CV 2015
2
Pooja Patel 3302 Birch Creek Dr. Kingwood, TX 77339 [email protected] (832)964-8744 OBJECTIVE Ambitious and versatile molecular graduate seeking to further excel in the molecular diagnostic field through laboratory technician experience. EDUCATION The University of Texas at Austin, Austin, TX Aug 2010—May 2014 Bachelor of Science in Biochemistry The University of Texas at MD Anderson, Houston, TX Aug 2014—Aug 2015 Bachelor of Science in Molecular Genetic Technology GPA: 3.91 CERTIFICATION Molecular Biology (ASCP) CM Aug 2015—Aug 2017 LABORATORY EXPERIENCE University of Texas at MD Anderson Molecular Genetic Technology Performed DNA extraction, DNA quantitation, bisulfite treatment, variations of polymerase chain reaction, and Sanger sequencing Applied molecular biology to troubleshooting different molecular laboratory techniques Optimized polymerase chain reactions using bioinformatics Molecular Health Intern Prepared a genomic library for Next Generation Sequencing Illumina ® HiSeq platform Analyzed a variant list generated from a former Illumina ® HiSeq run in order to generate a list of effective treatment options Briefed company members on emerging challenges and findings in solid tumor cancers and therapies University of Texas Medical Branch Intern Comprehended the testing methods behind testing for infectious disease Performed and evaluated HCV viral load using real—time PCR University of Texas at Austin, Zhang Lab Undergraduate Researcher Half-activated and purified wild-type and C20IV mutant of human asparagine-like protein I using nickel column and HPLC purification systems Performed prep analysis for Human Asparagine-like Protein I wild-type/mutant Updated current protein purification procedures to maximize protein yield Received the Undergraduate Research Fellowship for research on investigating Asparagine—like Protein I mutant structure to understand the wild—type protein’s function as a replacement therapy for current treatments used in Acute Lymphocytic Leukemia LABORATORY SKILLS PROFICIENT IN: DNA Extraction o Peripheral Blood and Buccal Sample Qiagen DNA Extraction Kit, Gentra Puregene Kit Quality Control—Spectrophotometry and Nanodrop
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Transcript of CV 2015
Pooja Patel
3302 Birch Creek Dr. Kingwood, TX 77339 [email protected]
(832)964-8744 OBJECTIVE
Ambitious and versatile molecular graduate seeking to further excel in the molecular diagnostic field through laboratory technician experience.
EDUCATION
The University of Texas at Austin, Austin, TX Aug 2010—May 2014 Bachelor of Science in Biochemistry The University of Texas at MD Anderson, Houston, TX Aug 2014—Aug 2015 Bachelor of Science in Molecular Genetic Technology
GPA: 3.91 CERTIFICATION
Molecular Biology (ASCP)CM Aug 2015—Aug 2017 LABORATORY EXPERIENCE
University of Texas at MD Anderson
Molecular Genetic Technology
Performed DNA extraction, DNA quantitation, bisulfite treatment, variations of polymerase chain reaction, and Sanger
sequencing
Applied molecular biology to troubleshooting different molecular laboratory techniques
Optimized polymerase chain reactions using bioinformatics
Molecular Health Intern
Prepared a genomic library for Next Generation Sequencing Illumina® HiSeq platform Analyzed a variant list generated from a former Illumina® HiSeq run in order to generate a list of effective treatment
options Briefed company members on emerging challenges and findings in solid tumor cancers and therapies
University of Texas Medical Branch Intern
Comprehended the testing methods behind testing for infectious disease Performed and evaluated HCV viral load using real—time PCR
University of Texas at Austin, Zhang Lab Undergraduate Researcher
Half-activated and purified wild-type and C20IV mutant of human asparagine-like protein I using nickel column and
HPLC purification systems
Performed prep analysis for Human Asparagine-like Protein I wild-type/mutant
Updated current protein purification procedures to maximize protein yield
Received the Undergraduate Research Fellowship for research on investigating Asparagine—like Protein I mutant
structure to understand the wild—type protein’s function as a replacement therapy for current treatments used in
Acute Lymphocytic Leukemia
LABORATORY SKILLS
PROFICIENT IN:
DNA Extraction
o Peripheral Blood and Buccal Sample
Qiagen DNA Extraction Kit, Gentra Puregene Kit
Quality Control—Spectrophotometry and Nanodrop
Polymerase Chain Reaction Conventional, multiple targets set up in different reactions, Multiplex PCR, Touchdown
PCR, “Hot—Start”, Reverse Transcription PCR
SDS—PAGE and Agarose Gel Preparation and Run
Sanger Sequencing (ABI 310 Genetic Analyzer)
Bioinformatics
o NCBI databases including GenBank, RefSeq, BLAST suite, OMIM, dbSNP, HaploMap, GENE
o Other databases such as UCSC, Ensembl, Protein Data Bank
PyMOL
High—Performance Liquid Chromatography
o Ion Exchange
o Size Exclusion
Protein Expression and Purification
Crystal Tray Set—Up
EXPERIENCE WITH:
Formalin—Fixed Paraffin—Embedded Tissue Section DNA Extraction
RNA Extraction
o Peripheral Blood and Buccal Samples
Organic extraction using TRIzol reagent
QC—Nanodrop and native agarose gel electrophoresis
Real—Time PCR, Methyl—Specific PCR
Protocol Development
o Primer Design
o Polymerase Chain Reaction Optimization
Restriction Fragment Length Polymorphism Analysis
Library preparation for Illumina® HiSeq
FAMILIAR WITH:
Microsatellite Instability Analysis
Profiling by Short Tandem Repeat Analysis
Hybrid Capture
Microarray
Fluorescence In Situ Hybridization
Pyrosequencing
Next Generation Sequencing
o Ion Torrent™ Personal Genome Machine
o Illumina® Hiseq 2500 Library Preparation
AWARDS/ACHIEVEMENTS/CERTIFICATIONS
The Undergraduate Research Fellowship at the Spring 2014
University of Texas at Austin
Biology Scholars Program at the University of
Spring 2012
Texas at Austin
LEADERSHIP EXPERIENCE
The Project Senior Team Leader
Coordinated with community counsel and The Project members to improve impoverished Austin communities Planned and executed the restoration of a convent in a neglected community Corresponded with community leaders in order to obtain the necessary materials and volunteers for the restoration
REFERENCES AVAILABLE UPON REQUEST
