CY1115 VEGFR3, Phospho-Tyr1230...Phospho-VEGFR3 (Tyr1230/1231) Antibody Cat. No. CY1115...
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Phospho-VEGFR3 (Tyr1230/1231) Antibody Cat. No. CY1115 Applications: Detected MW: Species & Reactivity: Isotype: WB, IHC 198/130 kDa Human Rabbit IgG www.cellapplications.com 858-453-0848 800-645-0848 5820 Oberlin Dr. Suite 101 San Diego, CA 92121 BACKGROUND The Vascular Endothelial Growth Factor Receptor (VEGFR) family has three members: VEGFR-1 (also known as flt-1), VEGFR-2 (KDR/flk-1), and VEGFR-3 (FLT4). In VEGFR-3, the fifth Ig homology domain of the extracellular portion is proteolytically cleaved and the resulting polypeptides remain linked by two-disulfide bonds. 1 The ligands binding to VEGFRs belong to the VEGF family of growth factors, which has five cellular members: VEGF, placenta growth factor (PlGF), VEGF-B, VEGF-C, VEGF-D, and the recently cloned viral VEGF homologue VEGF-E. 2 VEGFR-3 is required for cardiovascular development during embryogenesis. In adults, this receptor is expressed in lymphatic endothelial cells. VEGF-C and VEGF-D are VEGFR3 ligands. The binding of VEGF ligands to VEGFRs activates VEGFR3 signaling, which regulates cardiovascular development, angiogenesis, and lymphangiogenesis. 3 Signaling by VEGFR3 involves the CrkII, PI-3 kinase, and Grb2 pathways, leading to activation of downstream JNK1/2, Akt, and Erk1/2. 4 Activation of VEGFR3 leads to phosphorylation of a number of tyrosine residues in the receptor cytoplasmic domain. Phosphorylation of Tyr1063 and 1068 on the activation loop is closely related with opening of receptor ATP-binding site. Other five tyrosines (Tyr1230, 1231, 1265, 1337 and 1363) in the C-terminal tail of VEGFR3 were shown to be phosphorylated upon ligand stimulation. However, only Tyr1230, 1231, and 1265 were phosphorylated in both VEGFR3-homodimers and VEGFR3-VEGFR2 heterodimers. Tyr1337 and Tyr1363 only can be phosphorylated in VEGFR3 homerdimers. 4 Particularly Tyr1230 and 1231 were shown to be involved in lymphendothelial cell DNA synthesis, migration, and apoptosis. 5 References: 1. Otrock, Z.K. et al. : Blood Cells Mol Dis. 38:258, 2007. 2. Taipale, J. et al.: Curr Top Microbiol Immunol. 237:85, 1999. 3. Su, J.L. et al.: Br J Cancer. 96:541, 2007. 4. Bahram, F. & Claesson-Wesh, L.: Pathophysiol. 17:253-61, 2010 5. Dixelius, J. et al: J. Biol. Chem. 278:40973-9, 2003 TECHNICAL INFORMATION Source: Phospho-VEGFR3 (Tyr1230/1231) Antibody is a rabbit polyclonal antibody raised against a short peptide from sequence surrounding and containing phosphorylated Tyr1230/1231 of human VEGFR3. Specificity and Sensitivity: This antibody detects phosphorylated VEGFR3 (Tyr1230/1231) proteins without cross-reactivity with other family members. Storage Buffer: PBS and 30% glycerol Storage: Store at -20°C for at least one year. Store at 4°C for frequent use. Avoid repeated freeze-thaw cycles. APPLICATIONS Application: *Dilution: WB 1:1000 IP n/d IHC 1:50 ICC n/d FACS n/d *Optimal dilutions must be determined by end user.
Transcript of CY1115 VEGFR3, Phospho-Tyr1230...Phospho-VEGFR3 (Tyr1230/1231) Antibody Cat. No. CY1115...
Phospho-VEGFR3 (Tyr1230/1231) Antibody Cat. No. CY1115
Applications: Detected MW: Species & Reactivity: Isotype:
WB, IHC 198/130 kDa Human Rabbit IgG
www.cellapplications.com 858-453-0848 800-645-0848 5820 Oberlin Dr. Suite 101 San Diego, CA 92121
BACKGROUND The Vascular Endothelial Growth Factor Receptor (VEGFR) family has three members: VEGFR-1 (also known as flt-1), VEGFR-2 (KDR/flk-1), and VEGFR-3 (FLT4). In VEGFR-3, the fifth Ig homology domain of the extracellular portion is proteolytically cleaved and the resulting polypeptides remain linked by two-disulfide bonds.1 The ligands binding to VEGFRs belong to the VEGF family of growth factors, which has five cellular members: VEGF, placenta growth factor (PlGF), VEGF-B, VEGF-C, VEGF-D, and the recently cloned viral VEGF homologue VEGF-E.2 VEGFR-3 is required for cardiovascular development during embryogenesis. In adults, this receptor is expressed in lymphatic endothelial cells. VEGF-C and VEGF-D are VEGFR3 ligands. The binding of VEGF ligands to VEGFRs activates VEGFR3 signaling, which regulates cardiovascular development, angiogenesis, and lymphangiogenesis.3 Signaling by VEGFR3 involves the CrkII, PI-3 kinase, and Grb2 pathways, leading to activation of downstream JNK1/2, Akt, and Erk1/2.4
Activation of VEGFR3 leads to phosphorylation of a number of tyrosine residues in the receptor cytoplasmic domain. Phosphorylation of Tyr1063 and 1068 on the activation loop is closely related with opening of receptor ATP-binding site. Other five tyrosines (Tyr1230, 1231, 1265, 1337 and 1363) in the C-terminal tail of VEGFR3 were shown to be phosphorylated upon ligand stimulation. However, only Tyr1230, 1231, and 1265 were phosphorylated in both VEGFR3-homodimers and VEGFR3-VEGFR2 heterodimers. Tyr1337 and Tyr1363 only can be phosphorylated in VEGFR3 homerdimers.4 Particularly Tyr1230 and 1231 were shown to be involved in lymphendothelial cell DNA synthesis, migration, and apoptosis.5
References: 1. Otrock, Z.K. et al. : Blood Cells Mol Dis. 38:258, 2007. 2. Taipale, J. et al.: Curr Top Microbiol Immunol. 237:85, 1999. 3. Su, J.L. et al.: Br J Cancer. 96:541, 2007. 4. Bahram, F. & Claesson-Wesh, L.: Pathophysiol. 17:253-61, 2010 5. Dixelius, J. et al: J. Biol. Chem. 278:40973-9, 2003 TECHNICAL INFORMATION Source: Phospho-VEGFR3 (Tyr1230/1231) Antibody is a rabbit polyclonal antibody raised against a short peptide from sequence surrounding and containing phosphorylated Tyr1230/1231 of human VEGFR3. Specificity and Sensitivity: This antibody detects phosphorylated VEGFR3 (Tyr1230/1231) proteins without cross-reactivity with other family members.
Storage Buffer: PBS and 30% glycerol Storage: Store at -20°C for at least one year. Store at 4°C for frequent use. Avoid repeated freeze-thaw cycles. APPLICATIONS
Application: *Dilution: WB 1:1000 IP n/d
IHC 1:50 ICC n/d
FACS n/d *Optimal dilutions must be determined by end user.
Phospho-VEGFR3 (Tyr1230/1231) Antibody Cat. No. CY1115
Applications: Detected MW: Species & Reactivity: Isotype:
WB, IHC 198/130 kDa Human Rabbit IgG
www.cellapplications.com 858-453-0848 800-645-0848 5820 Oberlin Dr. Suite 101 San Diego, CA 92121
QUALITY CONTROL DATA
Top: Western Blot detection of phosphorylated VEGFR3 (Tyr1230/1231) proteins in cell lysates from PAE/VEGFR3 cells stimulated or untreated with VEGF-C using phosphor-VEGFR3 (Tyr1230/1231) antibody (Upper), and VEGFR3 antibody (Cat# CB5792) (Lower) (The cell lysates were provided by Dr. Lena Claesson-Welsh’s Lab of Uppsala University, Sweden). Bottom: This antibody stains paraffin-embedded human lung cancer tissue in immunohistochemical analysis.
