CURRICULUM VITAE:

NAME: Damir Janigro BIRTH DATE: 3/16/1957 BIRTH PLACE: Zagreb, Croatia MARITAL STATUS: Married to Kim Ann Conklin, 2 children Mattia

and Alice Languages spoken: English, Italian, Serbian, and Croatian (perfect knowledge); German, French, Spanish at high school level. home: 2218 North St. James Parkway Cleveland Heights, Ohio 44106 email: djanigro@flocel.com Lapsus161@hotmail.com

Damir Janigro, Ph.D.

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CURRICULUM

1976-1982: Graduate student in the dept. of Physiology with Prof. D. DiFrancesco; voltage clamp

investigation of pacemaker activity in the heart. Ph.D. "cum laude"; thesis title: "The pacemaker current in heart Purkinje fibers"

1982-1984: Project Leader at FIDIA Research Laboratories, Abano Terme, Italy. histological and electrophysiological investigation of CNS neurons following trauma and during recovery. 1983: Visiting Scientist at the Karolinska Institutet, Stockholm. Immuno-

cytochemistry and HPLC analysis of monoaminergic pathways after neurochemical lesions 1984-1987: Postdoctoral Research Associate at the Dept. of Neurosurgery, Univ. of Washington with

P.A. Schwartzkroin; electrophysiology of the in vitro hippocampal slice: effects of anoxia 1987-1989: Research Associate in the Depts. of Pharmacology and Physiology at the University of Milan 1990-1995: Research Assistant Professor, Dept. of Neurosurgery, Univ. of Washington; 1993-1995: Research Assistant Professor (Adjunct), Dept. of Environmental Health, U of WA 1996-1999: Associate Professor of Neurological Surgery and Environmental Health, U of WA 1996-2002: Adjunct (Contract) Professor of Physiology, University of Milan, Italy 1999-2015: Full Staff, Cleveland Clinic Foundation, Cleveland, OH 1999-2015: Director, Cerebrovascular Research, Dept. of Neurosurgery, Cleveland Clinic 1999-2010: Affiliate Professor, Neurosurgery, University of Washington (Honorary title) 2002-2015: Graduate Faculty, Kent State University, School of Biomedical Sciences 2003-1012: Associate Staff, Brain Tumor Institute, Cleveland Clinic Foundation 2003-2015: Professor of Molecular Medicine, Cleveland Clinic Lerner College of Medicine 2003-2015: Adjunct Graduate faculty, Kent State University, Kent, OH 2005-2011: Associate staff, Swedish Hospital Neuroscience Institute, Seattle, WA 2008-2015: Scientific director, Music and Brain project, Arts and Medicine Institute Cleveland Clinic 2012-2015: Graduate Faculty, Adjunct Professor Cleveland State University 2012-present: External Associate, VIBRE at Vanderbilt Univ., Nashville Tennessee 2012-present: Member Neurobiology Commission International League Against Epilepsy 2015-present: Chief Scientific Officer Flocel, Inc. 2016-present: Founder, CEO and Chief Scientific Officer, FloTBI, Inc.

HONORS

1993: American Heart Association Young Investigator Award, Boston, MA 2001: NIH-NINDS Study Section NCD-II, Ad hoc 2001: NIH-NIAAA Study Section ZAAS, Member 2001-2005 AHA Study Section Brain 2, Member 2002-2005: NIH-NINDS Study Section NSD-C, Permanent Member 2004-2006: NIH Roadmap Review Group 2004-2010: Member NINDS/NIMH Microarray Consortium 2005: NIH NIAAA Study Section ZA11CC 2005-2010: Editorial Board, Epilepsy Currents 2006-2009: University of Texas Medical Branch, Galveston: Member of external scientific board for a

Program Grant (NINDS) (R. Perez-Polo). 2006: Member NIH ZDE1 GH (Natl. Inst. of Dental and Craniofacial Research) 2006: Reviewer for the Mariani Foundation, Italy 2006-2008: Reviewer for CURE (Citizen United for Research on Epilepsy 2007-: COBRE NIH-NCRR Study Section Member

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2007-present: Associated faculty Kent State University 2006-present: Associated faculty Seattle Neuroscience Institute 2005-present: CWRU Comprehensive Cancer Center Associate Member 2008-1010: Chairman of the American Heart Association BRAIN Study Section 2008: Co-Recipient of the Morris-Coole Prize for the “most significant advancement in knowledge

in the field of epilepsy” 2009: CURE study section reviewer 2008-: Ad hoc Member, study section BINP, NINDS CSR 2009: European commission HEALTH-2007-2.2.1-4 2010-2011: American Institute of Biological Sciences Epilepsy Research Panel Member 2011: Chairman, American Institute of Biological Sciences Epilepsy-Basic Research Panel 2012: Co-Organizer Gordon Conference on BBB and disease GRC Keynote speaker “Is there a BBB disease?” 2013-2015: Permanent Reviewer NIH BINP Study Section 2014-present: Member of the Neurobiology Commission, ILAE 2016: Elected Fellow of the American Epilepsy Society

SCIENTIFIC SOCIETIES

American Epilepsy Society, 1998-present Biophysical Society, 1994-2000 American Physiological Society, 1994-2004 International Brain Research Organization 1984-present American Society for Neuroscience, 1984-2005 American Heart Association, Stroke and Basic Science Council, 2001-2015

OTHER ACTIVITIES 2015-2016: Consultant for Diasorin, Inc. 2010-2015: Consultant for Numoda Consultant for Nichi-Bei Bio 2008: Board member A. Janigro Cello Competition (Zagreb, Croatia) 2007: Consultant for Fonds de Solidarité de la FTQ 545, Montreal, CA 2006 -2011 Consultant for the Seattle Neuroscience Institute, Swedish Hospital. Seattle, WA; Consultant for Heart-Brain Institute, Seattle Neuroscience Institute 2006 -2010 Organizer of Cello concertos in Cleveland and NE Ohio 2005 -present Founder of the Antonio Janigro Foundation (Ohio non-profit) 2005 -2010 Consultant for Abiogen, Inc. 2004 Co-founder of Flocel, Inc. Cleveland, OH 2004-2010: Consultant for Spencer Technologies Seattle, WA 2003: Co-founder Biosil, USA 2000- 2004: Consultant for IRB Italy 2000- 2003: Consultant for Sangtec, Inc. Sweden 1999-2000: Consultant for FiberCell, Inc. 1998-1999: Consultant for Berlex, Richmond, CA 1998-1999: Consultant for Epix Medical, Cambridge, MA 1997-1999: Consultant for Hedral Therapeutics, Portland, OR 1996-1998: Consultant for Prof. Jay Nelson, OHSU on Blood-brain barrier and HIV 1996-1997: Consultant for Parke-Davis, Ann Arbor, MI

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1997-1999: Consultant for Biosupport, Inc., Redmond, WA, 1995-1997: Consultant on blood-brain barrier models for Cellco Inc., Germantown, MD

TEACHING RESPONSIBILITIES 1985-1990: Lecturer at the post-graduate School of Physiology at the University of Milano. Taught basic

principles of electrophysiological techniques. 1988-1989: Lecturer in General Pharmacology at the University of Milano Medical School. Organizer the

b course and taught Cardiac Pharmacology.

Lecturer at the post-graduate School of Endocrinology at the University of Milano. Taught hypothalamic mechanisms in hormonal secretion.

1991-1992: Guest Lecturer in ENVH 531 Neurotoxicology, University of Washington. Taught application

of electrophysiological techniques to neurotoxicology. 1993-1998: ENVH 515. Lectured on Cardiac Toxicology

ENVH 531 Neurotoxicology, University of Washington (Blood-Brain Barrier in Neurotoxicology; Neurotoxicologic Techniques)

Lecturer: Neurosurgery Residents Seminar Series (Electrophysiological Techniques in Neuroscience)

1995: Freshman Seminars, General Studies 197D: “Brain Research, what is it and why do we

need it”. Lecturer in ENVH 515 (Cardiac Toxicology) 1996-1998: Lecturer in ENVH 515 (Cardiac Toxicology) 1999-2001: Lecturer for Residency training Program at The Cleveland Clinic Foundation (Neurology) 2003-2005: Medical School Curriculum Development Task Force, The Cleveland Clinic Foundation Lerner College of Medicine 2003-2005: Project Leader Neuroscience curriculum development. Lectures on CNS homeostasis, role

of CNS as integrator of peripheral functions 2004-2015: Medical School Curriculum Development (CCLCM; Neuroscience and Skeleto-muscular)

Medical School Curriculum Development Seminar organizer Weeks 4-5: “Neuronal communication. How the brain controls its own function”.

2004-2007: Post-doctoral Action Committee: “How are grants reviewed? Mock study sections for post-

docs and fellows” D.J. organizer and moderator: Seattle Neuroscience Institute, Seattle, WA 2007-2015: Cleveland Clinic Lerner College of Medicine admission committee 2010-2015: Seminar leader, Cleveland Clinic Lerner Graduate School Program The BBB in health and disease

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Control of brain homeostasis 2008-2010: Chairman “Study in Neurology” group (SIGN) at CCLCM financially supported by

the American Academy of Neurology 2009-2015: Leadership Group, Cerebrovascular Programmatic Research with Drs. Uchino and Rasmussen 2011-2012: Problem based learning Moderator, Renal and Neuroscience biology course Cleveland Clinic Lerner College of Medicine 2010-2014: Organized video link conferences with Harvard MGH Stroke group, Seattle Swedish Hospital, Stanford Neurosurgery and Duke

STUDENTS and FELLOWS PRECEPTED 2016 POST-DOCTORAL FELLOWS AND STUDENTS: Manoj, Banjara, PhD Aaron Dadas, BS Jolewis Washington, BS

RECENT GRADUATE-MEDICAL STUDENTS 2015-present: Briana Prager, MDc Medical Student Thesis Advisor and HHMI recipient 2015-present: Rebecca Achey, MDc Medical student and AAN Summer Research recipient 2012-present: Phil Iffland: PhD (KSU) Thesis advisor (post-doc in Dr. Peter Crino's lab, start Aug 2015) 2013: Thesis committee Maurine Cobabe, MDc 2012-present: Chanda Brennan, CSU Thesis advisor (graduates in 2015) 2010-2011: Thesis advisor Minh Nguyen, Honor thesis in Neuroscience (CWRU) 2011-2012: Erin Bargerstock MDc Cleveland Clinic Lerner College of Medicine, Chair of thesis committee and advisor (now at Univ. of Pittsburg, Pediatric Resident) 2009-10: Nancy Pham, MD Candidate Cleveland Clinic Lerner College of Medicine, Chair of thesis committee and advisor (now at Duke, Radiology Res. 2) 2007-2010: Catilin Perju, Ph.D. candidate, Kent State University (currently post-doctoral fellow at the Cleveland Clinic Foundation) 2007-2008 Ajush Batra, MD candidate Cleveland Clinic Lerner College of Medicine (currently a Howard Hughes Fellow at NINDS); DJ is Thesis committee chairman. HHMI recipient Sneha Ramakrishna, MD candidate Cleveland Clinic Lerner College of Medicine Mike Taylor, MD candidate (CWRU (now Neurology Resident George Washington Medical Center, Washington, DC) William Thierney, pre-Med CWRU (now MDc at CCLCM) Francesco Boselli, M Eng, University of Milan, Italy (now in UK) Chiara Caponi, University of Pisa, Italy AS EXTERNAL THESIS ADVISOR AT THE UNIV. OF MILANO, ITALY 1988-1990: J. Magistretti, Thesis advisor in a project entitled: "Electrophysiological properties of

prolactin secreting pituitary cells". (1999-present: Professor at Univ. of Pavia, Italy) 1987-1990: G. Maccaferri, M.D., Ph.D., University of Milano. Thesis advisor in a thesis project entitled:

"Calcium Currents in Rat Pheocromocytoma (PC12) cells"

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(1995: Post-Doctoral Fellow with C. McBain, NIH-NINDS;1997: Faculty in Oxford, UK; 2000, Currently Professor, Northwestern University, Chicago, IL)

AS EXTERNAL THESIS ADVISOR AT THE UNIV. OF PISA, ITALY 1996-1997: Eleonora Fornaciari, Ph.D. “In vitro modeling of the blood-brain barrier” (Fellow, Pharmacology, Univ. of Pisa; Private practice Pharmacist) 1996-1997: Patrizia Mascagni, Ph.D. “Drug permeation across the blood-brain barrier” (Currently Project Leader, Dompe Pharmaceutics) 1997-1998: Ilaria Luvisi, Ph.D. “Pharmacokinetics at the in vitro BBB” (Currently Scientific Research Manager, Procter & Gamble, Rome, Italy) 1999-2000: Luca Cucullo, Ph.D. “AZT exclusion at the BBB” (Currently Assistant Professor, U. Texas

Tech @ Amarillo) 2001-2004: Nicola Marchi, PhD now Tenured Faculty CNERN Montpellier. Recipient of the Morris-Coole Award 1999-2000: Francesco Macchia, Ph.D. “Glucose transport in an in vitro BBB model” 2002-2003: Giulia Betto, PhD “AED passage across the BBB” PRE-DOCTORAL FELLOWS, UNIVERSITY OF WASHINGTON, SEATTLE, WA 1995-1996: Regina Wenders, MD, PhD, DJ acted as Faculty Advisor for MSTP program, and Scientific

Advisor/Laboratory Instructor (Ph.D. thesis completed June 2000) 1993-1995: R. D’Ambrosio, Ph.D., University of Milano, Thesis advisor: “Complex effects of Cesium on

hippocampal neurons and astrocytes” (1998-present: Professor at the University of Washington)

1998-1999: Susan Leaman, MS candidate: “BBB permeability to antiretrovirals” (now Ph.D. student, Environmental Health, University of Washington)

1998: Christopher Anderson, “Adenosine transporters in the CNS” acted as External Examiner, Ph.D. program (Biochemistry) University of Manitoba, CA

1994-1995: Galen Hope Ransom, Undergraduate Student, DJ Faculty Advisor 1995-1997: Alfonsus Ngai, Ph.D. Postdoctoral Fellow, co-Advisor, DJ co-Sponsor for NIH Fellowship

(Present: Assistant Professor, Neurosurgery, University of Washington) 1994: David Lee Tinklepaugh, Graduate Student, DJ Faculty Advisor (Present: Resident in

Neurology, Univ. of VA) 1995-1996: Lauren Kronitz, Undergrad. Student Laboratory Training. POST-DOCTORAL FELLOWS: 2000-present: Q. Teng, MD now at Gainesville Florida Sheilesh, Desai, Ph.D.: Post-doctoral Fellow (now Project Scientist at CCF) Stefano Santaguida, PhD (now Mario Negri Milan) Shobu Namura, PhD (Professor, Morehouse SOM)

Ljiljana Bengez, MD, PhD: Post-doctoral Fellow (now Project Scientist) Marie Kelly-Leontiev: Post-doctoral Fellow (Associate Professor at Brigham Young) Miranda Kapural, M.D., Ph. D.: Post-doctoral Fellow (Now Anesthesia at CCF) Adriana Schwendtova, Ph.D.: Research Fellow

Tamer Attia, M.D.: Research Fellow (now Resident in Surgery, Univ. of Michigan)

Emil Zeynalov, M.D., Research Fellow Matteo Marroni, Ph.D., Post-doctoral Fellow (now Junior Faculty, Univ. of Pisa) Luca Cucullo, Ph.D., Post-doctoral Fellow (now Assistant Professor CCF-CWRU)

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Nicola Marchi, Ph.D., Post-doctoral Fellow (Assistant Professor Montpellier, France) Recipient of the Morris-Coole Award, 2007 Steve Dombrowski, Ph.D., Post-doctoral Fellow (now Project Scientist at CCF) Marc McAllister, M.D., Post-Med Research Fellow Gabriele Dini, Ph.D., Post-doctoral Fellow Said, Attia, M.D., Post-doctoral Fellow Said, Tamer, MD Post-doctoral Fellow Stefania Bassanini, PhD Post-doctoral Fellow (employed in industry) Chiara Marzotto, PhD Post-doctoral Fellow (Biosil USA) Sara Boninsegna, PhD Post-doctoral Fellow (Now Biosil USA)

Barbara Aumayr, MD, PhD candidate, University of Vienna John Michael Taylor, MD graduate CWRU Ezia Guatteo, Ph.D., Senior Fellow, DJ Mentor, 1994-1995 (Present: Associate Professor,

Univ. of Rome, Italy) Raimondo D’Ambrosio, Ph.D., Post-doctoral Fellow, DJ Mentor 1995-1998 (Present: Professor, Neurosurgery, University of Washington). Awarded American Epilepsy Society Award, 1998, NINDS RO1s, CURE grants etc. Adriana Emmi, Ph.D., Post-doctoral Fellow, DJ 1998-1999 (Present: Assistant Professor, Neurosurgery, University of Washington). Awarded American Epilepsy Society Award, 1999

MEDICAL RESIDENTS (RESEARCH TRAINING): G. Alex West, M.D.,Ph.D. Resident Faculty Advisor, 1990-1992 (1995-1999: Assistant Professor,

University of Texas, S. Antonio, TX; Associate Professor, Neurosurgery, University of Washington, Present: Professor of Neurosurgery, OHSU)

Guy McKhann, MD Resident Faculty Advisor, DJ NIH-NRSA; AANS Fellowship Mentor, 1995-1998 (Present: Associate Professor, Columbia University, New York, NY)

Gerry Grant, MD Resident Faculty Advisor, AHA Fellowship Mentor, 1996; NRSA co-Mentor, 1999) Now Assistant Professor at Stanford

Alain Barth, MD Acting Instructor, DJ Scientific Advisor 1994-96 (Present: Faculty, Univ. of Berne, Switzerland)

Donald Born M.D., Ph.D. Research Fellow, DJ Sponsor for NIH MCSDA (1995-1996) Presently at Stanford

MEDICAL/UNDERGRAD STUDENT EDUCATION CWRU-CCLCM 2000-2004 Erin Carlton, MDc Univ. of Toledo Nancy Pham, MDc CCLCM Barry Chisler, Med student (CWRU) Erin Ilkanich CWRU Master in Science Chiazor Ikbor CWRU Undergraduate Student Tom Rafferty, CWRU Undergraduate Student Michael Koltz, Medical College of Ohio, School of Medicine Erin Schmidt, Miami University Under graduate Student Gabrielle Ata, Miami University Under graduate Student Barbara Aumayr, University of Vienna, Molecular Biology Undergraduate Student

SCIENTIFIC PEER REVIEW COMMITTEES 1990-1993 Principal Reviewer “Alcohol and Drug Abuse Institute” (ADAI) University of Washington

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1992-present: Reviewer for: "Neurotoxicology", “Journal of Clinical Investigation” “Neurosurgery”, “American Journal of Physiology” “Journal of Neurophysiology”, “Neuroscience”, “Journal of Neuroscience”, “European Journal of Neuroscience” “Stroke”, “Science”, “Glia”, “Nature”, “Epilepsia”, “Science”, “Journal of Clinical Investigation”“JCBF”, “Brain Research” and other journals

2000-2003: Reviewer for the NIH, Lung, Blood, Heart Institute 1999-2001: Principal Reviewer for Canadian Medical Research Council Grant Applications 1999-2009: Principal Reviewer for EuroTelethon 1999-2009: Principal Reviewer Welcome Trust Research Fund (UK) 2001: Program Reviewer for Canadian Medical Research Council 2002-2005: American Heart Association, Member Peer Review Study Group-Brain 2 Section 2002-present: Reviewer, Welcome Trust, UK 2002-2003: Frontiers in Bioscience, Invited Editor 2004: Reviewer, Israeli Science Foundation 2004: Texas A&M University, Member of Dean’s Review Panel for Intramural Funding College

Station, Texas 2004: Canadian NRC – GHI Research Program Project, Grant Reviewer 2004: Italian Ministry for Education, University and Research: Reviewer 2004: NIH Roadmap Review Group II, Member 2002: NIH NIAAA Study Section ZA11CC 2004-2007: NIH-NINDS Clinical Neuroimmunology and Brain Tumors (CNBT) Study Section, Member 2000-2004: Member NIH-NINDS Study Section NCD-C 2005: NIH NIAAA Study Section ZA11CC 2005 Review for IOP Genomics 2006: Hong Kong University Grant Committee Reviewer 2006: Member NIH ZDE1 GH (Natl. Inst. of Dental and Craniofacial Research) 2006-present Reviewer for American Epilepsy Foundation 2007 NCRR COBRE Study Section 2006-present: University of Texas Medical Branch, Galveston: Member of external scientific board for a

Program Grant (NINDS) (R. Perez-Polo, PI). 2006: Member NIH ZDE1 GH (Natl. Inst. of Dental and Craniofacial Research) 2006: Reviewer for the Mariani Foundation, Italy 2006-2008: Reviewer for CURE (Citizen United for Research on Epilepsy 2007-2008: AHA Study Section Brain 3, Member 2007: Editorial Board Epilepsy Currents 2008: Editorial board IJ Developmental Neuroscience 2010-2011: Chairman, National American Heart Association Study Section Brain 1 2009-present: Member, or ad hoc BINP NIH NINDS Study Section 2009: Reviewer for Challenge-Omnibus NIH proposals 2011: Chairman PRMRP 2010: Epilepsy Panel 2011-present: Editorial board Epilepsia 2011-present: Editorial Board of Frontiers in Epilepsy 2014-present: Editorial Board PloS One 2013-present: Study section ZRG1 F03B-D Fellowship grants 2015-present: ZNS1 SRB-J (11) and ZNS1 SRB-N (11)

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CURRENT AND PAST FUNDING Pending R44NS098847-01 Janigro D. (mPI) 07/01/2017 to 06/30/2021 Point of care device for concussions

Total cost: $1,988,000

Active or recent 2R42MH093302 Janigro D. (PI) Development of a BBB Model to Study Transendothelial Cell Migration Phase II 09/27/2012 to 09/26/2016

Direct cost: $ 1,220,335 1R01NS078307 Janigro, mPI. (now consultant) Drug brain biotransformation in human refractory epilepsy 04/01/2012 to 03/31/2017

Direct cost: 1,250,000 Human Brain-on-a-Chip: Regional Chemical Communication, Drug and Toxin Responses (Janigro D. m-P; with Vanderbilt and Cleveland Clinic)

09/01/2012 to 08/31/2016 Direct cost: $ 900,000

R21NS077236 Janigro D. (PI) Serum markers of football-related brain concussions 09/01/2012 to 08/31/2014 Direct cost: $ 250,000 ELEKTA Janigro D. (PI) Peer reviewed research grant Markers of brain metastases 09/01/2012 to 08/31/2014 Direct cost: $ 110,000 Numoda Janigro D. (mPI) Sponsored study: VEGF in hemorrhagic conversion 09/01/2012 to 08/31/2015 Direct cost: $ 170,000

Biorepository Core Lab for the PTSD - TBI Clinical Consortium Duke University; Testing site for serum markers Cleveland Clinic (Cleveland Clinic P.I. Damir Janigro) 03/01/2015-02/28/2016 Direct cost $160,000

PAST GRANT PROPOSALS 1R41MH093302 Janigro D. (PI) 09/27/2010 to 09/26/2012 Development of a BBB Model to Study Transendothelial Cell Migration Phase I

Direct cost: $ 150,335

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R21NS077236 Janigro D. (PI) Serum markers of football-related brain concussions 09/01/2012 to 08/31/2016 Direct cost: $ 250,000 R21HD057256 Development of predictive in vitro model of drug absorption across the human BBB

2009-2011 Direct cost: $ 250,000

Cleveland Clinic-Swedish Satellite Laboratory Feasibility Project, Federal Appropriation WA State 2009-2010 Direct cost: $1,500,000

NIH/NINDS R01 D.J. P.I., Serum markers of brain barriers

2005-2011 Direct cost: $1,750,000

2008-2009: S-100ß as a Biochemical Surrogate of Mild Traumatic Brain Injury: A Prospective Pilot Study

Roche Diagnostics Grant in collaboration with Duke University Medical School; 3/01/08-2/28/09 PIs: Daniel Laskowicz, MD, Gerald Grant, MD, Damir Janigro

2005-2007: Measuring the human BBB Sponsor: Research Management Group Philip Morris $

338,405 2004-2009 :NIH/NINDS R01, (D.J. P.I. Shear Stress and Inflammation at the BBB, $1,912,000 2001-2006: NIH-NINDS R01 (D.J. PI) Mechanisms of multiple drug resistance at the BBB,

$2,450,000 2005-2009: A humanized model of the BBB to study cigarette smoke toxicity, Research Management

Group Philip Morris $ 777,350 (Luca Cucullo, PI DJ co-PI) 2006 Side-by-side comparison of BBB models NIH NINDS - STTR 2006-2007 (Phase I-II) P.I. of

the subcontract to CCF Damir Janigro ($1,251.526) 2004-2005: Biosil membrane in transplantation $287,000, Biosil USA 1999-2004: NIH/HLBI R01 D.J. P.I.,:, Regulation of Cerebral Blood Flow by ATP, $1,400,000 2002-2003: Biosil membrane in transplantation $177,000, I.R.B., Italy 1998-2003: NIH/NINDS R01 D.J. P.I.,, “Heterogeneity of hippocampal glia”, $841,742 1993-1998: NIH/HLBI, R29 FIRST Award: D.J. P.I., “Regulation of Cerebral Blood Flow by ATP” $400,000 1997-1998: Parke-Davis / UW: Permeation of PNQX across an in vitro BBB ($25,000) 1996-1997: NIEHS/NINDS Pilot Awards, “Effects of lead on the in vitro BBB” ($25,000) 1993-1994: NIH/NINDS CNS Trauma Project: Pilot and Feasibility Awards ($40,000) 1991-1993: American Heart Association (WA), Grant in Aid: Title: "Regulation of Cerebral Blood Flow

by ATP and Adenosine" ($40,000)

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AS CO-INVESTIGATOR: 2010: NIH NINDS RO1 (Luca Cucullo PI) Damir Janigro Co-PI (5%) “Comparative testing of

tobacco smoke toxicity at the blood-brain barrier 2005: A new DIV-BBB for extensive pharmacological study at the BBB; $ 40,000; 8/1/04 to

7/30/05. Sponsor: Alternatives Research & Development Foundation (ARDF) (PI: Luca Cucullo, PhD, Damir Janigro co-PI) $40,00

2002-2005: American Heart Association SDG The influence of shear stress on the BBB (Lily Bengez, MD PI, D. Janigro, co-PI) $260,000

2001-2002: National Center for Research Resources (NCRR) Shared Instrument Grant Quadruple-time-of-flight mass spectrometry system 1S10RR16794 (4/1/02-3/31/03) $430,460 (with M. Kinter, PI, Damir Janigro co-PI 10%)

2004: Bayer Pharmaceutical Corp. Damir Janigro, PhD, PI, $30,000, A Model of the Human Blood Brain Barrier Using Human Epithelial and Glia Cells

2000-2001: AHA-GIA Endothelial changes in Aneurysms, S. Desai, PI 2000-2004: NIH-RO1, “Effects of TBI in the hippocampus” (PI: MS Grady, 20%) 1994-1999: NIEHS, Center of Excellence, University of Washington, co-P.I. 1996-2001: NIH-RO1, (P.A. Schwartzkroin, P.I.; 10%) Title: Regulation of extracellular space in

epileptogenesis” 1997-2002: NIH RO1: Regulation of CBF by adenosine (HR Winn, P.I.; 25%) 1990-1996: NIH Program Project: H.R. Winn Principal Investigator. Title: "Influencing CNS Injury:

Primary & Secondary Factors" AS MENTOR: 2015: HHMI Research Fellowship, Briana Prager, MDc 2015: AAN Summer Research Program Rebecca Achey, MDc 2011-16: American Heart Association Scientist Development Grant (Chaitali Ghosh) 2008-2009: Howard Hughes Medical Investigation Cloisters Award (Ayush Batra, MDc) 2008: Morris-Coole Award Morris-Coole Prize for the “most significant advancement in

knowledge in the field of epilepsy” (recipient was the mentored Fellow, Dr. N. Marchi) 2003-2008: American Heart Association Scientist Development Grant (Lili Bengez) 2001-2003: NIH NRSA (S. Dombrowski, Ph.D.) Drug resistance in epileptogenesis 1999-2001: American Epilepsy Foundation Fellowship (Adriana Emmi, MD, Ph.D.) 1999-2001: American Epilepsy Foundation Junior Investigator Award (D’Ambrosio) 1999-2001 American Heart Association Fellowship to Dr. G.A. Grant 1996-1997 NIH NRSA (Guy McKhann, P.I.), Role of astrocytes in epileptogenesis 1996-1997 AANS (Guy McKhann, P.I.) Astrocytes in brain pathology AS CONSULTANT: 2010-2012: R21 Virginia Tech proposal on viral vector delivery across the BBB

2005: BIOSIL Project at CCF $267,936 2001-2004: S100β as marker of BBB function (Sangtec Medical, Sweden) 2001-2004: Safety of Biosil implants (with IRB Italy) 1998-1999: NIH-SBIR phase 1: (with Hedral Therapeutics, Portland, OR) “Delivery of antiretrovirals at

the blood-brain barrier” $120,000 1996-1999: Prof. Nelson’s (Dept. of Microbiology, OHSU) NIH R01:” HIV and Blood-brain barrier”

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MUSIC-RELATED ACTIVITIES

Founder of Antonio Janigro Foundation in NEO

Board member ChamberFest Cleveland Executive consulting director International A. Janigro Foundation

Board member of the International Antonio Janigro Foundation (Croatia)

Organizer of the International Antonio Janigro cello competition, with participants from all continent and 30 countries

Steering committee A. Janigro Chamber Music School for strings (Established 2012 in Zagreb)

Reviewer for classical music magazines online

Reviewer of modern literature for several online groups

Member of Cleveland Cello Society

Son’s Recollections of Antonio Janigro talk for the CCS

Organized travel and concert by Mr. Giovanni Sollima, Monika Leskovar and Umberto Clerici, to play for the Arts and Medicine program at the Heart Center

Established ad hoc string orchestra with CCLCM students and faculty; organized rehearsals and a public performance

Sponsor of the duo Caroline Goulding (violin) and Umberto Clerici (cello) and organizer of chamber music events in Cleveland. Three performances, one in the US, one in Europe and one in Canada in 2010

PATENTS ISSUED/FILED

US 6,667,172 Cell and tissue culture modeling device and apparatus and methods of using the same (Issued Dec 2003) US 09/957,023 A peripheral marker of blood-brain barrier permeability (Issued December, 2004) US 20040009581 Markers of blood barrier disruption and methods of using same (Issued July 2001) PCT/US2015/020561 DIAGNOSTIC MARKER FOR TREATMENT OF CEREBRAL ISCHEMIA Filed 2015 PCT/US12/37170 SERUM S100B AND USES THEREOF PCT Filed 2012 Serial No. 62/323,840 filed April 2016, ANTI-S100B AUTOANTIBODIES IN CEREBRAL SMALL VESSEL DISEASE CMD-095-PROV System and method for diagnosing diseased states using peripheral markers of blood-brain barrier disruption

EDITORIAL MEMBERSHIPS AND EDITORIAL COMMENTS

2004: Guest editor for Neurological Research 2007-present: Editorial board IJ Developmental Neuroscience 2005-2013: Editorial Board of Epilepsy Currents 2010-present: Editorial board Epilepsia 2009-present: Editorial Board of Frontiers in Epilepsy 2010-present: Advisory Board Member WebMed Central Neurology 2014-present: Editorial board PLOS one VEZZANI,A. and JANIGRO,D., Leukocyte-endothelial adhesion mechanisms in epilepsy: cheers and jeers, Epilepsy Curr., 9 (2009) 118-121

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JANIGRO D, AWASTHI S, AWASTHI YC, SHARMA R, YADAV S, SINGHAL SS, HALLENE K (2007) RLIP76 in AED drug resistance. Epilepsia 48: 1218-1219. JANIGRO, D., and Hallene, K., Blood-Brain-Barrier, hot water and seizures Neurological Sciences 2003 24(4):231. JANIGRO, D., Mayberg MR. Comment, Sahlein DH, Heyer EJ, et al. Failure of Introperative jugular bulb S100B and neuron-specific enolase sampling to predict cognitive injury after carotid endarterectomy. Neurosurgery 2003;53(6):1249 JANIGRO, D. Anaesthesia 2004 Apr;59(4):344-9. A response to 'Cerebral and extracerebral release of protein S100B in cardiac surgical patients', Snyder-Ramos SA, Gruhlke T, Bauer H, Bauer M, Luntz AP, Motsch J, Martin E, Vahl CF, Missler U, Wiesmann M and Bottiger BW, Anaesthesia 2004; 59: 344-9.

MEDIA COVERAGE

2003: Interview on Italian Channel 3 on “Brain drain in basic science and medicine” 2011: Interview for Washington DC Channel 9 2011: TV Interview on Fox News, ABC News and Channel 3 (e.g., http://www.youtube.com/watch?v=Gmem8L3fhpU) 2012: Radio interview on Ideastream NPR http://www.ideastream.org/news/feature/42440 Front page article in Plain Dealer 2012: Article in Plain Dealer on management of concussions

2013-2015 press and new coverage

DAVID EPSTEIN: STUDY: BLOOD TEST COULD DETECT BRAIN DAMAGE IN ACTIVE ATHLETES

SI.com - First, none of the players with elevated S100B in their blood had suffered a concussion, so sub-concussive hits were apparently all it took to undermine the integrity of the blood-brain barrier. (S100B blood tests are already used outside of sports, and as a ...

HITS TO HEAD MAY SPUR BRAIN-DAMAGING IMMUNE RESPONSE

Fox News -

Once open, a protein found in the brain called S100B can leak out. Because the body is not used to seeing

this protein in the blood, it may develop antibodies to it. The body produces antibodies to fight what it

perceives to be a harmful substance. If these...

EVEN MILD HITS TO HEAD DISRUPT BLOOD-BRAIN BARRIER

MedPage Today -

Leakage of the antigenic protein S100B from the brain into the serum -- indicating increased permeability

of the blood-brain barrier -- was significantly higher in players who had the highest number of

subconcussive hits to the head during a game, with a head ...

FOOTBALL INJURIES MAY TRIGGER HARMFUL IMMUNE SYSTEM RESPONSE

U.S. News & World Report -

Damir Janigro, Ph.D.

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"We looked at a group of football players from three different [college] football teams, and looked at the

blood for a certain brain protein called S100B," explained study co-author Dr. Jeffrey Bazarian, an

associate professor of emergency medicine at the ...

NEW RESEARCH POINTS TO BRAIN INJURIES IN COLLEGE FOOTBALL

Businessweek

As the National Football League continues to face scrutiny and litigation over concussions, new evidence

shows that brain injuries may be a problem at the collegiate level too. According to a study published today

in PLOS One, college football players.

NON-CONCUSSION FOOTBALL HEAD HITS CAN CAUSE BRAIN CHANGES, CLEVELAND CLINIC ...

Plain Dealer - Front page article

Normally, S100B isn't found elsewhere in the body in large amounts; it's confined by the "blood-brain

barrier," a unique filtering system that keeps harmful stuff such as toxins away from the vulnerable organ,

while retaining beneficial substances that keep the ...

Study: Football players can suffer brain changes without concussions

WKYC-TV -

For the blood test, researchers drew blood from football players at Baldwin Wallace University, John Carroll

University and the University of Rochester before and after games, in order to search for the S100B protein

in the blood. (Of the 67 players in the study, ...

How Football Hits Could Lead To An Immune Attack On The Brain

Popular Science -

Bazarian and his team found that at the same time there's all this S100B in the blood, the body also makes

antibodies against the protein, which is normally found surrounding the brain but not in the blood. "So it

looks like the body makes an antibody against a ...

Sports brain injury may actually be an autoimmune phenomenon

Science a Gogo -

Bazarian and his co-researchers found that S100B, a previously identified protein biomarker for traumatic

brain injury, was present in varying degrees in the blood samples of the 67 football players after every

game - even though none of them suffered a ...

Study: Non-concussive hits also impact brain

Fox Sports Ohio -

The study involved taking blood samples from football players at Baldwin Wallace University, John Carroll

University and the University of Rochester to measure the amount of the protein S100B in the blood before

and after games. Normally, S100B is only ...

STUDY FINDS HEAD INJURIES CAN TRIGGER AUTO-IMMUNE RESPONSES

KSN-TV -

CHARLOTTE, North Carolina (NBC) -- It's easy to imagine how blinding blows to the head can lead to

severe brain injury, but new research suggests that even minor hits may, over time, lead to long-term

problems. Doctors say repeated head trauma, like the ...

Concussions Could Lead to Severe Brain Injuries in Collegiate Football

TopNews United States - According to a study published today in PLOS One, football players at college level who usually have hard hits on their head can experience long-term brain damage despite the fact they are never concussed. Cleveland Clinic man uses music to promote health (Plain dealer, Cleveland OH, 2014) Immune response to injury may damage brain Times of India, 2014 Tackling Concussion Head On in the NFL Sports Medicine, 2014

Damir Janigro, Ph.D.

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New Role For Immune System In Epilepsy Onset Discovered Epilepsy Res. UK 2014 INVITED SPEAKER 2012-2016

Visiting scientist, Harvard SOM, Neuroradiology; Boston, MA April, 2014

Visiting professor, Stanford University Dept. of Neurological surgery, Palo Alto, CA February 12, 2014

Invited speaker, 2nd Annual Concussion Education Symposium, Cleveland Clinic, Beachwood, OH

Invited speaker, NCATS-NIH/FDA/DARPA Organs on a Chip meeting, Arlington, VA January 20, 2014

Invited speaker-4 oral presentations, International League for Epilepsy World meeting, Stockholm, Sweden June 2014,

Speaker, Kent State University, September 2014

Visiting professor and lecturer, Dept. of Neuroscience, Belo Horizonte, Brazil

Invited speaker, University Heights, OH Library system; Music and the brain in health and disease

Visiting professor and speaker January 2015, University of Louisville, Louisville, KY

Visiting professor and speaker February 2015, University of Kentucky, Lexington, KY

International BBB meeting, Israel 2012; Chair of one session and speaker at two sessions o Efficacy of anti-inflammatory therapy in a population of pediatric drug resistant epileptics o Are you in or out? Leukocyte diapedesis (or not) in seizure disorders

Gordon research conference; Keynote speaker (New Hampshire, 2012): o BBB in neurological disorders

University of Milan, Italy; Visiting professor; Physiology and Biophysics o Consequences of sub-concussion in athletes

Vanderbilt, Visiting professor and speaker; Dept. of BME o In vitro modeling of the human cerebrovascular unit

International Glia Symposium, Berlin, Germany; Invited speaker o Astrocytic proteins are markers of CNS disease

Univ. of Tennessee, Memphis; Visiting professor and speaker; Neuroscience o Seizures induced by BBB dysfunction

London, UK: European Epilepsy Meeting 2012, Invited speaker o BBB and epilepsy

Johnson and Johnson; Janssen Pharmaceuticals in Titusville, NJ o Music and brain: implication for art therapy

Albert Einstein College of Medicine; Dept. of Neurology; Invited speaker Consequences of sub-concussion in athletes

NIH-DARPA-FDA Combined meeting, Organ-on-a-chip; Alexandria, VA (Poster) o In vitro models of the BBB

4th London-Innsbruck Colloquium on Status Epilepticus in Salzburg, Austria o Anti-inflammatory therapy for SE

Visiting scientist, Department of Neurology, University of Pavia Medical Center, Pavia, Italy

Invited speaker, NCATS-NIH/FDA/DARPA Organs on a Chip meeting, Bethesda, MD

Visiting Scientist, Besta Neurological Institute, Milano, Italy

Invited speaker, Appleton, WI High School Music Program, (Music and the brain)

Invited speaker, CURE-sponsored Phil Schwartzkroin retirement conference, Pajaro Dunes Resort, Monterey, CA

Cerebrovascular Meeting, CVB Montreal 2013 (members of Lab presented)

Invited speaker, Society for Neuroscience International Meeting 2013, Brazil

Visiting professor, Epilepsy center, Albert Einstein College of Medicine, The Bronx, NY

Damir Janigro, Ph.D.

XVI

Visiting professor, Stanford University Dept. of Neurological surgery, Palo Alto, CA

Visiting scientist, Department of Neurology, University of Pavia Medical Center, Pavia, Italy

Other invited speaker (2009-2011)

Organizer: Cleveland Clinic Symposium “Role of leukocyte adhesion in central nervous system diseases”.

Invited speaker, Temple University, Philadelphia PA

Invited speaker, Vanderbilt Univ. Dept. of Biomedical engineering

Invited speaker (two oral communications) Meeting on Blood-Brain Barrier Dysfunction in Neurological Disorders, organized by the Academy of Sciences of Israel, Negev 2012 Campus for Desert Research at Ben Gurion University

-BBB dysfunction, seizures and epilepsy - what can patients tell us?

-Leukocytes in epilepsy

Invited speaker New York Academy of Sciences " Brain Barriers: A Hurdle for Drug Discovery” New York, NY Organized by the Biochemical Pharmacology Discussion Group and The New York Chapter of the American Chemical Society

Invited speaker 2012 Chicago Symposium on Translational Neuroscience

“Blood Brain Barrier in Health and Disease” University of Chicago

Invited speaker, 2nd Annual Cleveland Music and Medicine Symposium “The symphony of neurons”

Invited speaker, Biomarkers in diseases, Lund Sweden Diagnostic significance of serum markers of BBB function in neurological diseases

Invited speaker: Abu Dhabi Music & Art Festival (ADMAF), Music and the Brain

Invited speaker: Mozart & Science /Music in Medicine and Therapy 3rd International Congress “Music and intraoperative stress: The Cleveland Clinic experience.

Invited speaker, AES: Splenectomy modulates seizures

San Antonio, TX

Tobacco smoke toxicity at the blood-brain barrier. Gordon Research

Conference-Barriers of the CNS, New London, NH, June 20-25, 2010.

Visiting professor, Brain Research Institute, Zagreb Croatia

Invited speaker: Massachusetts General Hospital / MGH Seminar Series

Cleveland Clinic Foundation Arts and Medicine Symposium: Firing of sub-thalamic and thalamic neurons and musical experience

Cleveland Clinic Foundation ShowCase Seminar: The Neuroscience of music

CWRU MSTP Program - Guest Speaker CWRU, Neuroscience, Epilepsy and Music PUBLISHED WORK

1 Ghosh C, Hossain M, Solanki J, Najm I, Marchi N, Janigro D Overexpression of pregnane X and glucocorticoid receptors and the regulation of cytochrome P450 in human epileptic brain endothelial cells. Epilepsia. 2017 Feb 15. doi: 10.1111/epi.13703

2 Librizzi L, de Curtis M Janigro D, Runtz L, de Bock F, Barbier EL, Marchi N. Cerebrovascular heterogeneity and neuronal excitability Neurosci Lett. 2017 S0304-3940(17).

3 Dadas A, Washington J, Marchi N, Janigro D Improving the clinical management of traumatic brain injury through the pharmacokinetic modeling of peripheral blood biomarkers. Fluids Barriers CNS. 2016 Nov 30;13(1):21.

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4 Choi H; Puvenna V; Brennan C; Mahmoud S; Wang XF; Phillips M, Janigro D S100B and S100B autoantibody as biomarkers for early detection of brain metastases in lung cancer. Transl.Lung Cancer Res. 2016 Aug;5(4):413-9.

2 Walker LE; Janigro D; Heinemann U; Riikonen R; Bernard C; Patel M. WONOEP appraisal: Molecular and cellular biomarkers for epilepsy. Epilepsia 2016 Sep;57(9):1354-62.

3 Gulati G; Iffland PH; Janigro D; Zhang B; Luggen ME. Anti-NR2 antibodies, blood-brain barrier, and cognitive dysfunction. Clin Rheumatol. 2016 Jun 29.

4 Ghosh C; Hossain M; Solanki J; Dadas A; Marchi N; Janigro D. Pathophysiological implications of neurovascular P450 in brain disorders. Drug Discov.Today 2016 Jun 14.

5 Boussadia B; Gangarossa G; Mselli-Lakhal L; Rousset MC; de BF; Lassere F, Janigro D; Marchi, N. Lack of CAR impacts neuronal function and cerebrovascular integrity in vivo. Exp.Neurol. 2016 Sep;283(Pt A):39-48.

6 Zhang J; Puvenna V; Janigro D. Biomarkers of Traumatic Brain Injury and Their Relationship to Pathology. 2016.

7 Puvenna V; Engeler M; Banjara M; Brennan C; Schreiber P; Dadas A, Janigro D Is phosphorylated tau unique to chronic traumatic encephalopathy? Phosphorylated tau in epileptic brain and chronic traumatic encephalopathy. Brain Res. 2016 Jan 1;1630:225-40.

8 Glaser N; Lo W; Tancredi D; Orgain M; Puvenna V; Janigro D, Janigro D.Levels of S100B in brain and blood of rats with diabetic ketoacidosis. Brain Res. 2015 Oct 22;1624:536-44.

9 Marchi N; Banjara M; Janigro D. Blood-brain barrier, bulk flow, and interstitial clearance in epilepsy. J Neurosci.Methods 2016 Feb 15;260:118-24.

10 Falcone T; Janigro D; Lovell R; Simon B; Brown CA; Herrera M, Janigro D.S100B blood levels and childhood trauma in adolescent inpatients. J Psychiatr.Res. 2015 Mar;62:14-22.

11 Ghosh C; Hossain M; Spriggs A; Ghosh A; Grant GA; Marchi N, Janigro D Sertraline-induced potentiation of the CYP3A4-dependent neurotoxicity of carbamazepine: an in vitro study. Epilepsia 2015 Mar;56(3):439-49.

12 Smith ES; Jonason A; Reilly C; Veeraraghavan J; Fisher T; Doherty M, Janigro D.SEMA4D compromises blood-brain barrier, activates microglia, and inhibits remyelination in neurodegenerative disease. Neurobiol.Dis. 2015 Jan;73:254-68.

13 Boussadia B; Ghosh C; Plaud C; Pascussi JM; de BF; Rousset MC, Janigro D; Marchi, N. Effect of status epilepticus and antiepileptic drugs on CYP2E1 brain expression. Neuroscience 2014 Dec 5;281:124-34.

14 Wathen C; Janigro D. IL-1beta associations with posttraumatic epilepsy development: a genetics and biomarker cohort study. Epilepsia 2014 Aug;55(8):1313.

15 Janigro D; Walker MC. What non-neuronal mechanisms should be studied to understand epileptic seizures? Adv.Exp.Med.Biol. 2014;813:253-64.

16 Bargerstock E; Puvenna V; Iffland P; Falcone T; Hossain M; Vetter S, Janigro, D. Is peripheral immunity regulated by blood-brain barrier permeability changes? PLoS.One. 2014;9(7):e101477.

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17 Puvenna V; Brennan C; Shaw G; Yang C; Marchi N; Bazarian JJ, Janigro D; Significance of ubiquitin carboxy-terminal hydrolase L1 elevations in athletes after sub-concussive head hits. PLoS.One. 2014;9(5):e96296.

18 Bazarian JJ; Zhu T; Zhong J; Janigro D; Rozen E; Roberts A, Janigro D.Persistent, long-term cerebral white matter changes after sports-related repetitive head impacts. PLoS.One. 2014;9(4):e94734.

19 Marchi N; Granata T; Janigro D. Inflammatory pathways of seizure disorders. Trends Neurosci. 2014 Feb;37(2):55-65.

20 Guo K; Janigro D. New immunological approaches in treating and diagnosing CNS diseases. Pharm.Pat Anal. 2013 May;2(3):361-71.

21 Janigro D; Iffland PH; Marchi N; Granata T. A role for inflammation in status epilepticus is revealed by a review of current therapeutic approaches. Epilepsia 2013 Sep;54 Suppl 6:30-2.

22 Iffland PH; Carvalho-Tavares J; Trigunaite A; Man S; Rasmussen P; Alexopoulos A, Janigro D; Intracellular and circulating neuronal antinuclear antibodies in human epilepsy. Neurobiol.Dis. 2013 Nov;59:206-19.

23 Ghosh C; Hossain M; Puvenna V; Martinez-Gonzalez J; Alexopolous A; Janigro D. Expression and functional relevance of UGT1A4 in a cohort of human drug-resistant epileptic brains. Epilepsia 2013 Sep;54(9):1562-70.

24 Falcone T; Carlton E; Lee C; Janigro M; Fazio V; Forcen FE, Janigro D. Does Systemic Inflammation Play a Role in Pediatric Psychosis? Clin Schizophr.Relat Psychoses. 2015;9(2):65-78B.

25 Marchi N; Bazarian JJ; Puvenna V; Janigro M; Ghosh C; Zhong J, Janigro D. Consequences of repeated blood-brain barrier disruption in football players. PLoS.One. 2013;8(3):e56805.

26 Cucullo L; Hossain M; Tierney W; Janigro D. A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box. BMC.Neurosci. 2013;14:18.

27 de Vries HE; Kooij G; Frenkel D; Georgopoulos S; Monsonego A; Janigro D. Inflammatory events at blood-brain barrier in neuroinflammatory and neurodegenerative disorders: implications for clinical disease. Epilepsia 2012 Nov;53 Suppl 6:45-52.

28 Marchi N; Granata T; Ghosh C; Janigro D. Blood-brain barrier dysfunction and epilepsy: pathophysiologic role and therapeutic approaches. Epilepsia 2012 Nov;53(11):1877-86.

29 Janigro D. Are you in or out? Leukocyte, ion, and neurotransmitter permeability across the epileptic blood-brain barrier. Epilepsia 2012 Jun;53 Suppl 1:26-34.

30 Sarkis RA; Jehi L; Silveira D; Janigro D; Najm I. Patients with generalised epilepsy have a higher white blood cell count than patients with focal epilepsy. Epileptic.Disord. 2012 Mar;14(1):57-63.

31 Ghosh C; Marchi N; Hossain M; Rasmussen P; Alexopoulos AV; Gonzalez-Martinez J, Janigro D A pro-convulsive carbamazepine metabolite: quinolinic acid in drug resistant epileptic human brain. Neurobiol.Dis. 2012 Jun;46(3):692-700.

32 Marchi N; Granata T; Alexopoulos A; Janigro D. The blood-brain barrier hypothesis in drug resistant epilepsy. Brain 2012 Apr;135(Pt 4):e211.

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33 Yuan Q; Fu Y; Kao WJ; Janigro D; Yang H. Transbuccal Delivery of CNS Therapeutic Nanoparticles: Synthesis, Characterization, and In Vitro Permeation Studies. ACS Chem.Neurosci. 2011 Nov 16;2(11):676-83.

34 Biddlestone-Thorpe L; Marchi N; Guo K; Ghosh C; Janigro D; Valerie K, Janigro D.Nanomaterial-mediated CNS delivery of diagnostic and therapeutic agents. Adv.Drug Deliv.Rev. 2012 May 15;64(7):605-13.

35 Marchi N; Johnson AJ; Puvenna V; Johnson HL; Tierney W; Ghosh C, Janigro, D. Modulation of peripheral cytotoxic cells and ictogenesis in a model of seizures. Epilepsia 2011 Sep;52(9):1627-34.

36 Cucullo L; Hossain M; Puvenna V; Marchi N; Janigro D. The role of shear stress in Blood-Brain Barrier endothelial physiology. BMC.Neurosci. 2011;12:40.

37 Ghosh C; Puvenna V; Gonzalez-Martinez J; Janigro D; Marchi N. Blood-brain barrier P450 enzymes and multidrug transporters in drug resistance: a synergistic role in neurological diseases. Curr.Drug Metab 2011 Oct;12(8):742-9.

38 Marchi N; Tierney W; Alexopoulos AV; Puvenna V; Granata T; Janigro D. The etiological role of blood-brain barrier dysfunction in seizure disorders. Cardiovasc.Psychiatry Neurol. 2011;2011:482415.

39 Marchi N; Granata T; Freri E; Ciusani E; Ragona F; Puvenna V, Janigro D. Efficacy of anti-inflammatory therapy in a model of acute seizures and in a population of pediatric drug resistant epileptics. PLoS.One. 2011;6(3):e18200.

40 Neuwelt EA; Bauer B; Fahlke C; Fricker G; Iadecola C; Janigro D, Janigro D.Engaging neuroscience to advance translational research in brain barrier biology. Nat.Rev.Neurosci. 2011 Mar;12(3):169-82.

41 Mazzone P; Tierney W; Hossain M; Puvenna V; Janigro D; Cucullo L. Pathophysiological impact of cigarette smoke exposure on the cerebrovascular system with a focus on the blood-brain barrier: expanding the awareness of smoking toxicity in an underappreciated area. Int.J Environ.Res.Public Health 2010 Dec;7(12):4111-26.

42 Ghosh C; Marchi N; Desai NK; Puvenna V; Hossain M; Gonzalez-Martinez J, Janigro D. Cellular localization and functional significance of CYP3A4 in the human epileptic brain. Epilepsia 2011 Mar;52(3):562-71.

43 Pham N; Fazio V; Cucullo L; Teng Q; Biberthaler P; Bazarian JJ, Janigro D. Extracranial sources of S100B do not affect serum levels. PLoS.One. 2010;5(9).

44 Cucullo L; Marchi N; Hossain M; Janigro D. A dynamic in vitro BBB model for the study of immune cell trafficking into the central nervous system. J Cereb.Blood Flow Metab 2011 Feb;31(2):767-77.

45 Falcone T; Mishra L; Carlton E; Lee C; Butler RS; Janigro D, Janigro D.Suicidal behavior in adolescents with first-episode psychosis. Clin Schizophr.Relat Psychoses. 2010 Apr;4(1):34-40.

46 Marchi N; Teng Q; Ghosh C; Fan Q; Nguyen MT; Desai NK, Janigro D. Blood-brain barrier damage, but not parenchymal white blood cells, is a hallmark of seizure activity. Brain Res. 2010 Sep 24;1353:176-86.

47 Falcone T; Fazio V; Lee C; Simon B; Franco K; Marchi N, Janigro D. Serum S100B: a potential biomarker for suicidality in adolescents? PLoS.One. 2010;5(6):e11089.

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48 Janigro D. Not again! The role of blood-brain barrier failure in epileptogenesis: a molecular update. Epilepsy Curr. 2010 May;10(3):67-9.

49 Marchi N; Teng Q; Nguyen MT; Franic L; Desai NK; Masaryk T, Janigro D. Multimodal investigations of trans-endothelial cell trafficking under condition of disrupted blood-brain barrier integrity. BMC.Neurosci. 2010;11:34.

50 Ghosh C; Gonzalez-Martinez J; Hossain M; Cucullo L; Fazio V; Janigro D, Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow. Epilepsia 2010 Aug;51(8):1408-17.

51 Marchi N; Gonzalez-Martinez J; Nguyen MT; Granata T; Janigro D. Transporters in drug-refractory epilepsy: clinical significance. Clin Pharmacol.Ther. 2010 Jan;87(1):13-5.

52 Granata T; Marchi N; Carlton E; Ghosh C; Gonzalez-Martinez J; Alexopoulos AV, Janigro D. Management of the patient with medically refractory epilepsy. Expert.Rev.Neurother. 2009 Dec;9(12):1791-802.

53 Mazzone PJ; Marchi N; Fazio V; Taylor JM; Masaryk T; Bury L, Janigro D. Small vessel ischemic disease of the brain and brain metastases in lung cancer patients. PLoS.One. 2009;4(9):e7242.

54 Vezzani A; Janigro D. Leukocyte-endothelial adhesion mechanisms in epilepsy: cheers and jeers. Epilepsy Curr. 2009 Jul;9(4):118-21.

55 Wang G; Guo Q; Hossain M; Fazio V; Zeynalov E; Janigro D Bone marrow-derived cells are the major source of MMP-9 contributing to blood-brain barrier dysfunction and infarct formation after ischemic stroke in mice. Brain Res. 2009 Oct 19;1294:183-92.

56 Hossain M; Sathe T; Fazio V; Mazzone P; Weksler B; Janigro D. Tobacco smoke: a critical etiological factor for vascular impairment at the blood-brain barrier. Brain Res. 2009 Sep 1;1287:192-205.

57 Buhler LA; Samara R; Guzman E; Wilson CL; Krizanac-Bengez L; Janigro D. Matrix metalloproteinase-7 facilitates immune access to the CNS in experimental autoimmune encephalomyelitis. BMC.Neurosci. 2009;10:17.

58 Marchi N; Betto G; Fazio V; Fan Q; Ghosh C; Machado A, Janigro D. Blood-brain barrier damage and brain penetration of antiepileptic drugs: role of serum proteins and brain edema. Epilepsia 2009 Apr;50(4):664-77.

59 Marchi N; Fan Q; Ghosh C; Fazio V; Bertolini F; Betto G, Janigro D. Antagonism of peripheral inflammation reduces the severity of status epilepticus. Neurobiol.Dis. 2009 Feb;33(2):171-81.

60 Fan QY; Ramakrishna S; Marchi N; Fazio V; Hallene K; Janigro D. Combined effects of prenatal inhibition of vasculogenesis and neurogenesis on rat brain development. Neurobiol.Dis. 2008 Dec;32(3):499-509.

61 Chapin JS; Busch RM; Janigro D; Dougherty M; Tilelli CQ; Lineweaver TT APOE epsilon4 is associated with postictal confusion in patients with medically refractory temporal lobe epilepsy. Epilepsy Res. 2008 Oct;81(2-3):220-4.

62 Falcone T; Carlton E; Janigro D; Simon B; Franco K. Self-harm during first-episode psychosis. Br.J Psychiatry 2008 Aug;193(2):167.

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63 Janigro D. Gene expression in temporal lobe epilepsy. Epilepsy Curr. 2008 Jan;8(1):23-4.

64 Marchi N; Mazzone P; Fazio V; Mekhail T; Masaryk T; Janigro D. ProApolipoprotein A1: a serum marker of brain metastases in lung cancer patients. Cancer 2008 Mar 15;112(6):1313-24.

65 Czeisler BM; Janigro D. Reading and writing the blood-brain barrier: relevance to therapeutics. Recent Pat CNS.Drug Discov. 2006 Jun;1(2):157-73.

66 Uva L; Librizzi L; Marchi N; Noe F; Bongiovanni R; Vezzani A, Janigro D. Acute induction of epileptiform discharges by pilocarpine in the in vitro isolated guinea-pig brain requires enhancement of blood-brain barrier permeability. Neuroscience 2008 Jan 2;151(1):303-12.

67 Janigro D. Does leakage of the blood-brain barrier mediate epileptogenesis? Epilepsy Curr. 2007 Jul;7(4):105-7.

68 Marchi N; Oby E; Batra A; Uva L; de CM; Hernandez N, Janigro D.In vivo and in vitro effects of pilocarpine: relevance to ictogenesis. Epilepsia 2007 Oct;48(10):1934-46.

69 Cucullo L; Couraud PO; Weksler B; Romero IA; Hossain M; Rapp E, Janigro D. Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies. J Cereb.Blood Flow Metab 2008 Feb;28(2):312-28.

70 Janigro D; Awasthi S; Awasthi YC; Sharma R; Yadav S; Singhal SS, Janigro D. RLIP76 in AED drug resistance. Epilepsia 2007 Jun;48(6):1218-9.

71 Janigro D. Brain water and ion fluxes: a hard-to-die hypothesis to explain seizures. Epilepsy Curr. 2007 Mar;7(2):56-7.

72 Bassanini S; Hallene K; Battaglia G; Finardi A; Santaguida S; Cipolla M, Janigro D. Early cerebrovascular and parenchymal events following prenatal exposure to the putative neurotoxin methylazoxymethanol. Neurobiol.Dis. 2007 May;26(2):481-95.

73 Cucullo L; Hossain M; Rapp E; Manders T; Marchi N; Janigro D. Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic drugs. Epilepsia 2007 Mar;48(3):505-16.

74 Marchi N; Angelov L; Masaryk T; Fazio V; Granata T; Hernandez N, Janigro D. Seizure-promoting effect of blood-brain barrier disruption. Epilepsia 2007 Apr;48(4):732-42.

75 Kellinghaus C; Moddel G; Shigeto H; Ying Z; Jacobsson B; Gonzalez-Martinez J, Janigro D. Dissociation between in vitro and in vivo epileptogenicity in a rat model of cortical dysplasia. Epileptic.Disord. 2007 Mar;9(1):11-9.

76 Janigro D. Inhibitory GABA current rundown in epileptic brain: use-dependent and pathology-specific mechanisms. Epilepsy Curr. 2006 Jul;6(4):138-9.

77 Oby E; Janigro D. The blood-brain barrier and epilepsy. Epilepsia 2006 Nov;47(11):1761-74.

78 Hallene KL; Oby E; Lee BJ; Santaguida S; Bassanini S; Cipolla M, Janigro D. Prenatal exposure to thalidomide, altered vasculogenesis, and CNS malformations. Neuroscience 2006 Sep 29;142(1):267-83.

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79 Santaguida S; Janigro D; Hossain M; Oby E; Rapp E; Cucullo L. Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: a permeability study. Brain Res. 2006 Sep 13;1109(1):1-13.

80 Krizanac-Bengez L; Hossain M; Fazio V; Mayberg M; Janigro D. Loss of flow induces leukocyte-mediated MMP/TIMP imbalance in dynamic in vitro blood-brain barrier model: role of pro-inflammatory cytokines. Am J Physiol Cell Physiol 2006 Oct;291(4):C740-C749.

81 Oby E; Caccia S; Vezzani A; Moeddel G; Hallene K; Guiso G, Janigro D.In vitro responsiveness of human-drug-resistant tissue to antiepileptic drugs: insights into the mechanisms of pharmacoresistance. Brain Res. 2006 May 1;1086(1):201-13.

82 Janigro D; Perju C; Fazio V; Hallene K; Dini G; Agarwal MK, Janigro D. Alternating current electrical stimulation enhanced chemotherapy: a novel strategy to bypass multidrug resistance in tumor cells. BMC.Cancer 2006;6:72.

83 Marchi N; Guiso G; Rizzi M; Pirker S; Novak K; Czech T, Janigro D. A pilot study on brain-to-plasma partition of 10,11-dyhydro-10-hydroxy-5H-dibenzo(b,f)azepine-5-carboxamide and MDR1 brain expression in epilepsy patients not responding to oxcarbazepine. Epilepsia 2005 Oct;46(10):1613-9.

84 Awasthi S; Hallene KL; Fazio V; Singhal SS; Cucullo L; Awasthi YC, Janigro D. RLIP76, a non-ABC transporter, and drug resistance in epilepsy. BMC.Neurosci. 2005;6:61.

85 Vogelbaum MA; Masaryk T; Mazzone P; Mekhail T; Fazio V; McCartney S, Janigro D. S100beta as a predictor of brain metastases: brain versus cerebrovascular damage. Cancer 2005 Aug 15;104(4):817-24.

86 Krizanac-Bengez L; Mayberg MR; Cunningham E; Hossain M; Ponnampalam S; Parkinson FE, Janigro D. Loss of shear stress induces leukocyte-mediated cytokine release and blood-brain barrier failure in dynamic in vitro blood-brain barrier model. J Cell Physiol 2006 Jan;206(1):68-77.

87 Moddel G; Jacobson B; Ying Z; Janigro D; Bingaman W; Gonzalez-Martinez J, Janigro D. The NMDA receptor NR2B subunit contributes to epileptogenesis in human cortical dysplasia. Brain Res. 2005 Jun 7;1046(1-2):10-23.

88 Cucullo L; Dini G; Hallene KL; Fazio V; Ilkanich EV; Igboechi C, Janigro D. Very low intensity alternating current decreases cell proliferation. Glia 2005 Jul;51(1):65-72.

89 Krizanac-Bengez L; Mayberg MR; Janigro D. The cerebral vasculature as a therapeutic target for neurological disorders and the role of shear stress in vascular homeostatis and pathophysiology. Neurol.Res. 2004 Dec;26(8):846-53.

90 Cucullo L; Aumayr B; Rapp E; Janigro D. Drug delivery and in vitro models of the blood-brain barrier. Curr.Opin.Drug Discov.Devel. 2005 Jan;8(1):89-99.

91 Albensi BC; Igoechi C; Janigro D; Ilkanich E. Why do many NMDA antagonists fail, while others are safe and effective at blocking excitotoxicity associated with dementia and acute injury? Am J Alzheimers.Dis.Other Demen. 2004 Sep;19(5):269-74.

92 Janigro D. A response to 'Cerebral and extracerebral release of protein S100B in cardiac surgical patients', Snyder-Ramos SA, Gruhlke T, Bauer H, Bauer M, Luntz AP, Motsch J, Martin E, Vahl CF, Missler U, Wiesmann M and Bottiger BW, Anaesthesia 2004; 59: 344-9. Anaesthesia 2004 Nov;59(11):1149-50.

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93 Marchi N; Hallene KL; Kight KM; Cucullo L; Moddel G; Bingaman W, Janigro D. Significance of MDR1 and multiple drug resistance in refractory human epileptic brain. BMC.Med. 2004 Oct 9;2:37.

94 Fazio V; Bhudia SK; Marchi N; Aumayr B; Janigro D. Peripheral detection of S100beta during cardiothoracic surgery: what are we really measuring? Ann.Thorac.Surg. 2004 Jul;78(1):46-52.

95 Marchi N; Cavaglia M; Fazio V; Bhudia S; Hallene K; Janigro D. Peripheral markers of blood-brain barrier damage. Clin Chim.Acta 2004 Apr;342(1-2):1-12.

96 Albensi BC; Ata G; Schmidt E; Waterman JD; Janigro D. Activation of long-term synaptic plasticity causes suppression of epileptiform activity in rat hippocampal slices. Brain Res. 2004 Feb 13;998(1):56-64.

97 Cucullo L; Hallene K; Dini G; Dal TR; Janigro D. Glycerophosphoinositol and dexamethasone improve transendothelial electrical resistance in an in vitro study of the blood-brain barrier. Brain Res. 2004 Feb 6;997(2):147-51.

98 Janigro D; Hallene K. Blood-brain barrier, hot water and seizures. Neurol.Sci. 2003 Nov;24(4):231.

99 Marroni M; Agrawal ML; Kight K; Hallene KL; Hossain M; Cucullo L, Janigro D. Relationship between expression of multiple drug resistance proteins and p53 tumor suppressor gene proteins in human brain astrocytes. Neuroscience 2003;121(3):605-17.

100 Marchi N; Rasmussen P; Kapural M; Fazio V; Kight K; Mayberg MR, Janigro D. Peripheral markers of brain damage and blood-brain barrier dysfunction. Restor.Neurol.Neurosci. 2003;21(3-4):109-21.

101 Marroni M; Kight KM; Hossain M; Cucullo L; Desai SY; Janigro D. Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis. Methods Mol.Med. 2003;89:419-34.

102 Stenina OI; Desai SY; Krukovets I; Kight K; Janigro D; Topol EJ, Janigro D. Thrombospondin-4 and its variants: expression and differential effects on endothelial cells. Circulation 2003 Sep 23;108(12):1514-9.

103 Parkinson FE; Friesen J; Krizanac-Bengez L; Janigro D. Use of a three-dimensional in vitro model of the rat blood-brain barrier to assay nucleoside efflux from brain. Brain Res. 2003 Aug 8;980(2):233-41.

104 Albensi BC; Janigro D. Traumatic brain injury and its effects on synaptic plasticity. Brain Inj. 2003 Aug;17(8):653-63.

105 Krizanac-Bengez L; Kapural M; Parkinson F; Cucullo L; Hossain M; Mayberg MR, Janigro D. Effects of transient loss of shear stress on blood-brain barrier endothelium: role of nitric oxide and IL-6. Brain Res. 2003 Jul 11;977(2):239-46.

106 Kanner AA; Marchi N; Fazio V; Mayberg MR; Koltz MT; Siomin V, Janigro D. Serum S100beta: a noninvasive marker of blood-brain barrier function and brain lesions. Cancer 2003 Jun 1;97(11):2806-13.

107 Cucullo L; Marchi N; Marroni M; Fazio V; Namura S; Janigro D. Blood-brain barrier damage induces release of alpha2-macroglobulin. Mol.Cell Proteomics. 2003 Apr;2(4):234-41.

108 Marroni M; Marchi N; Cucullo L; Abbott NJ; Signorelli K; Janigro D. Vascular and parenchymal mechanisms in multiple drug resistance: a lesson from human epilepsy. Curr.Drug Targets. 2003 May;4(4):297-304.

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109 Grant GA; Meno JR; Nguyen TS; Stanness KA; Janigro D; Winn RH. Adenosine-induced modulation of excitatory amino acid transport across isolated brain arterioles. J Neurosurg. 2003 Mar;98(3):554-60.

110 Marchi N; Fazio V; Cucullo L; Kight K; Masaryk T; Barnett G, Janigro D. Serum transthyretin monomer as a possible marker of blood-to-CSF barrier disruption. J Neurosci. 2003 Mar 1;23(5):1949-55.

111 Cucullo L; McAllister MS; Kight K; Krizanac-Bengez L; Marroni M; Mayberg MR, Janigro D. A new dynamic in vitro model for the multidimensional study of astrocyte-endothelial cell interactions at the blood-brain barrier. Brain Res. 2002 Oct 4;951(2):243-54.

112 Desai SY; Marroni M; Cucullo L; Krizanac-Bengez L; Mayberg MR; Hossain MT, Janigro D. Mechanisms of endothelial survival under shear stress. Endothelium 2002;9(2):89-102.

113 Strelow L; Janigro D; Nelson JA. Persistent SIV infection of a blood-brain barrier model. J Neurovirol. 2002 Aug;8(4):270-80.

114 Salvetti F; Cecchetti P; Janigro D; Lucacchini A; Benzi L; Martini C. Insulin permeability across an in vitro dynamic model of endothelium. Pharm.Res. 2002 Apr;19(4):445-50.

115 Kapural M; Krizanac-Bengez L; Barnett G; Perl J; Masaryk T; Apollo D, Janigro D. Serum S-100beta as a possible marker of blood-brain barrier disruption. Brain Res. 2002 Jun 14;940(1-2):102-4.

116 Abbott NJ; Khan EU; Rollinson CM; Reichel A; Janigro D; Dombrowski SM. Drug resistance in epilepsy: the role of the blood-brain barrier. Novartis.Found.Symp. 2002;243:38-47.

117 Dombrowski SM; Desai SY; Marroni M; Cucullo L; Goodrich K; Bingaman W, Janigro D. Overexpression of multiple drug resistance genes in endothelial cells from patients with refractory epilepsy. Epilepsia 2001 Dec;42(12):1501-6.

118 Librizzi L; Janigro D; De BS; de CM. Blood-brain barrier preservation in the in vitro isolated guinea pig brain preparation. J Neurosci.Res. 2001 Oct 15;66(2):289-97.

119 McAllister MS; Krizanac-Bengez L; Macchia F; Naftalin RJ; Pedley KC; Mayberg MR, Janigro D. Mechanisms of glucose transport at the blood-brain barrier: an in vitro study. Brain Res. 2001 Jun 15;904(1):20-30.

120 Oh CW; Bump EA; Kim JS; Janigro D; Mayberg MR. Induction of a senescence-like phenotype in bovine aortic endothelial cells by ionizing radiation. Radiat.Res. 2001 Sep;156(3):232-40.

121 Cavaglia M; Dombrowski SM; Drazba J; Vasanji A; Bokesch PM; Janigro D. Regional variation in brain capillary density and vascular response to ischemia. Brain Res. 2001 Aug 10;910(1-2):81-93.

122 Strelow LI; Janigro D; Nelson JA. The blood-brain barrier and AIDS. Adv.Virus Res. 2001;56:355-88.

123 Grant GA; Abbott NJ; Janigro D. Understanding the Physiology of the Blood-Brain Barrier: In Vitro Models. News Physiol Sci. 1998 Dec;13:287-93.

124 Najm I; Ying Z; Janigro D. Mechanisms of epileptogenesis. Neurol.Clin 2001 May;19(2):237-50.

125 Janigro D; Stanness KA; Soderland C; Grant GA. Development of an in vitro blood-brain barrier. Curr.Protoc.Toxicol. 2001;Chapter 12:Unit12.

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126 Sinclair CJ; Krizanac-Bengez L; Stanness KA; Janigro D; Parkinson FE. Adenosine permeation of a dynamic in vitro blood-brain barrier inhibited by dipyridamole. Brain Res. 2001 Apr 13;898(1):122-5.

127 Emmi A; Wenzel HJ; Schwartzkroin PA; Taglialatela M; Castaldo P; Bianchi L, Janigro D.Do glia have heart? Expression and functional role for ether-a-go-go currents in hippocampal astrocytes. J Neurosci. 2000 May 15;20(10):3915-25.

128 Stanness KA; Neumaier JF; Sexton TJ; Grant GA; Emmi A; Maris DO, Janigro D.A new model of the blood--brain barrier: co-culture of neuronal, endothelial and glial cells under dynamic conditions. Neuroreport 1999 Dec 16;10(18):3725-31.

129 Nguyen TS; Winn HR; Janigro D. ATP-sensitive potassium channels may participate in the coupling of neuronal activity and cerebrovascular tone. Am J Physiol Heart Circ.Physiol 2000 Mar;278(3):H878-H885.

130 Janigro D. Blood-brain barrier, ion homeostatis and epilepsy: possible implications towards the understanding of ketogenic diet mechanisms. Epilepsy Res. 1999 Dec;37(3):223-32.

131 D'Ambrosio R; Maris DO; Grady MS; Winn HR; Janigro D. Impaired K(+) homeostasis and altered electrophysiological properties of post-traumatic hippocampal glia. J Neurosci. 1999 Sep 15;19(18):8152-62.

132 Janigro D; Leaman SM; Stanness KA. Dynamic modeling of the blood-brain barrier: a novel tool for studies of drug delivery to the brain. Pharm.Sci.Technolo.Today 1999 Jan;2(1):7-12.

133 Hochman DW; D'Ambrosio R; Janigro D; Schwartzkroin PA. Extracellular chloride and the maintenance of spontaneous epileptiform activity in rat hippocampal slices. J Neurophysiol. 1999 Jan;81(1):49-59.

134 D'Ambrosio R; Maris DO; Grady MS; Winn HR; Janigro D. Selective loss of hippocampal long-term potentiation, but not depression, following fluid percussion injury. Brain Res. 1998 Mar 9;786(1-2):64-79.

135 D'Ambrosio R; Wenzel J; Schwartzkroin PA; McKhann GM; Janigro D. Functional specialization and topographic segregation of hippocampal astrocytes. J Neurosci. 1998 Jun 15;18(12):4425-38.

136 Pekny M; Stanness KA; Eliasson C; Betsholtz C; Janigro D. Impaired induction of blood-brain barrier properties in aortic endothelial cells by astrocytes from GFAP-deficient mice. Glia 1998 Apr;22(4):390-400.

137 Stanness KA; Westrum LE; Fornaciari E; Mascagni P; Nelson JA; Stenglein SG, Janigro D. Morphological and functional characterization of an in vitro blood-brain barrier model. Brain Res. 1997 Oct 17;771(2):329-42.

138 Janigro D; Martenson ME; Baumann TK. Preferential inhibition of Ih in rat trigeminal ganglion neurons by an organic blocker. J Membr.Biol. 1997 Nov 15;160(2):101-9.

139 McKhann GM; D'Ambrosio R; Janigro D. Heterogeneity of astrocyte resting membrane potentials and intercellular coupling revealed by whole-cell and gramicidin-perforated patch recordings from cultured neocortical and hippocampal slice astrocytes. J Neurosci. 1997 Sep 15;17(18):6850-63.

140 Janigro D; Nguyen TS; Meno J; West GA; Winn HR. Endothelium-dependent regulation of cerebrovascular tone by extracellular and intracellular ATP. Am J Physiol 1997 Aug;273(2 Pt 2):H878-H885.

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141 Barth A; Newell DW; Nguyen LB; Winn HR; Wender R; Meno JR, Janigro D. Neurotoxicity in organotypic hippocampal slices mediated by adenosine analogues and nitric oxide. Brain Res. 1997 Jul 11;762(1-2):79-88.

142 Janigro D; Gasparini S; D'Ambrosio R; McKhann G; DiFrancesco D. Reduction of K+ uptake in glia prevents long-term depression maintenance and causes epileptiform activity. J Neurosci. 1997 Apr 15;17(8):2813-24.

143 Janigro D; Wender R; Ransom G; Tinklepaugh DL; Winn HR. Adenosine-induced release of nitric oxide from cortical astrocytes. Neuroreport 1996 Jul 8;7(10):1640-4.

144 Ransom CB; Sontheimer H; Janigro D. Astrocytic inwardly rectifying potassium currents are dependent on external sodium ions. J Neurophysiol. 1996 Jul;76(1):626-30.

145 Janigro D; Nguyen TS; Gordon EL; Winn HR. Physiological properties of ATP-activated cation channels in rat brain microvascular endothelial cells. Am J Physiol 1996 Apr;270(4 Pt 2):H1423-H1434.

146 Guatteo E; Stanness KA; Janigro D. Hyperpolarization-activated ion currents in cultured rat cortical and spinal cord astrocytes. Glia 1996 Mar;16(3):196-209.

147 Stanness KA; Guatteo E; Janigro D. A dynamic model of the blood-brain barrier "in vitro". Neurotoxicology 1996;17(2):481-96.

148 Maccaferri G; Janigro D; Lazzari A; DiFrancesco D. Cesium prevents maintenance of long-term depression in rat hippocampal CA1 neurons. Neuroreport 1994 Sep 8;5(14):1813-6.

149 Janigro D; West GA; Nguyen TS; Winn HR. Regulation of blood-brain barrier endothelial cells by nitric oxide. Circ.Res. 1994 Sep;75(3):528-38.

150 Janigro D; West GA; Gordon EL; Winn HR. ATP-sensitive K+ channels in rat aorta and brain microvascular endothelial cells. Am J Physiol 1993 Sep;265(3 Pt 1):C812-C821.

151 DiFrancesco D; Porciatti F; Janigro D; Maccaferri G; Mangoni M; Tritella T, Janigro D.Block of the cardiac pacemaker current (If) in the rabbit sino-atrial node and in canine Purkinje fibres by 9-amino-1,2,3,4-tetrahydroacridine. Pflugers Arch. 1991 Feb;417(6):611-5.

152 Janigro D; Maccaferri G; Meldolesi J. Calcium channels in undifferentiated PC12 rat pheochromocytoma cells. FEBS Lett. 1989 Sep 25;255(2):398-400.

153 Janigro D; Maccaferri G; Meldolesi J. Are undifferentiated PC12 rat pheochromocytoma cells electrically excitable? Biochem.Biophys.Res.Commun. 1989 Sep 15;163(2):810-4.

154 Janigro D; Schwartzkroin PA. Effects of GABA on CA3 pyramidal cell dendrites in rabbit hippocampal slices. Brain Res. 1988 Jun 21;453(1-2):265-74.

155 Janigro D; Schwartzkroin PA. Effects of GABA and baclofen on pyramidal cells in the developing rabbit hippocampus: an 'in vitro' study. Brain Res. 1988 Jun 1;469(1-2):171-84.

156 Janigro D; Schwartzkroin PA. Dissociation of the IPSP and response to GABA during spreading depression-like depolarizations in hippocampal slices. Brain Res. 1987 Feb 24;404(1-2):189-200.

157 Janigro D; Costa LG. Effects of trimethyltin on granule cells excitability in the in vitro rat dentate gyrus. Neurotoxicol.Teratol. 1987 Jan;9(1):33-8.

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158 Davis AK; Janigro D; Schwartzkroin PA. Effects of tissue preincubation and hypoxia on CA3 hippocampal neurons in the in vitro slice preparation. Brain Res. 1986 Apr 2;370(1):44-53.

159 Bianchi R; Janigro D; Milan F; Giudici G; Gorio A. In vivo treatment with GM1 prevents the rapid decay of ATPase activities and mitochondrial damage in hippocampal slices. Brain Res. 1986 Feb 5;364(2):400-4.

160 Kojima H; Gorio A; Janigro D; Jonsson G. GM1 ganglioside enhances regrowth of noradrenaline nerve terminals in rat cerebral cortex lesioned by the neurotoxin 6-hydroxydopamine. Neuroscience 1984 Dec;13(4):1011-22.

161 Jonsson G; Gorio A; Hallman H; Janigro D; Kojima H; Zanoni R. Effect of GM1 ganglioside on neonatally neurotoxin induced degeneration of serotonin neurons in the rat brain. Brain Res. 1984 Nov;318(2):171-80.

162 Gorio A; Janigro D; Zanoni R. Neuritogenesis and regeneration in the nervous system: an overview of the problem and on the promoting action of gangliosides. Adv.Exp.Med.Biol. 1984;174:465-73.

163 Jonsson G; Gorio A; Hallman H; Janigro D; Kojima H; Luthman J, Janigro D.Effects of GM1 ganglioside on developing and mature serotonin and noradrenaline neurons lesioned by selective neurotoxins. J Neurosci.Res. 1984;12(2-3):459-75.

164 Gorio A; Ferrari G; Fusco M; Janigro D; Zanoni R; Jonsson G. Gangliosides and their effects on rearranging peripheral and central neural pathways. Cent.Nerv.Syst.Trauma 1984;1(1):29-37.

165 Janigro D; Di GF; Vyskocil F; Gorio A. Gangliosides' dual mode of action: a working hypothesis. J Neurosci.Res. 1984;12(2-3):499-509.

Books

Humana Press 2006: Cell Cycle in the Central nervous system, DAMIR JANIGRO EDITOR Humana Press 2009: Human Brain Development, DAMIR JANIGRO EDITOR

Selected Book Chapters 1. JONSSON, G., GORIO, A., HALLAMAN, H., JANIGRO, D., KOJIMA, H. and ZANONI, R.: Effects of GM1 gangliosides on the postnatal development of monoamine neurons in rat CNS after neurotoxin induced damage in neonatal stage. In: Regulation of Transmitter Function. Edited by E.S. Vizi and K. Magyar, pp. 559-562 (1984) 2. GORIO, A., JANIGRO, D., DI GREGORIO, F. and JONSSON, G.: Gangliosides as reagents that promote synaptic plasticity and recovery of functions. In: Synaptic Plasticity. Edited by C.W. Cotman. The Guilford Press: New York, 507-540 (1985) 3. GORIO, A., JANIGRO, D. and ZANONI, R.: Neuritogenesis and regeneration in the nervous system: an overview of the problem and on the promoting action of gangliosides. In: Ganglioside Structure, Function and

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Biomedical Potential. Edited by R.W. Ledeen, R.K. Yu, N.M. Rapport and K. Suzuki. Plenum Press Publishing Corporation: New York, pp. 465-473 (1984) 4. GORIO, A., FERRARI, G., FUSCO, M., JANIGRO, D., ZANONI, R. and JONSSON, G.: Gangliosides and their effects on rearranging peripheral and central neural pathways. In: Central Nervous System Trauma. Mary Ann Liebert Inc. Publishers: New York, vol. 1, pp. 29- 37 (1984) 5. GORIO, A., DI GREGORIO, F., JANIGRO, D., MARINI, P., SILIPRANDI, R., ZANINI, R. and JONSSON, G.: Regenerative capacity in the nervous system, enhancement by exogenous gangliosides. In: Neuromodulation and Brain Function, Vol. 48, Advances in the Biosciences. Edited by G. Biggio, P.F. Spano, G. Toffano and R.L. Gessa. Pergamon Press: New York, pp. 363-372 (1984) 6. GORIO, A., DI GREGORIO, F. and JANIGRO, D.: Gangliosides as potential repair agents after neuronal injury. In: Clinical Neurophysiology in Peripheral Neuropathies. Edited by P.J. Delwaide and A. Gorio. Elsevier Science Publishers B.V., pp. 23-40 (1985). 7. GORIO, A., CARMIGNOTO, G., FUSCO, M., DI GREGORIO, F., JANIGRO, D., VYSKOCIL, F. and JONSSON, G.: Gangliosides and neuronal plasticity. In: Regulation of Transmitter Function. Edited by E.S. Vizi and K. Magyar, pp. 395-397 (1984) 8. GORIO, A., JONSSON, G., COMELLI, C., CANELLA, R., JANIGRO, D., ZANONI, R. and CARMIGNOTO, G.: GM1 modulates neuronal plasticity in developing animals. Int. J. Develop. Neurosci. 3 (4), 425 (1985) 9. JANIGRO, D. and JOSEPH, M. (1997) Cost Effective Alternatives to High Priced Software for the small Research Laboratory, Scientific Computing & Automation, 10(7), 13-17. 10. JANIGRO, D. and WINN, HR: Chloroadenosine induces neurotoxicity in hippocampal slices. in “Role of adenosine in the central nervous system”, Y. Okada and B.B. Fredholm, Eds., 1997 (Elsevier) 11. JANIGRO, D., STANNESS, K., NGUYEN, TS, TINKLEPAUGH, D. and H. R. WINN: Physiological Properties and Function of ATP-Activated Cation Channels ”, Kluwe Academic Press, L. Belardinelli and A. Pelleg Eds. (1994) 12. GRANT, G.A., ABBOTT, N.J., JANIGRO, D. (1998) Understanding the Physiology of the Blood-Brain Barrier: in vitro Models, News in Physiological Sciences, invited review, Vol. 14, Nov. 1998 13. MCKHANN, GM and JANIGRO, D. “Electrophysiology of the Nervous System” in: Youman’s Neurological Surgery, 5th Ed., Saunders, 1.1.8, 2003 and 2010 14. GRANT, GA and JANIGRO, D. “The blood-brain barrier”: in Youman’s Neurological Surgery, 5th Ed.,

Saunders, 1.1.5, 2003 and 2010 15. NAJM I., YING, Z. and JANIGRO, D. Mechanisms of epileptogenesis.

Neurol Clin. 2001 May;19(2):237-50 16. NAJM, I., JANIGRO, D., BABB, T. (2001) Mechanisms in epileptogenesis and experimental

seizure models, in: The treatment of epilepsies, Principles and practice 3rd ed. Philadelphia: Lippincott Williams & Wilkins, 2001. 3,33-44.

17. JANIGRO, D. Microenvironment and Neuronal Excitability in: The Neuronal microenvironment, W. Walz

Ed. Humana Press, 2001 p. 3-25.

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18. MARRONI, M, KIGHT, K., HOSSEIN, M., DESAY, SY and JANIGRO, D. Dynamic in vitro model of the

blood-brain barrier: Gene profiling using cDNA analysis. Methods in Molecular Medicine vol. 89,(419-434) BBB Research Protocols, S. Nag, Ed., Humana Press, 2003.

19. Cucullo L., Marchi N., Fazio V., Nguyen M., and JANIGRO D. “Blood-brain barrier, blood flow and

Neoplasms: the role of astrocytes” in Epilepsy: Mechanisms, Models, and Translational Perspectives. Rho, Jong Editor

20. Cerebrovascular diseases, seizures and epilepsy. “Encyclopedia of Basic Epilepsy”, Edited by Dr. Schwartzkroin (in press, 2010)

21. JANIGRO D. Drug movement across the blood-brain barrier – implications for pharmacoresistance. “Encyclopedia of Basic Epilepsy”, Edited by Dr. Schwartzkroin (2010)

22. JANIGRO D. Blood-brain barrier, blood flow and epilepsy: the role of astrocytes (2010), Edited by Dr. Wong

23. JANIGRO D. Other factors modulating seizure susceptibility. “Atlas of Epilepsies” (2010). Edited by Drs. Moshe’ and Galanopoulou.