Davis Mine Project By: Jason Jean and Janice Wing.
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Transcript of Davis Mine Project By: Jason Jean and Janice Wing.
![Page 1: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/1.jpg)
Davis Mine Project
By: Jason Jean and Janice Wing
![Page 2: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/2.jpg)
Davis mine
![Page 3: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/3.jpg)
Drainage below tailings pile
![Page 4: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/4.jpg)
Tailings pile
![Page 5: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/5.jpg)
Drainage below tailings pile
![Page 6: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/6.jpg)
Drainage below another tailings pile
![Page 7: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/7.jpg)
Sample of pyrite at Davis Mine
![Page 8: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/8.jpg)
Stream confluence upstream from A4
![Page 9: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/9.jpg)
Stream confluence up close
![Page 10: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/10.jpg)
Presentation Outline
•Reasons for our research project
•Methods
•Findings
•Obstacles
•Research continuation
•Impact on us
![Page 11: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/11.jpg)
Reasons for our research project
•Personal
•Professional
•Enrichment media
![Page 12: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/12.jpg)
Methods
• Sterile, anaerobic technique
• pH adjustment
• Inoculation
• Incubation
• Observations and Microscopy
![Page 13: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/13.jpg)
Acquiring mine water from well
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Collection bottles for Davis Mine groundwater
![Page 15: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/15.jpg)
Measuring well water depth
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Time to think
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Sterilize bottles in the acid wash
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Preparing enrichment media under anoxic conditions.
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pH adjustment
![Page 20: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/20.jpg)
Sterilize Postgate and Pfennig media before inoculation in the autoclave. Davis Mine Well media was not sterilized in the autoclave before inoculation.
![Page 21: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/21.jpg)
Sediment from Well 1, 3, 4 were used
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Opening sediment jars and inoculating sediment into media bottles under anoxic conditions
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Incubation and observation
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Storage and incubation of transfer bottles
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Findings
• Sulfur-reducing bacteria grew in Postgate pH 7.0
• Sulfur-reducing bacteria grew in Davis Mine Well 1 Groundwater media at pH 7.0
• Thread-like filaments were visible in sample jars that developed a black precipitate, but in no others.
• Desulfobulbus was successfully cultivated in Postgate media but was not successful in Pfennig.
• Orange film developed in Pfennig enrichment bottles.
![Page 26: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/26.jpg)
Enrichment bottles with Postgate’s medium at
pH 3.0 4.0 5.0 7.0
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Enrichment bottles with Pfennig’s medium at
pH 3.0 4.0 5.0 7.0
![Page 28: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/28.jpg)
Davis Mine Well 1 media at
pH 3.0 4.0 5.0 7.0
![Page 29: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/29.jpg)
Davis Mine Well 3 media at
pH 3.0 4.0 5.0 7.0
![Page 30: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/30.jpg)
Davis Mine Well 4 media at
pH 3.0 4.0 5.0 7.0
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Black precipitate formed in Postgate media of pH 7.0 inoculated with Desulfobulbus
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Black precipitate formed in transfers of Postgate media at pH 7.0
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Black precipitate formed in transfers of Davis Mine Well 1 media at pH 7.0
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Microscopy using a live/dead bio-stain with this fluorescent microscope. Pictures were taken.
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Microscopy of Postgate pH 3.0 sample inoculated with Well 1 sediment
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Microscopy of Postgate pH 4.0 inoculated with Well 4 sediment
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Postgate at pH 5.0, sediment from Well 4
Cluster
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Postgate pH 7.0 sediment from Well 4
Thread-like filament
![Page 39: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/39.jpg)
Postgate pH 7.0 sediment from Well 4
Dark spots of pyrite forming
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What is the orange film and sediment?
(Seen in all Pfennig media, here the sample is pH 3.0)
![Page 41: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/41.jpg)
Obstacles
• Time
• Equipment
• Anaerobic technique is rigorous
![Page 42: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/42.jpg)
Presence of oxygen
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Contamination by a fungus
![Page 44: Davis Mine Project By: Jason Jean and Janice Wing.](https://reader038.fdocuments.net/reader038/viewer/2022110321/56649ce15503460f949ab98a/html5/thumbnails/44.jpg)
Tough going
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Research continuation
• Dilution transfers and DNA extraction
• Continued incubation
• Postgate media with a variety of donors
• DM Well media with a variety of donors
• Mercy
• Iron-reducers
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Impact on us
• Better understanding of long-range research
• Lab and project research experience
• Integrate into classroom curriculum aligned with state frameworks
• Biology
• Environmental Science
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Questions ??
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The End