Curriculum Vitae THI HUONG NGUYEN, PhD Contact · CV_____Dr. Thi Huong Nguyen 1 Curriculum Vitae...
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CV_____Dr. Thi Huong Nguyen 1 Curriculum Vitae THI HUONG NGUYEN, PhD Contact Address Rosenhof 1, 37308 Heilbad Heiligenstadt, Germany Tel +49 (0)3606 671 600 Fax +49 (0)3606 671 200 Email [email protected] Thi-Huong Nguyen studied bachelor and master’s degree in chemistry at the Hanoi University of Science, Vietnam. She worked at the Institute of Chemistry, Vietnamese Academy of Science and Technology, Vietnam on synthesizing the (17-a)- Ethynylestradiol (7/2000 - 2/2004). She moved to South Korea for her PhD study in Physics and Nanoscience, Department of Physics and Nanoscience, Sun Moon University South Korea(3/2004-8/2008). Her thesis focused on adhesion forces between l-DNA and different modified substrates. She then worked as a research assistant at the Nano/bio Research Center, Korea Institute of Science and Technology (KIST), Seoul, South Korea (10/2008-9/2009) on the projects enhancing accuracy of the elasticity measurement of a single double-strand DNA by atomic force microscopy and identifying the correlation of astrocyte stiffness with cytoskeletal maturation. She then studied her PhD in chemistry at the Max-Planck Institute for Polymer Research and Institute of Pharmacy and Biochemistry, the University of Mainz, Germany (10/2009-4/2013). Her thesis title is ‘Rupture Forces of Split Aptamers’. She joined the Nanostructure group at the Center for Innovation Competence for Humoral Immune Responses in Cardiovascular Disease (ZIK HIKE), University of Greifswald, Germany for 1.5 year postdoc. Her work focused on gaining insights into the interactions between platelet factor 4 and heparins of different lengths and interaction among platelets with different degrees of activation. Then, she moved to the Institute for Immunology and Transfusion Medicine, in the group of Prof. Andreas Greinacher, University Medicine Greifswald, Germany. Here, she led two DFG grants, 3 year/each, and two startup grants supported by the Community Medicine and Molecular Medicine, University Medicine Greifswald, Germany. She also received a ‘Honor successful start-up financing projects in 2019’ from the Community Medicine and Molecular Medicine, University Medicine Greifswald, Germany. At Greifswald, she focused on a problem appeared in ~3% patients receiving heparin as anticoagulant which cause thrombocytopenia, the so called “Heparin-induced thrombocytopenia, HIT”. Since July 2019, Thi-Huong Nguyen has been leading the junior research group (NFG) at the IBA, Heiligenstadt, Germany. The NFG focuses on the role of HIT antibodies in human blood circulation. In particular, the group aims to i) develop a biosensor and improve enzyme immunoassays for better detection of HIT antibodies, ii) reduce platelet-surface activation by nanopatterning, and ii) investigate the interactions of HIT antibodies with cancer cells and platelets. The NFG composes of three PhD students and one technician. EXPERTISES She utilized biophysical methods to characterize and gain insights into molecular interactions of the complexes in human blood including HIT antibodies, platelets, heparins, cells, as well as DNA/aptamers, and magnetic nanoparticles. Single-molecule force spectroscopy and nanoindentation based Atomic force microscopy, Confocal laser scanning microscopy, Isothermal titration calorimetry, Dynamic light scattering, Circular dichroism spectroscopy, Surface plasma resonance, Antibody purification techniques, ELISA based assay, Cell culture, Preparation of human platelets from whole blood, Substrate and nanoparticle functionalization, Control of adhesion between platelets and solid surfaces were applied in her studies.
Transcript of Curriculum Vitae THI HUONG NGUYEN, PhD Contact · CV_____Dr. Thi Huong Nguyen 1 Curriculum Vitae...
CV_____Dr. Thi Huong Nguyen
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Curriculum Vitae THI HUONG NGUYEN, PhD
Contact Address Rosenhof 1, 37308 Heilbad Heiligenstadt, Germany
Tel +49 (0)3606 671 600 Fax +49 (0)3606 671 200 Email
Thi-Huong Nguyen studied bachelor and master’s degree in chemistry at the Hanoi University of Science, Vietnam. She worked at the Institute of Chemistry, Vietnamese Academy of Science and Technology, Vietnam on synthesizing the (17-a)- Ethynylestradiol (7/2000 - 2/2004). She moved to South Korea for her PhD study in Physics and Nanoscience, Department of Physics and Nanoscience, Sun Moon University South Korea(3/2004-8/2008). Her thesis focused on adhesion forces between l-DNA and different modified substrates. She then worked as a research assistant at the Nano/bio Research Center, Korea Institute of Science and Technology (KIST), Seoul, South Korea (10/2008-9/2009) on the projects enhancing accuracy of the elasticity measurement of a single double-strand DNA by atomic force microscopy and identifying the correlation of astrocyte stiffness with cytoskeletal maturation. She then studied her PhD in chemistry at the Max-Planck Institute for Polymer Research and Institute of Pharmacy and Biochemistry, the University of Mainz, Germany (10/2009-4/2013). Her thesis title is ‘Rupture Forces of Split Aptamers’. She joined the Nanostructure group at the Center for Innovation Competence for Humoral Immune Responses in Cardiovascular Disease (ZIK HIKE), University of Greifswald, Germany for 1.5 year postdoc. Her work focused on gaining insights into the interactions between platelet factor 4 and heparins of different lengths and interaction among platelets with different degrees of activation. Then, she moved to the Institute for Immunology and Transfusion Medicine, in the group of Prof. Andreas Greinacher, University Medicine Greifswald, Germany. Here, she led two DFG grants, 3 year/each, and two startup grants supported by the Community Medicine and Molecular Medicine, University Medicine Greifswald, Germany. She also received a ‘Honor successful start-up financing projects in 2019’ from the Community Medicine and Molecular Medicine, University Medicine Greifswald, Germany. At Greifswald, she focused on a problem appeared in ~3% patients receiving heparin as anticoagulant which cause thrombocytopenia, the so called “Heparin-induced thrombocytopenia, HIT”.
Since July 2019, Thi-Huong Nguyen has been leading the junior research group (NFG) at the IBA, Heiligenstadt, Germany. The NFG focuses on the role of HIT antibodies in human blood circulation. In particular, the group aims to i) develop a biosensor and improve enzyme immunoassays for better detection of HIT antibodies, ii) reduce platelet-surface activation by nanopatterning, and ii) investigate the interactions of HIT antibodies with cancer cells and platelets. The NFG composes of three PhD students and one technician.
EXPERTISES She utilized biophysical methods to characterize and gain insights into molecular interactions of the complexes in human blood including HIT antibodies, platelets, heparins, cells, as well as DNA/aptamers, and magnetic nanoparticles. Single-molecule force spectroscopy and nanoindentation based Atomic force microscopy, Confocal laser scanning microscopy, Isothermal titration calorimetry, Dynamic light scattering, Circular dichroism spectroscopy, Surface plasma resonance, Antibody purification techniques, ELISA based assay, Cell culture, Preparation of human platelets from whole blood, Substrate and nanoparticle functionalization, Control of adhesion between platelets and solid surfaces were applied in her studies.
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PUBLICATIONS (*Corresponding author) 1. Bui V.C., Gebicka P., Hippe H., Raschke R., Nguyen T.L., Greinacher A., Nguyen T.H.*,
Physicochemical Characteristics of Platelet Factor 4 under Various Conditions are Relevant for Heparin-Induced Thrombocytopenia Testing, J. Physical Chemistry (2020), doi.org/10.1021/acs.jpcb.9b11695.
2. Nguyen T.H.*, Xu Y., Brandt S., Mandelkow M., Raschke R., Strobel U, Delcea M., Zhou W., Liu J., and Greinacher A.*, Characterization of the Interaction between Platelet Factor 4 and Homogeneous Synthetic Low Molecular Weight Heparins, J Thromb Haemost. (2019) Oct 1. doi: 10.1111/jth.14657.
3. Nguyen T.H.*, Wesche J., Raschke R., Strobel U., Bui V.C., Delcea M. and Greinacher A.*, Reactivity of platelet-activating and nonplatelet-activating anti-PF4/heparin antibodies in enzyme immunosorbent assays under different conditions, J. Thromb Haemost, 17 (2019) 1113-1119.
4. Nguyen T.H.*, Greinacher A.*, Distinct Binding Characteristics of Pathogenic Anti-Platelet Factor-4/Polyanion Antibodies to Antigens Coated on Different Substrates: A Perspective on Clinical Application, ACS Nano, 12(12), 12030-12041 (2018).
5. Nguyen T.H.*, Schuster N., Greinacher A., Aurich K.*, Uptake pathways of protein-coated magnetic nanoparticles in platelets, ACS Applied Materials & Interfaces, 10 (34), 28314-21, (2018).
6. Bui V.C., Nguyen T.H.*, The Role of Single-Molecule Force Spectroscopy in Unraveling Typical and Autoimmune Heparin-induced Thrombocytopenia” Int. J. Mol. Sci., 19, 1054 (2018).doi: 10.3390/ijms19041054.
7. Bui V.C., Nguyen T.H.*, DNA aggregation induced by Mg2+ ions under different conditions, J Mol Recognit, (2018). DOI: 10.1002/jmr.2721.
8. Bui V.C., Nguyen T.H.*, Biophysical Characteristics of Hematopoietic Cells during Division, Exp Cell Res., 367(2),132-136 (2018).
9. Nguyen T.H.*, Medvedev N., Delcea M., Greinacher A.*, Anti-platelet factor 4/polyanion antibodies mediate a new mechanism of autoimmunity, Nature Communications 8:14945 (2017).
10. Nguyen T.H.*, Greinacher A.*, Platelet factor 4/Heparin complexes present differently in the purified system and on the platelet surface, Blood, (2017).
11. Nguyen T.H.*, Greinacher A., Effect of pH and Ionic Strength on the Binding Strength of PF4/Polyanion Antibodies, Eur Biophys J. (2017).
12. Bui V.C., Nguyen T.H.*, Insights into the interaction of CD4 with anti-CD4 antibodies, Immunobiology, 222(2), 148 (2017).
13. Nguyen T.H.*, Single Molecule Force Spectroscopy Applied to Heparin-Induced Thrombocytopenia, J Mol Recognit, 2585 (2017).
14. Henrich F., Fell D., Truszkowska D., Weirich M., Anyfantakis M., Nguyen T.H., M. Wagner, Auernhammer G.K.* and Butt H.J., Influence of surfactants in forced dynamic dewetting, Soft Matter 12(37), 7782 (2016).
15. Nguyen T.H.*, Palankar R., Bui V.C., Medvedev N., Greinacher A.*, and Delcea M.*. Rupture Forces among Human Blood Platelets at different Degrees of Activation, Scientific Reports 6,1-12 (2016).
16. Bui V.C., Nguyen T.H.*, The role of CD4 on mechanical properties of live cell membrane, J. Biomed. Mater. Res. A., 104(1), 239-44 (2016).
17. Nguyen T.H.*, Greinacher A., Delcea M.*, Quantitative description of thermodynamic and kinetic properties of the platelet factor 4/heparin bonds, Nanoscale 7, 10130-9 (2015).
18. Lee S. M., Nguyen T.H., Na K., Cho I.J., Woo D.H., Oh J.E., Lee C.J., Yoon E.S.*, Nanomechanical measurement of astrocyte stiffness correlated with cytoskeletal maturation, J. Biomed. Mater. Res. A., 103(1) 365-370 (2014).
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19. Eibach T.F., Fell D., Nguyen T.H., Butt H.J., Auernhammer G. K.*, Measuring contact angle and meniscus shape with a reflected laser beam, Rev. Sci. Instrum., 85, 13703-11 (2014).
20. Cui J.X., Nguyen T.H., Ceolin M., Berger R., Azzaroni O., del Campo A.*, Phototunable response in caged polymer brushes, Macromolecules, 45(7), 3213-3220 (2012).
21. Nguyen T.H., Steinbock L.J., Butt H.J., Helm M., Berger R.*, Measuring single small molecule binding via rupture forces of a split aptamer, J. Am. Chem. Soc., 133, 2025-2027 (2011).
22. Nguyen T.H., Lee S.M., Na K., Yang S., Kim J., Yoon E.S.*, An enhanced measurement of dsDNA elasticity using AFM, Nanotechnology, 21, 1-7 (2010).
23. Nguyen T.H., Kim Y.U., Choi S.S*, Investigation of structural transition of dsDNA on various substrates studied by atomic force microscopy, J. Nanosci. Nanotech. 9, 2162-2168 (2009).
24. Nguyen T.H., Bui V.C., Kim Y.N., Choi S.S.*, Tip functionalization by biotin linkers: a technique for DNA elasticity measurement studied by atomic force microscopy, J. Bionanosci., 3, 1-6 (2009).
25. Nguyen T.H., Bui V.C., Choi S.S. *, Manipulation of Single Molecule: DNA Physical Characteristic Observation Studied with Atomic Force Microscopy, Advances in Natural Sciences, 9, 225-232 (2008).
26. Nguyen T.H., Choi S.S., Kim Y.U., Kim D.W.*, Study on the binding force between λ-DNA and various types of substrates, J. Kor. Phys. Soc., 50, 1942-1946 (2007).
27. Luu D.H.*, Nguyen T.H., Study on Epimerization of the (17-a)- Ethynylestradiol. J. Chem. 5, 72-75 (2001).
28. Nguyen X.D., Nguyen V.N.*, Tran T.M.L., Le V.H., Nguyen T.H., Nguyen T.T., The use of gracilaria biomass for removal of heavy metals from aqueous solutions, J. Chem. 3, 89-91 (2001).
H-index: 10 (https://scholar.google.com/citations?user=4eV946UAAAAJ&hl=en)
PATTENTS Greinacher A, Nguyen T.H., Heparin-induced thrombocytopenia Assay, United States Patent, No. 20190346440
BOOK CHAPTERS Nguyen T.H.*, Not Only Heparin but Also Antibody Induces Thrombocytopenia. Book title: “Thrombocytopenia.” Edited by Pankaj Abrol, ISBN 978-953-51-3876-1, IntechOpen, DOI: 10.5772/intechopen.73576 (2018).
