DEPARTMENT OF BIOCHEMISTRY from the Department of Biochemistry ... Seminar Speakers ... undertake a...

DEPARTMENT OF BIOCHEMISTRY

Special Note

G reetings from the Department of Biochemistry……...

We have completed another successful academic year and we hope these pages give you some flavor of our activities over the past twelve months. We continue to wel-come new faculty in exciting areas of biochemistry. This year Dr. Mark Hall and Dr. Andy Tao began positions as As-sistant Professors. They bring critical expertise in protein mass spectrometry, a rapidly advancing field that is impact-ing investigations throughout the molecular life sciences. Dr. Tao was trained as an analytical chemist and worked in the emerging field of systems biology as a postdoctoral fel-low. His combined knowledge of organic chemistry and mass spectrometry applied to life sciences brings new op-portunities for interdisciplinary research. Dr. Hall began his career with training in biochemistry followed by postdoctoral experience in mass spectrometry. His research includes the detailed analysis of individual proteins and their post-translational modifications during the cell cycle. These fac-ulty members and their technical expertise are already ac-celerating research in the Department.

Our undergraduate enrollment continues to grow, giving us the opportunity to educate a new generation of biochemists. The rapid pace of advances in biochemistry requires keen analytical thought and the ability to integrate new information quickly. Our new requirement for independ-ent research experience will reinforce these skills in our undergraduate majors and help them sustain their careers over a lifetime. You will see in this year’s report that several students presented their results at scientific poster ses-sions on campus this year and we expect more participants in the future.

You may notice that a few stories in this issue go back to May of 2004. This is a result of our transition from a calendar year report (January through December) to an academic year report. We think this change better reflects the cycle of events during the school year and creates a summer mailing that is separated from our December holi-day letter.

Finally, I must thank all of you who have sent gifts to the Department over the past year. Your donations help build endowments for scholarships and lectureships, sup-port undergraduate student activities and recruit new stu-dents and faculty. As the federal and state budgets con-tinue to face revenue short falls, the challenges increase for Purdue and the Department of Biochemistry. I am deeply grateful for each gift that takes pressure off our gen-eral budget and allows me to assist the careers of our new faculty members and students. This year we have added the names of our donors over the past two years as public recognition of our gratitude. We also appreciate your sup-port by mentioning us to prospective students (both under-graduate and graduate), postdoctoral scientists, and faculty. Our future success depends on continuing to attract great people like you!

Jim Forney Department Head

Inside This Issue:Inside This Issue:Inside This Issue:

Faculty/Staff News ………………………………………. 2-5

Recognitions/Awards …………………………………… 5

Seminar Speakers ……………………………………….. 6

David W. Beach Memorial Lectureship ………….. 7

Graduate Student News ………………………………. 8-9

Undergraduate Student News ………………………. 10-12

Alumni Updates ……………………………………………. 12-13

Grants …………………………………………………………. 14

Donors…………………………………………………………. 15-16

Publications …………………………………………………. 17-18

At the very core of our cells are relics of an 'RNA World'. RNA catalysts such as the workhorse of cellular protein synthesis, the ribosome, and ribozymes, such as this self-splicing group I intron, both efficiently catalyze bio-chemical reactions and carry the genetic information necessary for their own replication. These molecules demonstrate that RNA, or a related molecule, could have been one of the first self-replicating molecules on Earth. This group I intron from bacteriophage Twort or-chestrates two successive phosphotransesterification reactions, allowing the RNA to excise itself from within a longer RNA, and to accurately splice the surrounding exons.

About the Cover:About the Cover:About the Cover: (from the Golden lab)

2004/2005 Annual Report Page 2

Mark Hall joined the department in June 2004. He grew up outside of Hartford, CT. He received his B.S. in Biology in 1994 at Trinity College in Hartford. In 1998, he received his Ph.D. from the Biology department at the University of North Carolina. Mark began his post-doctoral research at the National Institute of Environmental Health Sciences in the lab of Dr. Tom Kunkel where he studied mechanisms of DNA repair. In 2001, he moved back to UNC to undertake a second post-doctoral position to learn protein mass spectrometry and proteomics under the supervision of Dr. Christoph Borchers. It was there that he became involved in a pro-ject studying cell cycle regulation in yeast, a line of research he continues today.

Mark and his wife, Hana, have two sons, 5-year old Jakub and Brendan who was born shortly after their arrival in West Lafayette. Their family also includes a chocolate labrador re-triever, Chief, and a cat, Spivey. Before the joys of fatherhood, Mark spent a lot of time fishing, hiking, canoeing, writing music, playing basketball, and reading. Now, aside from changing dia-pers, he spends his sparse free time woodworking, gardening and sleeping.

Weiguo Andy Tao joined us in January 2005. Originally from China, Andy first enrolled at Kent State University in 1995. Following his advisor, Professor Roger Gilpin, he moved to Wright State University to complete his M.S. before starting his Ph.D. career in 1997 at Purdue with Professor R. Graham Cooks in the Department of Chemistry. His thesis work was on instrumentation and application of mass spectrometry for molecular recognition and enantiomeric determina-tion. After receiving his Ph.D. in December 2001, Andy joined the Institute for Systems Biology in Seattle, Washington

as a Damon Runyon Cancer Research Postdoctoral Fellow. While working for Professors Leroy Hood and Ruedi Aebersold, he focused his research on quantitative proteomics and phos-phorylation analysis.

Andy enjoyed the Drew Brees era and continues to be a big fan of Purdue sports, es-pecially football. His new laboratory space resides on the second floor of Hansen Life Science Building where his research program will be focused on the development of novel techniques for proteomic research and the application of proteomics to solve important biological issues. Andy is a full member of Bindley Bioscience Center in the Purdue Discovery Park. His training and affiliation with the Discovery Park will help him bridge technology with life science research in the department and across campus.

Faculty News New FacultyNew FacultyNew Faculty

Karl Brandt Mark Hall

Scott Briggs Mark Hermodson

Steve Broyles Ann Kirchmaier

Clint Chapple Joe Ogas

Harry Charbonneau Sandra Rossie

James Forney Andy Tao

Barbara Golden Henry Weiner

H. Lee Weith

Bernard Axelrod Forrest Quackenbush

Klaus Herrmann Victor Rodwell

Ki-Han Kim Ronald Somerville

Gunter Kohlhaw Roy Whistler

David Krogmann Howard Zalkin

The current faculty of the Department of Biochemistry includes the following Professors Emeriti:

Adjunct Associate Professor: Jon LeBowitz Adjunct Assistant Professor: Jill Hutchcroft

Current FacultyCurrent FacultyCurrent Faculty

2004/2005 Annual Report

Temporary AppointmentTemporary AppointmentTemporary Appointment Mark Hermodson, former department head and professor of biochemistry, is presently serving as Interim Associate Dean and Director of Agricultural Research Programs replacing Randy Woodson who was named Dean of the College of Agriculture in November 2004. After a national search, Dr. Sonny Ramaswamy from Kansas State University was named Associate Dean and will begin in January.

Mark Hermodson Randy Woodson

Research ReflectionResearch ReflectionResearch Reflection by Klaus Herrmann

S hortly after midnight on July 31, 2004 I came home extremely tired after a very

long day and a difficult drive through the rain: the happiest man you could imagine. Barbara Golden had invited me to the Ar-gonne National Laboratory’s Advanced Pho-ton Source to expose some of my DAHP syn-thase crystals. We started to work at 8:00 a.m. and I saw on “my” computer screen shortly before 8:00 p.m. the most wonderful black dots you can imagine in a sea of gray. It was a very emotional and the most excit-ing moment of my entire scientific career. I very much wish all of you hard-working bio-chemists a similar experience, at least once in your life. Thanks go to Barb, Elaine Chase for her expert help and constant encourage-ment in the preparation of the crystals, and the other members of the Golden Lab who accepted me cheerfully as a rookie some time ago.

“Down Under” “Down Under” “Down Under” Henry Weiner goes “Down Under” for a six-month sabbatical after receiving an Institute for Advanced Study Distinguished Fellow-ship Award from LaTrobe University in Melbourne, Australia. Hank took the time to work on manuscript writing and started a bioin-formatics project. In his free time, he and wife, Esther, enjoyed observ-ing beautiful birds such as parrots, cockatoos, magpies, ducks, and pen-guins, as well as numer-ous kangaroo and koalas in the wild and even a wombat or two.

Oh, the life of a roo!

NIHNIHNIH---NCRR CommitteeNCRR CommitteeNCRR Committee Victor Rodwell was appointed to a five-year term on the NIH-NCRR Committee. The com-mittee reviews proposals for Research Cen-ters in Minority Institutions (RCMI) and the IDeA Network of Biomedical Research Excel-lence (INBRE) that addresses biomedical re-search in states currently underrepresented for NIH funding.

Teaching in Mexico CityTeaching in Mexico CityTeaching in Mexico City

David Krogmann continues to teach and do hands-on research. In February he again traveled to the Universidad Nacional Automoma de Mexico, in Mexico City to teach a course to graduate students on “How to Write a Scientific Paper in English.”

Cancer ResearchCancer ResearchCancer Research

E ach year hundreds of thousands of Americans die of one of the more than 90 diseases collectively known as cancer. Harry Charbonneau, Ann Kirchmaier, Scott Briggs, Jill Hutchcroft, Sandra Rossie, Barbara Golden, Mark

Hermodson and Henry Weiner were featured in the fall 2004 issue of Purdue Agricultures Magazine for their involve-ment in cancer research through the Purdue Cancer Center. The Center is among 61 National Cancer Institute-designated research groups around the nation established as a provision of the 1971 National Cancer Act. Our eight faculty are among the more than 70 researchers throughout the university involved in the Center’s three scientific programs: Cell Growth and Differentiation; Structural Biology; and Experimental Therapeutics and Diagnostics.


CelebrationCelebrationCelebration

T he department helped Drs. Herrmann, Rodwell and Somerville commemorate

their retirement in a September 2004 cele-bration. Combined they contributed 111 years of service to the department! We were honored to have been joined by so many alumni from across the country to extend their goodwill.

The department wishes each of our retirees many wonderful years ahead!

Klaus Herrmann Victor Rodwell Ronald Somerville

Victor Rodwell and Bryant Darnay Ph.D. ‘92 (Rodwell)

Cytokine Research Section University of Texas

M. D. Anderson Cancer Center

Doug Johnson Ph.D. ‘83 (Somerville)

Hong Smith M.S. ’98 (Somerville)

Gregg Bogosian Ph.D. ‘83 (Somerville)

Jisong Cui Ph.D. ‘92 (Somerville)

and Gaochao Zhou Ph.D. ‘91 (Zalkin)

G. Robert Greenberg Ron Somerville’s mentor

Lian-Mei (Madge) Yang Graham Ph.D. ‘73 (Somerville) and

Terry Graham Ph.D. ‘75 (Whistler)

Ronald Somerville’s family: Ken, Joyce, Tracy, Victoria, and Gordon

Michael Keith Ph.D. ‘81 (Rodwell)

Department of Chemistry Cal. Poly.

Victor Rodwell’s structure display

Faculty RetireesFaculty RetireesFaculty Retirees


C onnie Bonham, Instrument Specialist (20 years), Karyn Rodkey, Manager of Research Services (15

years), and Penny Pava, Secretary (10 years) were rec-ognized for their years of service to Purdue and the De-partment. Their hard work and dedication is greatly ap-preciated.

Connie Bonham Penny Pava

Recognitions and Awards

T his award is given each year to mid-career graduates of Purdue’s College of

Agriculture who have made significant con-tributions to their profession or to society. Curtis Hannah of Gainesville, Florida was one of nine recipients honored with this award in 2004.

Dr. Hannah received his B.S. from the Depart-ment of Biochemistry in 1967 and his M.S. from the Department of Botany and Plant Pathology in 1969. In 1973, he received his Ph.D. in Genetics from the Univer-sity of Wisconsin. He advanced from Assistant, Associ-ate and then to Professor in the Vegetable Crops De-partment, presently the Horticultural Sciences Depart-ment at the University of Florida.

In 1987, Professor Hannah became founding co-director of the Interdisciplinary Center for Biotechnology Research at the University of Florida where he was named Research Foundation Professor in 1999. He is presently with the Plant Molecular and Cellular Biology at the Uni-versity of Florida.

During his visit, Dr. Hannah presented a seminar entitled, “Modified Sh2 Increases Wheat and Rice Yield 38% and 23%.”

L. Curtis Hannah

Distinguished Agricultural Alumni Distinguished Agricultural Alumni Distinguished Agricultural Alumni

Johanna Zalkin and Howard Zalkin

Claudia Kent and Jack Dixon

Esther and Henry Weiner, Karl Brandt, and Ourania Andrisani

“Reception”“Reception”“Reception”

J ack Dixon was presented an honorary doctorate degree at the May 2004 com-

mencement ceremony. Jack was a member of Purdue’s Biochemistry faculty from 1973-1991. In 1986, he was named Distin-guished Professor of Biochemistry. On his visit he was accompanied by his wife, Dr. Claudia Kent, who was a faculty member in the department from 1975-1991.

Jack received his B.S. from the University of California, Los Angeles, his Ph.D. from the University of California, Santa Barbara and his Postdoctorate from the University of California, San Diego. Jack is currently Dean for Scientific Affairs at the University of California, San Diego and resides in La Jolla, California.

Jack Dixon

Former Purdue Faculty Former Purdue Faculty Former Purdue Faculty Receives Honorary Doctorate Receives Honorary Doctorate Receives Honorary Doctorate

T he College of Agriculture annually rec-ognizes administrative or professional

staff who has exhibited excellent perform-ance. We are proud to announce the pro-motion of Dr. Yinglin Bai, Research Associ-ate, to rank five. Yinglin has been with the University since 1996 and is currently working in Dr. Tao’s lab.

Years of Service AppreciationYears of Service AppreciationYears of Service Appreciation A/P Staff Rank Advancement A/P Staff Rank Advancement A/P Staff Rank Advancement

Karyn Rodkey


Seminar Series

T his year the Department of Biochemistry initiated a weekly seminar series and hosted many guest speakers both from within Purdue and from other institutions (pictured below). The series was a success and we look forward to hosting

another quality series in the upcoming year.

Dan Liebler Department of Biochemistry

Vanderbilt University School of Medicine

“Toxicoproteomics: Proteome damage and adaptation in chemical toxicity”

Craig Peterson Pharmacology

University of Massachusetts Medical School

“Chromatin remodeling machines: Regulators of DNA repair and transcription”

Andy Belmont Department of Cell and Structural Biology

University of Illinois

“Large-scale chromatin structure and dynamics”

Ronen Marmorstein Department of Biological Chemistry

The Wistar Institute

“Structure and chemistry of enzymes that mediate acetyl-transfer in gene regulation”

Steve Broyles Department of Biochemistry

Purdue University

“Poxvirus gene regulation and pathogenesis; sharks swimming in the cytoplasm”

Scott Briggs Department of Biochemistry

Purdue University

“Histone methylation SETing a stage for chromatin function”

Clint Chapple Department of Biochemistry

Purdue University

“Plant metabolism: the last 400 million years”

Rich Gibbs Department of Medicinal Chemistry

and Molecular Pharmacology Purdue University

“Combinatorial Modulation of Protein Prenylation”

Barb Golden Department of Biochemistry

Purdue University

“Crystal Structure of a group I ribozyme-product complex”

Dave Krogmann Department of Biochemistry

Purdue University

“The structure of the water soluble orange carotenoid protein and its possible functions”

Joe Ogas Department of Biochemistry

Purdue University

“PKL and the self-basting beets”

Carol Post Department of Department of Medicinal Chemistry

and Molecular Pharmacology Purdue University

“Protein NMR and dynamics”

Dan Szymanski Department of Agronomy

Purdue University

“Subunits of the WAVE and ARP2/3 complexes control leaf epidermal morphogenesis”

Irwin Tessman Department of Biological Sciences

Purdue University

Work in progress: “A look at some outstanding problems”

Jon Wilker Department of Chemistry

Purdue University

“Biomaterials at the beach: Metal-protein interactions in mussel & barnacle adhesive”


David W. Beach Memorial Lectureship

I nspired by their son’s enthusiasm for science, David and wife, Doris, chose to share their good fortune by establishing this Lectureship in 1981. Michael Beach received his Ph.D. in 1987 under the guidance of Dr. Victor Rodwell. The long-

term support of the David W. Beach Memorial Lectureship in Biochemistry is intended to promote intellectual curiosity, a commitment to excellence, and an appreciation of science in all those involved.

Dr. Mark Stitt, from the Max-Planck Institute for Molecular Plant Physiology, presented the 11th Lecture Series in February 2005. Dr. Stitt presented two separate seminars, “Running out of control: approaches to study the control of

metabolism” and “(Dys)Functional genomics: a comprehensive insight into system function.” In the first lecture, Dr. Stitt surveyed methods that are taken in ‘classical’ biochemical approaches to identify and characterize the steps that are responsible for the regulation of metabolic flux. In the second lecture, he discussed how a multilevel phenotype platform allows dissection of com-plex biological responses like the response to low carbon and nutrients.

Dr. Stitt has been awarded the Presidents Medal of the Society for Experimental Botany in England and was identified by ISI as a Highly Cited Researcher based upon his publications. Over-all, Dr. Stitt has over 200 peer-reviewed publications to his credit. Mark Stitt

Distinguished Lecture Series DinnerDistinguished Lecture Series DinnerDistinguished Lecture Series Dinner

Chris and Michelle Drennan (Broyles lab) with Sadie LaBauve and Dr. Ian Fingerman

(Briggs lab)

Dr. and Mrs. Rodwell with Dr. Jill Hutchcroft

Dr. Mark Hermodson (far right) with our guests, Dr. David Salt (Horticulture) and Dr. Jiri

Adamec (Discovery Park)

Two of our newest faculty Drs. Mark Hall and Ann Kirchmaier

Drs. Gunter Kohlhaw and Klaus Herrmann discuss the health benefits

of resveratrol. Drs. Mark Stitt and Clint Chapple


Anindya Chatterjee Michelle Drennan Dah-Eun (Mary) Jeong Juan Martinez Efrain Sanchez

Graduate Student News

New Ph.D. StudentsNew Ph.D. StudentsNew Ph.D. Students

FellowshipsFellowshipsFellowships

G raduate School Fellowship Incentive Grants were awarded to two Biochemistry graduate students. James Henderson (Ogas lab) was awarded an Incentive Grant for his application to the

EPA Science to Achieve Results (STAR) Fellowship. James also received the Bilsland Dissertation Fellowship award provided to support outstanding Ph.D. students in their final year of writing. Efrain Sanchez (Rossie lab) received an Incentive Grant for his application to the Ford Foundation Pre-Doctoral Fellowship. James Henderson

T imothy Holzer was the recipient of the 2005 A. K. Balls Award given annually to a graduate student who, as a young scientist, exhibits unusual potential for significant contributions to biochemical research and who is approaching

completion of their pre-doctoral program. Tim entered the program in 1999 and conducted research on gene expression in Leishmania, a protozoan parasite. His recent research included whole-genome microarray analysis using the sequenced genome of one Leishmania species to successfully examine mRNA abundance in a closely related species. This approach has special significance in parasitology due to the large number of closely related species that cause different clinical symptoms. In addition to his research, Tim is actively involved in the University community, meeting with prospective faculty, organizing graduate student events, and serving as the Biochemistry Senator for the Purdue Graduate Student Government.

Hickory Stick AwardHickory Stick AwardHickory Stick Award

J ui-Hui (Rae) Chen is the 2005 recipient of the Hickory Stick Award given annually for outstanding performance as a graduate teaching assistant. The recipient is recognized for the qualities which

are essential for future success in teaching and research. Rae was the teaching assistant for two of the Spring BCHM 309 labs. Her subject knowledge, dynamics, strong mentoring atmosphere, boundless energy and commitment to the students earned her this award.

Timothy Holzer

Jui-Hui (Rae) Chen

T he department continues to recruit excellent students to our graduate program and welcomed five new Ph.D. students in August. Anindya Chattterjee received his M.S. in 2002 from Manipal Academy of Higher Education in India;

Michelle Drennan came to us after receiving her B.S. in 2004 from Austin State College in Texas; Dah-Eun (Mary) Jeong received her B.S. in 2003 from California State University in Hayward; Juan Martinez joined us from Arkansas State University in Jonesboro where he received his B.S. in 2004; and Efrain Sanchez received his B.S. in 2004 from University of Puerto Rico in Humacao.

A. K. Balls AwardA. K. Balls AwardA. K. Balls Award


Degrees Awarded ~ ~ Summer and Fall 2004Degrees Awarded ~ ~ Summer and Fall 2004Degrees Awarded ~ ~ Summer and Fall 2004

Degrees Awarded ~ ~ Spring 2005Degrees Awarded ~ ~ Spring 2005Degrees Awarded ~ ~ Spring 2005

Hui-Chun Li PBP, Ph.D.

“Functional characterization of the PICKLE chromatin remodeling factor in

Arabidopsis thaliana”

Joe Ogas

Benjamin Linger BMB, Ph.D.

“Sindbis virus nucleocapsid core assembly: Char-acterization of the initiation complex that pro-

motes genome packaging”

Commencement Reception Great Success!Commencement Reception Great Success!Commencement Reception Great Success!

T he Spring 2005 College of Agricul-ture commencement reception was

a great success. The reception was held in the Purdue Memorial Union’s ballrooms with more than 900 people in attendance. The Department of Bio-chemistry was represented by Paula Flores (Secretary), Scott Briggs (Assistant Professor), Karl Brandt (Professor), and Mark Hermodson (Professor).

Poster designed by Paula Flores Purdue Memorial Union

Degrees Awarded ~ ~ Spring 2004Degrees Awarded ~ ~ Spring 2004Degrees Awarded ~ ~ Spring 2004

Autumn Sutherlin BMB, M.S.

(Victor Rodwell)

“The Mevalonate pathway of Isopentenyl Pyrophosphate Biosynthesis in Enterococcus faecalis: A potential target

for antimicrobial agents” Victor Rodwell

Stephanie Doun BCHM, M.S.

“Enterococcus faecalis Phosphomevalonate

Kinase”

Victor Rodwell

Yan Liu BCHM, Ph.D.

“Nbp1 is a Novel Component of the Saccharomyces cere-visiae Spindle Pole Body and

a Target of the Cdc 14 Phosphatase”

Harry Charbonneau

Jaewook Oh BMB, Ph.D.

“The localization and the function of cellular transcription factors in

Vaccinia virus”

Steve Broyles

Barbara Golden


Undergraduate Student News

BCHM 490 Poster PresentationBCHM 490 Poster PresentationBCHM 490 Poster Presentation

T he new departmental undergraduate capstone experience requires independ-ent research and presentation of the results at a department poster session.

Eight students in the BCHM 490 class participated in this year’s poster presenta-tion. Kate Estell, Thomas Evans, Albert Felten, Yoshiaki Hagiwara, Stephanie Mowery, Megan O’Shaughnessey, Andy Tancos and Bradley Wilson described their research to faculty, graduate students and staff.

T hree students from the Biochemistry Department were honored at the College of Agriculture Spring

Awards Reception. Outstanding students were Brenton Goodman (Freshman), Kyle Mohler (Sophomore), and Theresa Baltz (Junior). Theresa was also a recipient of the 400 Club award. Jacob Adler, Brenton Goodman, and Scott Secrist were also given recognition by the 400 Club for their outstanding accomplishments.

A group of Purdue students partici-pated in the State Science and En-

gineering Fair in Indianapolis where they judged exhibits designed by middle and high school students. This was a good experience for Kyle Mohler, a stu-dent in Biochemistry, along with other students who attended.

Student Participates in FairStudent Participates in FairStudent Participates in Fair

Research and Poster Symposium AwardResearch and Poster Symposium AwardResearch and Poster Symposium Award

Holly Courtney (Joe Ogas lab)

Kate Estell (Mark Hall lab)

Yoshiaki Hagiwara (Joe Ogas lab)

Megan O’Shaughnessey (Joe Ogas lab)

S ummer Wilson was one of sixteen recipients of this year’s Undergraduate Research and Poster Symposium Award. The Purdue College of Science, Purdue Science Student Council and the College of Agriculture hosted the 2005

Symposium to recognize those undergraduate students who are performing research and to raise awareness of and answer questions concerning undergraduate research.

Holly Courtney , Kate Estell, Yoshiaki Hagiwara, and Megan O’Shaughnessey also presented posters on their undergraduate research.

Student Enrollment(Undergraduates)

42 41 4137

53

69

0

10

20

30

40

50

60

70

80

'99 '00 '01 '02 '03 '04

Year of Enrollment (Fall)N

umbe

r of S

tude

nt

Enrollment Increases!Enrollment Increases!Enrollment Increases! 2005 Spring Awards2005 Spring Awards2005 Spring Awards

Kyle Mohler


Undergraduate Scholarships AwardedUndergraduate Scholarships AwardedUndergraduate Scholarships Awarded

Scholarship Award of ExcellenceScholarship Award of Excellence

Shannon Borneman Brenton Goodman

Kimberly Han Jeremy Smurr

Anthony Snyder Christopher Sprunger

Dustin White Abby Lillpop

Lewis Runkle ScholarshipLewis Runkle Scholarship

Kenneth Clark Joshua Cark

Mauri Williamson Scholarship for Excellence in AgricultureMauri Williamson Scholarship for Excellence in Agriculture

Amanda Burns

J. Kelly O’Neall and Margaret Ritchey O’Neall Memorial ScholarshipJ. Kelly O’Neall and Margaret Ritchey O’Neall Memorial Scholarship

Michael Hanlon

John W. Ryan Scholarship Honoring Nancy and Arthur HansenJohn W. Ryan Scholarship Honoring Nancy and Arthur Hansen

John Kronberg

Floyd E. and Nellie P. Elliott ScholarshipFloyd E. and Nellie P. Elliott Scholarship

Francis Lagoe

Edwin T. Mertz Memorial ScholarshipEdwin T. Mertz Memorial ScholarshipEdwin T. Mertz Memorial Scholarship

R onald and Carolyn Chance have provided substantial gifts to the Edwin T. Mertz Memorial Scholarship for under-graduate students in Biochemistry. Dr. Ronald E. Chance is a distinguished researcher at Eli Lilly where he has

long been involved in insulin research. Edwin T. Mertz was Professor of Biochemistry at Purdue from 1946-1976. Pro-fessor Mertz was recognized globally for his co-discovery of high-lysine corn. Dr. Mertz received honorary degrees from Purdue and the University of Montana and was elected to the National Academy of Sciences in 1975.

Biochemistry Undergraduate Students are invited to apply for the award by completing an application and writing an essay describing their reasons and goals for studying biochemistry. The 2005-2006 winners were Melanie Ivanic, Indianapo-lis (a continuation of her award of last year) and Theresa Baltz, West Lafayette.

Theresa Baltz Melanie Ivanic

O ver the past year, the Biochemistry Club met bi-weekly to listen to speakers and discuss club activities such as Elementary Outreach and Spring Fest. The speakers provided our

students with insights into the various fields they may choose to pursue after graduation, and included Purdue faculty and graduate students, biochemists in industry, and physicians.

One of the club’s most ambitious and important endeavors continues to be elemen-tary outreach. Members visit students in grades 3-8 in the greater Lafayette area to teach simple biochemistry concepts. They work with teachers to construct demonstrations that relate to topics currently being discussed in the classroom. Some projects taught this past year include Reebops (a simple genetics experiment), making silly putty to demonstrate poly-

mers, and viewing a multitude of specimens with microscopes. The Outreach program also traveled to Durgan Elementary School to help with National Chemistry Week.

Along with Elementary Outreach, Spring Fest is a great way to inform the community about biochemistry and the club. During this year’s event (held April 16 & 17), the club booth displayed posters explaining concepts behind the value and utility of genetically modified organisms.

Sophomore ScholarshipSophomore Scholarship

Michael Hanlon Kyle Mohler

Students Reach OutStudents Reach OutStudents Reach Out


Undergraduate Class of 2005Undergraduate Class of 2005Undergraduate Class of 2005

Albert E. Felten, Kate A. Estell, Thomas D. Evans, and Bradley D. Wilson

Alumni Updates

1950s

Melvin E. Gleiter - M.S. 1956/Ph.D. 1958 (Herbert Parker). Melvin served as Professor and Head of the University of Wisconsin’s Chemistry Department in Eau Claire, Eau Claire, Wisconsin until his retirement. He married Beverly Wright on April 17, 2004.

1960s Robert E. Gramera - M.S. 1961/Ph.D. 1964 (Roy Whistler). Bob resides in Durango, Colorado.

Charles D. Rowe - M.S. 1964 (Ned Draudt). Charles is em-ployed as Technical Director of StanChem, Inc.

George Cruzan - Ph.D. 1969 (Forrest Quackenbush). Since 1995, George has provided toxicology consulting services under the name of ToxWorks. Projects include toxicity as-sessments, study design and management, regulatory sub-missions and comments on proposed regulations or govern-ment evaluations of chemicals. George is presently living in Bridgeton, New Jersey.

1970s Robert Parson - B.S. 1972. Robert has many fond memories of his time in the Biochemistry department at Purdue. He is Senior Staff Biostatistician at Beckman Coulter, Incorporated in San Diego, California. He served on the development team for FDA approval of the PSA (Prostate Specific Antigen) which is used to detect prostate cancer. Currently at the University of California, San Diego, Robert is completing a Masters in Advanced Studies in the leadership of healthcare organiza-tions.

A. William (Bill) Tank - Ph.D. 1976 (Henry Weiner). Bill is pres-ently Paul Stark Professor of Pharmacology and Professor and Chair of Pharmacology and Physiology at the University of

(

~~~~ In Memory of In Memory of In Memory of ~~~~

H enry Nelson Wood, Ph.D. 1955 (major professor, A.K.

Balls) passed away in July 2004 after an extended battle with renal disease. After graduation, Dr. Wood was on the faculty of Rockefeller University in New York City for 35 years. While there, he pursued can-cer research utilizing studies of the crown gall disease in plants to ad-vance knowledge of the substances and mechanisms involved in the regulation of cell growth. Among other achievements, he discovered a class of regulators in higher plants, and isolated six cell-division-promoting factors from crown gall tumor cells. During the course of his career he authored or co-authored over 40 scientific publications. Dr. Wood became emeritus professor at Rockefeller University in 1990 and retired with his wife to Harpers Ferry, West Virginia.

Department of Biochemistry

(Continued on page 13)


Rochester School of Medicine and Dentistry. His research in-terests are in the general fields of gene regulation and signal transduction in the nervous system. Bill’s laboratory focuses on the gene encoding tyrosine hydroxylase (TH), the enzyme catalyzing the first step in catecholamine biosynthesis where they hope to gain insight into changes in neuronal gene ex-pression that are responsible for Parkinson’s disease that occur following stress and that mediate physical dependence and addiction to drugs of abuse.

1980s

James M. Groarke - Ph.D. 1984 (Mark Hermodson). James is presently employed with NIBRI (Novartis Institutes for Biomedi-cal Research, Incorporated) located in Cambridge, Maryland.

Scott Pattison - B.S. 1987 (Klaus Herrmann). Scott stated his undergraduate experience at Purdue was invaluable. For the past two years, he has served as the Director of Molecular Biology at Kemp Biotechnologies, Inc. in Frederick, Maryland. After graduating from Purdue, Scott later earned an M.S. from the Biochemistry, Molecular Biology and Cell Biology department at Northwestern University. He worked for two years at Boehringer Mannheim’s Diagnostics Divison (now Roche) in Indianapolis, Indiana. Scott completed a Ph.D. in the Microbiology and Immunology department at the Indiana University Medical Center and a two year postdoctoral fellow-ship at St. Jude Children’s Research Hospital in Memphis, Tennessee from 1996-1998. Following his postdoctoral, Scott was hired as a Senior Scientist at Genetic Therapy, Incorporated where he worked in their Adenoviral Vectors Program for four years.

Tuajuanda Jordan - Ph.D. 1989 (Victor Rodwell). Tuajuanda was promoted to Associate Vice President for Academic Af-fairs at Xavier University of Louisiana.

1990s

Sharon J. (McLaughlin) Sweitzer - Ph.D. 1993 (Jack Dixon) and Tom Sweitzer - Ph.D. 1993 (Claudia Kent). Sharon and Tom are both employees of GlaxoSmithKline Pharmaceuti-cals in King of Prussia, Pennsylvania. Sharon runs a gene expression laboratory where she sets up recombinant protein expression in CHO, baculovirus, E. coli, etc. for protein supply of high throughput screening, crystallography efforts, anti-gens for antibody generation, and a variety of other special-ized needs. Tom runs a cell culture scale-up lab in the same department (Gene Expression and Protein Biochemistry). His lab focuses on large scale production (up to 100L reactors or a few hundred roller bottles) of recombinant proteins in baculovirus, drosophila, or a variety of mammalian cell sys-tems. Tom is continually finding his degree at Purdue pre-pared him well for his career. He informed us that he doesn’t find a comparable background in molecular biology, recombi-nant protein expression, purification, enzymology or general biochemistry in many of his peers.

Yi Wang - Ph.D. 1993 (Henry Weiner) and his wife, Siqi Lin – Ph.D. 1994 (Steve Broyles). Siqi worked for GlaxoSmithKline Pharmaceuticals in King of Prussia, Pennsylvania for two and a half years where she managed the cell culture scale-up facility for the high throughput screening group. The couple presently reside in Connecticut.

Kenneth Bischoff - Ph.D. 1995 (Victor Rodwell). Ken is pres-ently working at the USDA-ARS facility in Peoria, Illinois in the field of biofuel research.

Peter L. Nagy - Ph.D. 1995 (Howard Zalkin). Peter presently holds a tenure research position, which includes 20% clinical duty as an anatomic/molecular genetic pathologist, at the University of Iowa in the Pathology department. This involves mostly genetic testing of patients with various cancers and cancer syndromes. Peter resides in Iowa City, Iowa.

Gregory Taylor - Ph.D. 1998 (Harry Charbonneau). Greg has accepted a position as Assistant Professor in the Department of Biochemistry and Molecular Biology at the University of Nebraska Medical Center, Omaha, Nebraska. He started July 1, 2005.

Alumni Updates (cont’d)

A nthony L. Schilmiller was the recipient of the Anton Lang Memorial Graduate

Student Award for his Ph.D. research on allele oxide synthase with Dr. Gregg Howe at the MSU-DOE Plant Research Lab. The title of his disserta-tion was Biochemical and Physiological Stud-ies on Plant Oxylipins. Tony received his B.S. from Purdue in 2000.

We want to hear from our alumni. Please take a moment to tell us what is going on in your life……

Comments, suggestions and updates can be sent to [email protected]. Include your name, address, email address, degree, major, and year of graduation. You can also up-date by using our website.

(www.biochem.purdue.edu)

Graduate Student Award

Anthony L. Schilmiller


Grants

Scott Briggs, Indiana Elks Charities (Co-PI), $25,000, 9/1/03 through 8/31/05, “Cancer Research.”

Scott Briggs, American Cancer Society-Cancer Center, $20,000, 2/1/04 through 12/31/04, “The Role of MMSET in Cellular Transformation.”

Scott Briggs, Sidney Kimmel Foundation for Cancer Research, $200,000, 7/1/04 through 6/30/06, “Functional Characteriza-tion of the Multiple Myeloma Set Domain Protein (MMSET).”

Scott Briggs, PU/IU Collaborative Biomedical Research (Co-PI), $25,000, 1/1/05 through 12/31/05, “Functional Analysis of a Novel Set Domain Protein in Histone Methylation, Gene Expres-sion, and Oncogenesis.”

Steve Broyles, NIH-Discovery Park, $142,255, 9/30/02 through 8/31/07, “Micromechanical Sensors for Virus Detection.”

Steve Broyles, National Institutes of Health, $186,616, 7/1/03 through 12/31/04, “Training in Biochemistry and Molecular Biology.”

Clint Chapple, National Science Foundation, $467,601, 4/1/01 through 3/31/05, “Identification and Characterization of the Last Unknown Catalyst in Lignin Biosynthesis.”

Clint Chapple, National Science Foundation, $500,811, 4/1/01 through 3/31/05, “Serine Carboxypeptidase-Like Proteins: Misunderstood Catalysis in Plant Metabolism.”

Clint Chapple, National Science Foundation, $450,000, 4/1/05 through 3/31/08, “Functional Analysis of Phenylpropanoid Cy-tochrome P450-Dependent Monooxygenases.”

Clint Chapple, National Science Foundation, $1,054,380, 9/1/04 through 8/31/07, “Acquisition of Metabolic Profiling Instrumentation at Purdue University.”

Clint Chapple, Department of Energy, $360,000, 4/1/03 through 3/31/06, “Secondary Metabolism in Arabidopsis.”

Harry Charbonneau, National Institutes of Health, $1,219,741, 9/30/99 through 9/30/05, “Function and Regulation of the CDC14 Protein Phosphatase.”

James Forney, National Science Foundation, $646,472, 8/1/01 through 7/31/05, “DNA Rearrangements and Macronuclear Development in Cilated Protozoa.”

James Forney, National Institutes of Health, $1,408,925, 9/1/00 through 5/31/06, “Regulation of Flagellum and PFR Elaboration in Leishmania.”

Barbara Golden, National Aeronautics and Space Administra-tion, $424,250, 9/1/01 through 11/30/05, “Engineering a Ribozyme for Diffraction Properties.”

Barbara Golden, University of California-SF (PEW), $255,058, 7/1/00 through 6/30/05, “Structural Biology of Catalytic RNAs.”

Mark Hall, American Cancer Society-Cancer Center, $20,000, 12/1/04 through 11/30/05, “American Cancer Society Institu-tional Research Grant.”

Mark Hermodson, U.S. Department of Agriculture, $508,245, 2/1/01 through 12/31/05, “Biochemistry of Soybean Protein and Oil II.”

Mark Hermodson, U.S. Department of Agriculture, $74,086, 11/10/03 through 11/9/04, “Task Order for Biochemistry Por-tion of Task Order T-4-3602-102.”

Mark Hermodson, U.S. Department of Agriculture, $28,750, 10/1/04 through 9/30/05, “Task Order for FY 04-05.”

Mark Hermodson, IU School of Medicine (Co-PI), $107,132, 7/1/04 through 6/30/05, “Lafayette Center for Medical Educa-tion.”

Mark Hermodson, The Protein Society, $546,379, 1/1/98 through 12/31/05, “Protein Science.”

Ann Kirchmaier, Sidney Kimmel Foundation for Cancer Re-search, $200,300, 7/1/03 through 6/30/05, “Epigenetic Regu-lation of Epstein-Barr Viral Gene Expression.”

Joe Ogas, National Institutes of Health, $1,261,428, 4/1/00 through 3/31/06, “Analysis of Role of CHD Proteins in Arabi-dopsis thaliana.”

Joe Ogas, Lemelson Foundation/NCIIA, $17,700, 8/1/04 through 1/31/06, “Pickle Technology.”

Victor Rodwell, American Heart Association, $200,418, 1/1/01 through 12/31/04, “Bacterial HMG-COA Reductase: Active Architecture and Model for New Antibiotics.”

Sandra Rossie, National Institutes of Health, $1,570,645, 6/1/02 through 5/31/07, “Role and Regulation of Protein Phosphatase 5 in Brain.”

Henry Weiner, National Institutes of Health, $1,430,941, 6/15/04 through 5/31/08, “Enzymology/Molecular Biology of Aldehyde Dehydrogenase.”


Dr. G. Steven Acres Dr. Patricia C. Allen Dr. Andrew J. Alpert Mrs. Nona M. Alyea Mr. David L. Andrews Dr. Arthur I. Aronson Dr. Melissa A. Ashlock Dr. Bernard Axelrod Dr. Eric T. Baldwin Mr. John W. Battles Mrs. Beth M. Beeson Ms. Naomi O. Blay Dr. Kenneth M. Bischoff Dr. Scott D. Briggs Dr. Herbert C. Brown Dr. Robert M. Bruce Mr. Michael E. Burch Dr. Donald A. Burns Dr. Carl A. Burtis Jr. Dr. R. Lance Burton Mrs. Tishu Cai Mr. Charles S. Campbell Dr. Don M. Carlson Dr. David F. Carmichael Dr. and Mrs. Ronald E. Chance Dr. Rufus L. Chaney Dr. John Peter Christopher Mrs. Diane P. Chun Mr. Dennis N. Cipollo Mrs. Ingrid Clever Dr. Stephen P. Coburn Dr. Richard H. Collier Dr. James M. Coull Miss Barbara M. Daly Dr. Dale George Deutsch Dr. Landis W. Doner Mr. Alvin L. Donoho Dr. William L. Ellefson Dr. Rhoda I. Feldman Ms. Valerie S. Ferguson Dr. Heinz G. Floss Dr. James D. Forney Dr. Renny Theodore Franceschi Mr. Phillip K. Freese Mr. Mark J. Fretz Dr. Jon A. Friesen Mr. Martin Gibbs Dr. David M. Gibson Dr. John F. Gill Jr. Dr. Melvin E. Gleiter Dr. Barbara L. Golden Mr. Robert E. Gramera Mr. Jeremy P. Green

Dr. Joseph L. Haining Dr. D. Michael Hampsey Mr. Raymond C. Hapak Dr. Nancy Harding Dr. Robert A. Harris Dr. Marilyn A. Hartig Dr. Harold J. Harwood Jr. Dr. John P. Hauschild Dr. Mark A. Hermodson Prof. Peter A. Hippensteel Mr. Harry H. Hirschl Mr. John W. Hollis Jr. Mr. Anthony H.C. Huang Dr. Paul W. Huber Dr. Paul P. Hung Dr. Douglas I. Johnson Dr. Dwight O. Kamback Jr. Dr. Gregory Kelly Dr. Claudia M. Kent Dr. Kenneth W. Kirby Dr. Gunter B. Kohlhaw Dr. William Kopaciewicz Dr. David W. Krogmann Dr. Chi W. Lai Dr. George E. Lauterbach Dr. Thomas G. Lawson Dr. Alice C. Layton Dr. Charles L. Leeck Dr. Marguerite Lambert Leng Dr. Albert Light Mr. Robert O. Lineberger Dr. Philip S. Low Mr. Merton W. Luce Dr. Sharon M. Machlik Dr. John E. MacNintch Dr. Jean Marx Dr. Michael P. Massagli Dr. Hiromu Matsumoto Mr. Steven D. Mavity Mr. Michael L. McAfee Dr. Clarence E. McDonald Dr. Donald J. McNamara Dr. Jonathan James Mitschelen Dr. Evelyn M. Mobley Dr. Owen Arnold Moe Jr. Mrs. Shirley P. Molinari Dr. Philip J. Monroe Dr. Henry A. Moses Mrs. Elizabeth C. Mundell Dr. John J. Murphy Mr. Alan L. Neal Mrs. Michelle Lee Neff Mrs. Cynthia M. Neville

Dr. Rodney J. Noel Mrs. Dawn M. Ogas Dr. Bryan Lawrence Ohning Mr. Robert E. Parson Jr. Mrs. Lynne M. Pattison Mr. Scott L. Pattison Dr. Edo D. Pellizzari Dr. Elfriede K. Pistorius Mr. Malcolm J. Pitts Dr. Thomas J. Porter Dr. David A. Pot Dr. Merton H. Pubols Dr. Lesley J. Putman Mr. Milan Radovich Mark J. Reasor, Ph.D. Dr. John Joseph Reiners Jr. Dr. Marvin S. Reitz Mrs. Kimberly C. Rhykerd Dr. James W. Ridlington Dr. Victor W. Rodwell Mr. Jerry D. Ropp Dr. Sandra Rossie Dr. Michael G. Rossmann Dr. Charles D. Rowe Dr. Robert B. Rucker Dr. Leslie A. Rylander Mr. Oliver C. Sabie Mr. Arthur G. Samaras Mrs. Samantha D. Samaras Mrs. Jill F. Sampson Mr. Randall Craig Schatzman Mr. David Stuart Scher Dr. Richard W. Schneeman Dr. Stephanie C. Schroeder Mr. Daniel Robert Schwartz Dr. Bradley T. Sheares Dr. Damon C. Shelton Mrs. V. M. Shelton Dr. Ihor Skrypka Dr. Richard G. Sleight Jr. Dr. C. Louis Smart Dr. Ronald L. Somerville Dr. John H. Stark Dr. Arnold Stein Dr. Rodger W. Stringham Dr. Barbara R. Sturm Dr. Autumn L. Sutherlin Dr. Marie A. Tavianini Dr. Irwin Tessman Dr. Edwin E. Traverso Dr. J. Yun Tso Dr. Edgar H. Ulm Dr. Eldon L. Ulrich

(

Donors Thank you to all who donated to the department. General gift donations help with the costs of recruiting faculty and stu-dents, lecture series, and support of our Undergraduate Biochemistry Club. Donations significantly increased the Axelrod Lectureship endowment this year, which allows us to continue to achieve the goal of bringing internationally recognized scholars to campus for seminars and informal interactions with our faculty and students. These gifts continue to be the sole source of funds for many activities that are critical to the development of the intellectual community within the de-partment.



Dr. Joseph J. Villafranca Dr. Lindley H. Wagner Dr. Robert G. Walker Dr. Daniel E. Walker Mr. Eugene O. Walker Mr. John A. Wallace Dr. Robert O. Webster Dr. Henry Weiner Dr. Leo V. Weitzman Mr. Jerome A. Welte Mrs. Anne Marie Wentworth Dr. Kathleen B Whitaker Mrs. Diane A. Wiginton Mr. Jeffrey B. Williams Dr. Joseph Wolinsky Mr. Michael J. Worns

Dr. Hao Wu Mrs. Kathleen M. Yang Dr. Howard Zalkin

Corporate Donors

Abbott Laboratories Fund Aventis Behring LLC Biogen Idec Bristol-Myers Squibb Fdn Inc Eli Lilly and Co. Fdn. Henkel Corporation Hormel Foods Corporation J.J.B. Hilliard, W.L. Lyons, Inc Merck Company Foundation Millipore Foundation

We apologize if any donor was omitted. Corrections will be placed in next year’s

annual report.

Donors (cont’d)


Publications

2004

Fingerman I.M. and S.D. Briggs. P53-mediated transcrip-tional activation: from test tube to cell. Cell 117: 690-691.

Liu X., M. Kremer and S. Broyles. A natural vaccinia virus promoter with exceptional capacity to direct protein syn-thesis. J. Virol. Methods 122: 141-145.

Humphreys J.M. and C. Chapple. Immunodetection and quantification of cytochromes P450 using epitope tag-ging: immunological, spectroscopic and kinetic analysis of cinnamate 4-hydroxylase. J. Immun. Methods 292: 97-107.

Rider S.D., M.R. Hemm, H.A. Hostetler, H-C. Li, C. Chapple and J. Ogas. Metabolic profiling of the Arabidopsis pickle mutant reveals selective derepression of embryonic traits. Planta 219: 489-499.

Wang W.Q., J. Bembenek, K.R. Gee, H. Yu, H. Charbon-neau and Z.Y. Zhang. Kinetic and mechanistic studies of a cell cycle protein phosphatase Cdc14. J. Biol. Chem. 279: 30459-30468.

Matsuda A., K. Mayer and J.D. Forney. Identification of single nucleotide mutations that prevent developmentally programmed DNA elimination in Paramecium tetraurelia. J. Eukaryot. Microbiol. 51: 664-669.

Hall M.C., E.N. Warren and C.H. Borchers. Multi-kinase phosphorylation of the APC/C activator Cdh1 revealed by mass spectrometry. Cell Cycle 3: 1278-1284.

Falcone D.L., J. Ogas and C.R. Somerville. Regulation of membrane fatty acid composition by temperature in mu-tants of Arabidopsis with alterations in membrane lipid composition. BMC Plant Biol. 4: 17.

Friesen J.A. and V.W. Rodwell. The 3-hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) reductases. Ge-nome Biol. 5: 248-252.

Hedl M. and V.W. Rodwell. Inhibition of the Class II HMG-CoA reductase of Pseudomonas mevalonii. Protein Sci-ence 13: 1693-1697.

Sutherlin A. and V.W. Rodwell. Multienzyme mevalonate pathway bioreactor. Biotechnol. Bioeng. 87: 546-551.

Manaves V., Q. Wuxuan, S.L. Bauer, S. Rossie, M. Koba-yashi and S. Rane. Calcium and vitamin D increase mRNA levels for the growth control hIKI channel in human epi-dermal keratinocytes but functional channels are not observed. BMC Dermatol. 4: 7.

Tian Q., M. Freethman, W.A. Tao, X. He, L. Li, R. Aebersold and L. Hood. Proteomic analysis identifies that 14-3-3zeta interacts with beta-catenin and facilitates its activa-tion by Akt. Proc. Natl. Acad. Sci. USA 101: 15370-15375.

Mukhopadhyah A., L. Ni and H. Weiner. A co-translational model to explain the in vivo import of proteins into HeLa cell mitochondria. Biochem. J. 382: 385-392.

Book Chapters

Rodwell V.W., M. Hedl, A.L. Sutherlin, D.A. Bochar, C.V. Stauffacher and L. Tabernero. Hydroxymethylglutaryl-CoA reductase, in xPharm, S.J. Enna and D.B. Bylund eds. Elsevier, New York.

Tao W.A. and R. Aebersold. Quantitative proteomics via mass spectrometry and stable isotope dilution, in Ency-clopedia of Mass Spectrometry on Biological Applica-tions, R. Caprioli and M. Gross eds. Wiley & Sons, New York.

Weiner H. and T.D. Hurley. Binding of NAD(P)+ to dehydro-genases, in Nature Encyclopedia of Life Sciences, John Wiley and Sons Ltd., Chichester.

2005

Fraser C.M., L.W. Rider and C. Chapple. An expression and bioinformatics analysis of the Arabidopsis serine car-boxypeptidase-like gene family. Plant Physiol. 138: 1136-1148.

Golden B., H. Kim and E. Chase. Crystal structure of a phage Twort group I ribozyme-product complex. Nat. Struct. and Mol. Biol. 12: 82-89.

Chase E. and B.L. Golden. Crystallization and preliminary diffraction analysis of a group I ribozyme from bacterio-phage Twort. Acta Cryst. F. F61: 71-74.

Doun S.S., J.W. Burgner II, S.D. Briggs and V.W. Rodwell. Enterococcus faecalis phosphomevalonate kinase. Pro-tein Sci. 14: 1134-1139.

Yan F., M. He, J.M. Hogan, S.S. Rossie and S.A. McLuckey. Targeted biomarker detection via whole pro-tein ion trap tandem mass spectrometry: thymosin beta(4) in a human lung cancer cell line. J. Mass. Spec-trom. 40: 444-451.

Liu F., K. Iqbal, I. Grundke-Iqbal, S. Rossie and C. X. Gong. Dephosphorylation of tau by protein phosphatase 5: im-pairment in Alzheimer's disease. J. Biol. Chem. 280: 1790-1796.



Osyama T., N. Isse, N. Kagawa, T. Kinaga, Y.D. Kim, M. Morita, K. Sugio, H. Weiner, K. Yasumoto and T. Kawa-moto. Tissue-distribution of aldehyde dehydrogenase 2 and effects of the ALDH2 gene-disruption and the expres-sion of enzymes involved in alcohol metabolism. Front. in Biosci. 10: 951-960.

Zullo S.J., W.T. Parks, M. Chloupkova, B. Wei, H. Weiner, W.A. Fenton, J.M. Eisenstadt and C.R. Merril. Stable trans-formation of CHO cells and human NARP cybrids confers oligomycin resistance (oli(r)) following transfer of mito-chondrial DNA-encoded oli(r) ATPase6 gene to the nu-clear genome: a model system for mtDNA gene therapy. Rejuvenation Res. Vol. 8: 19-28.

Ho K.K. and H. Weiner. Isolation and characterization of an aldehyde dehydrogenase encoded by the aldB gene of E. coli. J. Bacteriol. 187: 1067-1073.

Ho K.K., A. Allali-Hassani, T. Hurley and H. Weiner. Differ-ential effects of Mg2+ ions on the individual kinetic steps of human cytosolic and mitochondrial aldehyde dehydro-genases. Biochemistry 44: 8022-8029.

Luo M., A. Hopf, M. Tanurdzic, W. Wang, J.K. Weng, K. Arumuganathan, J. Carlson, C. Chapple, C. dePamphilis, D. Mandoli, J. Tomkins, R. Wing and J.A. Banks. Construc-tion of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: A resource for vas-cular plant comparative genomics. BMC Plant Biol. 5: 10.

Weng J.K., M. Tanurdzic, C. Chapple. Functional analysis and comparative genomics of expressed sequence tags from the lycophyte Selaginella moellendorffii. BMC Ge-nomics 6: 85.

Book Chapters

Stout J. and C. Chapple. The phenylpropanoid pathway in Arabidopsis: lessons learned from mutants in sinapate ester biosynthesis, in Recent Advances in Phytochemis-try, Volume 38, J.T. Romeo ed. Elsevier Press, Amster-dam, The Netherlands.

Ho K.K. and H. Weiner. Screening for variants of human liver cytosolic aldehyde dehydrogenase with altered prop-erties, in Enzymology and Molecular Biology of Carbonyl Metabolism 12, H. Weiner, R. Lindahl, B. Plapp, E. Maser eds. Purdue University Press, West Lafayette Indiana.

Jeng J.J., D. Goldman, C.J.P. Eriksson, A.T. Kallarahal, T.T.Y. Wang, H. Weiner and B.J. Song. Accumulation of hepatic acetaldehyde and reduced alcohol drinking in transgenic mice carrying the oriental variant of the hu-man aldehyde dehydrogenase 2 gene, in Enzymology and Molecular Biology of Carbonyl Metabolism 12, H. Weiner, R. Lindahl, B. Plapp, E. Maser eds. Purdue University Press, West Lafayette Indiana.

Publications (cont’d)

Executive Editor: Clint Chapple

Editor and Production Managers: Betty Barlow and Kristi Trimble

Chief Photographers: Anna Wilson and Paula Flores

This publication is published annually by the Purdue University Department of Biochemistry.

It is the policy of the Purdue University School of Agriculture that all persons shall have equal opportunity and access to the programs and facilities without regard to race, color, sex, religion, national origin, age, marital status, parental status, sexual orientation or disability.

Department of Biochemistry

Purdue University 175 S. University Street

West Lafayette, IN 47907-2063

http://www.biochem.purdue.edu

Phone: 765-494-1600

DEPARTMENT OF BIOCHEMISTRY from the Department of Biochemistry ... Seminar Speakers ... undertake a...

Documents

Transcript of DEPARTMENT OF BIOCHEMISTRY from the Department of Biochemistry ... Seminar Speakers ... undertake a...