CUTTING-EDGE INNOVATIVE BIOCHIPS

CUTTING-EDGEINNOVATIVE BIOCHIPS

Single-Cell Cloning & Sequencing

Rare Cell Isolation

Automated Lifespan Assay

Bacterial Endotoxins Test Platform

CONTENTS

Company 3

Single-Cell Cloning & Sequencing 4

Rare Cell Isolation 5

● 1CellPlate 6

● Smart Aliquotor 8

● Smart Aliquotor CE 10

● Smart Aliquotor mini 12

● Million Microwell Device 14

Automated Lifespan Assay 16

● Automated Dissection Chip 17

● A1 Cell Imaging System 19

Bacterial Endotoxins Test Platform 21

● LOFT Plate 22

● Microplate Reader 24

● Benchtop Centrifuge 26

Order Information 28

Global Distributors 30

COMPANY

Innovative Biochips (a.k.a. iBiochips) is a spin-off company from Houston Texas Medical Center, the largest medical complex in the world.

Innovative Biochips is committed to develop advanced microfluidics technologies that resolve traditional biotech challenges by achieving cutting-edge sensitivity and high-throughput. Innovative Biochips’ scientists and engineers have extensive and successful experience in developing novel microfluidics technologies for various biological and biomedical applications.

Innovative Biochips has established strategic partnerships with Thermo Fisher Scientific, Millipore Sigma, BioTek, Grant Instruments, and Harvard Bioscience that allow key benefits to drive product development.

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Single-Cell Cloning & Sequencing

Successful single-cell isolation is a primary step for subsequent single-cell analysis, mainly including 1) single-cell cloning from single adherent cell, single suspension cell, or single CRISPR-edited cell, and 2) single-cell PCR & sequencing which highly requires a convenient and reliable method to rapidly isolate a viable and intact cell into a specific container such as a PCR tube.

The 1CellPlate and Smart Aliquotor series of products overcomes current single-cell isolation technical challenges in time-consuming, operational complexity, limited efficiency, deterioration of cell viability and integrity, and incompetence in the isolation of single cells into small-volume liquids to perform single-cell PCR & sequencing. The 1CellPlate and Smart Aliquotor series of products provides unprecedented simplicity and convenience in the single-cell isolation with high efficiency and high tolerance of cell size, shape, and numbers.

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Rare Cell Isolation

There is accumulating evidence showing the existence of phenotypic and genotypic heterogeneity among various cell populations. Therefore, single-cell analysis, especially the efficient isolation of desired single rare cells from heterogeneous bulk populations for the downstream single-cell clonal & genetic analysis have become increasingly significant for investigations of clonal generation with/without genome editing, tumor evolution, embryonic development, and immunological intervention. The initial and crucial step to perform the above mentioned analysis is to selectively isolate intact single rare cells from heterogeneous background cells.

The 1CellPlate provides a quick and easy way to isolate single rare cells for subsequent single-cell clonal analysis and genetic analysis. One rare cell can be enriched ~3 × 104 times by using one 1CellPlate and ~1 × 109

times by using two 1CellPlate.

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Specifications Description

Material Polystyrene (medical grade)

Size of Device Standard 96-well plate form factor

Number of Wells 96 wells

Size of Well 2 mm (Diameter), 2 mm (Height)

# of Single Cells Can Be Isolated ~ 30 single cells per plate

Volume of Single Cell Retrieval 3 – 6 µL per well

Compatible Cell Size ≤ 80 µm (Diameter)

Bottom Coating of Well Tissue culture treated

Sterilization Sterile

1CellPlate®

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3

1

2

Bulk Cells AddingS

tep

s T

o G

et

Sin

gle

Ce

lls

~ 30 cells / 100-200 µL per inlet port

Single-Cell Isolation

3-6 µL single cell volume per well

Single-Cell Identification

~ 30 single cells per plate

Efficiently Retrieve Single Cells for PCR & Sequencing

Bright field Fluorescence Bright field Fluorescence

Easily Isolate Single Viable Cells to Generate Cloning

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Day 1 Day 2Day 3 Day 7 Day 10

Before retrieval After retrieval

Smart Aliquotor


Material Biocompatible polystyrene & PDMS

Size of Device Standard 100 mm petri dish form factor


Size of Well 1.8 mm (Diameter), 1 mm (Height)

# of Single Cells Can Be Isolated ~ 30 single cells per device

Volume of Single Cell Retrieval 2– 3 µL per well




Single cell

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Easy Way to Get Single Cells for Downstream Analysis

Single-Cell Isolation Single-Cell Cloning Rare-Cell Isolation

● Easy to operate by regular pipette

● High integrity for single-cell PCR

● High cell viability for clonal analysis

● Easy to track whole cloning process

● From CRISPR gene edited cells

● From clinical samples

Competitive Advantage of Smart Aliquotor

EasyOperation

LowCost

Cell Viability& Integrity

CellCloning

Single-cell PCR & Seq

Rare Cell Isolation

Compatible With Few Samples

Smart Aliquotor

Serial Dilution

Micro-manipulation

Flow Cytometry

Microwell &Droplet Systems

Performance: Intermediate PoorExcellent9



Size of Device Standard 60 mm petri dish form factor



# of Single Cells Can Be Isolated ~ 20 single cells per device





Single cell

Smart Aliquotor CE

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Single-Cell Cloning

Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7

Single cell

4X

20X

E7



Single-Cell Retrieval

Isolated single cell

Retrieved single cell

Rare-Cell Isolation

Flu

ore

sce

nce

4X

(O

bje

ctiv

e)

Me

rge

20X

(O

bje

ctiv

e)

After1st Isolation

After2nd Isolation

BeforeIsolation

1 target cell/GFP in ~1,000 non-GFP cells Retrieved target cell

1 target cell/GFP in 16 non-GFP cells

Only 1 target cell in the well of # F4

• Easy to operate by regular pipette

• High integrity for single-cell PCR

• From CRISPR gene edited cells

• From clinical samples

• High cell viability for clonal analysis

• Easy to track whole cloning process

Estimated Number of Isolated Single Cells per Smart Aliquotor CE

Retrieval of Target Cell

# o f s p ik e d c e lls#

of

iso

late

d s

ing

le c

ell

s

iso

lati

on

eff

icie

nc

y

0 2 0 4 0 6 00

1 0

2 0

3 0

0 .4

0 .6

0 .8

1 .0

Experiment

Simulation

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Size of Device Standard 6-well plate form factor



# of Single Cells Can Be Isolated ~ 60 single cells per plate





Smart Aliquotor mini

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Singlecell


Single-Cell Cloning

• Easy aliquoting by regular pipette

• Enable to isolate single cells from 6 different cells

• High cloning efficiency due to high cell viability

• Easy to track whole cloning process in 1.8mm well

Single-Cell Retrieval• Efficient retrieval by regular pipette

• Transfer an isolated single cell in 1.5 µL volume

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Before Retrieval After Retrieval

10X

Ob

ject

ive

Day 1 Day 2 Day 3

Day 6 Day 8 Day 10

10X

10X

Before Aliquoting After 6 Times of Aliquoting

Million Microwell Device

MMD

Capture single cells

Wash away uncaptured beads

Wash away uncaptured cells

Single-cell lysis

Capture barcoded magnetic beads

1,000,000 High-quality Microwells Workflow for scRNA-Seq

● Bead retrieval

● RT, PCR & library

● Sequencing & analysis

Cross-section

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Capture of GFP-labeled cells and magnetic beads in MMD

Bright Field GFP Merge

Microwellswith cellsand beads

One microwell with one cell and one bead

Magnetic bead

GFP labeled cell

Microwellswith cells


Material of Device PDMS

Size of Device 62 mm x 62 mm

Thickness of Device 2 mm

Diameter of Well 30 µm (Diameter)

Depth of Well 33 µm

Number of Well 1,000,000

● The Magnet Kit for MMD is available.

● We provide custom-made microwell- & micropillar-array devices with desired dimensions.

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Automated Lifespan Assay

Advancing our understanding of the underlying molecular mechanisms of aging, as well as their contributions to age-associated diseases, will have a profound impact on public health. Studying the replicative aging phenomenon in the budding yeast Saccharomyces cerevisiae has led to significant findings on how aging is regulated by evolutionarily conserved enzymes and molecular pathways. However, the laborious and low-throughput methods of current yeast replicative lifespan assays limit their usefulness as a broad genetic screening platform for research on aging.

Automated Dissection Chip (AD Chip) provides automated whole-lifespan tracking of over 10,000 single yeast cells for 20 different strains in 3 days with time-lapse microscopy. The AD Chip enables high-resolution fluorescent imaging of single-cells during the entire aging process, allowing for investigation of gradual changes in molecular markers on the single-cell scale and high-throughput examination of the aging phenotype, including organelle morphology, gene expression, and protein localization to identify genetic or environmental factors that regulate lifespan.

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Automated Dissection Chip

Applications

High-Throughput

Replicative Lifespan Assay

Genetic Screening for Longevity Associated Genes

Proteomic Screening for Protein Turnover and Relocalization

Nutrient Sensing and

Signaling Pathways

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AD Chip with U-shaped Traps (AD Chip-U)

AD Chip with Y-shaped Traps (AD Chip-Y)

Features of Automated Dissection Chip

Specifications of Automated Dissection Chip

Material • Biocompatible polymer

Dimensions • 75mm (Length), 25mm (Width), 4mm (Height)

Substrate • Glass coverslip (0.16 mm thickness) allowing up to 100X oil objective

Number of Traps • Total 18,000 traps (900 traps x 20 separate channels)

Number of Inlets • 20 media & cells inlets

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High-Throughput to Reduce Labor & TimeContinuous and automatic dissection of daughter cells without disturbing mother cells, allowing high-throughput lifespan assay of over 10,000 single yeast cells for 20 different strains in 3 days

High Resolution Fluorescent ImagingCompatible with high resolution fluorescent imaging for gene expression and molecular markers assays on single-cell level during entire aging process

Maintaining Constant Growth Condition Supply of continuous flow of fresh media, minimizing variations introduced by operators and environment

Tracking of Virgin Cells (with AD Chip-Y)Enable to assay starting from virgin cells, never having previously produced a daughter cell.

AD Chip-U AD Chip-Y

Reference: M.C. Jo, et al. “High-throughput analysis of yeast replicative aging using a microfluidic system.” PNAS, vol. 112 (2015), 9364‒9369.

A1 Cell Imaging System

The A1 Cell Imaging System is a fully automated, digital, inverted multi-channel fluorescence and transmitted light imaging system. The system is designed for a broad range of applications including, but not limited to, multi-channel fluorescence imaging, multiple-position vessel scanning, area scanning with montage or tile stitching, and time-lapse imaging.

Features

● New high-resolution CMOS cameras for improved resolution, sensitivity, and higher image quality (dual color and monochrome cameras)

● Enhanced scan speed and autofocus functions for improved throughput and data quality

● Fully automated and motorized X/Y scanning stage, refined autofocus, and multiple options for automation routines

● Simultaneous acquisition in 4 fluorescence channels and transmitted light

● Powerful PC with graphics processing unit (GPU) for fast processing of large data sets and demanding visualization applications

● Compatibility with the Onstage Incubator for precise control of temperature, humidity, and gases for normoxic or hypoxic conditions allows a wide range of biological studies under physiological conditions

Microfluidic Devices

iBiochips provides development and manufacturing of customized microfluidic devices based on your desired dimensions and functions.

Applications

● Neurobiology

● Immuno-oncology

● Live-cell imaging

● 3D cell imaging

● High-resolution tile scanning

● Immunohistochemistry (IHC)

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A1 Cell Imaging System Specifications

Optics Infinity‐corrected optical system; Royal Microscopical Society (RMS) threaded objectives with a 45 mm parfocal distance

Illumination LED light cubes (>50,000-hour life per light cube) with adjustable intensity

Fluorescence Channels Simultaneously accommodates 4 fluorescence cubes plus bright-field imaging

Contrast Methods Fluorescence and transmitted light (bright-field and phase-contrast)

Objective Capacity 5-position turret; front-mounted control

Objectives Wide selection of high-quality, long working distance (LWD), and coverslip-corrected objectives ranging from 1.25x to 100x

Condenser 60 mm LWD condenser, 4-position turret with a clear aperture and 3-phase annuli

Stage Motorized X/Y scanning stage; 120 x 80 mm travel range with submicron resolution; drop-in inserts to receive vessel holders and lockdown holders to fix sample during long scans

LCD Display 23” high-resolution touch-screen color monitor; 1,920 x 1,080 pixel resolution

Cameras High-sensitivity 3.2 MP (2,048 x 1,536) monochrome & color CMOS sensors with 3.45 μm pixel resolution

Captured Images 16-bit RAW monochrome: TIFF, PNG; 8-bit TIFF, PNG, JPG; Movies and time-lapse images: AVI, WMV

Dimensions (L x W x H) 46 x 33 x 36 cm (18 x 14 x 13 in.)

Onstage Incubator When combined with the Onstage Incubator, the A1 Cell Imaging System is ideal for long-term monitoring of cell cultures and time-lapse imaging at high resolution. The Onstage Incubator is an environmental chamber that allows for precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and nonphysiological conditions, making the system ideal for demanding hypoxia experiments.

Onstage Incubator Specifications

Compatible Vessels Multiwell plates, 35, 60, and 100 mm petri dishes, T-25 flasks, chamber slides, and more

Temperature Range Ambient to 40°C

CO2 Range 0 – 20%

O2 Range 0% to ambient

Humidity Range > 80% relative humidity at 37°C

Dimensions (L x W x H) 25 x 19 x 3.7 cm (environmental chamber), 37 x 16 x 20 cm (control unit)

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Bacterial Endotoxins Test Platform

Endotoxin, originating from the cell membrane of gram-negative bacteria, causes strong immune response resulting symptoms such as fever, nausea and septic shock. It is required by a regulation to test the level of endotoxin in medical products, including implants, human and animal parenteral drugs and vaccine, medical equipment, and all products labeled as non-pyrogenic.

iBiochips endotoxin testing platform offers more precise kinetic endotoxin assay with reduced reagent consumption. Innovative microfluidic flow control integrated in standard well plate eliminate source of experimental and environmental errors, while it is compatible with existing endotoxin test system.

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LOFT Plate®

Save 85% reagents

Eliminate evaporation

Prevent contamination

Improve accuracy

Meet USP. EP. and JP. standards

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Easy Operation Steps

● Save 85% LAL and endotoxin reagent consumption

● Skim air bubbles

● Improve readout accuracy by a fixed optical path

● Assist mixing by centrifugation

● Eliminate evaporation and environmental contamination by dual optical windows

● Compatible with existing endotoxin test system

Innovative LOFT Plate®

Add Sample Centrifuge Readout

It is an endotoxins test plate at another level.

Sample level

Detection level

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Microplate Reader

High quality optics and rugged design are the hallmarks of the ELx808™ Absorbance Microplate Reader. The 340 nm – 900 nm wavelength range encompasses a wide range of potential applications for this multichannel reader.

The ELx808 offers unsurpassed superior 4-Zone™ incubator, providing excellent stability for temperature sensitive assays like endotoxin analysis and long-term bacteria and yeast growth studies.

The ELx808 is controlled by Gen5 Reader Control Software for rapid data collection, export and printing. Other available Gen5 editions offer powerful data analysis for end point and kinetic measurements, along with customizable reporting and exporting features.

Applications

● Endotoxin Assays

● Protein Assays

● ELISA

● Enzyme Kinetics

● Cell Growth Studies

● Cytotoxicity

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Specifications

Detection Method Absorbance

Read Method End-point, kinetic, linear scanning

Microplate Types 96 wells

Temperature Control Incubation to 50 °C

Shaking Linear

Software Gen5 Reader Control Software included

Light Source Tungsten halogen

Wavelength Selection Filters

Wavelength Range 340 – 900 nm

Dynamic Range 0 – 4.0 OD

Resolution 0.001 OD

Filter Wheel Capacity 6 positions

OD Accuracy < 1% at 2.5 OD, < 2% at 3.5 OD

OD Linearity < 1% at 2.5 OD

OD Repeatability < 0.5% at 2.5 OD, < 1.5% at 3.5 OD

Reading Speed 8 seconds for 96 wells

● Incubation provides improved reproducibility for temperature-sensitive assays

● Fast kinetic measurements in intervals as short as 6 seconds with multiple kinetic analysis options

● Superior optical design incorporates staggered optics to eliminate channel-to-channel cross-talk

● End point, kinetic and linear well scanning read modes

● Gen5 Reader Control Software included

Features

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Benchtop Centrifuge

The LMC-3000 is a low speed benchtop centrifuge with interchangeable rotors for accommodating microplates and centrifuge tubes (2 to 50 mL). It is suitable for a wide range of analytical applications including biomedical, bio-organic and immunoenzyme analysis.

Features

● Spin speed: up to 3000 rpm for tubes, up to 2000 rpm for microplates

● Timed operation (1 to 90 minutes), with automatic switch-off

● Convenient interchangeable rotor for 6 or 12 tubes or 2 microplates provides flexibility for an easy change in application

● Easy set-up of spin speed and time via 2-line LCD display and simple push buttons

● Sturdy metal housing and lid, automatic imbalance switch-off and lid lock when centrifuge is running provide for safe operation in your workplace

● Compact design with small footprint

● The powerful and extremely quiet motor has a ‘soft’ start-up and run-down function to prevent possible damage to samples from sudden jolting.

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Specifications

Max RCF (Bottom of tube) 1700 x g

Speed (Centrifuge tubes) 100 – 3000 rpm

Max RCF Microplate Rotor 560 x g

Speed (Microplates) 100 – 2000 rpm

Centrifugation Time Up to 90 minutes ( 1 min increment)

Speed Increment 100 rpm

Temperature Range 4 – 40 °C (suitable for use in cold rooms)

Chamber Diameter 335 mm

Overall Dimensions 420 mm (W), 495 mm (D), 235 mm (H)

Accessories

R-2 centrifuge rotor for 2 microplates

• Dimensions: 128mm (W) x 85.6mm (D) x 45mm (H)

• Suitable for deep well plates

R-6 centrifuge rotor for 6 x 50mL tubes

• With cap, Conical end

• Dimensions: ø 29 mm x 115 mm

R-12-10 centrifuge rotor for 12 x 10 to 15mL tubes

• No cap, Rounded end


R-12-15 centrifuge rotor for 12 x 15mL tubes

• With cap, Conical end


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Order Information

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You can order Innovative Biochips’ products through Fisher Scientific. Search by Fisher catalog numbers.

Product Cat. No. Fisher Cat. No.

1CellPlate, 10pack P1-SCP-10PK NC1880660

Smart Aliquotor, 1pack H1-SA-1PK NC1637875

Smart Aliquotor, 5pack H1-SA-5PK NC1637877

Smart Aliquotor Compact Edition (CE), 5pack H2-SACE-5PK NC1637881

Smart Aliquotor mini, 5pack H3-SAMINI-5PK NC1941871

Million Microwell Device, 5pack H4-MMD-5PK NC1637882

Automated Dissection Chip, 4pack A1-AD-4PK NC1637884

LOFT Plate, 10pack P2-LP-10PK NC1880661

Order Information

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Product Cat. No.

A1 Cell Imaging System A1-IBE-7000

Microplate Reader A3-ELX-808

Benchtop Centrifuge A3-LMC-3000

Dual Syringe Infusion Pump A1-IBL-200

Multi-Syringe Infusion Pump A1-IBL-300

Microfluidics Tubing MTB-100

Microfluidics Syringe Tip LSA-100

Microfluidics Hollow Pin HSP-50

Magnet Kit for Million Microwell Device H4-MAG-1P

Global Distributors

Belgium

Tebu-biohttps://www.tebu-bio.comPhone: 03 454 00 66Email: [email protected]

China

Nuowei Biotechnologyhttp://www.nwbiotec.comPhone: +86 010-57438396Email: [email protected]

France

Tebu-biohttps://www.tebu-bio.comPhone: 01 30 46 39 00Email: [email protected]

France

Darwin Microfluidicshttp://www.darwin-microfluidics.comPhone: +33 184 163 [email protected]

Germany

Tebu-biohttps://www.tebu-bio.comPhone: 069 801013-0Email: [email protected]

Israel

Doron Scientifichttps://www.doronscientific.comPhone: +972-(0)54-5544004Email: [email protected]

Italy

Tebu-biohttps://www.tebu-bio.comPhone: 02 97 29 50 10Email: [email protected]

Japan

NT Sciencehttps://www.nt-science.comPhone: +81-568-97-5142Email: [email protected]

Netherlands

Tebu-biohttps://www.tebu-bio.comPhone: 072-572 21 00Email: [email protected]

Nordic Countries

Tebu-biohttps://www.tebu-bio.comPhone: +45 46 74 02 54Email: [email protected]

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Portugal

Tebu-biohttps://www.tebu-bio.comPhone: +351 215 842 958Email: [email protected]

Singapore, Malaysia, Thailand

Genomax Technologieshttps://www.genomax.com.sgPhone: 65-6777 1886Email: [email protected]

Spain

Tebu-biohttps://www.tebu-bio.comPhone: 93 306 34 10Email: [email protected]

South Korea

Woongbee Meditechhttp://www.woongbee.comPhone: +81-2-881-5432Email: [email protected]

South Korea

Edithgenhttp://www.edithgen.comPhone: +81-031-366-8952E-mail: [email protected]

Taiwan

Omics Biotechnologyhttp://www.omicsbio.com.twPhone: +886-2-8698-2268Email: [email protected]

Taiwan

BIOTOOLShttps://www.toolsbiotech.comPhone: +886-2-2697-2697Email: [email protected]

Taiwan

PAN ASIA Biomedical Technologyhttp://www.pan-asia-bio.com.twPhone: +886-2-2741-8169Email: [email protected]

UK & Ireland

Tebu-biohttps://www.tebu-bio.comPhone: +44 (0)1733 421880Email: [email protected]

United States

Fisher Scientifichttps://www.fishersci.comPhone: [email protected]

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