Crude Oil Phytoremediation Investigation With Different ...
Transcript of Crude Oil Phytoremediation Investigation With Different ...
Crude Oil Phytoremediation Investigation With Different Poplar And Willow Clones
Andrej Pilipovic(1), Sasa Orlovic(1), Natasa Nikolic(2), Borivoj Krstic(2), Karolina Nemes(2)
1 University of Novi Sad, Institute of Lowland Forestry and Environment, Serbia
2 University of Novi Sad, Faculty of Sciences – Institute of Biology and Ecology, Serbia
Novi Sad, Serbia April-May 1999
•Destroyed - 74 000 t of crude oil and derivates
•90% burned
•surface runoff to Danube - 500t
•Rest – soil contamination 95 000 m2
(51 000 m2 with crude oil)
•Collected contaminated soils transferred to special concrete pools
Rhizodegradation
Biotransformation processes Enzymatic activity of microorganisms:
oxidation,reduction,hidrolysis,conjugation etc.
Interaction of plants and microorganisms with aim of mutual benefits
Exu
date
s
Detoxification
Goal of research - Questions
possible to phytoremediatie?
Can P&W grow on such soil?
How P&W grow?
Rhizodegradation?
Mixed or as it is?
Material and methods
Cuttings from three poplar clones:Populus deltoides cl. PD2; Populus X euramericana cl. EA8 and Populus nigra X maximowiczii X Populus nigra var. „Italica“ cl. NM8 and
Three willow clones:Salix alba cl. SA/H/1; Salix alba cl. SA/I/1 i Salix alba cl. SA/K/1
in greenhouse pot experiment
Material and methods
treatmentTPH mg/kg
mineral oilsmg/kg
0% 20.83 5.12
5% 76.88 54.87
25% 812.96 571.8
50% 6046.6 3769
75% 9311.7 5370
100% 10841.6 6174
Material and methods
Investigated parametersiBiomass productionPlant heightNitrate reductase activity (NRA)
Microorganisms abundance
Plant height (cm)
0
10
20
30
40
50
60
70
80
0% 5% 25% 50% 75% 100%
tretman
h (c
m)
EA8 NM3 PD2 SA/I/1 SA/H/1 SA/K/1
Biomass production (gFW )
02468
1012141618
0% 5% 25% 50% 75% 100%
tretman
M(g
DW
)
EA8 NM3 PD2 SA/I/1 SA/H/1 SA/K/1
M (g
sv.m
at.)
25% 5% 0%
100% 75% 50%
Clone
Treatment
0% 5% 25% 50% 75% 100%
EA8 0,574cde 0,368fgh 0,782a 0,389fg 0,670abcd 0,672abcd
NM3 0,259hi 0,217i 0,438f 0,639bcd 0,465ef 0,338fghi
PD2 0,407fg 0,303ghi 0,743ab 0,412fg 0,699abc 0,568de
SA/I/1 0,0426cde 0,0313e 0,0387cde 0,0460bcd 0,0360de 0,0320e
SA/H/1 0,0510abc 0,0393cde 0,0397cde 0,0377de 0,0337de 0,0327e
SA/K/1 0,0627a 0,0393cde 0,0553ab 0,0400cde 0,0440bcde 0,0370de
LSD(0,05)=0,1151 for poplars; 0,0115 for willows
NRA (µmol NO2 g-1fw h-1)
Chlorophyll fluorescence Fv/Fm
Clone
Treatment
0% 5% 25% 50% 75% 100%
EA8 0,724abc 0,700abcd 0,647bcdef 0,707abcde 0,712abcde 0,733abcd
NM3 0,744abc 0,715abcde 0,731abcde 0,731abcde 0,697abcdef 0,724abcd
PD2 0,625def 0,607ef 0,669abcdef 0,654abcdef 0,375g 0,756ab
SA/I/1 0,721abcd 0,750ab 0,750ab 0,756ab 0,725abcd 0,694abcdef
SA/H/1 0,755ab 0,762a 0,729abcd 0,745abc 0,684abcdef 0,639cdef
SA/K/1 0,744abc 0,715abcde 0,672abcdef 0,627def 0.625def 0,588f
LSD(0,05)=0,0921
Microorganisms abundanceoil-oxydizing bacteria
0.00E+002.00E+064.00E+066.00E+068.00E+061.00E+071.20E+071.40E+07
0% 5% 25% 50% 75% 100%tretman
broj
nost
EA8 NM3 PD2 SA/I/1 SA/H/1 SA/K/1 kontrola
Organotrophic microorganisms
0.00E+00
2.00E+06
4.00E+06
6.00E+06
8.00E+06
1.00E+07
0% 5% 25% 50% 75% 100%
tretman
broj
nost
Facultative organotrophic microorganisms
0.00E+00
2.00E+06
4.00E+06
6.00E+06
8.00E+06
1.00E+07
1.20E+07
0% 5% 25% 50% 75% 100%tretman
broj
nost
Enzymatic activity
0
2000000
4000000
6000000
8000000
10000000
12000000
14000000
EA
8N
M3
PD
2S
A/I/
1S
A/H
/1S
A/K
/1 ØE
A8
NM
3P
D2
SA
/I/1
SA
/H/1
SA
/K/1 Ø
EA
8N
M3
PD
2S
A/I/
1S
A/H
/1S
A/K
/1 ØE
A8
NM
3P
D2
SA
/I/1
SA
/H/1
SA
/K/1 Ø
EA
8N
M3
PD
2S
A/I/
1S
A/H
/1S
A/K
/1 ØE
A8
NM
3P
D2
SA
/I/1
SA
/H/1
SA
/K/1 Ø
CFU/
cm-3
0
500
1000
1500
2000
2500
3000
3500
4000
4500
PAI m
ol p
Np/s
/dm
-3
abundancePAI
0% 5% 25% 50% 75% 100%
Conclusions - AnswersOil affected biomass production of P&WEnzymatic activity in plants and chlorophyll fluorescence measurement showed relative stability in unfavorable growing conditions.Growth results showed differentiation between P&W, although trends were in toxicity effect were similarResults showed possibility for plants to grow on such soilsP & W increased abundance of microorganisms, especially oil-oxydizingmicrobes
Thank You for Attention!!!