, intermediate and

final host of most protozoa parasites.

2. life cycle, lab diagnostic, number of intermediate hosts, final host, and treatment of each

cestoda, Nematode and other type of parasite worms

3.diffrent between Ectoparasits and endoparasites and pathogenicity of Ectoparasites with the

life cycle and diagnostic of Ectoparasites in Kurdistan region/Iraq

12. Student's obligation For each student is obligated to know:

1.draw and label all stages of parasites in lab.

2.bring fresh samples of blood, urine, sputum and stool to lab. for diagnostic common

parasites in Erbil such as Giardia lumblia and Entamoeba histolytica in stool, Plasmodium sp

in blood, Trichomonas vaginalis in urine.

3.Atending all students in theory and practical classes are obligated

Page 4: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

13. Forms of teaching Updating lectures by Power points, using white board during explanation like life cycle and

scientific name of parasites also practical parasite book it be available in lab to see image of

each parasites and compare with the parasites in microscope

14. Assessment scheme

Each lecture especially practical lecture the quiz is requirement in next week and monthly

seminar is requirement with the scores. The seminar is run by two or three students. Also,

monthly and final semester exams are required.

15. Student learning outcome: The outcome of this learning are:

1.students know how diagnosis most and common parasites by different techniques

especially immunology and molecular techniques both of techniques available in private

labs.

2.Students know what type of samples used for detecting parasites and using concentration

method to detection ova of worms from stool.

3.during detecting parasites in fresh samples. preparation and fixed those parasites in slide by

Canada balsam for keeping those slide in parasitology lab for next stage students.

4.using Realtime –PCR for detecting common parasites in clinical lab like T. gondii, E.

histolytica, Giardia lamblia…etc.

16. Course Reading List and References‌:

▪ Using diagram of life cycles from CDC (centre of control disease and control) website and

using those books below:

1.ELlizabeth A. Zeibig (2013.) Clinical Parasitology a Practical Approach 2nd

edition.

Elsevier St. Louis, Missouri, USA.

2. John W. Ridley. (2012). Parasitology for Medical and Clinical Laboratory Professionals.

Delmar, Cengage Learning, USA.

3. Larry, R.; John, J and Steve, N (2013). Foundations of Parasitology .9 edition

McGraw Hill.

Florida USA.

4.Using different Journals about medical parasitology such as: Journal of Bacteriology &

Parasitology, Applied parasitology, Experimental parasitology, Parasitology international,

Korean Journal of parasitology and European Journal of Parasitology with other international

Journals.

.

Page 5: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

17. The Topics and No. of Lectures Lecturer's name

(2hrs.) 1.Parasitic diseases continue to be a major public health

problem all over the world with associated high degree of

mortality and morbidity

2.Host and type of parasite and type of relation between two

organism and source of parasite infection,

3.explian portal to entrance the parasites to human bodies.

4. A parasite may live in or on the tissues of its host without

causing evident harm. However, in majority of cases the parasite

has the capacity to produce damage.

5. Immunological protection against parasitic infections is much

less efficient than it is against bacterial and viral infections.

6.Explian type of samples and lab techniques for detecting stage

of parasites such as ova of worms, Trophozoite and cyst of

protozoa parasites.

7.Explian method of prevention and medicines against parasite

8.Explian all phylum of protozoa parasites such as:

Sarchomastigophora, Apicomplexia and ciliophoran.

9.Explian all Amoeba of intestine and different between them

10.explian common flagellate’s parasites like Giardia lumblia,

Trichomonas vaginalis, Chilomastix mesinlii

11.explian Balantidum coli only parasite related to ciliophora

Introduction of

parasitology 8/9/2019

Define of parasitology

and type parasite and

hosts

15/9/2019 How parasite enter the

body?

22/9/2019

Pathogenicity of

parasites

29/9/2019

Immunity of parasite

6/10/2019

Laboratory diagnosis of

parasites

13/10/2019

Treatment and

prevention of parasite

infection

19/10/2019

Taxonomy of parasites

26/10/2019

Intestinal protozoa

3/11/2019

Mastigophora (Flagellata

10/11/2019)

Ciliophora

17/11/2019

Haemoflagellates

Page 6: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

12.explian all type of Trypanosoma and Leishmania

13.Explian all type of plasmodium sp

14.Explian T. gondii and detection of toxoplasmosis

15. Explain general characteristics of Nemathelminthes, Platyhelminthes and Trematodes

16.Explian life cycle, morphology, pathogenicity and method of

transmission for Pin worm.

17.Explain life cycle, pathogenicity, morphology, method of

transmission and diagnostic of Ascaris Lumbricoides

18. Explain life cycle, pathogenicity, morphology and method of

transmission of Trichuris trichiura.

19. Explain life cycle, pathogenicity, morphology and method of

transmission for both. Ancylostoma duodenale

and Necator Americans and different between them.

20. Explain life cycle, pathogenicity, morphology and method of

transmission of Strongyloides stercoralis

21. Explain life cycle, pathogenicity, morphology and method of

transmission of Trichinella spiralis

22. Explain life cycle, pathogenicity, morphology and method of

transmission of Wuchereria bancrofti.

23. Explain life cycle, pathogenicity, morphology and method of

transmission of Echinococcus granulosus

25/11/2019

Apicomplexa

14/12/2019

Toxoplasmosis

21/12/2019

Helminths (worms)

28/12/2019

Pin worm (Enterobius

vermicularis)

7/1/2020

Ascaris Lumbricoides

14/1/2020

Trichuris trichiura

(Whipworm)

21/1/2020

Ancylostoma duodenale

and Necator Americans

28/1/2020

Strongyloides stercoralis

5/2/2020

Trichinella spiralis

12/2/2020

Filariasis ex. Wuchereria bancrofti

19/2/2020

Morphology and

classification of

Tapeworm and Hydatid

cyst

Page 7: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

24. Explain life cycle, pathogenicity, morphology and method of

transmission of Taenia saginata

25.Explain life cycle, pathogenicity, morphology and method of

transmission of Teania solium and differentiation with theTeania

saginata

26.Explain life cycle, pathogenicity, morphology and method of

transmission of Diphyllobotrium latum

27.Explain life cycle, pathogenicity, morphology and method of

transmission of mite

28. Explain life cycle, pathogenicity, morphology and method of

transmission of tick

26/2/2020

Beef Tape worm

3/3/20

Pork tape worm

10/3/2020

Fish tape worm

3/4/2020

Ectoparasites

10/4/2020

Ectoparasites

17/4/2019

18. Practical Topics Lecturer's name

3 hrs. 1.Lab requirements and define some terms related with

parasitology lab and type of samples for detecting parasites.

2.Describe morphology of cyst and trophozoite, infective stage

and lab diagnosis and show slide to student of both stage

3.Describe morphology of cyst and trophozoite, infective stage

and lab diagnosis and illustrated both stages by slide then

differentiation with E. histolytica.

4.Explian other amoebas with the stages and illustrated each one

by slide.

5. Describe morphology of cyst and trophozoite, infective stage

and lab diagnosis and show slide to student of both stage.

6.Explian Direct method (General Stool Examination)

How fill out the form of GSE in hospitals and private labs.

7.Flotation method with the procedure

Introduction of

parasitology lab

14/9/2019

Entamoeba histolytica

21/9/2019

Entamoeba coli (non-

pathogenic)

28/9/2019 Other Amoebas 5/10/2019 Giardia lamblia

12/10/2018 19/10/2019 Fresh stool sample

by PVA

Concentration method

Page 8: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

8.Sedimentation method

9.Trpanosoma species, morphology of each genus then illustrated

by slides.

10.Lieshmania spices, morphology of each genus then illustrated

by slides.

11.Describe morphology of cyst and trophozoite, infective stage,

lab diagnosis and show slide to student of both stage

12.Explian GUE and vaginal swab for detecting Trichomonas

vaginalis.

13. One step for TORCH

14.Immunochromatography (rapid test) for T. gondii IgG and

IgM.

15.ELISA and ELIFA for detecting T. gondii in Kurdistan.

16. Describe morphology of stages, lab diagnosis and illustrated

by slides.

17. Describe morphology of Enterobius vermicularis and lab

diagnosis with the illustrated by slides.

18. Describe morphology of Ascaris lumbricodes and lab

diagnosis with the illustrated by slides.

19. Describe morphology of Echinococcosis granulosis, lab

diagnosis and illustrated all stages by slides. Also determine

infective stage

26/10/2019

Concentration method

3/11/2019

Haemoflagellata

10/11/2019

Haemoflagellata

17/11/2019

Balantidium coli

24/11/2019

GUE and vaginal swab

1//12/2019

Immunological

technique for detecting

parasites

8/12/2019

Immunological

technique for detecting

parasites

15/12/2019

Immunological

technique for detecting

parasites.

22/12/2019

Malaria

29/12/2019

Helminths (Pinworm)

10/1/2020

Giant roundworm 17/1/2020

Tapeworm

25/1/2020

Page 9: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

20.see hydatid cyst in slaughters among different intermediate

host

21. Describe morphology of Teania saginata, lab diagnosis and

determine infective stage with illustration of all stages by slide.

22. Describe morphology of Teania solium, lab diagnosis and

determine infective stage with illustration of all stages by slide

then differentiation with Teania saginata.

23.Diffrent egg of worms with psedoparasites

24. Describe morphology of Wuchereria bancrofti, lab diagnosis

with illustration by slide.

25.Describe morphology of Ancylostoma duodenale and lab

diagnosis with illustration by slide.

26. Describe morphology of Necator Americans and lab diagnosis

with illustration by slide,

27. Describe morphology Strongyloides stercoralis

of and lab diagnosis with illustration by slide,

28. Describe morphology mite

of and lab diagnosis with illustration by slide,

29. Describe morphology tick

of and lab diagnosis with illustration by slide,

Visiting slaughter

2/2/2020 Beef tape worm 9/2/2020 Pork tape worm 17/2/2020 24/2/2020 Psedoparasites Filiariasis 2/3/2020

Ascaris lumbricods

Ancylostoma duodenale

9/3/2020

Necator Americans

1/4/2020

Strongyloides stercoralis

8/4/2020 Ectoparasites 15/4/2019 Ectoparasites 22/4/2020

Page 10: Department of Medical Laboratory Technology parasitology sylibus.pdf · diagnostic of parasites especially molecular technique and genotyping of parasites. 5.After graduation all

Ministry of Higher Education and Scientific research

Directorate of Quality Assurance and Accreditation خشیه‌ب‌تی‌دڵىیایی‌جۆری‌و‌متماو‌رای‌ب‌ڕێوي‌ب

19. Examinations: 1. Compositional: In this type of exam the questions usually starts with Explain how, What are the reasons for…?, Why…?, How….? With their typical answers Examples should be provided

2. True or false type of exams:

In this type of exam a short sentence about a specific subject will be provided, and then students will comment on the trueness or falseness of this particular sentence. Examples should be provided

3. Multiple choices: In this type of exam there will be a number of phrases next or below a statement, students will match the correct phrase. Examples should be provided.

20. Extra notes: Here the lecturer shall write any note or comment that is not covered in this template and he/she wishes to enrich the course book with his/her valuable remarks.

21. Peer review ڵ‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌ی‌هاوه‌وه‌پێداچوونه This course book has to be reviewed and signed by a peer. The peer approves the contents of your course book by writing few sentences in this section. (A peer is person who has enough knowledge about the subject you are teaching, he/she has to be a professor, assistant professor, a lecturer or an expert in the field of your subject).

ود‌بکات‌و‌‌س‌پ‌‌ک‌کاوی‌کۆرس‌ت‌ڕۆکی‌باب‌اويیر‌بکرێت‌و‌و‌س‌‌وي‌کادیمی‌ڵێکی‌ئ‌ن‌اوي‌الی‌بێت‌ل‌دي‌‌م‌کۆرسبووک‌ئ

.بکات‌ر‌س‌ل‌و‌واژووی‌ک‌ڕۆکی‌کۆرس‌ر‌شیاوی‌واوي‌س‌ک‌بىووسێت‌ل‌ی‌شود‌وو‌ج .بێت‌متر‌و‌مامۆستا‌ک‌‌ی‌زاوستی‌ل‌بیت‌پل‌و‌دي‌‌ک‌ر‌کۆرس‌س‌بێت‌ل‌زاویاری‌‌‌ک‌‌ی‌س‌و‌ک‌ڵ‌ئ‌اوي

‌Review by:

Assistant proff Karwan salao Najm

MLT DEP