B. K. Birla College (Autonomous), Kalyan

Department of Biotechnology

S.Y.B.Sc.

Syllabus for Autonomy

Year

2018-19

S.Y.B.Sc. SEMESTER III Credits

BUSBT301 Biophysics 02

BUSBT302 Applied chemistry I 02

BUSBT303 Immunology 02

BUSBT304 Cell biology and cytogenetics 02

BUSBT305 Molecular biology 02

BUSBT306 Applied Microbiology 02

BUSBT307 Research methodology 02

Practical

BUSBTP301 Practical based on Paper I and II 02

BUSBTP302 Practical based on Paper III and IV 02

BUSBTP303 Practical based on Paper V and VI 02

SEMESTER IV

BUSBT401 Biochemistry 02

BUSBT402 Applied chemistry II 02

BUSBT403 Immunology 02

BUSBT404 Environmental Biotechnology 02

BUSBT405 Bioprocess technology and Biostatistics 02

BUSBT406 Applied Biotech 02

BUSBT407 Enterpreneurship Development 02

Practical

BUSBTP401 Practical based on Paper I and II 02

BUSBTP402 Practical based on Paper III and IV 02

BUSBTP403 Practical based on Paper V and VI 02

SEMESTER III SEMESTER IV

Biophysics Biochemistry

Unit I Introduction to optics and lasers Carbohydrate Metabolism, ETS and

Energy Rich Compounds

Unit II Heat, sound, magnetism and

fluid dynamics

Amino Acid Metabolism

Unit III Electrophoretic techniques Lipids and Nucleotide metabolism

Applied chemistry I Applied chemistry II

Unit I Organic Chemistry Sampling and separation techniques

Unit II Synthesis of organic compounds Natural product chemistry

Unit III Green Chemistry and synthesis Polymers and nanomaterials

Immunology Immunology

Unit I Effectors of immune response Infectious Disease

Unit II Cell receptors Medical Microbiology- causative

organism

Unit III Immunotechniques Introduction to Molecular Diagnostics :

Cell biology and cytogenetics Environmental Biotechnology

Unit I Cytoskeleton Environmental Pollution

Unit II Cell membrane Global Environmental Problems and

Issues

Unit III Cytogenetics Bioremediation

Molecular biology Bioprocess technology and

Biostatistics

Unit I Gene expression-Transcription Microorganisms in industrial processes

Unit II Gene expression- Translation Fermentation processes and fermentor

Unit III Regulation of Gene expression Biostatistics

Applied Microbiology Applied Biotech

Unit I Air microbiology Plant Tissue Culture

Unit II Potable water and waste water

microbiology

Animal Tissue Culture

Unit III Food microbiology

Scientific writing and Communication

Skills

Research methodology Enterpreneurship Development

Unit I Introduction to research

methology and research problem

Introduction to Entrepreneurship

Development

Unit II Research design and data

collection

Setting-up of an Enterprise and Planning

Unit III Interpretation and report writing Marketing, Sales, Advertising and

International Market research

SEMESTER III Credits

BUSBT301 Biophysics 02

Unit I Introduction to optics and lasers

1.1 Optics :Properties of Light - Reflection,

Refraction, Dispersion, Interference.

1.2 Lasers :Properties of Lasers, Stimulated

Emissions, Laser Action; Applications of

Laser.

1.3 Electromagnetic Radiations:Introduction to

Electromagnetic Radiation.

1.4 Spectroscopy :Types and Properties of

Spectra; Basic Laws of Light Absorption.

Spectrophotometer:-Principle,

Instrumentation and Applications.

15

lectures

Unit II Heat, sound, magnetism and fluid dynamics

2.1 Concept of Temperature; Modes of Heat

Transfer; Measuring Temperature; Platinum

Resistance Thermometer; Thermocouple

and Thermistors.

2.2 Sound:Types of Sound Waves - Audible,

Ultrasonic and Infrasonic Waves; Doppler

Effect;Applications of Ultrasonic Waves.

2.3 Magnetism: Magnetic Field; Magnetism of

Earth; Paramagnetism, Diamagnetism,

Ferromagnetism.

2.4 Nuclear Magnetism and Biomagnetism.

2.5 Fluid Dynamics :Viscosity: Definition Flow

of Liquids through Capillaries; Stokes’

Law; Terminal Velocity. Determination of

‘η’ by Falling Sphere Method; Viscosity

Estimation by Oswald’s Viscometer.

2.6 Surface Tension: Definition - Surface

Tension and Surface Energy; Capillary

Action; Angle of Contact; Wettability;

Temperature Dependence of Surface

Tension. Applications in Biology.

15

lectures

Unit III Electrophoretic techniques

3.1 Migration of Ions in an applied electric

field;Factors affecting Electrophoretic

Mobility; Moving Boundary Electrophoresis;

Principle of Electrophoresis; Supporting

Matrix;

3.2 Paper Electrophoresis; AGE; Native and

SDS PAGE (reducing and non-reducing,

continuous and discontinuous);.

3.3 Staining and Detection Methods; Gel-

Documentation. Applications in Biology.

Ref:

Methods in Molecular Biophysics, Igor N S, N

Zaccai & J Zaccai, (2007) Cambridge

Advanced Methods in Protein Microsequencing,

Witmann

Essential Biophysics, Narayanan, New Age

Publications

Handbook of Molecular Biophysics (Methods &

Application), 2009, HG Bohr, Wiley

Principles & techniques of Biochemistry &

Molecular Biology, Wilson & Walker.

BUSBT302 Applied chemistry I 02

Unit I Organic Chemistry

1.1 Introduction to Types of Organic

Reactions :Addition, Elimination and

Substitution Reactions.

1.2 Essential and Non-essential Elements in

Biological Systems.Role of Metal Ions in

Biological Systems.

1.3 Metal Coordination in Biological Systems

: Enzymes, Apoenzymes and Coenzymes.

1.4 Biological Role of Metalloenzymes wrt

Myoglobins, Haemoglobin. Biological

Role of Carboxypeptidases, Catalases

and Peroxidases.

1.5 Structure and Function: Dioxygen

Binding, Transfer and Utilization; Metal

Complexes in Medicines.

15

lectures

Unit II Synthesis of organic compounds

2.1 Criteria for Ideal Synthesis; Selectivity

and Yield. Linear and Convergent Synthesis

and Multicomponent Reactions.

2.2 Microwave Assisted Organic Synthesis,

Ultrasound in Synthesis and Polymer

supported Synthesis. Retrosynthesis

15

lectures

Unit III Green Chemistry and synthesis

3.1 Introduction to Green Chemistry; Need and

Relevance of Green Chemistry; Principles of

Green Chemistry.

3.2 Green Synthesis in Industry: Green

Materials, Green Reagents, Green Solvents

and Green Catalysts.

15

lectures

Ref:

Organic Chemistry, R.T. Morrison, R.N. Boyd

and S.K. Bhatacharjee, 7th Edition, Pearson

Education (2011).

Organic Chemistry, T.W.G. Solomon and C.B.

Fryhle, 9th Edition, John Wiley & Sons, (2008)

A guide to mechanism in Organic Chemistry, 6th

Edition, Peter Sykes, Pearson

Education

Fundamentals of Organic Chemistry , G. Marc

Loudon, 4th Edition Oxford

Organic Chemistry, L.G. Wade Jr and M.S.

Singh, 6th Edition,2008.

Organic Chemistry, Paula Y. Bruice, Pearson

Education, 2008

Organic Chemistry, J.G. Smith, 2nd Edition

Special Indian Edition, Tata 21 McGraw Hill

Organic Chemistry, S.H. Pine, McGraw Hill

Kogakusha Ltd

BUSBT303 Immunology 02

Unit I 1.1 Cells of immune system –lymphoid cells, NK

cells, mononuclear phagocytes, granulocytic

cells

1.2 Antigens and Antibodies: Properties of

antigen, adjuvant, epitopes.

1.3 Basic structure classes & sub- classes of

antibodies with their biological activity

(tabulated), antigenic determinants on

immunoglobulins.

15

lectures

Unit II Effectors of immune response

2.1 Haematopoiesis; Cells of the Immune

System; Primary and Secondary Lymphoid

Organs.

2.2 Complement System- Classical, Alternate

and Lectin; Regulation and Biological

Effects of Complement System;

Deficiencies of Complement System

15

lectures

Unit III Immunotechniques

3.1 Precipitation Reactions

:Immunoprecipitation,

Immunoelectrophoresis, CIEP, Rocket

Electrophoresis and 2-D

Immunoelectrophoresis.

3.2 Agglutination Reactions :

Passive, Reverse Passive, Agglutination

15

lectures

Inhibition.

Coomb’s Test;

Ref:

Kuby immunology, Judy Owen , Jenni Punt ,

Sharon Stranford., 7th edition (2012), Freeman

and Co., NY

Textbook of basic and clinical immunology, 1st

edition (2013), Sudha Gangal and Shubhangi

Sontakke, University Press, India

Immunology, 7th edition (2006), David Male,

Jonathan Brostoff, David Roth, Ivan Roitt,

Mosby, USA.

Introduction to Immunology- C V Rao- Narosa

Publishing House

BUSBT304 Cell biology and cytogenetics 02

Unit I Cytoskeleton

1.1 Overview of the Major Functions of

Cytoskeleton.Microtubules: Structure and

Composition. MAPs: Functions- Role in

Mitosis, Structural Support and

Cytoskeleton Intracellular Motility.

1.2 Motor Proteins: Kinesins, Dynein; MTOCs.

Dynamic Properties of Microtubules.

1.3 Microtubules in Cilia and Flagella.

1.4 Microfilaments: Structure, Composition,

Assembly and Disassembly.

1.5 Motor Protein: Myosin. Muscle Contractility:

Sliding Filament Model. Actin Binding

Proteins : Examples of NonMuscle

Motility. Intermediate Filaments :Structure

and Composition; Assembly and

Disassembly;Types and Functions.

15

lectures

Unit II Cell membrane

2.1 Uptake of Nutrients by Prokaryotic Cells;

Cell Permeability.Principles of Membrane

Transport. Transporters and Channels; Active

Transport, Passive Transport; Types of

Transporters; Types of ATP Driven Pumps -

Na+ K+ Pump.

2.2 Cell Junctions; Cell Adhesion and

Extracellular Material Microvilli; Tight

Junctions, Gap Junctions; Cell Coat and Cell

Recognition.Cellular Interactions.

15

lectures

Unit III Cytogenetics

3.1 Cytogenetics :Structure of Chromosome -

15

lectures

Heterochromatin, Euchromatin, Polytene

Chromosomes.

3.2 Variation in Chromosomal Structure and

Number : Deletion, Duplication, Inversion,

Translocation, Aneuploidy, Euploidy and

Polyploidy and Syndromes- Klinefelter,

Turner, Cri-du-Chat, Trisomy -21, Trisomy

18 and Trisomy 13.

3.3 Sex Determination and Sex Linkage :

Mechanisms of Sex Determination (XX-

XY, ZZ-ZW, XX-XO)

3.4 Dosage Compensation and Barr Body.

Genetic Linkage, Crossing Over and

Chromosomal Mapping :

3.5 Tetrad Analysis; Two-point Cross; Three

point Cross; Pedigree Analysis.

Ref:

Cell and Molecular Biology- Concepts and

Experiments—Karp – Wiley International.

iGenetics- Peter Russell -Pearson Education

Microbial Genetics- Freifelder –Narosa

Publishing House

Genes XI, 11th edition (2012), Benjamin Lewin,

Publisher - Jones and Barlett Inc.USA

BUSBT305 Molecular biology 03

Unit I Gene expression-Transcription

1.1 Gene Expression- an Overview.

Transcription Process in Prokaryotes :RNA

Synthesis; Promoters and Enhancers;

Initiation of Transcription at Promoters;

Elongation and Termination of an RNA

Chain.

1.2 Transcription in Eukaryotes :

Eukaryotic RNA Polymerases; Eukaryotic

Promoters; Transcription of Protein

Coding Genes by RNA Polymerase;

Eukaryotic mRNA’s; Transcription of other

genes; Spliceosomes; RNA editing.

15

lectures

Unit II Gene expression- Translation

2.1 Nature of Genetic Code.Wobble Hypothesis.

2.2 Translation :Process of Protein Synthesis

(Initiation, Elongation, Translocation,

Termination); Post Translation

Modifications. Protein sorting.

15

lectures

Unit III Regulation of Gene expression 15

3.1 In Prokaryotes: In Bacteria: lac Operon of

E.coli; trp Operon of E.coli.

3.2 In Viruses :Lytic / Lysogenic Regulation

3.3 In Eukaryotes : Gene Silencing and Genomic

Imprinting; RNA Interference.

lectures

Ref:

Genes XI, 11th edition (2012), Benjamin Lewin,

Publisher - Jones and Barlett Inc. USA

Molecular Biology of the Gene, 6th Edition

(2008), James D. Watson, Pearson Education,

Inc. and Dorling Kindersley Publishing, Inc.

USA

Molecular Biology, 5th Edition (2011), Weaver

R., McGraw Hill Science. USA

Fundamentals of Molecular Biology, (2009), Pal

J.K. and Saroj Ghaskadbi, Oxford University

Press.

Molecular Biology: genes to proteins, 4th edition

(2011), Burton E Tropp Jones& Bartlett

Learning, USA

iGenetics A Molecular Approach 3rd Edition

Peter J. Russell.

Molecular Biotechnology-Principles and

Applications of Recombinant DNA Technology

3rd Edition Glick B.R., Pasternak J.J., Patten C.L

BUSBT306 Applied Microbiology 02

Unit I Air microbiology

1.1 Microorganisms present in air; Air microflora

(Transient nature of air flora) diseases caused

by air-borne microorganisms; Environmental

(physical) stress on microorganisms;

aeromicrobiological pathway - dispersion of

microoorganisms in air, bioaerosol (droplet

nuclei) and transport of bioaerosol, depo

sition of microorganisms; Air quality testing

methods.

1.2 Methods of air sampling and types of air

samplers

1.3 Diseases caused by air borne pathogens

1.4 Air sanitation.

15

lectures

Unit II Potable water and waste water microbiology

2.1 Aquatic environment and microorganisms -

nutrients and gases availability; microbial

community in aquatic environment; water

borne pathogens. Purification for drinking

water based on filtration, chlorination,

15

lectures

iodination treatment, UV treatment, reverse

osmosis their advantages & limitations

2.2 Water quality testing - sample collection

methods, water quality indicators - physical,

chemical and biological indicators,

bacteriological water analysis

2.3 Wastewater treatment: Steps involved in

wastewater treatment - (i) primary

(sedimentation, screening, coagulation,

flocculation, dilution, neutralization,

equalization); (ii) secondary (Activated

sludge treatment, Trickle filters, Anaerobic

filters ) and; (iii) tertiary (clarification,

disinfections, disposal of treated water)

Unit III Soil microbiology

3.1 Soil as a living system, Soil microbes and

plant growth - Rhizosphere, Soil

microorganisms and soil structure.

3.2 Biogeochemical cycling of elements -

carbon, nitrogen, sulphur, Nitrogen fixation-

symbiotic and non-symbiotic, Nitrification

and denitrification;

3.3 Soil microorganisms as bio -control agents;

15

lectures

Ref:

Environmental Biotechnology Allan Scragg

Oxford University press.

Environmental Biotechnology Indu shekar

Thakur IK International (Basic concepts and

applications)

Microbiology–6th Edition (2006), Pelczar M.J.,

Chan E.C.S., Krieg N.R., The McGraw Hill

Companies Inc. NY

Prescott‘s Microbiology, 8th edition (2010),

Joanne M Willey, Joanne Willey, Linda

Sherwood, Linda M Sherwood, Christopher J

Woolverton, Chris Woolverton, McGrawHil

Science Engineering, USA Food Microbiology-

Frazier

Credit

USBT307 RESEARCH METHODLOGY 02

Unit I

Introduction to Research Methodology and

Research Problem

1.1 Meaning of Research; Objectives of

Research; Motivation in Research; Types of

Research;

1.2 Research Approaches; Significance of

Research; Research Methods versus

Methodology; Research Process; Criteria of

Good Research; Problems Encountered by

Researchers in India;

1.3 What is a Research Problem? Selecting the

Problem; Necessity of Defining the

Problem; Technique Involved in Defining a

Problem

15

lectures

UNIT II

Research Design and Data Collection

2.1 Meaning of Research Design; Need for

Research Design; Features of a Good

Design; Important Concepts Relating to

Research Design; Different Research

Designs; Basic Principles of Experimental

Designs;

2.2 Developing a Research Plan-Collection of

Primary Data; Observation Method;

Interview Method; Collection of Data

through Questionnaires; Collection of Data

through Schedules; Other Methods of Data

Collection, Collection of Secondary Data,

Selection of Appropriate Method for Data

Collection, Case Study Method

15

lectures

UNIT III

Interpretation and Report Writing

3.1 Meaning of Interpretation, Why

Interpretation? Technique of Interpretation,

Precautions in

3.2 Interpretation, Significance of Report

Writing, Different Steps in Writing Report,

Layout of the Research Report, Types of

Reports.

3.3 Oral Presentation, Mechanics of Writing a

Research Report, Precautions for Writing

Research Reports.

15

lectures

Ref:

Research methodology- C.R. Kothari

PRACTICALS (SEM III)

BUSBTP301:Practicals based on Paper I and II (Credits:02)

1. Study of Absorption Spectra of Coloured Compounds (CuSO4, CoCl2,

KMnO4).

2. Verification of Beer-Lambert’s Law.

3. Extraction of Plasmid DNA and Separation by Agarose Gel Electrophoresis.

4. Determination of Purity of Plasmid DNA using UV Spectrophotometry.

5. Study of the Structure and Function of an Electron Microscope

6. Demonstration of Structure and Working of a Fluorescence Microscope

7. Electrophoresis of Proteins by SDS-PAGE.

8. Purification of any TWO Organic Compounds by Recrystallization Selecting

Suitable Solvent.

9. Organic Estimations: Acetone, Amide, Benzoic Acid.

10. Organic Preparations :

a) Acetylation of Primary Amine (Preparation of Acetanilide).

b) Base Catalysed Aldol Condensation (Synthesis of Dibenzalpropanone).

BUSBTP302:Practicals based on Paper III and IV (Credits:02)

1. Complement Fixation Test (CFT).

2. Passive Agglutination- RA Factor Test.

3. Immunoelectrophoresis.

4. ELISA (Kit-based) - HEPALISA.

5. DOT-ELISA.

6. Western Blotting - Demonstration.

7. Flow Cytometry - Lab Visit.

8. Study of Chromosomal Aberrations- Deletion, Duplication, Inversion,

Translocation and Syndromes- Trisomy 21 Trisomy 13 Trisomy 18,

Klinefelter, Turner and Cri-du-Chat.

9. Study of Polytene Chromosomes.

10. Mapping based on Tetrad Analysis and Three Point Cross.

11. Pedigree Analysis- Autosomal and Sex-Linked

BUSBTP303:Practical based on V and VI (Credits:02)

1. Qualitative and quantitative analysis of air flora by solid impaction methods

2. Contact slide method.

3. Isolation of nitrifying organism.

4. Study of Winogradsky’s column.

5. Routine analysis of potable water

6. Enrichment of Clostridia, Streptococci faecalis

7. Isolation of food spoilage causing organism (amylolytic, lipolytic and

proteolytic organisms)

8. Isolation of probiotic organism

SEMESTER IV

BUSBT401 Biochemistry 02

Unit I Carbohydrate Metabolism, ETS and

Energy Rich Compounds

1.1 Carbohydrate Metabolism :Glycolytic

Pathway and its Regulation,

Homolactic Fermentation; Alcoholic

Fermentation; Energetics of Fermentation;

1.2 Citric Acid Cycle and its Regulation;

1.3 Gluconeogenesis; Pentose Phosphate

Pathway; Glyoxalate Pathway; Reductive

TCA .(Sequence of Reactions, Regulation,

Energy Yield and Metabolic Disorders of

the above Pathways)

1.4 Electron Transport System:Electron

Transport and Oxidative Phosphorylation.

Inhibitors of ETS. Energy Rich Compounds :

ATP as Energy Currency, Structure of

ATP, Hydrolysis, Other Energy Rich

Compounds other than ATP like PEP,

Creatine Phosphate, etc.

15

lectures

Unit II Amino Acid Metabolism

2.1 Amino Acid Breakdown :Deamination,

Transamination, Urea Cycle, Breakdown of

Glucogenic and Ketogenic

2.2 Amino Acids.Amino Acids as Biosynthetic

Precursors : Biosynthesis of Epinephrine,

Dopamine, Serotonin, GABA, Histamine,

Glutathione.(Sequence of Reactions,

Regulation and Metabolic Disorders of the

above Pathways)

15

lectures

Unit III Lipids and Nucleotide metabolism

3.1 Lipid Metabolism : Mobilization, Transport of

Fatty Acids. Beta, Alpha and Omega

Oxidation of Saturated Fatty Acids;

3.2 Oxidation of Unsaturated Fatty Acids;

Oxidation of Odd Chain Fatty Acids.

3.3 Energy Yield, Ketone Body Breakdown to

Yield Energy.(Sequence of Reactions,

Regulation, Energy Yield and Metabolic

Disorders of the above Pathways)

3.4 Nucleotide Metabolism: Degradation of

Purines and Pyrimidines.

15

lectures

Ref:

Outlines of Biochemistry: 5th Edition, (2009),

Erice Conn & Paul Stumpf ; John Wiley and Sons,

USA

Principles of Biochemistry, 4th edition (1997),

Jeffory Zubey, McGraw-Hill College, USA

Lehninger , Principles of Biochemistry. 5th

Edition (2008), David Nelson & Michael Cox,

W.H. Freeman and company, NY.

Fundamentals of Biochemistry. 3rd Edition

(2008), Donald Voet & Judith Voet , John Wiley

and Sons, Inc. USA

Biochemistry: 7th Edition, (2012), Jeremy Berg,

Lubert Stryer, W.H.Freeman and company, NY

BUSBT402 Applied chemistry II 02

Unit I Sampling and separation techniques 1.1 Importance of Sampling and Sampling

Techniques.Types of Sampling - Random and

Non-Random.Sampling of Solids, Liquids and

Gases.

1.2 Separation Techniques :

Types of Separation Techniques -

Filtration, Zone Refining, Distillation,

Vacuum Distillation.Solvent Extraction -

Partition Coefficient and Distribution Ratio,

Extraction Efficiency, Separation Factor,

Role of Complexing Agents,Chelation, Ion

Pair Formation, Solvation, and Soxhlation.

1.3 Centrifugation - Basic Principles of

Sedimentation

15

lectures

Unit II Natural product chemistry

2.1 Natural Product Chemistry :Primary and

Secondary Metabolites.Classification of

Natural Products based on

BioSynthesis.Classification of Natural

Products based on Structure- Alkaloids,

Phenolics, Essential Oils and Steroids.

2.2 Structure Determination of Natural Products.

Commercial Synthesis of Natural Products.

2.3 Chromatographic Separation of Natural

Products : Gas Chromatography and its

Applications.Liquid Chromatography : HPLC

and its Applications.HPTLC for Separation

and Analysis of Natural Products.

15

lectures

Unit III Polymers and nanomaterials

3.1 Polymers :Introduction to Polymers.

Types of Polymers - Monomer, Polymer,

15

lectures

Homopolymer, Copolymer, Thermoplastics

and Thermosets, Addition and Condensation

Polymers (Examples and Uses)

3.2 Stereochemistry of Polymers.Biodegradable

Polymers.Nanomaterials :Introduction to

Nanomaterials. Forms of Nanomaterials :

Nanoparticles, Nanofilms and Nanotubes

3.3 Synthesis and Characterization of

Nanomaterials.Applications of Nanomaterials.

Ref:

Organic Chemistry, R.T. Morrison, R.N. Boyd

and S.K. Bhatacharjee, 7th Edition,Pearson

Education (2011).

Organic Chemistry, T.W.G. Solomon and C.B.

Fryhle, 9th Edition, John Wiley & Sons, (2008)

A guide to mechanism in Organic Chemistry, 6th

Edition, Peter Sykes, Pearson

Education

Fundamentals of Organic Chemistry , G. Marc

Loudon, 4th Edition Oxford

Organic Chemistry, L.G. Wade Jr and M.S. Singh,

6th Edition,2008 7. Organic

Chemistry, Paula Y. Bruice, Pearson Education,

2008

Organic Chemistry, J.G. Smith, 2nd Edition

Special Indian Edition, Tata 21 McGraw Hill

Organic Chemistry, S.H. Pine, McGraw Hill

Kogakusha Ltd

BUSBT403 Immunology 02

Unit I Infectious Disease

1.1 Host Parasite Relationship:

Normal Flora; Factors Affecting the Course of

Infection and Disease; Mechanisms of

Infection and Virulence Factors.

1.2 Infection: Patterns of Infection; Types of

Infections; Signs and Symptoms;

1.3 Epidemiology and Epidemiological Markers.

1.4 Diseases: Origin of Pathogens; Vectors;

1.5 Acquisition of Infection; Koch’s Postulates.

15

lectures

Unit II Medical Microbiology- causative organism

2.1 Characteristics ,Transmission, Course of

Infection, Lab Diagnosis, Management of

TB, Prevention and Control, Immuno and

Chemoprophylaxis

2.2 Skin : S. aureus.

15

lectures

2.3 Respiratory Tract Infections :M. tuberculosis,

S. pneumoniae

2.4 Urinary Tract Infections :E.coli

2.5 GI Tract Infections : Salmonella and Shigella

spps

2.6 Sexually Transmitted Diseases :Syphilis

2.7 Nosocomial Infections :P. aeruginosa

Unit III Introduction to Molecular Diagnostics :

3.1 Overview of Molecular Diagnostics; History

of Molecular Diagnostics; Molecular

Diagnostics in Post Genomic Era; Areas used

in Molecular Diagnostics; Future Prospects -

Commercialising Molecular Diagnostics,

Personalized Medicine, Theranostics.

3.2 Characterisation and analysis of Nucleic –

Acids and Proteins :Extraction, Isolation and

Detection of DNA, RNA and Proteins;

Restriction Endonucleases and Restriction

Enzyme Mapping. Hybridisation Techniques :

Southern, Northern, Western and FISH;

Markers, Probes and its Clinical Applications.

15

lectures

Ref:

Kuby immunology, Judy Owen , Jenni Punt ,

Sharon Stranford., 7th edition (2012), Freeman

and Co., NY

Immunology, 7th edition (2006), David Male,

Jonathan Brostoff, David Roth, Ivan Roitt,

Mosby, USA.

Mim’s Medical Microbiology 5th

edition

Microbiology by Prescott Harley and Klein 5th

edition Mc Graw Hill

Medical Microbiology Jawetz,E., Brooks,G.E,

Melnick,J.L., Butel,J.S Adelberg E. A18th edition

Medical Microbiology by Patrick Murray 5th

edition

Foundations In Microbiology by Talaro and

Talaro Third edition W.C Brown

BUSBT404 Environmental Biotechnology 02

Unit I Environmental Pollution

1.1 Sources of Pollution.

Air Pollution :Types; Sources; Classification

of Air Pollutants; Air Pollution Monitoring

and Control.

1.2 Water Pollution :Causes, Types and

Classification; Eutrophication; Assessment of

15

lectures

Water QualityPollutant Monitoring and

Control;

1.3 Soil and Solid Waste Pollution

:Characteristics of Wastes, Impacts of Solid

Waste on Health, Occupational Hazards

andControl. Soil Erosion :Concept, Causes

and Effects.

Unit II Global Environmental Problems and Issues

2.1 Green House Effect :Factors Responsible for

Green House Effect;

2.2 Green House Gases.Global Warming; Ozone

Depletion; Kyoto Protocol; UV Radiation;

Acid Rain.

15

lectures

Unit III Bioremediation

3.1 Concept of Bioremediation.Microorganisms

in Bioremediation, Mycoremediation and

Phytoremediation.

3.2 Bioremediation Technologies. Measuring

Bioremediation in the Field.

3.3 Bioaugmentation and Biostimulation.

Monitoring the Efficacy of Bioremediation.

15

lectures

Ref:

Environmental Biotechnology Allan Scragg

Oxford University press.

Environmental Biotechnology Indu shekar Thakur

IK International (Basic concepts and applications)

Biotechnology: Environmental Processes- Rehm

and Reed- Wiley

BUSBT405 Bioprocess technology 02

Unit I Microorganisms in industrial processes

1.1 Types of Microorganisms used in Industrial

Processes : Bacteria, Actinomycetes, Fungi

and Algae.

1.2 Screening and Maintenance of Strains:

1.3 Primary Screening and Secondary Screening;

1.4 Cultivation; Preservation of Industrially

Important Microbial Strains.

15

lectures

Unit II Fermentation processes and fermentor

2.1 Design of a fermentor :Stirred Tank

Fermentor- Basic Design; Parts of a Typical

Industrial Fermentor. Fermentation Media :

Components; Design and Optimization.

2.2 Sterilization : Sterilization of Fermentor and

Fermentation Media.

15

lectures

2.3 Process Parameters :pH, Temperature,

Aeration, Agitation, Foam, etc.

2.4 Types of Fermentation :Surface and

Submerged; Batch and Continuous, Aerobic

and Anaerobic.

2.5 Product Isolation and Purification.

Study of Representative Fermentation

Processes : Outline of Penicillin and Ethanol

Production by Fermentation along with a

flow-diagram.

Unit III Biostatistics

3.1 Theory and Problems based on- Coefficient

of Correlation and Regression Analysis;

3.2 Steps in Testing Statistical Hypothesis;

Parametric

3.3 Tests:- Z Test – Single Mean and Two Means,

t Test – Single Mean, Paired and Unpaired;

ChiSquare Test.

15

lectures

Ref:

Industrial Microbiology- A. H. Patel

Industrial Microbiology- L. E. Casida- John Wiley

& Sons

Introductory Biostatistics. 1st edition. (2003),

Chap T. Le. John Wiley, USA

Methods in Biostatistics- B. K. Mahajan –Jaypee

Brothers

BUSBT406 Applied Biotechnology 02

Unit I Plant tissue culture

1.1 Cell Theory, Concept of Cell Culture,

Cellular Totipotency, Organization of Plant

Tissue Culture Laboratory : Equipments and

Instruments. Aseptic Techniques: Washing of

Glassware, Media Sterilization,

1.2 Aseptic Workstation, Precautions to maintain

Aseptic Conditions.Culture Medium:

Nutritional requirements of t he explants,

PGR‘s and their in-vitro roles,

1.3 Media PreparationCallus Culture Technique:

Introduction, Principle and Protocols.

15

lectures

Unit II Basics of Animal Tissue Culture

2.1 Introduction. Cell Culture Techniques,

Equipment and Sterilization Methodology.

2.2 Introduction to Animal Cell

Cultures:Nutritional and Physiological:

Growth Factors and Growth Parameters.

15

lectures

General Metabolism and Growth Kinetics

2.3 Primary Cell Cultures : Establishment and

Maintenance of Primary Cell Cultures of

Adherent and Non-Adherent

2.4 Cell Lines with examples.Application of Cell

Cultures

Unit III Communication Skills

3.1 Introduction to Communication --Elements,

Definitions, Scope of Communication and

Communication as part of Science

Communication Elements --Verbal and

Non-Verbal Communications. Principles of

Effective Communication, Oral Presentations

3.2 Scientific Reading, Writing & Presentation

3.3 Scientific Writing Process of Scientific

Writing: Thinking, Planning, Rough Drafts

and Revising Contents.

3.4 Introduction to Scientific Reports and

Writings, Compilation of Experimental Data,

Communication Methods in Science,

Examples of Scientific and Unscientific

Writing.

3.5 Writing Papers, Reviews, Bibliography

3.6 Plagiarism--Introduction to Plagiarism ,

Examples of Plagiarism.

15

lectures

Ref:

Plant Biotechnology- K. G. Ramavat S.Chand

Publications

Culture of Animal cells- Ian Freshney -- John

Wiley & Sons

Principles and Practice of Animal Tissue culture-

Sudha Gangal - University Press

Research methodology- C.R. Kothari

BUSBT407 ENTERPRENEURSHIP DEVELOPMENT Credits

UNIT I

Introduction to Entrepreneurship Development

1.1 Concept of Entrepreneur; Entrepreneurship;

Need and Importance;

1.2 Factors Influencing Entrepreneurship;

Essentials of a Successful Entrepreneur

15

lectures

02

UNIT II

Setting-up of an Enterprise and Planning

2.1 Location of Enterprise; Real Estate and

Human Resource Planning, Financial

Planning; Role of Government and Financial

Institutions in Entrepreneurship

15

lectures

2.2 Development; Raising Money from Venture

Capitalists, Government Grants, Product

Selection and Ideas; Project Planning and

Formulation; Project Feasibility Assessment;

2.3 Regulatory Affairs, Corporate Laws,

Innovation, IPR generation and Protection,

2.4 Preparation of a Business Plan,

Characteristics and Importance of Planning;

UNIT III

Marketing, Sales, Advertising and International

Market research 3.1 Marketing Plan for an Entrepreneur;

Strategic Alliances, Advertising and Sales

Promotion;

3.2 Market Assessment, Need for International

3.3 Market Research, Domestic vs. International

3.4 Market Research, Cost and Methodology of

3.5 Market Research, Desk and Field Research

15

lectures

Ref:

Entrepreneurship – Kurup

Handbook of Entrepreneurship development-

Basotia and Sharma

PRACTICAL SEM IV

BUSBTP401: Paper I and II (Credits-02)

1. Determination of Lactate Dehydrogenase (LDH) Activity in Blood Serum.

2. Organ Function Tests: Liver (SGPT, SGOT); Kidney (Urea from Serum).

3. Estimation of Uric Acid and Creatinine in Urine.

4. Qualitative Detection of Ketone Body in Urine.

5. Separation of Binary (Solid-Solid) Mixture (Min 4 Compounds).

6. Identification of Organic Compound of Known Chemical Type (Min

4Compounds).

7. HPLC analysis and Interpretation of any one Secondary Metabolite from

Plants

8. HPTLC fingerprint analysis of any one Medicinally Important Plant.

9. Chemical and Biological Synthesis of Silver Nanoparticles and its

Characterisation by UV- VIS Spectrophotometer.

BUSBTP402: Paper III and IV (Credits-02)

1. Identification of S.aureus-Isolation, Catalase, Coagulase Test.

2. Identification of E.coli-Isolation, Sugar Fermentations, IMViC.

3. Identification of Salmonella- Isolation, Sugar Fermentations, TSI Slant.

4. Identification of Shigella- Isolation, Sugar Fermentations, TSI Slant.

5. Identification of Proteus- Isolation, Sugar Fermentations, IMViC.

6. Identification of Pseudomonas - Isolation, Urease test, Oxidase Test, TSI Slant.

7. Determining physiological parameters of water- pH; BOD; COD; Salinity,

chlorinity, Alkalinity, MPN, TDS; TSS, etc.

8. Bioremediation of Metal- isolation of heavy metal tolerant organisms from soil;

MIC and growth characteristics

9. Visit to STP / CETP and report on the visit

BUSBTP403: Paper V and VI (Credits-02)

1. Screening for an Antibiotic Producing Strain of Microorganism.

2. Lab Scale Production of Ethanol.

3. Purification of Ethanol from Broth Culture of Saccharomyces spp. by

Distillation.

4. Estimation of Penicillin by Chemical (Iodometric) Method.

5. Estimation of Penicillin by Biological (Bioassay) Method.

6. Estimation of Alcohol from Recovered Broth by Dichromate Method.

7. Laboratory Organization and Layout for Plant and Animal Tissue Culture

Laboratory

8. Preparation of Stock Solutions and Preparation of Media for PTC

9. Aseptic Transfer Technique, Surface Sterilization and Innoculation of explants

10. Study of Callus culture

11. Study of Media Preparation and Sterilization for ATC

12. Trypsinization of Tissue and Viability Count