Corporate Statement of Capabilities Corporate Office/Mailing ...
Corporate Capabilities 2018 - PharmTechfiles.pharmtech.com/alfresco_images/pharma/2019/01/... ·...
Transcript of Corporate Capabilities 2018 - PharmTechfiles.pharmtech.com/alfresco_images/pharma/2019/01/... ·...
Application Notes ♦ See page 55AAAAAAAppppppppllllllliiiiiiiccccaaaattttttiiiiiiioooonnnn NNNNNNNooootttttteeeessss ♦♦♦♦♦♦♦ SSSSSSSeeeeeeee ppppaaaaggggeeee 555555555555
Corporate Capabilities
2018A Guide to Leading Separation Science Suppliers
LC
GC
NO
RT
H A
ME
RIC
A C
orp
ora
te C
ap
ab
ilities —
20
18
© 2014 Hamilton Company. All rights reserved.
Your sample depends on it
More than 50 years of precision liquid handling experience. Proven accuracy. Continuous research and development. Choose an autosampler syringe from Hamilton Company and be confident in your results.
Hamilton offers a line of syringes designed to work with a wide range of the most popular autosamplers from Agilent, CTC PAL, Spark Holland and more. Each syringe is expertly handcrafted to maximize sample integrity, process efficiency and new long-life syringe technology.
For more information, visit
www.ham-info.com/0804-1
or call toll free 1-888-525-2123.
A touch of intelligenceMilli-Q®�,4������SXULɟ�FDWLRQ�V\VWHP
• New ultrapure water system with intuitive touch screen
• Innovative Q-POD®�GLVSHQVHU�GHVLJQHG�WR�PD[LPL]H�Hɡ��FLHQF\
• Environmentally friendly mercury-free UV oxidation lamps
The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
HPGPLOOLSRUH�FRP�PLOOLT�LTV\VWHP
MilliporeSigma, Milli-Q, and the vibrant M are trademarks and Q-POD is a registered trademark of Merck KGaA, Darmstadt, Germany. Copyright © 2017 EMD Millipore Corporation. All Rights Reserved.
2017 - 03495
4 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 WWW.CHROMATOGRAPHYONLINE.COM
UBM Americas (www.ubmlifesciences.com) is a leading worldwide media com-pany providing integrated marketing solutions for the Fashion, Life Sciences and Powersports industries. UBM Americas serves business professionals and consum-ers in these industries with its portfolio of 91 events, 67 publications and directories, 150 electronic publications and Web sites, as well as educational and direct market-ing products and services. Market leading brands and a commitment to delivering innovative, quality products and services enables UBM Americas to “Connect Our Customers With Theirs.” UBM Americas has approximately 1000 employees and currently operates from multiple offices in North America and Europe.
© 2017 UBM. All rights reserved. No part of this publication may be reproduced or
transmitted in any form or by any means, electronic or mechanical including by pho-
tocopy, recording, or information storage and retrieval without permission in writ-
ing from the publisher. Authorization to photocopy items for internal/educational or
personal use, or the internal/educational or personal use of specifi c clients is grant-
ed by UBM for libraries and other users registered with the Copyright Clearance
Center, 222 Rosewood Dr. Danvers, MA 01923, 978-750-8400 fax 978-646-8700 or
visit http://www.copyright.com online. For uses beyond those listed above, please
direct your written request to Permission Dept. fax 440-756-5255 or email: Maureen.
UBM Americas provides certain customer contact data (such as customer’s name,
addresses, phone numbers, and e-mail addresses) to third parties who wish to pro-
mote relevant products, services, and other opportunities that may be of interest to
you. If you do not want UBM Americas to make your contact information available
to third parties for marketing purposes, simply call toll-free 866-529-2922 between
the hours of 7:30 a.m. and 5 p.m. CST and a customer service representative will as-
sist you in removing your name from UBM Americas lists. Outside the U.S., please
phone 218-740-6477.
LCGC North America does not verify any claims or other information appearing in
any of the advertisements contained in the publication, and cannot take responsibil-
ity for any losses or other damages incurred by readers in reliance of such content.
To subscribe, call toll-free 888-527-7008. Outside the U.S. call 218-740-6477.
MANUSCRIPTS: For manuscript preparation guidelines, see chromatographyon-
line.com/lcgc-author-guidelines, or call The Editor, (732) 596-0276. LCGC welcomes
unsolicited articles, manuscripts, photographs, illustrations, and other materials
but cannot be held responsible for their safekeeping or return. Every precaution is
taken to ensure accuracy, but LCGC cannot accept responsibility for the accuracy
of information supplied herein or for any opinion expressed.
SUBSCRIPTIONS: For subscription and circulation information: LCGC, P.O. Box 6168,
Duluth, MN 55806-6168, or call (888) 527-7008 (7:00 a.m.–6:00 p.m. central time).
International customers should call +1-218-740-6477. Delivery of LCGC outside the
United States is 14 days after printing. For single and back issues, call (800) 598-
6008 or (218) 740-6480. (LCGC Europe and LCGC Asia Pacific are available free of
charge to users and specifiers of chromatographic equipment in Western Europe
and Asia and Australia, respectively.)
CHANGE OF ADDRESS: Send change of address to LCGC, P.O. Box 6168, Duluth,
MN 55806-6168; alternately, send change via e-mail to [email protected] or
go to the following URLs:
• Print: http://ubmsubs.ubm.com/?pubid=LCGC
• Digital: http://ubmsubs.ubm.com/?pubid=LCGC&V=DIGI
Allow four to six weeks for change. PUBLICATIONS MAIL AGREEMENT
No. 40612608. Return all undeliverable Canadian addresses
to: IMEX Global Solutions, P.O. Box 25542, London, ON, N6C
6B2, CANADA. Canadian GST number: R-124213133RT001.
C.A.S.T. DATA AND LIST INFORMATION: Contact Melissa Stillwell,
tel. (218) 740-6831, e-mail [email protected].
REPRINTS: Reprints of all articles in this issue and past issues of this publication
are available (500 minimum). Call 877-652-5295 ext. 121 or e-mail
[email protected]. Outside US, UK, direct dial: 281-419-5725 ext. 121
INTERNATIONAL LICENSING: Contact Maureen Cannon, tel.
(440) 891-2742, fax (440) 891-2650, or e-mail [email protected].
485F US Highway One South, Suite 210 Iselin, NJ 08830
(732) 596-0276 • Fax: (732) 647-1235
Michael J. TessaloneVice President/Group Publisher [email protected]
Edward FantuzziPublisher [email protected]
Stephanie ShafferSales Manager [email protected]
Brianne MolnarSales Manager [email protected]
Michael KushnerSenior Director, Digital [email protected]
Kristen MooreWebcast Operations Manager [email protected]
Vania OliveiraProject Manager [email protected]
Sabina AdvaniDigital Production Manager [email protected]
Kaylynn Chiarello-EbnerManaging Editor, Special Projects [email protected]
Anne Lavigne Marketing Manager [email protected]
Melissa StillwellC.A.S.T. Data and List Information [email protected]
Laura BushEditorial Director [email protected]
Megan L’HeureuxManaging Editor Meg.L’[email protected]
Stephen A. BrownGroup Technical Editor [email protected]
Cindy DelonasAssociate Editor [email protected]
Dan WardArt Director [email protected]
Rajesh ThangappanGraphic Designer [email protected]
Wright’s MediaReprints [email protected]
Maureen CannonPermissions [email protected]
Jesse SingerProduction Manager [email protected]
Wendy BongAudience Development Manager [email protected]
Matt BlakeAudience Development Assistant Manager [email protected]
Corporate CapabilitiesCorporate Capabilities2 0 1 8
Corporate Profiles
8 ACCTA, Inc.
9 Antec Scientific
10 Advanced Chemistry
Development, Inc. (ACD/Labs)
12 Biotage, LLC
13 CDS Analytical
14 CEM Corporation
15 CTC Analytics
16 Chem Service, Inc.
18 FMS, Inc.
19 Fritsch Milling & Sizing, Inc.
20 Hamilton Company
21 Imtakt USA
22 LECO Corporation
24 MACHEREY-NAGEL GMBH &
CO. KG
25 Markes International
26 MicroSolv Technology
Corporation
28 MilliporeSigma
29 Mott Corporation
30 Neta Scientific Inc.
31 Parker Hannifin
32 PerkinElmer, Inc.
33 PHOTONIS USA
34 PSS Polymer Standards
Service Inc.
35 Molex
36 Postnova Analytics Inc.
37 Proton OnSite
38 Quantum Analytics
39 Restek Corporation
40 Sciencix
41 Shodex/Showa Denko
America, Inc.
42 Shimadzu Scientific
Instruments
44 SilcoTek® Corporation
45 Sorbent Technologies, Inc.
46 Tosoh Bioscience LLC
47 UCT, Inc.
48 Valco Instruments Co., Inc.
(VICI)
50 Wyatt Technology Corp.
52 YMC America, Inc.
54 ZirChrom Separations, Inc.
Application Notes
55 Medical/Biological
58 Food & Beverage
62 Environmental
63 Pharma/Drug Discovery
64 Polymer
65 General
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 5www.chromatographyonline.com
SPONSORED BY
Current Trends in Chiral SeparationsA Q&A
A discussion of the trends, development, and use of
chiral stationary phases and separation modes
Chiral compounds such as pharmaceuticals, pesticides, and raw materials are
challenging to work with because enantiomeric species can provide varying physio-
chemical properties. Chromatography products and systems are available for use in a
range of separation modes to assist in the accurate separation and analysis of these distinct
compound forms. LCGC recently sat down with Marc Jacobs, PhD, to discuss trends in the
development of chiral columns and his advice on choosing columns and separation modes
for chiral screening.
LCGC: How did you initially become interested in the field of chirality?Jacob: Early in my academic career in France, I was a student of Professor Pierre Duhamel,
a pioneer of asymmetric synthesis. I was introduced to the concept of chirality in one of his
classes. My PhD thesis was on the topic of asymmetric synthesis of amino acids, which are
important chiral natural products. In France, chirality has historical roots from long ago when
Louis Pasteur separated tartaric acid using a microscope. I’ve been involved in the field of
chiral analysis for the last 20+ years.
LCGC: Why is there so much emphasis on chiral analysis and the detection of enantiomers in pharmaceutical products?Jacob: Chiral purity is particularly important in pharmaceuticals. Because pharmaceutical prod-
ucts are developed for use in humans, you must ensure that the purity of the pharmaceutical
ingredient is well understood and characterized. Since the 1990s, the FDA has required the
analysis of the enantiomers for a chiral drug in development. Many drugs before the 1990s
were developed as racemates. Pharmaceutical analysts found that sometimes an enantiomer,
also called a distomer, can have no activity, and in some cases, can have toxic activity. The
good enantiomer is referred to as the “eutomer.”
The case of thalidomide raised the importance of chiral analysis. The drug was used in the
1950s as a sedative and to alleviate morning sickness in pregnant women. One of the enantio-
mers of the drug, however, possessed some adverse properties, and many children were born
with severe deformities. After the discovery of these effects, the FDA required the analysis of
chiral drugs for all of the existing enantiomers to determine whether or not toxic properties exist.
LCGC: What recent improvements have been made in the kinds of chiral columns in use today for analytical and preparative separations?Jacob: Most of the major developments in chiral chromatography took place in the 1980s
with the introduction of the polysaccharide-based chiral stationary phase, which is the most
useful phase for analytical and preparative separations. In the 1980s, just two different types of
phases were used, which were based on a cellulose backbone or an amylose backbone. Now,
we have a dozen or more stationary phases. Current developments are focused on generating
different chiral selectors on those polysaccharide-based stationary phases.
Marc Jacob, PhD
Global Product Manager for Chiral and Preparative
Chromatography Phenomenex
CURRENT TRENDS IN CHIRAL SEPARATIONS
About 10 years ago, the immobilized version of polysaccharide-
based stationary phases was introduced. These phases were
coated on the silica material, which created issues with the
solubilization of the chiral selector during a chromatographic
analysis. The current immobilized version is robust and
opened up opportunities for chiral separations because it
allows for the use of more types of organic solvents. With
a chiral separation, you cannot predict which phase will
separate the two enantiomers. Typically, quite a few columns
and mobile phases must be screened to obtain good results.
LCGC: Do you still see a need for both coated and immobilized polysaccharide chiral columns?Jacob: My visits to customers indicate that coated phases are
not disappearing and are useful in terms of improving success.
Because you cannot predict the separation, you need a high
success rate with the column or columns being used. Analysts
continue to use both coated and immobilized columns in their
screens, especially in SFC, as well as in normal-phase and
reversed-phase chromatography. Immobilized phases are of
specific interest because they give you access to a new chiral
selector and enhance your chance of finding a successful chiral
separation.
LCGC: What separation modes are most widely used for chiral separations?Jacob: Historically, normal-phase mode has been used, which
is a mixture of an alkane and alcohol, for example, hexane and
ethanol or hexane and IPA. The polar organic mode (POM),
which is a mixture of two organic solvents, has also been
used. POM uses methanol and acetonitrile or ethanol and
acetonitrile in the mobile phase, which are compatible with
coated or immobilized phases. The variety of solvents that
could be used was limited originally because primarily coated
stationary phases were used.
Approximately 90% of chiral separations are carried out on
polysaccharide-based phases. In the last 20 years, super-
critical fluid chromatography (SFC) has become prominent
because of its speed. With SFC, there is less viscosity from
the solvent in the mobile phase, so separations can be run
at higher flow rates—almost four times faster—which results
in faster screening. SFC mobile phases contain at least
60% carbon dioxide (CO2), so there is less mobile phase to
evaporate, which is important in preparative chromatography.
Analytical-scale separation use much less solvent overall,
which makes it a “green” technology. Analytical separa-
tions are powerful using polysaccharide-based columns in
reversed-phased mode. Analysts thought these phases would
not be stable, but reversed-phase separations using water/
acetonitrile or water/methanol demonstrate good results, and
their use is growing.
We did some work on FMOC amino acids, which are
an important chiral raw material as a building block for the
synthesis of peptides. Reversed-phase chromatography was
able to separate all of the natural protected amino acids. With
immobilized chiral stationary phases, a variety of solvents
can be considered. In the polar organic mode, tetrahydrofuran
(THF) can be mixed with ethyl acetate or MTBE with methanol—
many combinations are possible. This type of approach is not
common, however, and it is expensive to use these organic
solvents. Consequently, historically, normal phase has been
used most commonly for chiral analysis, with SFC being used
for preparative separations and in pharmaceutical analysis,
and reversed-phase for analytical methods.
LCGC: What recommendations do you have for sta-tionary phases and modes to start with when under-taking chiral LC screening projects?Jacob: I will address small molecules because we’re dis-
cussing chiral separations of pharmaceuticals, which gener-
ally have only one chiral center. Three to six chiral stationary
phases will yield about a 90% success rate in screenings.
I recommend Lux Amylose-1 and Cellulose-1, which
cover the polysaccharide backbones. These phases were
developed in the 1980s by Yoshio Okamoto and commercial-
ized. Another option is a chiral selector with an ester, called
Cellulose-3, that gives you another type of interaction with your
molecule. I would also include a chlorinated phase, Cellulose-4
or Cellulose-2, which were developed by Professor Bezhan
Chankvetadze, a collaborator of Phenomenex. I suggest a
couple of immobilized phases because these chiral selec-
tors are unique and not available as coated phases, like the
i-Cellulose-5. These six phases should give you almost 99%
success, but just Amylose-1, Cellulose-1, and Cellulose-3 will
give you about 75% success.
Regarding mode, I suggest SFC, if possible, for speed, as
well as polar-organic, reversed-phase, and normal-phase
modes depending on your equipment. In sum, three to six
phases and a couple of separation modes.
LCGC: Have you seen an increase in chiral separations in any industries other than pharma?Jacob: We see some chiral analysis of basic chemical raw
materials such as FMOC amino acids, which are a critical raw
material made all over the world. Chiral analysis is also used
in the agrochemical industry for chiral pesticides, herbicides,
and fungicides. Because these products are used in food,
it’s critical to determine whether there are adverse effects
stemming from their chemistry. Some chemicals used in the
electronics industry are chiral and require analysis and clas-
sification. Most of the pharmaceutical drugs that are chiral
are now being developed as pure enantiomer and require
a battery of tests. But it remains to be seen whether the
development of small molecule drugs will increase or stay
stable in the future as other types of drug are developed in
the pharmaceutical industry.
8 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
ACCTA, Inc.
3534 Jessie Ct.
St. Paul, MN 55125
T E L E P H O N E
(651) 731-3670
F A X
(651) 730-0965
E - M A I L
W E B S I T E
www.accta.com
N U M B E R O F E M P L O Y E E S
2
Y E A R F O U N D E D
1993
ACCTA, Inc. meet the unique goals of our clients and
give them a competitive edge. Customers
tell us that they can learn as much in four
days, working with us, as they would in four
months of unproductive trial-and-error effort
on their own. Our program includes:
⦁ Laboratory consulting: Our experience
in laboratories over three decades can
solve your HPLC method development,
method optimization, and laboratory or
method problems. ACCTA’s experience
will help you become a more strategic
laboratory operation. You will save at least
one month of time for each day we spend
working together. The solutions that come
from our consultative efforts are designed
to deliver significant and lasting benefits
to your lab, even after we leave.
⦁ Staff Training: “Good training is your
best lab instrument.” ACCTA’s experi-
enced laboratory consultants will help
your staff operate a chromatography in-
strument and understand the results. We
can make that learning easier and faster
with basic or advanced level hardware
training, covering HPLC, UHPLC, and GC.
We are ready to help you implement
customized ChemStation training from
experts who have used the software in
many different applications. Discover
how our flexible approach and innovative
thinking can benefit your laboratory op-
erations and increase your profitability.
⦁ Lab Life-Line: We know every lab is dif-
ferent and that’s why we’ve customized
each of our services to provide fact-based
analysis and strategies exclusively for your
individual needs. Our extensive experi-
ence and ability to get the job done effi-
ciently and cost-effectively are why we are
consistently selected to deliver obtainable
and sustainable improvements including:
reducing solvent costs, improving your
laboratory’s efficiency, data processing,
reporting times, staff mentoring, workflow
optimization, and more. Our goal is to
always leave our clients in a better place
than where we found them.
Column-Screening Facility The ACCTA laboratory provides HPLC
method development feasibility information
and specialized chemical analysis capabili-
ties. This rapid screening approach can help
get you started in the right direction, saving
you time and money.
Company Description ACCTA provides elite-level consulting and training services
to analytical laboratories. We specialize in making your
(laboratory) life easier!
⦁ Is your lab fully utilizing its staff and equipment?
⦁ Do you need new or better procedures and processes?
⦁ Are you looking for a range of technical support options?
Since 1993 we have been developing analytical solutions for our
customers’ analytical problems. These solutions produce stress-
relief and significant return on investment (ROI) for laboratories.
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ UHPLC
⦁ GC
⦁ GPC–SEC
Markets Served ⦁ Pharmaceutical
⦁ Food
⦁ Consumer products
⦁ Nutritional supplements
⦁ Chemicals and chemical additives
⦁ Petroleum
⦁ Independent laboratories
⦁ Government
⦁ Academia
⦁ Legal
⦁ And More!
Major Products/Services ACCTA associates have worked as consultants in the laboratory
for decades. No project is too small or too large—our team of
leading experts has the depth of knowledge to scale services to
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 9www.chromatographyonline.com
Antec Scientific
One Boston Place,
201 Washington Street,
Boston, MA 02108
T E L E P H O N E
(888) 572-0012
E - M A I L
W E B S I T E
www.antecscientific.com
Y E A R F O U N D E D
1990, since 2010 in USA
Antec Scientific Electrochemical Reactors
for MS and Synthesis
With the introduction of the ROXY™ EC
system, REDOX reactions can be monitored
directly by Mass Spectrometry (MS).
The on-line coupling of Electrochemical
Reactors with MS (EC/MS) has found
many application areas from proteomics,
lipidomics, drug metabolism, drug stability
testing, to environmental degradation.
Another emerging application of Antec’s
flow and batch reactors is in Flow Chemistry
for rapid synthesis of drugs, metabolites,
degradants (reference materials).
SenCell™ for Detection
Proprietary Adjustable Spacer Technology
(AST) for highest sensitivity (US Patent
9,310,330)
μ-PrepCell 2.0™ for Reactions
Redox reactions upfront MS: disulfide
bond reduction in proteins, mimicking
drug metabolism, drug stability testing/
degradation
SynthesisCell™ for Synthesis
Batch reactor for rapid synthesis of mg
quantities of products
Company Description For over 27 years Antec Scientific has been the world
leading supplier of analytical instrumentation based on
Electrochemistry (EC). Antec’s line of instruments include
Electrochemical Detectors (ECD), Analyzers built on HPLC-
ECD and Electrochemical Reactors for use with MS (EC/MS)
and Synthesis.
Electrochemical Detectors for HPLC
The DECADE™ line of ECDs (Elite™ and Lite™) have become the
benchmark in detection and can be used with any third party
(U)HPLC system. Different flow cells are available to cover a
broad range of applications from the analysis of antibiotics to
sugars or pharmaceuticals.
Dedicated Analyzers
The ALEXYS™ Analyzers based on HPLC with ECD are turnkey
instruments built for highest performance and to ensure ease-
in-use. Guaranteed applications have been developed with
the ALEXYS Neurotransmitter Analyzer for sensitive analysis in
microdialysates and brain tissue. Other application areas are
in clinical/diagnostics, food/beverage, environmental, and
drugs/pharmaceuticals analysis.
10 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Advanced Chemistry
Development, Inc.
(ACD/Labs)
8 King Street East, Suite 107
Toronto, Ontario, Canada
M5C 1B5
T E L E P H O N E
+1 4163683435
E - M A I L
W E B S I T E
acdlabs.com
Y E A R F O U N D E D
1994
Major Products/Services ⦁ ACD/AutoChrom—comprehensive
method development and optimization
software that supports a Quality by
Design (QbD) approach based on
chromatographic principles. From
strategy creation and investigation
of maximum chromatographic space
to execution of screening runs and
identification of the optimal method.
Optional instrument control for Agilent
ChemStation and Waters Empower.
⦁ ACD/LC Simulator—method simulation
software for multivariate parameter
optimization. Optimize gradient, solvent
ratio and concentration, temperature,
and pH. Also provides retention
prediction and automatic peak
matching between experimental runs.
⦁ Luminata—software for the efficient
and comprehensive management
of impurity data. Establish effective
process and analytical impurity
control strategies based on live
chromatographic and spectral data and
route knowledge, in a purpose-built
environment.
Advanced Chemistry Development, Inc. (ACD/Labs)
Company Description Advanced Chemistry Development, Inc. (ACD/Labs), is an
informatics company that develops and commercializes
solutions in support of R&D. Our software platforms unify
analytical and chemical information in a collaborative
environment; and provide tools that enable scientists to
extract, capture, and apply knowledge from analytical
experiments and predicted molecular property data. We
integrate with existing informatics systems and undertake
custom projects including enterprise-level automation.
We offer more than two decades of success helping
organizations accelerate R&D and leverage corporate
intelligence. ACD/Labs provides worldwide sales and support.
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ UHPLC
⦁ LC–MS
⦁ GC–MS
⦁ LC–MS/MS
Markets Served ACD/Labs tools support R&D in various industries including
pharma and biotech, chemicals, consumer goods, agrochemi-
cals, petrochemicals, academic, and government institutions.
www.acdlabs.com/autochrom
Software to streamline method
development using QbD principles
Direct Instrument Control
Agilent Chemstation & Waters Empower
Automated Peak Tracking
Follow all components between runs
Robust Methods through Simulation and Experimentation
Optimize multiple parameters simultaneously
Complete Data Management
Seamlessly store, retrieve, and organize methods and results
Comprehensive Reporting
Communicate easily
CCoCCCCoMa
Robust Methods through
veengg
ilyy
Better Methods, Faster
ACD/AutoChrom
12 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Biotage, LLC
10430 Harris Oaks Blvd. Ste C,
Charlotte, NC 28269
T E L E P H O N E
(704) 654-4900
F A X
(704) 654-4917
E - M A I L
W E B S I T E
www.biotage.com
N U M B E R O F E M P L O Y E E S
USA 75
Elsewhere 250
Y E A R F O U N D E D
1989
Biotage, LLC
Major Products/Services Sample Prep, Flash Chromatography,
Automation, Evaporation, Microwave
Synthesis, Peptide Synthesis
Facility Biotage USA, Biotage Sweden, Biotage UK,
Biotage Korea, Biotage Japan, and Biotage
China
Company Description Biotage offers a range of separation and synthesis technologies
across the three product areas Organic Chemistry, Analytical
Chemistry, and Industrial Products to a global market. The
Biotage products rationalize the workflow of customers and
reduce their impact on the environment, for example by using
a lower volume of solvents. Customers use Biotage products
in their development of new medicines and to analyze
samples from hospital patients, in forensic laboratories, or
for the analysis of environmental and food samples. Biotage
also offers products to remove undesired substances from
pharmaceuticals during the manufacturing process.
Chief Chromatographic Techniques Supported ⦁ Sample Prep
⦁ Flash Chromatography
⦁ Protein Precipitation
⦁ Supported Liquid Extraction
⦁ Solid Phase Extraction
⦁ Filtration
⦁ Automation
⦁ Evaporation
⦁ Microwave Synthesis
⦁ Peptide Synthesis
Markets Served Clinical, Forensic, Tox, Pharmaceutical, CRO, Biotech, Food,
Agricultural, Environmental, Government, Chemicals, Educa-
tion, Natural Products, Petroleum
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 13www.chromatographyonline.com
Major Products/Services 6th generation Pyroprobe 6000 series, 2nd
generation Purge and Trap autosampler
7350/7450 with 7000E concentrator, 2nd
generation Thermal Desorption autosampler
7550 with 9300 series concentrator, 2nd
generation Thermal Desorption stand-alone
autosampler 7550S with Peltier supported
Facility CDS’s state-of-art 10,000 sq. ft. facility
in Oxford, Pennsylvania, houses
manufacturing, customer support,
engineering, service and administrative
personnel in one building, where we
design, build, assemble, and analytical
test everything in house. Our production
utilizes six sigma lean manufacturing
processes and is delivering the products
with the highest quality in the industry.
AnalyticalCDSCDS
CDS Analytical
Company Description CDS Analytical is a leading global provider of innovative ther-
mal sample preparation instrumentation for the analytical
laboratory. For over 45 years our exclusive focus has been on
conceiving, designing, manufacturing, and supporting leading
edge instruments. CDS offers a complete suite of diverse front-
end GC equipment including pyrolyzers, purge and trap, head-
space, and thermal desorption systems. With a well-established
leadership role with pyrolyzers and other introduction systems,
as well as broad market penetration on a global scale, CDS
continues to be innovative and dynamic, and will pioneer the
next wave of sample introduction instrumentation.
Chief Chromatographic Techniques Supported⦁ Pyrolysis
⦁ Purge and Trap
⦁ Dynamic Headspace
⦁ Thermal Desorption
Markets Served CDS Analytical products have been serving but not limited to the
following markets as: Polymer, Environmental, Aerospace, Agri-
culture, Automobile, Chemical Weapon, Energy, Food and Flavor,
Forensics, Paper & Ink, Pharmaceuticals, Semiconductor, Textiles,
and Tobacco.
CDS Analytical
465 Limestone Road,
Oxford, PA 19363
T E L E P H O N E
(800) 541-6593
F A X
(610) 932-4158
E - M A I L
W E B S I T E
www.cdsanalytical.com
N U M B E R O F E M P L O Y E E S
23
Y E A R F O U N D E D
1969
14 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Major Products/Services CEM has recently launched a revolutionary
new system for LC–GC sample prep.
The EDGE Energized Dispersive Guided
Extraction system provides fully automated
extraction in as little as 5 min; including
filtration, cooling, and system washing.
The EDGE combines the processes
of Pressurized Fluid Extraction with
Dispersive Solid Phase Extraction in a
single instrument to yield fast results,
regardless of sample size, matrix, or use
of sorbents. The EDGE supports a wide
variety of applications including pesticides
in foods, total fat extraction, phthalates
in plastics, and environmental extractions
such as EPA 3545.
Facility CEM’s global headquarters, research
laboratories, and manufacturing facilities
are located in Matthews, North Carolina.
The company has subsidiaries in the
United Kingdom, Ireland, Germany, Italy,
France, and Japan as well as more than 50
distributors worldwide.
CEM Corporation
Company Description CEM Corporation is a leading global company specializing
in scientific solutions for critical laboratory applications. We
design and manufacture systems for analytical laboratories,
bioscience applications, life sciences, and processing plants
worldwide. Our product portfolio includes innovative
instrumentation for sample preparation for elemental or
chromatographic analysis, chemical synthesis, peptide
synthesis, and other applications.
Chief Chromatographic Techniques Supported ⦁ LC
⦁ LC–MS
⦁ GC
⦁ GC–MS
Markets Served CEM instrumentation is used by chemists, scientists, and
technicians in private industry as well as leading universities,
analytical laboratories, and research facilities around
the world. We support numerous markets including the
pharmaceutical, chemical, environmental, applied materials,
food, and petroleum industries, among others.
CEM Corporation
PO Box 200,
Matthews, NC 28106
T E L E P H O N E
(704) 821-7015
(800) 726-3331
F A X
(704) 821-7894
E - M A I L
W E B S I T E
www.cem.com
N U M B E R O F E M P L O Y E E S
USA: 245
Elsewhere: 65
Y E A R F O U N D E D
1978
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 15www.chromatographyonline.com
Major Products/Services PAL System is the tool box for sample
preparation, from a simple liquid injection
to complete workflows. A PAL System can
be adapted or extended to meet almost
any requirement.
CTC Analytics
Company Description CTC Analytics, a privately owned Swiss company, is leading
in front-end automation for gas and liquid chromatography.
Over the last 30 years, investments in the continuous devel-
opment of a flexible, highly reliable, advanced laboratory sam-
ple handling platform have been made, primarily designed for
use in the pharmaceutical, life science, chemical, environmen-
tal and food & flavor industries.
Our robotic autosampler systems have a flexible modular
design, allowing our worldwide customer base to create their
own customized solution to respond to their individual needs. All
products are distributed through OEM channels and a selected
network of independent CTC distributors around the world.
Chief Chromatographic Techniques Supported ⦁ GC
⦁ GC–MS
⦁ LC
⦁ LC–MS
Markets Served ⦁ Environmental
⦁ Food
⦁ Pharmaceutical
⦁ Chemical
⦁ Forensic
⦁ Clinical
CTC Analytics
Industriestrasse 20, 4222
Zwingen, Switzerland
T E L E P H O N E
+41 61 765 8100
E - M A I L
W E B S I T E
www.palsystem.com
Y E A R F O U N D E D
1985
16 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Chem Service, Inc.
660 Tower Lane,
West Chester, PA 19381
T E L E P H O N E
(800) 452-9994
F A X
(610) 692-8729
E - M A I L
W E B S I T E
www.chemservice.com
Y E A R F O U N D E D
1962
Chem Service, Inc.
agencies; food quality control; agricultural
testing and research; life science endeavors;
environmental control and remediation;
college and university work; and the
manufacture of chemicals, pulp and paper,
and alcoholic beverages.
Major Products/Services ⦁ Flame retardants/PBDE
⦁ Metabolite standards
⦁ EPA 535 ESA/OA metabolites
⦁ Explosive residues
⦁ PCB congeners
⦁ Petroleum hydrocarbons
⦁ Fatty acid methyl esters
⦁ High purity inorganics
⦁ Surfactants
⦁ Anti-oxidants
⦁ Ultraviolet inhibitors
⦁ Plasticizers
⦁ Biological stains
⦁ Phthalate standards
⦁ Polynuclear aromatics
⦁ Vitamins
⦁ Carbohydrates standards
⦁ Forensic standards
⦁ Personal care product standards
⦁ Pesticide residue standards
Facility Chem Service’s headquarters are located in
West Chester, Pennsylvania. A global network
of distributors for sales outside the United
States is available for all of our products.
Company Description Established in 1962, Chem Service is accredited for ISO Guide
34:2009 and ISO/IEC 17025:2005 and registered and certified
to the ISO 9001:2008 Quality Management System for the
design, development, production, distribution, and servicing of
organic neat and synthetic reference materials. Chem Service, Inc.
produces high purity chemicals for use as reference materials and
for other laboratory purposes. More than 95% of their standards
grade materials have a certified purity of 98.0% or greater and
do not require purity corrections when preparing solutions for
use with EPA, USTM, UST, and numerous other international
methods. Standards grade chemicals are clearly labeled with an
expiration date that is based on years of experience in handling
and testing. Products are packaged in small quantities to
minimize storage, waste, and disposal requirements. Organic and
inorganic chemicals, solutions, and mixtures are available to meet
a wide range of specialized laboratory needs.
Chief Analytical Techniques Supported⦁ GC–FID
⦁ GC–ECD
⦁ GC–TCD
⦁ GC–MS
⦁ HPLC
⦁ FT–IR
⦁ UHPLC–MS
Markets Served Chem Service has thousands of worldwide customers in a
wide range of sectors, including government and regulatory
chemservice.com | [email protected] | 1-800-452-9994
QUALITY
,62Guide 34 - 9001 - 17025
Pesticide and Metabolite Standards CatalogPPeessticiide and Metabolite Standards CaatallooggPPeessticiide and Metabolite Standards Caataloogg
REFERENCESTANDARDS
CERTIFIED
Over 2,000 products
Cannabis StandardsNeonicotinoidsPBDEsPhthalatesVitaminsExplosivesand more!ł
ł
ł
ł
ł
ł
ł
18 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
FMS, Inc.
580 Pleasant Street
Watertown, MA 02472
T E L E P H O N E
(617) 393-2396
F A X
(617) 393-0194
E - M A I L
W E B S I T E
www.fms-inc.com
N U M B E R O F E M P L O Y E E S
90
O U T S I D E U S
4
Y E A R F O U N D E D
1985
FMS, Inc.
Chief Chromatographic Techniques Supported ⦁ Automated sample preparation
⦁ Sample extraction
⦁ Pressurized liquid extraction
⦁ Solid phase extraction
⦁ Automated fractionation
⦁ Sample cleanup
⦁ Automated sample cleanup
⦁ Multicolumn cleanup
⦁ Concentration
⦁ Evaporation
Markets Served ⦁ Agricultural
⦁ Chemical
⦁ Clinical
⦁ Coatings
⦁ Cosmetics
⦁ Energy
⦁ Environmental
⦁ Flavors
⦁ Food
⦁ Life science
⦁ Pharmaceutical
⦁ Petrochemical
⦁ Tobacco
⦁ Toxicology
⦁ Utilities
Facility Based in Watertown, Massachusetts,
just outside Boston, FMS, Inc. owns and
operates out of its 150,000 sq. ft facility on
the banks of the Charles River. All FMS, Inc.
products are proudly manufactured in the
US. The facility houses sales, marketing,
manufacturing, a machine shop, ISO
17925 accredited laboratory, and R&D.
Company Description Founded in 1985, Fluid Management Systems, Inc. designs,
manufactures, and supports analytical instruments used
by scientists to perform extraction, cleanup, fractionation,
and concentration of samples prior to chemical analysis.
Some of the industries that rely on our sample preparation
systems are: agricultural, chemical, clinical, coatings,
cosmetics, energy, environmental, flavors, food, life science,
pharmaceutical, petrochemical, tobacco, toxicology, and
utilities. Automated sample preparation systems from
FMS are designed to replace outdated, labor-intensive
techniques that hinder laboratory productivity. Our Total
Sample Preparation systems improve the efficiency of both
laboratory personnel and expensive instrumentation. Our
automated sample preparation systems allow laboratory
personnel to reduce costs, shorten sample turn around
time, and improve the quality and consistency of their
results by eliminating the variability inherent with manual
sample preparation methods. All FMS consumables are
guaranteed and manufactured in Class 10,000 and Class
1000 cleanroom environments and both our instruments
and consumables are proudly made in the US. We support
our sample preparation systems with an ever-increasing
network of field sales, service, and applications expertise.
FMS systems are sold worldwide by a direct sales force,
independent representatives, and distributors. After 25 years
in business, FMS has established itself as the world leader in
providing automated sample preparation systems in the field
of persistent organic pollutants (POPs) analysis.
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 19www.chromatographyonline.com
Fritsch Milling & Sizing, Inc.
Company Description FRITSCH is an internationally recognized manufacturer of
application-oriented laboratory instruments for sample
preparation and particle sizing.
Chief Chromatographic Techniques SupportedLaboratory Mills for optimal sample preparation of
chromatographic techniques
Markets Served Worldwide in the following industries: Mining, Construction
materials, Biology, Chemistry, Mechanochemistry,
Agriculture and forestry, Geology/mineralogy, Ceramics/glass
industry, Plastics/textiles, Foodstuffs,
Metallurgy, Environment, Pharmaceuticals
Major Products/Services Particle size reduction, milling, grinding, and analysis of
particle size and shape.
Facility USA headquarters and applications laboratory in North
Carolina. Global headquarters in Germany. Sales offices in
China, Asia Pacific, Russia, and France.
Fritsch Milling
& Sizing, Inc.
57 Grant Drive Suite G,
Pittsboro, NC 27312
T E L E P H O N E
(919) 229-0599
E - M A I L
W E B S I T E
www.fritsch-us.com
N U M B E R O F E M P L O Y E E S
100+ worldwide
Y E A R F O U N D E D
1920-Fritsch GmbH
20 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
pH stability (0–14) with the pressure stabil-
ity of silica-based columns. With Hamilton’s
Polymeric Reversed Phase (PRP™) HPLC
columns and resins, the sample dictates the
necessary separation conditions, not the
limitations of the column. Hamilton can also
customize HPLC columns to meet a desired
application need. Each product is manufac-
tured to achieve the highest level of accuracy.
Chromatography Syringes – Hamilton of-
fers the most complete selection of syringes
on the market for use in various applications
including GC and HPLC (autosamplers and
manual injection), thin layer chromatogra-
phy (TLC), liquid handling, and life sciences.
Crafting exceptional syringes is an evolving
science, which is why Hamilton is dedicated
to the continuous research and development
of this product line. Hamilton constantly en-
riches its entire syringe offering by either im-
proving existing models or introducing new
ones. Using customers’ needs and feedback
as a guide, we innovate in ways that maxi-
mize flexibility, performance, and value.
Microlab® 600 Diluter/Dispenser – The
Microlab® 600 is a highly precise syringe
pump with a touchscreen interface designed
to quickly and easily dilute and dispense flu-
ids, while increasing throughput and reduc-
ing cost and wasted buffer. This positive dis-
placement system provides better than 99%
accuracy, independent of a liquid’s viscosity,
vapor pressure, and temperature. The inert
fluid path minimizes sample carryover and is
compatible with harsh chemicals.
Facilities Hamilton Company is a global enterprise with
headquarters in Reno, Nevada; Hopkinton,
Massachusetts; and
Bonaduz, Switzer-
land, and subsidiary
offices throughout
the world.
Hamilton CompanyChromatography Products for HPLC and GC
Company Description Hamilton Company is an industry leader in the design and
manufacture of liquid handling, process analytics, robotics, and
automated storage solutions. For 60 years, Hamilton has been
satisfying customer needs by combining quality materials with
skilled workmanship to ensure the highest level of performance.
Hamilton’s lifelong commitment to precision and quality has
earned a global ISO 9001 Certification.
Founded on the technology of analytical Microliter™ and
Gastight® syringes, Hamilton has a broad offering of laboratory
products including manual and semi-automated precision fluid
measuring instruments, chromatography products, process
sensors, laboratory electrodes, pipettes, and more.
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ Polymeric HPLC columns: reversed phase, anion exchange,
cation exchange, and ion exclusion
⦁ Silica HPLC columns
⦁ GC septa
⦁ Chromatography syringes (HPLC and GC)
Markets Served ⦁ Analytical
⦁ Pharmaceutical
⦁ Biotechnology
⦁ Industrial
⦁ Clinical
⦁ Environmental
Major Products/Services HPLC Columns – Hamilton manufactures 18 types of polymeric
HPLC columns for reversed phase, anion exchange, cation ex-
change, and ion exclusion separations, providing a wide range
of retention characteristics and performance benefits. Hamilton’s
polymer-based HPLC columns provide maximum inertness and
Hamilton Company
4970 Energy Way
Reno, NV 89502
T E L E P H O N E
(775) 858-3000
(800) 648-5950
F A X
(775) 856-7259
E - M A I L
W E B S I T E
www.hamiltoncompany.com
Y E A R F O U N D E D
1953
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 21www.chromatographyonline.com
Major Products/Services Below is a sample of some HPLC column
phases from Imtakt:
⦁ Dacapo DX-C18: 2.5 μm particle C18 col-
umn designed with a revolutionary dual
matrix particle technology, optimized for
extreme pH conditions.
⦁ Intrada Amino Acid: The world’s first
mixed-mode column designed to sepa-
rate amino acids without the need for any
derivatization.
⦁ Intrada SEC: Silica based SEC column
designed for use in SEC-MS compatible
conditions. GPC and GFC.
⦁ SCHERZO SM-C18: C18 multi-mode
column with both anion and cation ex-
change.
⦁ PRESTO FF-C18: Consists of C18 bonded
to monodispersed 2 μm non-porous
silica. It is useful for both biopolymer and
synthetic polymer separations. Ideal for
intact antibody and ADC analysis.
⦁ CADENZA CD-C18: High resolution; 25–
50% lower pressure; best with hydropho-
bic compounds, molecular recognition,
and small molecule isomer separation.
⦁ Cadenza HS-C18: High resolution C18
column designed to accept direct injec-
tion of biological samples. No need to
protein precipitate your samples for high
throughput early drug discovery or clinical
lab testing.
⦁ UNISON UK-AMINO: Revolutionary aque-
ous durability for aminopropyl phase;
aqueous to non-aqueous normal phase
separation.
Facility We are located in Portland, Oregon, and
deliver next day for 95% of North Ameri-
can orders and always within one week.
Imtakt USA
Company Description Our HPLC columns have been designed and manufactured
by Imtakt Corporation with exceptional craftsmanship and
decades of industry experience. Truly some of the most unique
column chemistries in the market today. Our columns give
chromatographers:
⦁ High resolution
⦁ 25–50% lower pressure
⦁ Novel chemistry
Chief Chromatographic Techniques Supported⦁ High performance liquid chromatography
⦁ Ultra high pressure liquid chromatography
⦁ Liquid chromatography–mass spectrometry
⦁ Reverse phase
⦁ Normal phase
⦁ Anion exchange
⦁ Cation exchange
⦁ Multi-mode
⦁ Small molecule separations
⦁ Protein separations
Markets Served All LC and LC–MS markets including: pharmaceutical, biotech,
contract research, forensic, clinical diagnostic, food and
beverage, industrial, chemical, environmental, and academic
research.
Imtakt USA
2892 NW Upshur St.
Portland, OR 97210
T E L E P H O N E
(215) 665-8902
F A X
(501) 646-3497
E - M A I L
W E B S I T E
www.imtaktusa.com
Y E A R F O U N D E D
2004
22 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Products include:
⦁ Pegasus BT® GC-TOFMS
The tried-and-tested reliability and
durability of our Pegasus brand in a
convenient benchtop unit
⦁ Pegasus 4D-C GC×GC-TOFMS
Four dimensions of analytical resolu-
tion offer more complete analysis of the
most complex samples
⦁ Pegasus GC-HRT+
GCMS with industry-leading resolution
(up to 50,000) and mass accuracy for
high-information content analysis
⦁ Pegasus GC-HRT+ 4D
Combining the highest performance
GCxGC and TOFMS on the market with
High Resolution Deconvolution®(HRD®)
Facility LECO’s global headquarters in St. Joseph,
Michigan include the Elizabeth S. Warren
Technical Centre, a 28,000 square-foot
facility exclusively dedicated to the research
and development of innovative instrumen-
tation and equipment for LECO’s separation
science line. Customer training demonstra-
tions and application support are available
at our Life Science and Chemical Analysis
Centre in St. Joseph. LECO has more than
25 subsidiaries worldwide, plus additional
international distributors.
LECO Corporation
Company Description For over 80 years, industries around the world have trusted
LECO to deliver technologically advanced products and
solutions for analytical science. Today’s technologies for
separation science answer key questions in complex samples
and pioneer high sample throughput using GCxGC, GCxGC-
TOFMS, GC-TOFMS, and high resolution GCxGC-TOFMS. All
instrumentation includes LECO’s exclusive ChromaTOF®
operating software for seamless data handling.
Chief Chromatographic Techniques Supported ⦁ GC-TOFMS
⦁ High Resolution GC-TOFMS
⦁ GCxGC-FID/ECD
⦁ GCxGC-TOFMS
⦁ High Resolution GCxGC-TOFMS
Markets Served LECO Separation Science instruments are used in a variety
of applications, helping users to uncover unidentified
compounds in their samples. Markets include metabolomics,
consumer product safety, food, flavor, fragrance,
environmental, and energy and fuels analysis.
Major Products/Services An ideal choice for laboratories investigating highly complex
samples, LECO products feature exclusive ChromaTOF brand
software with all the tools needed to easily locate, identify,
and quantify both target and unknown analytes, dramatically
reducing overall analysis times and increasing productivity.
LECO Corporation
3003 Lakeview Avenue
St. Joseph, MI 49085
T E L E P H O N E
(800) 292-6141
(269) 985-5496
F A X
(269) 982-8987
E - M A I L
W E B S I T E
www.leco.com
N U M B E R O F E M P L O Y E E S
717
Y E A R F O U N D E D
1936
Agilent Technologies is offering five years complimentary access to
CHROMacademy for all university students and staff.
CHROMacademy is an intuitive, comprehensive e-learning and trouble-
shooting platform with more than 3,000 pages of content for HPLC,
GC, sample preparation, and hyphenated techniques. No other online
resource offers separation scientists more live streaming events, a
knowledge base, practical solutions, and new technologies in one easy
to navigate website.
Get your free five year membership worth US $1,995* by submitting the
form at www.chromacademy.com/agilent.
* Five years free access to CHROMacademy only available to customers affiliated with an academic
or research institution, conditions apply. A valid university e-mail address if required.
© Agilent Technologies, Inc. 2017
CHROMACADEMY MEMBERSHIP
FREE
24 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
MACHEREY-NAGEL INC.
2850 Emrick Blvd.,
Bethlehem, PA 18020
T E L E P H O N E
(484) 821-0984
F A X
(484) 821-1272
E - M A I L
W E B S I T E
www.mn-net.com
N U M B E R O F E M P L O Y E E S
ca. 620
(Worldwide)
Y E A R F O U N D E D
1911
optimal and reliable solutions for analytical
task in method development and routine
analysis.
Major Products/Services NUCLEOSIL®, one of the first spherical silica
gels in the 1970s, is still packed in stainless
steel columns by MN. These columns with
various C18
modifications are reliable work-
horses and are validated in many drug test-
ing methods. In recent years new highly pure
and ultra-spherical HPLC and UHPLC silica
gels followed. Today, the product range of
totally porous NUCLEODUR® and fused-core
NUCLEOSHELL® columns comprise more
than 25 stationary phases, targeted to daily
routine analysis as well as for method devel-
opment in HPLC. More information:
www.mn-net.com/HPLC
MACHEREY-NAGEL, a pioneer in Thin-
Layer Chromatography, is still manufactur-
ing glass plates as well as aluminum and
plastic sheets coated with silica gels and
other sorbents for TLC and HPTLC. More
information: www.mn-net.com/TLC
Classical CHROMABOND® silica gel
phases with various modifications (such
as C18
, C8, NH
2, OH, CN) and modern poly-
meric resins are widely used for sample
preparation in environmental analysis, food
safety control, or clinical applications. The
hydrophilic-lipophilic balanced polymeric
SPE adsorbent CHROMABOND® HLB and
the CHROMABOND® HR-Xpert line of poly-
styrene-divinyl based RP and mixed-mode
ion-exchange resins offer a full toolbox of
innovative SPE products. More information:
www.mn-net.com/SPE
Facility The headquarters in Germany and
branches in the United States, Switzerland
and France, as well as a globally operating
network of distributors in more than 150
countries, ensure worldwide availability of
MN products and services.
MACHEREY-NAGEL GMBH & CO. KG
Company Description Since 1911, MACHEREY-NAGEL represents high quality, in-
novation, and reliability in analytical chemistry and life science.
Friendly expert advice and outstanding product quality have been
MN´s mission for over 100 years as a family-owned business in
the fourth generation. As one of today’s leading manufacturers,
MACHEREY-NAGEL offers a broad range of products for chroma-
tography, filtration, rapid tests, water analysis, and bioanalysis.
MN´s expertise in manufacturing of chromatography media
is based on many years of experience in silica technology. The
development of the spherical HPLC silica NUCLEOSIL® and the
introduction of commercially available ready-to-use TLC plates
and sheets are just two milestones emphasizing the pioneering
tasks of MN in promoting these separation techniques, which
today are omnipresent in analytical labs all over the world.
Chief Chromatographic Techniques Supported ⦁ SPE and Flash Chromatography
⦁ HPLC
⦁ TLC
⦁ GC
⦁ Chromatographic accessories (such as syringe filters, vials,
and caps)
Markets Served MN serves laboratories all over the world with HPLC, GC, and SPE
columns, TLC plates and sheets, syringe filters, or suitable vials
and caps. Customers from many different industries (such as the
food and beverage industry, pharmaceutical industry and health-
care, chemical industry, institutes for environmental analysis,
biotechnology, universities, and research institutes) benefit from
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 25www.chromatographyonline.com
introduced many highly successful products
to the analytical laboratory:
⦁ UNITY-xr: A universal thermal desorption
unit for sorbent sampling tubes
⦁ TD100-xr: A fully automated TD system
for sorbent tubes
⦁ ULTRA-xr series of TD autosamplers
⦁ CIA Advantage-xr systems for auto-
mated cryogen-free analysis of air and
gas sampled using canisters
⦁ TT24-7xr for continuous air monitoring
⦁ The Micro-Chamber/Thermal Extractor
for fast sampling of emissions from
products and materials
⦁ Wide range of accessories and
consumables for TD
Markes also manufactures the BenchTOF
range of time-of-flight mass spectrometers
and associated software. Markes’
technological innovation for mass
spectrometry also includes Select-eV®
(ground-breaking ion-source technology)
and Tandem Ionisation®, for BenchTOF.
FacilitiesMarkes International’s factory, technical
center, and headquarters are located
near Cardiff, UK. The company also has
technical centers in Frankfurt, Germany,
Shanghai, China, and Gold River, California.
Markes also supports a global distributor
network. Markes is a company of the
Schauenburg International Group.
Markes International
Company DescriptionMarkes International, an industry leader in technology for trace
organic analysis, manufactures a range of instrumentation and
software that enhances the analytical capability and productivity
of GC–MS systems.
Chief Chromatographic Techniques Supported ⦁ Thermal desorption (TD) as a sample concentration and
introduction technique for GC
⦁ Time-of-flight mass spectrometry (TOF-MS) for GC and
GC×GC
Markets Served ⦁ Environmental
⦁ Consumer environmental health
⦁ Defense and homeland security and forensic
⦁ Food and beverage
⦁ Fragrance and odor profiling
⦁ Biological profiling
⦁ Petrochemical
Markes’ global customer base includes major industry,
government agencies, academia, and the contract service
laboratory sector.
Major Products/ServicesMarkes is globally recognized for its innovation, high-quality
products, unrivalled technical expertise, and high level of
customer service within the field of analytical TD. As a global
technology leader of thermal desorption for GC, Markes has
Markes International, Inc.
2355 Gold Meadow Way
Gold River, CA 95670
T E L E P H O N E
(866) 483-5684 (toll free)
Markes International Ltd.
Gwaun Elai Medi-Science
Campus, Llantrisant, RCT,
CF72 8XL, UK
T E L E P H O N E
+44 (0)1443 230 935
Markes International
GmbH
Schleussnerstrasse 42,
D-63263 Neu-Isenburg,
Frankfurt, Germany
T E L E P H O N E
+49 (0)6102 8825569
E - M A I L
W E B S I T E
www.markes.com
NUMBER OF EMPLOYEES: 110
Y E A R F O U N D E D
1997
26 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
MicroSolv Technology Corporation
9158 Industrial Blvd. NE
Leland, NC 28451
T E L E P H O N E
(732) 380-8900
F A X
(910) 769-9435
E - M A I L
W E B S I T E
www.mtc-usa.com
N U M B E R O F E M P L O Y E E S
Under 25
Y E A R F O U N D E D
1992
MicroSolv Technology Corporation
Facilities Production facility and technical support
laboratory is located in upstate New
York and headquarters are in the greater
Wilmington, North Carolina area.
Company Description A privately held firm in the greater Wilmington, NC area, that
acquires, develops, and manufactures new technologies
and products for separation sciences. They serve pharma,
chemistry, medical and clinical R&D, environmental,
forensic, and biological laboratories worldwide through their
distribution network.
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ LC–MS
⦁ Capillary electrophoresis
Markets Served All analytical and biology laboratories that use HPLC, LC–MS,
GC, GC–MS, SFC, or CE in all markets segments and in all
countries around the world.
Major Products/Services Silica hydride based HPLC columns under the Cogent TYPE-C
Silica brand name, the RSA brand autosampler vials
(reduced surface activity), and the CElixir brand of dynamic
coatings for CE. Online community serves chromatographers
and provides a forum for sharing ideas and problem solving.
ES996842_LCGCSUPP1217_026.pgs 12.07.2017 17:22 ADV blackyellowmagentacyan
Discover the Diff erence…
» Detect low abundance analytes normally adsorbed by glass.
» Prevents pH changes in vials before injection even hours later.
» Prevents sample hydrolysis that can occur in your vials.
» No meniscus when water is used as the main solvent.
MANUFACTURED BY:
www.mtc-usa.com
Know What You
DIDN’T KNOW
» Polar and non-polar compounds can be separated in the same
isocratic run.
» Precision run to run, day to day, batch to batch.
» Equilibration time is much faster due to the diff erence in the
adsorption layer due to the weak attraction of the silica.
ANP
Replaces
HILIC
» Thermal Control
» Compatibility
» Orthogonality
» Sustainability
NEW
SAMPLE
MANAGEMENT
SYSTEM
28 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Major Products/Services Our Milli-Q® Lab Water purification system
portfolio offers a broad range of pure and
ultrapure water purification systems and
services designed for scientists working
in both validated and non-validated
environments.
Facility The Life Science business of Merck KGaA,
Darmstadt, Germany, which operates as
MilliporeSigma in the U.S. and Canada,
has 60 manufacturing sites worldwide.
MilliporeSigma, the vibrant M and
Milli-Q are trademarks of Merck KGaA,
Darmstadt, Germany or its affiliates.
All other trademarks are the property
of their respective owners. Detailed
information on trademarks is available
via publicly accessible resources.
MilliporeSigma
Company Description The Life Science business of Merck KGaA, Darmstadt,
Germany, which operates as MilliporeSigma in the U.S. and
Canada, has 20,000 employees, with a portfolio of more than
300,000 products enabling scientific discovery. Udit Batra is
the global chief executive officer of MilliporeSigma.
Merck KGaA, Darmstadt, Germany completed its $17 billion
acquisition of Sigma-Aldrich in November 2015, creating a
leader in the $125 billion global life science industry.
Merck KGaA, Darmstadt, Germany is a leading company for
innovative and top-quality high-tech products.
Chief Chromatographic Techniques Supported ⦁ Analytical HPLC
⦁ Gas Chromatography
⦁ Ion Chromatography
⦁ TLC and HPTLC
⦁ Mobile Phases for Chromatography
Markets Served ⦁ Pharmaceutical
⦁ Clinical
⦁ Academic
⦁ Industrial
⦁ Research
⦁ Government Laboratories
MilliporeSigma
400 Summit Drive,
Burlington, MA 01803
T E L E P H O N E
+1 (800) 645-5476
E - M A I L
W E B S I T E
www.emdmillipore.com
N U M B E R O F E M P L O Y E E S
20,000
Y E A R F O U N D E D
1668
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 29www.chromatographyonline.com
Mott Corporation
84 Spring Lane
Farmington, CT 06032
T E L E P H O N E
(860) 747-6333
F A X
(860) 747-6739
E - M A I L
W E B S I T E
www.mottcorp.com
Y E A R F O U N D E D
1959
Mott Corporation
Major Products/Services Mott specializes in the use of porous
metal and 3D printing for the following
fluid control products: industrial filters,
high purity filters, solvent sinker filters,
inline filters, static mixers, column frits,
flow restrictors, industrial spargers, micro-
spargers, and various other filtration and
flow control assemblies. Each solution is
customizable to meet the specifications
of your system. In addition, Mott offers
the following engineering services: rapid
prototyping, world class product design
consultation, and state-of-the-art lab
testing and support.
Facility Mott’s headquarters and manufacturing
facility operate in two adjacent facilities in
Farmington, Connecticut. Manufacturing
facility includes both a Class 100 and Class
10,000 cleanroom for applications with
high cleanliness requirements. Mott has
remote offices in various countries and a
global distribution network.
Company Description Mott is a high precision filtration and flow control company
trusted by the world’s best known technical and performance
brands. Often, our customers have no effective options to
solve their challenging problems and turn to us for solutions.
Our strategic vision is to deliver technological breakthroughs
where none currently exist.
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ UHPLC
⦁ LC–MS
⦁ GC
Markets Served Mott provides various product solutions and design
consultation support to the following industries: Analytical
instrumentation, pharmaceutical, biotechnology, food &
beverage, medical, microelectronics, chemical processing,
aerospace, environmental, process control, and energy.
30 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
We also offer an integrated program called
Laboratory Equipment Asset Procurement
Services (LEAPS) that provides new and
existing customers within the global
science market with convenient access
to end-to-end laboratory services and
insights. It combines OEM contract
administration, parts and relocation
services, and reporting with tier 1
diversity spend and cost savings to drive
operational efficiencies and economic
outcomes nationwide.
Markets Served Neta Scientific serves research and
development labs throughout North
America, encompassing the pharmaceutical,
biotech, life sciences, academia and research
institutions, government facilities, food and
flavors, industrial testing, and chemical
industries.
Facility The corporate offices and main warehouse
are headquartered in Hainesport, New
Jersey. We also maintain additional
representation throughout the United States.
Neta Scientific Inc.
Company Description Neta Scientific is a laboratory supplies and solutions company
that enables the global science and clinical market to more
effectively conduct world-leading research and development.
We centralize superior supplies, services, and supply chain
management with a customer-centric approach, innovative
technologies and diversity certifications for customers to easily
gain access to the tools needed to improve global health and
safety and meet corporate objectives.
Major Products/Services At Neta Scientific, we enable your mission by centralizing
superior laboratory supplies and supply chain management
with our unique ability to provide pass-through support
for major brand suppliers, as well as OEM contract, parts,
consumables, and services to give you back the time you
need.
⦁ Chromatography
⦁ Life Sciences
⦁ Labware
⦁ Chemicals & Reagents
⦁ Services (LEAPS)
⦁ Small & Large Bench Instruments
⦁ Cold Storage
Neta Scientific Inc.
4206 Sylon Blvd.,
Hainesport, NJ 08036
T E L E P H O N E
(800) 343-6015
F A X
(609) 265-8213
E - M A I L
W E B S I T E
www.netascientific.com
N U M B E R O F E M P L O Y E E S
40+
Y E A R F O U N D E D
1999
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 31www.chromatographyonline.com
Parker Hannifin
Corporation
Gas Separation and
Filtration Division
4087 Walden Avenue
Lancaster, NY 14086
T E L E P H O N E
(978) 858-0505
F A X
(978) 556-7501
W E B S I T E
www.labgasgenerators.com
N U M B E R O F E M P L O Y E E S
55,000
Y E A R F O U N D E D
1924
Parker Hannifin Chief Chromatographic Techniques Supported ⦁ GC–FID, FPD, NPD
⦁ GC–HALL, ECD, TCD, HST-Wire
⦁ Capillary separations
⦁ Packed separations
Markets Served ⦁ Agriculture
⦁ Biotechnology
⦁ Chemicals
⦁ Chemical and explosives detection
⦁ Energy
⦁ Environmental
⦁ Inorganic chemicals
⦁ Instrument development
⦁ Life science
⦁ Organic chemicals
⦁ Paints and coatings
⦁ Petrochemicals
⦁ Pharmaceuticals
⦁ Plastics
Major Products/Services Gas generators for the following analytical
instruments:
⦁ Gas chromatographs
⦁ LC–MS
⦁ FT-IR spectrometers
⦁ ICP emission spectrometers
⦁ TOC analyzers
⦁ Atomic absorption spectrophotometers
⦁ Nuclear magnetic resonance (NMR)
⦁ Rheometers/thermal analyzers
⦁ Sample evaporators/concentrators
Facilities Parker Hannifin manufactures all gas genera-
tors in Haverhill, Massachusetts. Parker has
over 50,000 generators installed globally and
distribution points worldwide.
Company Description Safety: Parker Balston gas generators completely eliminate the
safety hazards involved with handling high-pressure gas cylinders.
Enjoy hassle-free automation with no tanks to charge and no
downtime.
Reliability: Thousands of laboratories worldwide have
Parker Balston gas generators in routine use. Parker Balston
gas generators are recommended and used by major instru-
ment manufacturers. We offer the best technology at an af-
fordable price from the brand you trust.
Quality: Each Parker Balston gas generator is manufactured
under a strict total quality management program. We have a
world-class ISO 9001-certified manufacturing facility in the
United States. All Parker Balston gas generators are backed
by a complete satisfaction guarantee. Parker offers preventa-
tive maintenance, extended warranties, and field repair pro-
grams for all laboratory gas generators, as well as a network
of highly specialized sales, application, and technical support
people.
Products: Hydrogen gas generators produce 99.99999%
pure hydrogen for gas chromatographs. Zero air genera-
tors produce zero grade air for gas chromatographs. UHP
nitrogen generators produce 99.9999% pure nitrogen for
GCs or ICP spectrometers. FT-IR gas generators produce
dry, CO2-free purge gas for FT-IR spectrometers. Pure
air and nitrogen generators produce dry, ultrapure com-
pressed gas for laboratory instruments, including LC–MS
instruments.
32 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Major Products/Services PerkinElmer, Inc. offers a wide range of
instrumentation and solutions to meet
your analytical measurement needs:
⦁ Atomic spectroscopy: AA, ICP-OES,
ICP-MS
⦁ Chromatography: GC and GC custom
solutions, GC–MS, HPLC and UHPLC,
LC–MS
⦁ Hyphenated techniques: HPLC–ICP-MS,
GC–ICP-MS, HS-GC, HS-GC-MS, TD-GC,
TD-GC–MS, TG-IR, TG-MS, TG-GC–MS
⦁ Mass spectrometry: ICP-MS, GC–MS,
LC–MS
⦁ Molecular spectroscopy: FT-IR and
FT-NIR, UV-vis and UV-vis-NIR,
fluorescence spectroscopy
⦁ Thermal analysis: DSC, TGA, STA, DMA
⦁ Organic elemental analysis: CHN/O,
CHNS/O
⦁ Consumables: atomic spectroscopy,
chromatography, mass spectrometry,
molecular spectroscopy, thermal
analysis, elemental analysis
⦁ OneSource® Laboratory Services
Facility PerkinElmer, Inc. operates globally in 150
countries.
PerkinElmer, Inc.
Company Description PerkinElmer is a global leader focused on improving human
and environmental health, for the better. We provide our
customers with critical knowledge, expertise, and innovative
detection, imaging, software, and services so that they can
make better decisions for better outcomes. At PerkinElmer,
we make a difference every day helping scientists, clinicians,
and governments detect earlier and more accurately to
improve the health and safety of people and the environment.
Our solutions range from enabling the discovery of more
effective diagnostics and therapies, to making sure that the
food we eat, the water we drink, and our environment are
safe from contaminants.
Chief Chromatographic Techniques Supported⦁ Gas chromatography
⦁ GC–MS and portable GC–MS
⦁ Liquid chromatography—HPLC and UHPLC
⦁ Headspace and thermal desorption sample handling
⦁ LC–MS
Markets Served PerkinElmer is a leading provider of precision instrumenta-
tion, reagents and chemistries, software, and services for a
wide range of scientific and industrial laboratory applications,
including environmental monitoring, food and beverage qual-
ity/safety, and chemical analysis, as well as genetic screening,
drug discovery, and development.
PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451
T E L E P H O N E
(781) 663-6900
E - M A I L
CustomerCareUS@
perkinelmer.com
W E B S I T E
www.perkinelmer.com
N U M B E R O F E M P L O Y E E S
7700
Y E A R F O U N D E D
1937
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 33www.chromatographyonline.com
Markets Served Mass spectrometry, nuclear detection,
medical instrumentation, pharmaceutical
safety, industrial instrumentation, and image
intensification, as well as the custom design
and manufacture of detectors, sensors, and
ion mobility analyzers.
Major Products/Services PHOTONIS offers a complete range of
high performance scientific and medical
detector products. Our market includes
electron multipliers, microchannel plates,
mass spectrometry fiber optics and resistive
glass, advance performance time-of-flight
detectors, image intensifiers, ion mobility
analyzers, neutron imagers, and other related
products. Our detection products are found
in most of today’s technology-based markets,
including analytical instrumentation, medical
diagnostics, chemistry, drug discovery,
high-energy physics, space exploration, and
scientific research. PHOTONIS is the largest
supplier of standard, retrofit, and custom
detectors in the mass spectrometry, residual
gas analyzer, and electron microscope
markets providing advanced detector designs
for the highest sensitivity through superior
signal collection and noise reduction.
Facilities Sturbridge, MA; Lancaster, PA; Frisco, TX;
Brive, France; Roden, Netherlands.
PHOTONIS USA
Company Description PHOTONIS is a multinational high-technology group, with
more than 40 years of experience in manufacturing, sales, and
innovation, specializing in charged particle and photon sensor
technology. The group operates internationally in the night vision,
industrial, scientific, and medical markets.
Chief Chromatographic Techniques Supported ⦁ Liquid chromatography detectors
⦁ Ion mobility spectrometry
⦁ Mass spectrometers
⦁ UV–vis fixed wavelength
⦁ UV–vis variable wavelength
⦁ Gas chromatography detectors and accessories
⦁ Detector accessories
⦁ Mass spectrometers: high-resolution
⦁ Mass spectrometers: low-resolution
⦁ Mass spectrometers, residual gas analysis, magnetic
⦁ Mass spectrometers, residual gas analysis, quadrupole
⦁ ICP-MS
⦁ Time-of-flight mass spectrometry
⦁ Other detectors and accessories
⦁ Supercritical fluid chromatography detectors and accessories
⦁ Radioactivity
⦁ Neutron imaging
PHOTONIS USA
660 Main Street
Sturbridge Business Park
Sturbridge, MA 01566
T E L E P H O N E
(508) 347-4000
F A X
(508) 347-3849
E - M A I L
W E B S I T E
www.photonis.com
N U M B E R O F E M P L O Y E E S
US: 150
Elsewhere: 900
Y E A R F O U N D E D
1937
34 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
⦁ Medical analysis
⦁ Pharmaceutical analysis
Major Products/Services Reference Materials
⦁ GPC/SEC standards and kits
⦁ Certified reference materials
⦁ MALDI kits
⦁ Viscosity and light scattering validation kits
⦁ ReadyCal kits
⦁ Deuterated polymers
⦁ Tailor-made polymers
High Resolution Columns
⦁ GPC/SEC columns for all organic
eluents and all aqueous eluents
⦁ For high and low molecular weight
synthetic and biopolymers
⦁ For micro GPC/SEC up to preparative
scale
⦁ HighSpeed columns for fast analysis
Instrumentation and Software
⦁ GPC/SEC systems and components
⦁ Light scattering detectors
⦁ Viscosity detectors
⦁ dn/dc instrumentation
⦁ WinGPC UniChrom software
⦁ PoroCheck software for inverse GPC/SEC
Contract analysis (including HT-GPC
and specialty solvents)
⦁ Contract analysis
⦁ Molar mass determination
⦁ Branching/structure information
⦁ Method development and transfer
⦁ Complete product deformulation
⦁ Consulting
GPC/SEC Academy and Support
⦁ Full services from installation to valida-
tion, operation, and repair
⦁ GPC/SEC and software training schools
⦁ GPC/SEC in-house training
⦁ User meetings
⦁ NetCommunity with application and pub-
lication downloads
Facility PSS US office is located in Amherst, MA.
Headquarters are located in Mainz, Germany.
PSS also has offices in The Netherlands and
works with a network of distributor partners
in most other countries around the world.
PSS Polymer Standards Service Inc.
Company Description GPC/SEC/GFC is a versatile technique for characterizing polymers,
biopolymers, polysaccharides, and proteins used to determine
molecular weight, size, and architecture. PSS is fully dedicated to
the advancement of macromolecular liquid chromatography by
developing true solutions and providing competent and personal
support. Based on excellent products and cutting-edge technol-
ogy, PSS creates easy-to-use and powerful solutions for QC and
R&D. From a single molar mass reference material, contract
analysis, and consulting, to turn-key systems for GPC/SEC multi-
detection with light scattering, viscometry, mass spectrometry, or
fully compliant GPC/SEC for the pharmaceutical industry: PSS of-
fers products and services required for successful macromolecular
analysis with expert support by GPC/SEC enthusiasts.
Chief Chromatographic Techniques Supported ⦁ Size-exclusion chromatography (SEC)
⦁ Gel permeation chromatography (GPC)
⦁ Gel filtration chromatography (GFC)
⦁ High-Temperature GPC (HT-GPC)
⦁ GPC/SEC viscometry / light scattering / triple
⦁ GPC/SEC mass spectrometry
⦁ Two-dimensional chromatography
Markets Served Fully featured PSS applications provide solutions for the
analysis of natural and synthetic macromolecules divided into
the following sections:
⦁ Biopharma
⦁ Chemical manufacturing
⦁ Food analysis
PSS Polymer Standards
Service Inc.
160 Old Farm Road,
Amherst, MA 01002
T E L E P H O N E
(413) 835-0265
E - M A I L
W E B S I T E
www.pss-polymer.com
Y E A R F O U N D E D
1994
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 35www.chromatographyonline.com
Molex
18019 N 25th Ave.,
Phoenix, AZ 85023
T E L E P H O N E
(602) 375-4100
F A X
(630) 813-9995
W E B S I T E
www.molex.com/polymicro
Molex cal sciences, in addition to pharmaceutical,
environmental, and petrochemical analysis,
genomics, proteomics, neurology, clinical in-
strumentation, and a wide variety of related
technologies. Typical applications include re-
search, process monitoring and control, qual-
ity monitoring and control, forensics, package
leak testing, drug delivery, and DNA fragment
analysis (HID), in addition to unique applica-
tions requiring precision drawn capillary.
Major Products/Services Advances in chromatography research and
analysis have been aided by proprietary
improvements to the properties of Molex
synthetic fused capillary tubing and
techniques for handling, cutting, cleaving,
and laser machining. Molex manufactures
Polymicro high-strength, high-temperature,
flexible fused silica capillary tubing; square
capillary tubing; windowed capillary tubes;
UV transparent coating; precision cleaved-
to-length tubing; multi-lumen tubing;
multimode, step-index fused silica optical
fiber with polyimide, silicone, acrylate and
fluoropolymer buffers; solarization resistant
optical fiber for deep UV; broad spectrum
fiber; IR waveguides; fiber optic cables and
assemblies; silica and quartz base materials;
and micro-components including laser
machined fiber tips, telecommunications
ferrules and sleeves and Inner Lok™ unions
and Y-splitters for GC. New innovations
include VS Tight-Tolerance capillary, nano-
Capillary, and fiber bundles.
Facility Polymicro products are manufactured at
Molex’s facility located in Phoenix, Arizona.
The state-of-the art facility is ISO 9001,
13485, and 14001 certified and features
a glass laboratory, multiple draw towers,
sophisticated testing and laser machining
capabilities. We offer large and custom
orders, in addition to select, pre-packaged
Polymicro capillary tubing and optical fibers
for smaller orders. Please contact us at
[email protected] to initiate a
quote or request a copy of the Polymicro
solution handbook.
Company Description As a global leader in capillary tubing and specialty optical fiber
products for chromatography, Molex designs and manufactures
a large portion of Polymicro capillary tubing and multimode
step-index fibers used throughout the world. The company’s
extensive portfolio of products serves applications such as
aerospace, military and defense, analytical, astronomy, automo-
tive, biotechnology, communications, energy, manufacturing,
medical, pharmaceutical, and telecommunications.
Molex solves complex optical challenges for the world’s innova-
tors by anticipating and delivering what our customers need most.
From a globally integrated approach to seamless collaboration, we
bring expertise and innovation together to help bring your product
vision to reality. Our team works closely with yours to deliver the
results for your specific application needs by leveraging our heri-
tage of innovation, industry expertise and superior quality.
Chief Chromatographic Techniques Supported ⦁ Capillary Electrophoresis, CE
⦁ Cytometry
⦁ Gas Chromatography, GC
⦁ High Pressure Liquid Chromatography, HPLC
⦁ Lab-on-a-chip
⦁ Liquid Chromatography, LC
⦁ nano-Liquid Chromatography, nano-LC
⦁ Spectroscopy, Ultra Violet to Mid-Range Infrared
Markets ServedMolex capillary tubing, optical fibers, fiber optic assemblies, fiber
and tubing arrays, and bundles are commonly used in the analyti-
36 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Facilities EMEAI
Postnova Analytics GmbH
Max-Planck-Strasse 14
86899 Landsberg, Germany
Tel: +49 8191 985 688-0
Fax: +49 8191 985 688-99
UNITED KINGDOM
Postnova Analytics UK Ltd.
Unit 64, Malvern Hills Science Park,
Malvern, Worcestershire
WR14 3SZ, UK
Tel: +44 1684 585167
NORTH AMERICA
Postnova Analytics Inc.
230 South, 500 East, Suite 110
Salt Lake City, UT 84102, USA
Tel: +1 801 521-2004
Fax: +1 801 521-2884
Postnova Analytics Inc.
Company Description Postnova Analytics offers the worldwide unique FFF-Platform,
which is a range of different field-flow fractionation (FFF)
and light scattering systems (MALS) for advanced separation,
fractionation, and characterization of nanoparticles, proteins,
polymers, and bio-macromolecules. Postnova offers a truly
complete FFF-MALS product range, which is widely used for
applications in biopharmaceutical, nanotechnology, environ-
mental, food, cosmetics, and polymer science. The Postnova
FFF-Platform can also be ideally coupled to dynamic light scat-
tering (Malvern Zetasizer Nano DLS) and inductively coupled
plasma–mass spectrometry (Agilent Technologies ICP-MS),
which provides high resolution size and molar mass separa-
tions as well as characterization and element speciation.
Chief Chromatographic Techniques Supported⦁ Field-flow fractionation (FFF)
⦁ Asymmetric FFF
⦁ Thermal FFF
⦁ Centrifugal FFF
⦁ Multi-angle light scattering (MALS)
Postnova Analytics Inc.
230 South, 500 East, Suite 110
Salt Lake City, UT 84102
T E L E P H O N E
+1 (801) 521-2004
F A X
+1 (801) 521-2884
E - M A I L
W E B S I T E
www.postnova.com
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 37www.chromatographyonline.com
Major Products/Services Proton OnSite offers hydrogen, nitrogen,
and zero air gas generators to fit the
needs of any laboratory. These units are
suited to provide ultra-high purity gas with
consistent and predictable results. Also,
Proton OnSite is the only provider of “Lab
Server” solutions, allowing large labs to
supply numerous mass spec instruments
from a single, central generator. Our line
of on-site gas generators is a simpler, less
expensive, and less complex approach to
supplying superior gas for laboratory and
scientific applications. These generators
are compact in size and available in
stackable configurations to maximize your
available lab space.
Proton OnSite
Company Description Proton OnSite, a wholly owned subsidiary of Nel ASA, Oslo,
Norway (OSE:NEL) manufactures hydrogen, nitrogen, and
zero air generators that you can rely on. Our advanced proton
exchange membrane (PEM) electrolysis systems, coupled
with uncompromising attention to excellence and quality,
enable us to deliver, install, and support gas generation
on every continent. No other company has as large of a
commercial fleet or as long of a history with reliability, as
Proton OnSite. Exclusively focused on customer solutions, we
have the experience and products to ensure success.
Markets Served ⦁ Environmental sciences
⦁ Food and beverage industries
⦁ Life sciences
⦁ Petrochemical industry
⦁ Pharmaceutical industry
Proton OnSite
10 Technology Dr.
Wallingford, CT 06492
T E L E P H O N E
(203) 678-2300
F A X
(203) 949-8016
E - M A I L
W E B S I T E
www.protononsite.com
N U M B E R O F E M P L O Y E E S
100
Y E A R F O U N D E D
1996
38 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Major Products/Services GC, GC–MS, LC, LC–MS, Sample Prepara-
tion, Sample Introduction, SIFT-MS, Min-
iaturized Mass Detector, Trace Elemental
Analysis, Molecular Spectroscopy, NMR
Spectroscopy, Metrology, Digital Holo-
graphic Microscopy, Gas Generators
Facility Quantum Analytics is headquartered in
Foster City, California and has a Tech Cen-
ter in North Houston, Texas.
Quantum Analytics
Company Description Quantum Analytics is a value-added distributor for analytical
instrumentation across the United States. We offer technical
application services, a range of laboratory analytical instruments,
and flexible financing programs. We make it easier for customers
to get the lab equipment they need through flexible financing
solutions and customized technical support. We maintain a
multi-million dollar inventory of new and refurbished instruments
available for immediate delivery. Our technical services
include cross-platform system integration, installation, training,
application development and product support.
Chief Chromatographic Techniques SupportedGas chromatography, liquid chromatography, ion chromatog-
raphy, mass spectrometry, selected ion flow tube mass spec-
trometry, real-time gas analysis, miniature mass spectrometry
Markets Served Academia, automotive, biopharma, cannabis, chemicals, clini-
cal, defense, electronics, energy, environmental, food and
flavors, forensics and drugs, government, industrial, MEMS,
petrochemical, polymers, semiconductor, and more.
Quantum Analytics
3400 East Third Avenue,
Foster City, CA 94404
T E L E P H O N E
(650) 312-0900
F A X
(650) 312-0303
E - M A I L
W E B S I T E
www.LQA.com
Y E A R F O U N D E D
1989
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 39www.chromatographyonline.com
⦁ Industrial hygiene
⦁ Petrochemical
⦁ Pharmaceutical
Major Products/Services Plus 1 Service in everything we do. Living this
corporate core value every day ensures we
will surpass your expectations every time you
contact us! Our customer service team will
suggest time- and money-saving options and
is dedicated to getting your products to you
fast. Our technical service chemists can help
you from set-up to method development.
Visit our website where you can blog with
our chemists and review an extensive library
of technical publications, chromatograms,
product documentation, step-by-step guides,
interactive calculators, animations, and
educational material.
Restek’s commitment to continuous
innovation in chromatography sets us apart
from our competitors. We introduce and
stock hundreds of new products every year,
designed by chromatographers for
chromatographers.
⦁ Exceptional columns for UHPLC, HPLC,
LC–MS, GC, GC–MS, and GC×GC
⦁ Innovative accessories, instrument
replacement parts, and consumables
⦁ Air monitoring canisters and sampling
supplies
⦁ Sample prep products
⦁ Reference standards: stock and
custom-prepared formulations
⦁ Thousands of innovative products,
hundreds of chromatograms
Facilities Restek opened for business in 1985 in
a small business incubator in central
Pennsylvania. Today, more than 350
employee-owners work, play, and
celebrate milestones in a state-of-the-art
140,000-square-foot facility in Pennsylvania,
in our research facility in California, and in
our subsidiary locations in China, England,
France, Germany, Italy, and Japan.
Restek Corporation
Company DescriptionFor over 30 years, Restek has been a leader in developing
technologies and manufacturing products for gas and liquid
chromatography (GC and LC), including columns, reference
standards, sample preparation materials, accessories, and
more. We have decades of hands-on, practical experience
in chemistry, chromatography, and engineering, and our
reputation for going the extra mile with Plus 1 customer
service and top-performing products is well known
throughout the chromatography community. Restek is proud
to assist analysts around the world with monitoring the
quality and safety of air, water, soil, food, pharmaceuticals,
and petroleum. We proactively offer integrated solutions—
products, applications, and assistance—perfectly matched to
your needs, regardless of your industry. www.restek.com
Chief Chromatographic Techniques Supported ⦁ UHPLC
⦁ HPLC
⦁ LC–MS
⦁ GC
⦁ GC–MS
⦁ GC×GC
Markets Served ⦁ Air monitoring
⦁ Chemical
⦁ Clinical
⦁ Environmental
⦁ Food safety
⦁ Forensic
Restek Corporation
110 Benner Circle
Bellefonte, PA 16823
T E L E P H O N E
(800) 356-1688
F A X
(814) 353-1309
E - M A I L
W E B S I T E
www.restek.com
N U M B E R O F E M P L O Y E E S
350
Y E A R F O U N D E D
1985
40 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Sciencix
14261 W. Burnsville Pkwy.
Burnsville, MN 55306
T E L E P H O N E
(800) 682-6480
F A X
(952) 895-8493
E - M A I L
W E B S I T E
www.sciencix.com
N U M B E R O F E M P L O Y E E S
USA: 33
Y E A R F O U N D E D
1985
Sciencix
Company Description Sciencix has been a trusted provider of top brand HPLC
instrumentation products for more than 30 years. Our
products are equivalent to OEM products and can help reduce
repair expenses by 15% to 30%. Our mission is to serve our
customers by delivering quality service and quality products
at a reasonable price while exceeding expectations, every
moment.
Chief Chromatographic Techniques SupportedAny technique used in or related to high performance liquid
chromatography (HPLC) replacement parts.
Markets Served ⦁ Pharmaceutical
⦁ HPLC service companies
⦁ Academia
⦁ Biotechnology
⦁ Testing facilities
Major Products/Services Sciencix provides alternative repair parts to OEMs such as
Agilent®, Waters®, Shimadzu®, and many more. We offer
replacement parts for autosamplers, pumps, detector lamps,
filters, fittings, and tubing.
Facility Sciencix is headquartered in Burnsville, Minnesota. Our facility
includes manufacturing, R&D, and quality departments. We
are able to ship products world-wide.
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 41www.chromatographyonline.com
Facility Showa Denko America, Inc. based in New
York, New York provides technical and
sales support for Shodex HPLC columns
for customers and distributors in North
and South America. Our stock point in
New York allows for fast delivery in the
United States. Working with our laboratory
in Japan, we support customers’ analytical
needs.
TM
Shodex/Showa Denko America, Inc.
Company Description Shodex HPLC columns have been manufactured by Showa
Denko K.K. in Japan since 1973. Showa Denko K.K. is one of
Japan’s largest chemical companies. Our strength in polymer
synthesis led to the production of many unique and durable
polymer-based columns. The golden-mechanical fish is a
symbol of fast and smooth liquid chromatography analysis.
Chief Chromatographic Techniques SupportedHPLC column selection, method development, and trouble
shooting, as well as providing educational seminars
Markets Served ⦁ North America
⦁ South America
⦁ HPLC
⦁ Size exclusion
Major Products/Services Shodex carries thousands of different HPLC columns with
various separation modes. We are best known for high quality
size exclusion and saccharide analysis columns, as well as
unique polymer-based reversed and HILIC phase columns.
With additional selections of normal phase, ion exchange, ion
chromatography, high temperature, and multimode columns,
we are confident we can provide the most suitable column
for customers’ needs.
Shodex/Showa Denko
America, Inc.
420 Lexington Ave.
Suite #2335A
New York, NY 10170
T E L E P H O N E
(212) 370-0033
F A X
(212) 370-4566
E - M A I L
W E B S I T E
www.shodexhplc.com
Y E A R F O U N D E D
1973
42 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Shimadzu Scientific
Instruments
7102 Riverwood Drive
Columbia, MD 21046
T E L E P H O N E
(800) 477-1227
(410) 381-1227
F A X
(410) 381-1222
E - M A I L
W E B S I T E
www.ssi.shimadzu.com
N U M B E R O F E M P L O Y E E S
US: 440
Worldwide: 11,500
Y E A R F O U N D E D
Shimadzu Scientific
Instruments: 1975
Shimadzu Corporation: 1875
Shimadzu Scientific Instruments
Major Products/Services Shimadzu offers a wide range of advanced
UHPLC/HPLC, GC, and mass spectrometry
systems and components. Key systems
include:
⦁ Nexera X2 UHPLC—At 19,000 psi, the
modular Nexera achieves world-leading
sensitivity and superior separation
across the entire HPLC/UHPLC range.
⦁ Prominence UFLC/HPLC—Raises total
system productivity to new levels by
generating high-quality, reproducible
data without sacrificing performance,
reliability, or ruggedness.
⦁ i-Series Plus Integrated HPLC/UHPLC—
Providing the ultimate balance of high-
level performance and ease-of-use in
an integrated HPLC.
⦁ Nexera UC SFE-SFC—First fully auto-
mated system that combines on-line
SFE and SFC in a single flow path
⦁ LCMS-2020/8040/8045/8050/8060—
User-friendly single-quad and triple quad
LC–MS-MS systems deliver outstanding
speed, sensitivity, and reliability.
⦁ Nexis GC-2030—Offers a modern ap-
proach to a classic chromatographic
technique, delivering exceptional perfor-
mance and faster ROI.
⦁ System GC—Fully applicated GC systems
based on industry-standard methods.
⦁ GCMS-QP2020/SE/TQ8040/TQ8050—
With Smart Technologies, single-quad
and triple-quad GC–MS/MS systems
enable new possibilities in sensitivity,
durability, performance, stability, and
reliability.
Facilities Shimadzu’s US headquarters includes a cus-
tomer service and training center, a solution
center to showcase technologies, and an
innovation center for promoting collaborative
projects with customers. Shimadzu’s regional
facilities, strategically located around the US,
provide customers with local sales, service,
and technical support.
Company Description Shimadzu Scientific Instruments (SSI) is the North American
subsidiary of Shimadzu Corp., headquartered in Kyoto, Japan.
SSI was established in 1975 to provide analytical solutions
to a wide range of laboratories in the Americas. With a
vast installed base and preferred vendor status at many
institutions, SSI’s instruments are used by top researchers
across the globe, customers who can count on the stability,
experience, and support only Shimadzu offers.
Chief Chromatographic Techniques Supported ⦁ Ultra high-pressure LC
⦁ Ultra-fast LC
⦁ Analytical/prep HPLC
⦁ 2D LC
⦁ Online SFE-SFC
⦁ LC–MS/MS
⦁ GC
⦁ Comprehensive LC and GC
⦁ GC–MS/MS
Markets Served Shimadzu’s product line flexibility enables chromatographers
in any environment to select the instrument best suited to their
application. Shimadzu instruments are found in a wide range of
labs, including academic research, pharmaceutical, forensic, food
and beverages, petrochemical, life sciences, and environmental.
Shimadzu provides free technical support for the life of the
instruments and encourages customer alliances to further
product development.
Order consumables and accessories on-line at http://store.shimadzu.com Shimadzu Scientific Instruments Inc., 7102 Riverwood Dr., Columbia, MD 21046, USA
The Next Industry StandardGas Chromatograph
Nexis GC-2030, Shimadzu’s premier gas chromatograph, offers a modern approach to a classic
chromatographic technique. Designed with the user in mind, new innovative features, exceptional
performance and high-throughput capabilities will elevate your lab to the next level.
X Faster ROI with versatile, expandable configurations
X Reduced operating costs with Eco mode
X Improved workflow with touch-panel interface and remote communications
X Increased productivity with tool-free column installation and inlet maintenance
X Simplified operation with enhanced data browser and Quick-Batch function
Learn more about Shimadzu’s GC-2030. Visit us online at www.ssi.shimadzu.com/GC2030
44 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
⦁ Process
⦁ Analytical
⦁ Bio/Pharma
⦁ Oil & Gas
⦁ Refining
⦁ Petrochemical
⦁ Semiconductor
⦁ Corrosion
⦁ Coking/Fouling
⦁ Automotive
⦁ Aerospace
Major Products/Services SilcoTek® offers custom silicon coatings
to OEM and labs worldwide. Send in your
stainless steel, alloy, glass, or ceramic part
and we’ll apply our coatings in 10 days or
less. Need a coated part sooner? Many GC
and LC component suppliers offer coated
products from stock. Our coatings:
⦁ SilcoNert®: The most inert coating
available.
⦁ Dursan®: An inert, high durability
coating. Ideal for lab and field analysis.
⦁ Silcolloy®: A high purity corrosion
resistant coating.
⦁ SilcoKlean®: Prevent carbon coking and
fouling in automotive and aerospace.
⦁ SilcoGuard®: A low outgassing, high
purity coating, ideal for research and
semiconductor applications.
Facility SilcoTek® applies coatings to customer-
supplied products from their 35,000
square foot state-of-the-art facility located
in Bellefonte, Pennsylvania. Want to speak
to an expert about your application? Email
[email protected] or call + (814)
353-1778. To find a sales office near you,
please visit
www.silcotek.com/international-reps.
SilcoTek® Corporation
Company Description SilcoTek® Corporation is the world’s leading provider of flow
path deactivations and coatings. Our patented CVD coating
technologies improve analytical reliability, increase system
uptime, and boost your bottom line. SilcoTek’s coatings are used
in a large, diverse variety of industries and applications where
analytical accuracy and superior performance are critical. Whether
for industry-leading chemical compatibility, corrosion resistance,
bio-inertness, or hydrophobicity, SilcoTek coatings will expand the
material limits of your instruments and products.
Chief Chromatographic Techniques Supported⦁ Sulfur analysis
⦁ VOC analysis
⦁ Environmental
⦁ Thermal desorption
⦁ Fenceline monitoring
⦁ CEMS
⦁ Flare subpart JA
⦁ Stack monitoring
⦁ Protein analysis
⦁ NOx, SOx testing
⦁ GC
⦁ LC
Markets Served SilcoTek® coating technologies are utilized in diverse applications
and industry segments world wide. Since 1987, customers have
relied on SilcoTek® to improve the performance of their products
and increase revenue in the following markets and applications:
SilcoTek Corporation
225 PennTech Drive,
Bellefonte, PA 16823
T E L E P H O N E
(814) 353-1778
F A X
(814) 353-1697
E - M A I L
W E B S I T E
www.SilcoTek.com
N U M B E R O F E M P L O Y E E S
www.SilcoTek.com
Y E A R F O U N D E D
2009
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 45www.chromatographyonline.com
Sorbent Technologies, Inc.
5955 Peachtree Corners East
Norcross, GA 30071
T E L E P H O N E
(770) 936-0323
Toll-free (866) 767-2832
F A X
(770) 936-0326
E - M A I L
www.sorbtech.com
Y E A R F O U N D E D
2000
Sorbent Technologies, Inc. Markets Served Sorbtech has deep expertise in
chromatography and purification
research, and develops lasting, strong
relationships with the laboratories we
serve. Our broad product offering ensures
success—from simple lab procedures to
complex chromatographic techniques. The
industries we supply include academic,
bioprocess, chemical, food/beverage,
forensic, pharmaceutical, power, and
water. We assist from R&D method
development to process manufacturing:
CROs, academia (organic, bio-chemistry,
bioprocess, polymer/material science),
and government facilities (US FDA, NIH,
USDA, USGS, DOE).
Major Products/Services Sorbtech is one of the premier
chromatography suppliers, offering the
products, information and consultation you
need to meet lab and project requirements.
We provide comprehensive selection of
unique goods and services that deliver
value, including a free applications
database, high-end life science products,
and a customer referral program. To ease
the sales process, a personal product
specialist is assigned to each lab to
assist with method development and
optimization. This helps shift the burden of
purchasing supplies from your shoulders
to Sorbtech’s, allowing your lab to focus on
what matters most—achieving innovative,
successful outcomes.
Facility Sorbtech is located in Norcross, Georgia
within the Innovation Crescent—a
geographic area of economic development
entities dedicated to Georgia’s life science
and technology growth. Our facility includes
a state-of-the-art research and development
lab, production areas, and an efficient
warehouse that stores our vast inventory of
chromatography and purification products.
Company Description Sorbent Technologies, Inc. understands that chromatography
is the catalyst that drives success after weeks, or even years
of hard work. We partner with our customers to provide
the most effective solutions for their projects’ unique
challenges, helping meet stringent lab deadlines and budget
requirements, while protecting research integrity. In addition
to a large selection of chromatography products, Sorbtech
offers user-friendly laboratory equipment and supplies to
optimize performance, ensure lab safety, and create efficiency.
We select products based on performance and reliability
to help grant or investment dollars go further, delivering
confidence for lab, pilot, and industrial process applications.
Chief Chromatographic Techniques Supported⦁ Column chromatography (Flash, MPLC, and Gravity): bulk
media, cartridges, flash systems for laboratory and Process
Analytical Technology (PAT)
⦁ Size-Exclusion Chromatography (SEC): media and columns
for process purification of proteins and nucleic acids
⦁ Preparative Chromatography (FPLC): columns and media
for laboratory, pilot, and process
⦁ Thin-Layer Chromatography (TLC): plates, adsorbents, TLC
plate cutter, accessories
⦁ High-Pressure Liquid Chromatography (HPLC/UPLC): col-
umns and adsorbents for LC–MS, analytical, semi-prep, prep
⦁ Solid-Phase Extraction (SPE): cartridges and 96 well plates
⦁ Gas Chromatography: capillary columns with low bleeding
and ultra-low bleeding
⦁ Sample Preparation: SPE cartridges and syringe filters
46 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Major Products/Services Tosoh Bioscience offers a comprehensive
line of TSKgel prepacked columns for each of
the major chromatography modes, process
development tools, as well as TOYOPEARL,
TSKgel, and Ca++Pure-HA bulk resins for
laboratory scale purifications and process
chromatography.
Prepacked columns containing TSKgel
media are designed for the analysis of
proteins, peptides, other biopolymers, low
molecular weight compounds, and organic
soluble polymers. The packings in the
columns are either silica-based or polymer-
based material, in particle sizes ranging from
2 μm to 30 μm. Columns are available in
analytical through semi-preparative sizes, in
stainless steel, PEEK®, or glass.
A variety of screening tools composed of
TOYOPEARL and TSKgel process media are
available as an additional tool for convenient
scale-up. In addition, bulk media volumes of
<1 L are offered for process development.
The EcoSEC GPC System and the
EcoSEC High Temperature GPC System are
dedicated GPC instruments developed spe-
cifically for polymer analysis. These all-in-one
systems deliver top performance, reliability,
and superior results.
Facilities Tosoh Bioscience LLC is located in King of
Prussia, Pennsylvania. Supply chain offices
are in Grove City, Ohio. European operations
are in Griesheim, Germany. Asia is served by
Tosoh Corporation in Tokyo, Japan; Shang-
hai, China; and Singapore. Products are man-
ufactured by Tosoh Corporation in Japan.
Tosoh Bioscience LLC
Company Description Tosoh Bioscience LLC is a major supplier of chromatography
products worldwide, particularly to the pharmaceutical, bio-
technology, and polymer industries. The company is a division
of Tosoh Corporation, a global chemical company with head-
quarters and manufacturing facilities in Japan. Tosoh’s portfolio
of over 500 TSKgel® and TOYOPEARL® products encompasses
all common modes of liquid chromatography. We manufacture
analytical HPLC columns and provide bulk chromatographic res-
ins for the purification of biopharmaceutical drugs in commercial
manufacturing processes. Ca++Pure-HA™, a hydroxyapatite resin
for biomolecule purification, is a unique resin from Tosoh in that
it is both the ligand and base bead. Tosoh Bioscience LLC offers
dedicated systems for polymer characterization, the EcoSEC GPC
System and the EcoSEC High Temperature GPC System.
Chief Chromatographic Techniques Supported ⦁ Size exclusion
⦁ Reversed phase, normal phase (hydrophilic interaction)
⦁ Ion exchange
⦁ Hydrophobic interaction
⦁ Affinity
⦁ Mixed-mode
Markets Served Located in King of Prussia, Pennsylvania, Tosoh Bioscience
LLC provides sales and service to pharmaceutical and
biotechnology customers in North and South America.
Tosoh Bioscience, LLC
3604 Horizon Drive
Suite 100
King of Prussia, PA 19406
T E L E P H O N E
(484) 805-1219
(800) 366-4875
F A X
(610) 272-3028
E - M A I L
W E B S I T E
www.tosohbioscience.com
N U M B E R O F E M P L O Y E E S
35
Y E A R F O U N D E D
1987
DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 47www.chromatographyonline.com
Markets Served ⦁ Clinical
⦁ Environmental
⦁ Food safety
⦁ Forensic
⦁ Pharmaceutical
⦁ Veterinary
Major Products/Services ⦁ CLEAN SCREEN® SPE columns
⦁ CLEAN SCREEN FASt® columns
⦁ CLEAN SCREEN XCEL® SPE columns
⦁ CLEAN UP® SPE columns
⦁ STYRE SCREEN® SPE columns
⦁ XtrackT® SPE columns
⦁ ENVIRO-CLEAN® SPE cartridges
⦁ ENVIRO-CLEAN® universal cartridges
⦁ Chlorofiltr® Sorbents
⦁ QuEChERS
⦁ Quick QuEChERS
⦁ SpinFiltr™
⦁ Ultra Flash®
⦁ SELECTRA® HPLC columns
⦁ SELECTRASORB bulk sorbents
⦁ SELECTRA-SIL® derivatizing reagents
⦁ SELECTRAZYME® β-glucuronidase
⦁ ABALONASE™ β-glucuronidase
⦁ SELECT pH buffer pouches
⦁ GC liners
⦁ Positive pressure manifolds
⦁ Vacuum manifolds
Facility UCT is headquartered in Bristol,
Pennsylvania, with a research facility in
Scranton, Pennsylvania. Manufacturing
and distribution sites are in Lewistown,
Pennsylvania and Wexford, Ireland. UCT
is represented worldwide by over 47
partners and distributors.
UCT, Inc.
Company Description UCT is always at the forefront of sample preparation technology
and a leader in chromatography consumables. The company’s
wide range of highly reproducible silica and polymeric SPE
sorbents provide scientists a consistent extraction technique.
UCT is in a unique position within the sample prep and
chromatographic sorbent industry; we are one of the few
manufacturers of chromatographic silane materials. This expertise
provides us with greater control and extensive knowledge of the
chemical processes involved in producing high quality bonded
phases. UCT’s commitment to ensuring customer satisfaction
is accomplished by delivering on our promises: top quality,
reproducible chromatographic and sample prep products, and
unmatched technical support. The company’s sample prep
product lines include SPE cartridges and well plates, flash
chromatography columns, bulk sorbents, QuEChERS tubes,
derivatizing reagents, hydrolyzing enzymes, premeasured buffer
salts, and extraction manifolds; chromatography products include
HPLC columns and GC liners. All products are designed to meet
laboratory’s sample prep and chromatography needs.
Chief Chromatographic Techniques Supported ⦁ Solid-phase extraction
⦁ QuEChERS
⦁ Flash Chromatography
⦁ HPLC
UCT, Inc.
2731 Bartram Road
Bristol, PA 19007
T E L E P H O N E
(215) 781-9255
F A X
(215) 785-1226
E - M A I L
W E B S I T E
www.unitedchem.com
N U M B E R O F E M P L O Y E E S
>100
Y E A R F O U N D E D
1986
48 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Valco Instruments Co., Inc.
(VICI)
P.O. Box 55603
Houston, TX 77255
T E L E P H O N E
(800) 367-8424
F A X
(713) 688-8106
E - M A I L
W E B S I T E
www.vici.com
N U M B E R O F E M P L O Y E E S
US: 365
Europe: 135
Y E A R F O U N D E D
1968
Valco Instruments Co., Inc. (VICI)
GC capillary columns, and Dynacal gas
calibration standards. VICI Precision
Sampling manufactures analytical quality
syringes, sampling probes, as well as
standard and custom formed stainless and
special alloy tubing. VICI AG International
produces some Valco products as well as
the Jour line of HPLC fittings and lab safety
products. VICI DBS builds our new line of
laboratory hydrogen, nitrogen and zero air
gas generators.
Our newest products include the C82 line
of UHPLC valves, fittings that permit direct
connection of 360 μm tubing, the Universal
valve actuator, and the C52 platform
integrated HPLC injectors and selectors.
Facility With over 150 state-of-the-art CNC
machines, our instruments and
components are manufactured to exacting
standards at our facilities in Houston,
USA and Schenkon, Switzerland. Our
other manufacturing facilities include VICI
Metronics in Washington, VICI Precision
Sampling in Louisiana and VICI DBS
located in Italy. VICI Canada serves our
neighbor to the north from its offices in
Brockville, Ontario.
Company Description Founded in 1968, VICI is an international group of
companies. Our products can be found in nearly every
national lab, military facility, and university research
department around the globe. For 50 years VICI has driven
the field of chromatography with valves, fittings, detectors
and instruments for precision analytical, biomedical, and
biocompatible applications. We also customize components
and complete systems to improve chromatographic analyses.
Chief Chromatographic Techniques Supported ⦁ High Pressure Liquid Chromatography
⦁ Liquid Chromatography
⦁ Gas Chromatography
⦁ Flow Injection Analysis
⦁ Supercritical Fluid Chromatography
⦁ Gas Permeation Chromatography
⦁ Process Gas Chromatography
Major Products/Services VICI produces a complete line of valves for analytical chemistry.
We specialize in zero dead volume fittings and filters for the
separation sciences and are the original creators of the pulse
discharge dectector (PDD). Our miniPDD consumes less than
one-fifth the amount of helium required by the PDDs used on
complete GC systems. It has similar sensitivity to the original
PDD with a slightly lower dynamic range.
Our VICI Metronics division produces PEEK, FEP, PFA, and
ETFE tubing, ambient temperature gas purifiers, ValcoBond
Everything you need for
Fast GC
VICI Fast Temperature Programmer• Four independently rampable temp zones up to 2500°C/min
VICI Heater Transfer Lines• Multiple size and shape options• Temperature sensor
VICI Columns• Available in any phase either as a fused silica, metal or packed column
VICI Fan Modules• High flow fan size options and voltage options• Multiple mounting options
No more cold or hot spots. Program all the elements simultaneously.
Visit www.vici.com/fast-gc for more information.
Valco Instruments Co. Inc.
Fast GC Product Options1. Individual components open stock
2. Kit for updating or building GC
3. Modular form configuration
4. Custom applied instruments
Fast GC Components
SimDis Analysis
50 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Major Products/Services WTC’s family of instruments includes:
⦁ DAWN HELEOS: An 18-angle SEC–
MALS detector that can be used at
ambient, elevated, and below ambient
temperatures for polymers, particles, and
biopolymers.
⦁ miniDAWN: A SEC–MALS detector ideal
for proteins and peptides and molar
mass materials below 1 million Da.
⦁ μDAWN: The world’s first MALS detector
for UHPLC. Compatible with all UHPLCs
for molar mass and size determinations.
⦁ UT-rEX: The world’s first refractive index
(RI) detector for UHPLC. Operates at the
same wavelength of light as the μDAWN.
⦁ Eclipse system: For separation of
macromolecules and nanoparticles in
solution.
⦁ Optilab TrEX: The most advanced RI
detector in the world with 256 times
the detection power and 50 times
the dynamic range of any other RI
detector. The TrEX can be operated
below or above ambient temperature
and determine dn/dc at the same
wavelength of light as the light-
scattering instrument.
⦁ WyattQELS: A quasi-elastic (dynamic)
light-scattering instrument that can be
interfaced to the DAWN HELEOS or the
miniDAWN TREOS to determine particle
sizes as small as 1 nm.
⦁ ViscoStar viscometer: A state-of-the-
art intrinsic viscosity detector that can
be operated above or below ambient
temperature with its precise Peltier
thermostatic controls.
⦁ WTC Protein Columns: A family of new
silica based columns specifically designed
for SEC–MALS protein applications. These
columns retain all the important features
of a first-class SEC column for protein
separations.
Facility WTC maintains more than 45,000 square
feet of modern electronics, optical
prototype, machine shop, and laboratory
facilities in Santa Barbara, California.
Wyatt Technology Corp.
Company Description Wyatt Technology, a 35-year old family-owned and operated
enterprise, is the world’s largest company dedicated to SEC-
MALS detectors for absolute macromolecular characterization.
Our instrumentation provides the essential techniques for
characterizing macromolecules and nanoparticles in solution, in
order to determine molar mass, size, charge, and interactions.
Wyatt’s extensive applications laboratories and its full-time staff
(including more than 18 PhD scientists) means our customers
receive deep technical expertise and support. To ensure the
personal touch, “Light Scattering University” (LSU) is included
with most of our instruments. We bring you to Santa Barbara
to de-mystify light scattering, work you hard but feed you well,
and, of course, explain how to get the most from your Wyatt
Technology equipment.
Chief Chromatographic Techniques Supported ⦁ UHPLC
⦁ GPC/SEC/GFC
⦁ RPC
⦁ Field-flow fractionation
Markets Served Wyatt’s products are the most widely used multi-angle light
scattering (MALS) detectors in the world. They are employed
in thousands of laboratories including, but not limited to,
companies involved in biotechnology, pharmaceutical
development, and chemical and petrochemical research, in
addition to government national labs and academic facilities.
Wyatt Technology Corp.
6330 Hollister Ave.,
Santa Barbara, CA 93117
T E L E P H O N E
(805) 681-9009
F A X
(805) 681-0123
E - M A I L
W E B S I T E
www.wyatt.com
N U M B E R O F E M P L O Y E E S
120
Y E A R F O U N D E D
1982
wyatt.com
© 2017 Wyatt Technology and Leo Cullum from cartoonbank.com. All rights reserved. All trademarks and registered trademarks are properties of their respective holders.
That’s the problem with relying on the assumptions of column calibration to characterize macromolecules.
You don’t really know if your standards and your samples are the same! Perhaps that’s why every major
pharmaceutical and biotechnology company, as well as most federal regulatory agencies are switching from
relative methods to Wyatt Technology’s absolute measurements. We’ll show you how to end your dependence
on reference standards, in order to make you and
your products a whole lot more convincing. See our
webinar on Absolute Biophysical Characterization
at wyatt.com/Absolute.
52 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
Markets Served Located in Allentown, Pennsylvania, YMC
America, Inc., provides sales and service to
pharmaceutical, biotechnology, biopharma-
ceutical, government, and environmental
customers in North and South America.
Major Products/Services ⦁ YMC-Triart
⦁ YMC-BioPro
⦁ Meteoric Core (Core-shell)
⦁ Chiral, CHIRAL ART: Amylose-C, Cellu-
lose-C, Amylose-SA, Cellulose-SB, Cellu-
lose-SC; Cellulose-SJ; YMC Chiral Series:
NEA (R), (S), α-CD BR, β-CD BR, γ-CD BR
⦁ Reversed-phase, ODS: YMC-Triart C18
and C18 ExRS, Meteoric Core C18 and
C18 BIO; Pro Series: Pro C18, Hydro-
sphere C18, Pro C18 RS; YMC-Pack
ODS Series: ODS-A, ODS-AM, ODS-AQ,
ODS-AL; J’sphere ODS: ODS-H80, ODS-
M80, ODS-L80; YMC-Pack PolymerC18
⦁ Other than ODS: YMC-Triart C8, Phenyl,
and PFP; Meteoric Core C8; Pro C8; Pro
C4; YMCbasic, YMC Carotenoid; YMC-
Pack Series: C8, C4, TMS, Ph (Phenyl),
CN (Cyano), Protein-RP, YMCbasic, YMC
Carotenoid
⦁ Normal phase: YMC-Triart Diol-HILIC;
YMC-Pack Series: SIL, SIL-06, Diol-NP,
CN, PVA-Sil, Polyamine II, NH2, PA-G
⦁ Ion-Exchange, YMC-BioPro Series:
QA (porous quaternary amine anion
exchange), SF (porous sulfopropyl cation
exchange), QA-F (non-porous QA), SP-F
(non-porous SF)
⦁ Size-Exclusion, YMC-Pack Series:
Diol-60, Diol-120, Diol-200, Diol-300;
YMC-GPC
In addition to all of YMC’s analytical chroma-
tography offerings, most chromatography
chemistries are scalable to bulk packing ma-
terials for lab-scale and production-scale puri-
fications. YMC also offers preparative systems
for discovery, pilot-scale, and production.
Facilities YMC America, Inc. – Allentown, Pennsylvania
YMC Co., Ltd. – Kyoto, Tokyo, Japan
YMC Europe GmbH – Dinslaken, Germany
YMC India Pvt. Ltd. – New Delhi, India
YMC China – Shanghai, China
YMC Korea Co., Ltd. – Gyeonggi-do, Korea
YMC Taiwan Co., Ltd. – Taipei City, Taiwan
YMC Singapore Tradelinks Pte, Ltd. –
Singapore
YMC Schweiz GmbH – Basel, Switzerland
YMC America, Inc.
Company Description YMC America, Inc., is the exclusive sales and support channel for
separations leader YMC Co., Ltd., whose products include station-
ary phase chemistries primarily used for high and low pressure
separation science. Stationary phases designed for use with
high performance liquid and supercritical fluid chromatography
techniques are available as stocked standard items and custom
products are available for specialized application needs. Products
bonded to spherical silica, hybrid silica, and polymeric-based par-
ticles ranging in size from 1.9 to 150 μm are available in packed
columns or in bulk chemical packaging formats. YMC America
maintains offices, application support laboratories, and a ware-
house in Allentown, Pennsylvania and manages the applications
support and sub-distribution of YMC products throughout the
Western hemisphere, either through direct contact with its cus-
tomers or via a network of distributors throughout the Americas.
YMC products are available on a world-wide basis.
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ UHPLC
⦁ Supercritical fluid chromatography (SFC)
⦁ Chiral chromatography
⦁ Ion-exchange (biomolecules) chromatography
⦁ Size-exclusion chromatography (SEC)
⦁ Preparative chromatography
⦁ HILIC chromatography
⦁ Superficially porous particle (core-shell) chromatography
⦁ Nano–capillary chromatography
⦁ Preparative chromatography systems
⦁ Flow reactor chemistry
YMC America, Inc.
941 Marcon Blvd.
Suite 201
Allentown, PA 18109
T E L E P H O N E
(610) 266-8650
F A X
(610) 266-8652
E - M A I L
W E B S I T E
www.ymcamerica.com
N U M B E R O F E M P L O Y E E S
Worldwide: 350
Y E A R F O U N D E D
1980
YMC delivers reproducibility in over 30
distinctly different phase chemistries.
Beyond reproducibility, YMC delivers innovation … in hybrid phases,
core-shell technology, chiral separations, ion exchange, and more.
Scalable from UHPLC to the production floor.
Bring your separation challenges to YMC.
www.ymcamerica.com
+1-610-266-8650
54 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com
ZirChrom
Separations, Inc.
617 Pierce Street
Anoka, MN 55303
T E L E P H O N E
(763) 421-5264
F A X
(763) 421-2319
E - M A I L
W E B S I T E
www.zirchrom.com
Y E A R F O U N D E D
1995
ZirChrom Separations, Inc.
Markets Served Pharmaceutical, nutraceutical, basic sci-
ence research, antibody purification, food
and cosmetics, chemical manufacturing
(pesticides, polymers), biological research
(phosphopeptide enrichment), and chiral
separation.
Major Products/Services ZirChrom offers a full line of zirconia and
titania-based HPLC products for chromato-
graphic method development. ZirChrom
HPLC products offer unique selectivity, ex-
acting reproducibility, high efficiency, and
ultra-stability. All phases are available in
packed, prep, semi-prep, bulk, microbore,
and SPE formats:
Reversed Phase (Diamondbond®-C18,
ZirChrom®-CARB, ZirChrom®-EZ,
ZirChrom®-MS, ZirChrom®-PBD,
ZirChrom®-PS, Sachtopore®-RP);
Ion-Exchange (ZirChrom®-PEZ,
ZirChrom®-SAX, ZirChrom®-SHAX,
ZirChrom®-WAX, ZirChrom®-WCX);
Other/Specialty (ZirChrom®-PHASE,
Rhinophase®-AB, Sachtopore®-NP);
Chiral (ZirChrom®-Chiral(S)LEU,
ZirChrom®-Chiral(R)NESA, ZirChrom®
-Chiral(S)NESA, ZirChrom®-Chiral(S)PG,
ZirChrom®-Chiral(R)PG, ZirChrom®
-CelluloZe).
Facilities ZirChrom is headquartered in Anoka,
Minnesota, and has distributors
worldwide.
Company Description ZirChrom Separations, Inc. is a company that was formed
in 1995 and is located in Anoka, Minnesota. ZirChrom
manufactures a full line of zirconia and titania based high
performance chromatographic materials for the analytical
analysis of compounds primarily by high performance liquid
chromatography (HPLC).
Chief Chromatographic Techniques Supported ⦁ HPLC
⦁ Semi-prep chromatography
⦁ Prep chromatography
⦁ Ion exchange chromatography
⦁ Reversed phase chromatography
⦁ HILIC
⦁ Sample prep
⦁ Multi-modal chromatography
⦁ Pseudo-affinity chromatography
⦁ Chiral chromatography
⦁ Bulk materials
⦁ Microbore-HPLC
⦁ UHPLC/Sub-2 micron
APPLICATION NOTES – DECEMBER 2017 55
MEDICAL/BIOLOGICAL
Presented is a rapid, three step SPE procedure, that includes a
concentration step, for the identifi cation and quantifi cation of fentanyl
and its major urinary metabolite norfentanyl, along with 11 additional
synthetic opioid compounds: acryl fentanyl, desmethyl U-47700,
furanyl fentanyl, methoxyacetyl fentanyl, remifentanil, tetrahydrofuran
fentanyl, U-47700, U-50488, U-51754, W-15, and W-18.
Procedure
1. Sample Pretreatment – Hydrolysis
a) To 1 mL of urine sample add 1 mL of Abalonase™+ working
enzyme stock solution (10,000 Fishman units/mL).
b) Add internal standard(s).
c) Gently mix by inversion or vortex 10 to 15 s prior to use.
d) Hydrolyze for 15 min to one h from room temperature to 70 °C.
2. SPE Procedure
a) Apply the sample to the SPE cartridge (if required, use a low
vacuum to draw the sample through at ≤3 mL/min).
b) Wash Cartridge:
1. 1 × 2 mL D.I. H2O.
2. 1 × 2 mL 75:25 D.I. H2O: MeOH.
3. Dry column under full vacuum or pressure for 10 min.
c) Elution:
1. Elute with 1 × 2 mL CH2Cl
2/IPA/NH
4OH (75:20:5).
2. Evaporate the sample to dryness under a gentle stream
of nitrogen.
3. Reconstitute in 100 μL 95:5 D.I. H2O: MeOH and vortex
for 1 min.
4. Transfer sample to autosampler vial containing a low
volume insert.
Instrumental
LC–MS/MS: Shimadzu Nexera X2 LC-30AD coupled to a
Shimadzu LCMS-8050
Column: UCT Selectra® PFPP HPLC column
Analysis of Fentanyl
and Designer Fentanyl
Derivatives in Urine Using
SPE and HPLC–MS/MSDanielle Mackowsky, UCT, LLC
UCT, LLC2731 Bartram Rd, Bristol, PA 19007
Tel: (800) 385-3153
Website: www.unitedchem.com
Extraction/Analytical Materials
CSXCE106Clean Screen® XCEL I
130 mg / 6 mL SPE Cartridge
ASFBETA-GLUC
AbalonaseTM +
Purifi ed Beta-glucuronidase with enhanced
sulfatase activity
SLPFPP100ID21-3UMSelectra® PFPP HPLC column
100 × 2.1 mm, 3 μm
SLPFPPGDC20-3UMSelectra® C18 guard column
10 × 2.1 mm, 3 μm
100 × 2.1 mm, 3 μm
Guard column: UCT Selectra® PFPP guard
Column - 10 × 2.1 mm, 3 μm
Injection volume: 5 μL
Mobile phase A: D.I. H2O + 0.1% formic acid
Mobile phase B: MEOH + 0.1% formic acid
Column fl ow rate: 0.30 mL/min
Results
Recovery and RSD% from spiked coffee samples
Compound 1 ng/mL 5 ng/mL 15 ng/mL
Acryl Fentanyl 92 82 94
Desmethyl U-47700 95 89 82
Fentanyl 99 100 83
Furanyl Fentanyl 97 80 91
Methoxyacetyl Fentanyl 94 91 97
Norfentanyl 93 88 88
Remifentanil 90 92 91
Tetrahydrofuran Fentanyl 100 84 85
U-47700 94 85 94
U-50488 99 96 75
U-51754 96 93 83
W-15 82 98 95
W-18 82 98 90
Conclusion
A fast and effective method was developed for the determination of
fentanyl and other synthethic opiate compounds in urine samples. All
analytes of interest were extracted using a Clean Screen XCEL® I col-
umn. Analysis of the samples was performed by LC–MS/MS utilizing a
Selectra® PFPP HPLC column which allowed for improved separation
of furanyl fentanyl and fentanyl, when compared to other column phas-
es. Absolute recoveries ranged from 75–100% for all three control levels
tested. With the unfortunate (and often unaware) abuse of synthetic opi-
ates throughout the United States, it is critical that forensic laboratories
have accurate and rapid SPE methods for the identifi cation of this class
of compounds. The proceeding method will be of great use as drugs
with similar structures start to be found in future casework.
56 APPLICATION NOTES – DECEMBER 2017
MEDICAL/BIOLOGICAL
Methods for monitoring alcohol consumption biomarkers EtG
and EtS are generally limited by poor retention and coelution
with matrix interferences, as well as by long analysis times and
short column lifetimes. The dilute-and-shoot EtG/EtS LC–MS/MS
analysis developed here using the novel Raptor EtG/EtS column
easily resolves EtG and EtS from matrix interferences, provid-
ing consistent, accurate results for high-throughput labs testing
human urine samples for alcohol consumption.
Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are important bio-
markers of alcohol use. The detection of these metabolites offers
many advantages for abstinence monitoring, including a three-day
detection window, good stability in properly stored specimens, and
analytical specifi city. However, typical methods for EtG/EtS analysis
have several shortfalls: poor resolution of EtG and EtS from matrix
components, long run times that limit sample throughput, and short
column lifetimes.
In this study, a simple dilute-and-shoot method was developed,
validated, and applied to patient samples for EtG/EtS LC–MS/MS
analysis in human urine. The method was developed on a Raptor
EtG/EtS column because it provides the retention characteristics that
are needed to consistently elute the target analytes away from matrix
interferences. In addition, it is rugged and long-lasting, which is
benefi cial to high-throughput laboratories.
Experimental Conditions
Standard and Sample Preparation
Human urine (alcohol free) was fortifi ed with EtG and EtS in order to
prepare calibration standards and QC samples. The concentration of
calibration standards ranged from 50–5000 ng/mL for both analytes.
Four QC levels were prepared at 50, 150, 750, and 4000 ng/mL.
Aliquots (50 μL) of urine were diluted with 950 μL of the work-
ing internal standard (100 ng/mL EtG-d5 and 25 ng/mL EtS-d5 in
0.1% formic acid in water). Samples were vortexed at 3500 rpm for
10 s to mix followed by centrifugation at 3000 rpm for 5 min at 10 °C.
Additional double blanks were extracted for column equilibration.
Defi nitive EtG/EtS
LC–MS/MS Analysis: A Rugged
4-Min Method for High-
Throughput LaboratoriesJustin Steimling and Frances Carroll, Restek Corporation
Time (min)
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0
MatrixInterference
MatrixInterference
MatrixInterference
MatrixInterference
EtG
EtG
EtS
Injection 1
Injection 1,000
EtS
Figure 1: Rugged Raptor EtG/EtS columns provide reliable results for EtG/EtS LC–MS/MS analysis allowing more samples to be analyzed be-tween column changes.
Table I: Analyte transitions
Analyte Precursor Ion Product Ion Product Ion
EtG-d5 225.9 84.9 –
EtG 220.8 84.9 74.8
EtS-d5 129.7 97.7 –
EtS 124.7 96.8 79.7
LC–MS/MS Analysis
In order to ensure good response, peak shape, and retention time
consistency, the analytical column was conditioned prior to use with
30 matrix injections that were run through the full gradient program.
Instrument parameters for EtG/EtS LC–MS/MS analysis are shown
below and the analyte transitions are given in Table I.
Analytical column: Raptor EtG/EtS, 2.7 μm, 100 mm × 2.1 mm
(cat.# 9325A12)
Guard column: UltraShield UHPLC precolumn fi lter, 0.2 μm frit
(cat.# 25809)
Mobile phase A: 0.1% Formic acid in water
Mobile phase B: 0.1% Formic acid in acetonitrile
Gradient Time (min) %B
0.00 5
2.50 35
2.51 5
4.00 5
Flow rate: 0.5 mL/min
Injection volume: 10 μL
Column temp.: 35 °C
Ion mode: Negative ESI
Results
Chromatographic Performance
As shown in Figure 1, a fast, 4-min EtG/EtS LC–MS/MS analysis was
obtained from the direct (dilute-and-shoot) injection of supernatant.
Both EtG and EtS are clearly resolved from matrix interferences, mak-
ing accurate peak identifi cation an easy task. Even after 1000 sample
injections on a Raptor EtG/EtS column, all chromatographic peaks
APPLICATION NOTES – DECEMBER 2017 57
MEDICAL/BIOLOGICAL
Restek Corporation110 Benner Circle, Bellefonte, PA 16823
tel. (800) 356-1688, fax (814) 353-1309
Website: www.restek.com
Time (min)
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0
EtG
(overlay of 5,000 ng/mL standard)
Matrix double
blank injection with
post-column EtG
infusion.
Figure 2: EtG elutes outside the matrix suppression zones (double blank injection with post-column EtG infusion).
maintained the initial peak shape, retention time, and response.
Linearity
Using linear 1/x weighted regression for EtG and EtS, both compounds
showed good linearity with r2 values of 0.999 or greater over a calibra-
tion range of 50–5000 ng/mL. Accurate calibration down to 50 ng/mL
allows low-level positive results to be reported with confi dence.
Accuracy & Precision
Precision and accuracy analyses were performed on three different
days. The method accuracy was demonstrated to be within 6.3%
of the nominal concentration for all QC levels for both EtG and EtS.
The %RSD was 1.28–9.19% and 4.01–6.82% for intra- and inter-
run, respectively, at the QC LLOQ. The %RSD was 0.675–7.78%
and 1.00–4.99% for intra- and inter-run, respectively, at the QC low,
mid, and high levels, indicating good method precision for EtG/EtS
LC–MS/MS analysis (Table II).
Matrix Effect
Samples prepared in matrix show approximately 80% (EtG) and
100% (EtS) of the signal obtained when samples are prepared in sol-
vent across all QC levels, demonstrating that matrix effect is minimal.
The sensitivity and consistency of the EtG response at the LLOQ is
only possible because retention on the Raptor EtG/EtS column was
optimized so that EtG elutes outside the zones of matrix suppression
(Figure 2). Because the optimal EtG elution time was established here
during method development, clinical labs adopting the method can
be confi dent that matrix suppression has already been minimized.
This, in combination with the established calibration range and LLOQ,
demonstrates that the method provides excellent sensitivity for EtG,
which is generally harder to detect at low levels than EtS.
Sample Analysis
The robustness of the method was further tested by monitoring fi ve
patient samples with positive results for both EtG and EtS in the lower
end of the linear range across multiple instrument platforms and nine
Table II: Accuracy and precision of QC samples
QC LLOQ (50 ng/mL) QC Low (150 ng/mL)QC Mid
(750 ng/mL)
QC High
(4000 ng/mL)
Analyte
Average
Conc.
(ng/mL)
Average
Accuracy
(%)
%RSD
Average
Conc.
(ng/mL)
Average
Accuracy (%)%RSD
Average
Conc.
(ng/mL)
Average
Accuracy
(%)
%RSD
Average
Conc.
(ng/mL)
Average
Accuracy (%)%RSD
EtG 51.2 102 6.82 143 95.2 4.99 749 99.8 3.68 3949 98.7 2.04
EtS 46.9 93.7 4.01 143 95.6 1.42 762 102 1.00 3958 98.9 1.59
Table III: Inter-run precision of patient samples across
multiple instrument platforms and nine column lots
Patient
Sample
Average EtG
Concentration
(ng/mL)
%RSD
Average EtS
Concentration
(ng/mL)
%RSD
A 216 6.13 78.0 3.56
B 1167 4.81 300 3.24
C 98.2 9.76 82.4 4.01
D 319 8.33 233 3.63
E 247 11.2 163 8.70
lots of Raptor EtG/EtS columns. The precision of the results (n=9) was
found to range from 3.24–11.2% for both analytes over multiple days
and sample preparations, indicating excellent robustness and ease of
method transfer (Table III). High-throughput labs in particular will benefi t
from implementing this rugged EtG/EtS LC–MS/MS analysis method as
highly consistent performance is seen across a wide range of scenarios.
Conclusions
It was demonstrated that the Raptor EtG/EtS column is excellent for
the rapid and accurate analysis of EtG and EtS in human urine. Iso-
baric matrix interferences are easily resolved, preventing issues with
peak identifi cation and quantitation. In addition, superior sensitiv-
ity for EtG is achieved because it elutes outside the zones of matrix
suppression that are typically observed with dilute-and-shoot assays.
With a fast and simple sample preparation procedure and only 4 min
of chromatographic analysis time, the EtG/EtS LC–MS/MS method
established here provides accurate, high-throughput monitoring of
alcohol consumption. For the full article, visit www.restek.com and
enter “CFAN2736” in
the search box.
FOOD & BEVERAGE
58 APPLICATION NOTES – DECEMBER 2017
A sensitive determination method with automated solid-
phase extraction is presented and the recovery rates are
compared with manual extraction. The automated extraction
was developed with the SPE robot FREESTYLETM SPE module
(LCTech GmbH). According to the European Regulation (EC)
No 470/2009 CHROMABOND® HLB was applied for solid-phase
extraction (1). An HPLC–MS/MS method with the biphenyl
phase NUCLEODUR® π2 was used for the subsequent analysis.
Chloramphenicol is a broad-spectrum antibiotic against Gram-pos-
itive and Gram-negative bacteria. It has many side effects. Thus, it
is only used nowadays as reserve antibiotic (2). For warranty of the
food safety, many countries declare limits for pharmaceuticals in
animal products. The necessary determination methods demand
a high-performance solid-phase extraction to reliably detect and
quantify residues in trace level with the subsequent analysis.
Automated Solid-Phase Extraction (3)
Sample pretreatment: Weigh out 5 g of honey. Add 4 mL water and shake
rigorously for 30 s. Spike with 1 mL standard solution (chloramphenicol,
chloramphenicol-d5 , c = 5 ng/mL in methanol) and shake rigorously for
30 s. Add 15 mL ethyl acetate and shake rigorously for 30 s. Centrifuge
at 3000 rpm for 10 min. Take 12 mL of supernantant and evaporate
extracts to dryness at 40 °C under a stream of nitrogen. Redissolve
residue in 10 mL water.
Extraction:
SPE robot: FREESTYLETM SPE module, LCTech
SPE column: CHROMABOND® HLB, 3 mL, 200 mg, MACHEREY-
NAGEL REF 730924
Column conditioning: 3 mL methanol (dispensing speed 1 mL/min),
then 5 mL dest. water (dispensing speed 1 mL/min)
Sample application: 9 mL pretreated sample (dispensing speed
3 mL/min over sample loop)
Washing: 10 mL dest. water (dispensing speed 3 mL/min)
Drying: 100 mL air (dispensing speed 100 mL/min)
Elution: 5 mL ethyl acetate – methanol (80:20, v/v)
Drying: 100 mL air (dispensing speed 100 mL/min)
Evaporation: under nitrogen, 40 °C
Reconstitution: in 1 mL dest. water – acetonitrile (95:5, v/v)
Determination of Chloramphenicol in Honey with Automated
Solid-Phase Extraction and HPLC–MS/MS DetectionHans Rainer Wollseifen, Johannes Brand, and Detlef Lambrecht, MACHEREY-NAGEL GMBH & CO. KG
Figure 1: Chromatogram of spiked honey sample (1 μg/kg).
FOOD & BEVERAGE
APPLICATION NOTES – DECEMBER 2017 59
Manual Solid-Phase Extraction
After identical sample pretreatment as for automated solid-phase
extraction the sample was manually extracted by a vacuum manifold
under comparable SPE conditions.
Subsequent Analysis: HPLC–MS/MS (4)
HPLC column: EC 150/2 NUCLEODUR® π2, 5 μm, MACHEREY-
NAGEL REF 760624.20
Eluent A: dest. water
Eluent B: acetonitrile
Gradient: 5–95% B in 7.5 min, 95% B for 1 min, 95–5% B in
1 min, 5% B for 5 min
Flow rate: 0.30 mL/min
Temperature: 35 °C
Injection volume: 5 μL
MS/MS detection: API 5500 (AB Sciex), ion source ESI, negative ion-
ization mode, scan type MRM (chloramphenicol: [M-H]- 320.9, 152.0
(Q1), 256.0 (Q2); chloramphenicol-d5: [M-H]- 325.9, 156.6 (Q1),
261.8 (Q2)), curtain gas 35 psig, ion spray voltage -4500 V, temperature
450 °C, nebulizer gas 45 psig, turbo gas 45 psig, CAD medium
Results
Solid-phase extraction of chloramphenicol from the matrix honey
with CHROMABOND® HLB results in an outstanding recovery. The
recovery rates presented in Table I show that higher recovery rates
up to 90% with very good reproducibility (< 5%) are received by
automation with the SPE robot FREESTYLETM SPE module. A cor-
rection with an internal standard is especially advantageous for the
manual sample preparation. Figure 1 shows the chromatogram of
a sample extract from spiked honey. A defi nite identifi cation and
quantifi cation is possible due to a clear separation of matrix interfer-
ences from the analyte peak.
The liquid chromatographic method could be accelerated by us-
ing a shorter HPLC column due to good retention of chlorampheni-
col by the additional π-π-interactions of NUCLEODUR® π2.
Conclusion
A determination method for chloramphenicol in honey has been
developed, which receives very good results—especially by the
automation of solid-phase extraction.
References
(1) Regulation (EC) No 470/2009 of the European Parliament and of the Council.
(2) Side-effects of Chloramphenicol and Aureomycin, Br Med J., 384–2, 388–392
(1951).
(3) Application No. 306350, Macherey-Nagel, available from www.mn-net.
com/apps
(4) Application No. 128140, Macherey-Nagel, available from www.mn-net.
com/apps
MACHEREY-NAGEL GMBH & CO. KGNeumann-Neander-Str. 6–8, 52355 Düren, Germany
Tel. +49-(0)2421-969-0
Website: www.mn-net.com
Table I: Comparison of recovery rates from automated to manual solid-
phase extraction
(%) Recovery
rates
Chloramphenicol Chloramphenicol
(corrected by
int. standard)
Chloramphenicol-d5
Automated SPE 86.8 ± 4.4 (n = 4) 90.8 ± 4.6 (n = 4) 95.6 ± 4.5 (n = 4)
Manual SPE 74.6 ± 2.7 (n = 3) 92.9 ± 3.4 (n = 3) 80.3 ± 4.7 (n = 3)
FOOD & BEVERAGE
60 APPLICATION NOTES – DECEMBER 2017
Fumonisins are naturally occurring Mycotoxins produced by
Fusarium mold species. Fumonisins contamination happens
worldwide in many agricultural commodities, especially in corn,
and is usually associated with dry and hot weather followed by
periods of high humidity. Out of more than 10 types of Fumoni-
sins, Fumonisin FB1 is the most prevalent and toxic, followed by
Fumonisins FB2 and FB3. Fumonisins are classifi ed as possibly
carcinogenic for humans and also cause health problems in ani-
mals, especially in equids and swine. FDA sets total Fumonisins
limits in human foods between 2–4 μg/g and in animal feed be-
tween 5–100 μg/g.
Since Fumonisins don’t have a chromophore and don’t fluo-
resce, derivatization is needed to achieve the required sensitivi-
ty of detection. We developed a fast and sensitive HPLC method
with post-column derivatization that is capable of analyzing Fu-
monisins in grains and animal feed at levels as low as 0.01 μg/g.
Method
Analytical Conditions
Column: Mycotox reversed-phase C18, 4.6 × 250 mm
(Part No: 1612124)
Temperature: 40 °C
Flow rate: 0.8 mL/min
Mobile phase: Eluant A: dilute 1 mL of formic acid to 1 L
with D.I. water
Eluant B: MeOH
Injection volume: 10–50 μL
Analysis of Fumonisins in Grains and FeedsMaria Ofi tserova and Sareeta Nerkar, Pickering Laboratories, Inc.
Figure 1: Chromatogram of corn sample contaminated with 0.17 μg/g of FB1 and 0.03 μg/g of FB2.
Figure 2: Chromatogram of mixed feed sample contaminated with 0.08 μg/g of FB1 and 0.02 μg/g of FB2.
-- ----
--
FB1
FB2
0 2 4 6 8 10 12 14 min
---
-- ----
--
.
FB1
FB2
0 2 4 6 8 10 12 14 min
Post-Column Conditions
Post-column system: Pinnacle PCX
Heated reactor volume: 1.4 mL
Temperature: 65 °C
Reagent: 950 mL GA 104, 300 mg OPA, 2 g
Thiofl uor, 3 mL of 30% Brij 35 solution
Reagent fl ow rate: 0.4 mL/min
Detection: FLD, λEX
=335 nm, λEM
= 440 nm
Table I: HPLC Gradient
Time Eluent A, % Eluent B, %
0 45 55
2 45 55
9 30 70
14 10 90
16 10 90
16.1 45 55
22 45 55
FOOD & BEVERAGE
APPLICATION NOTES – DECEMBER 2017 61
Pickering Laboratories, Inc.1280 Space Park Way, Mountain View, CA 04043
tel. (800) 654-3330, (650) 694-6700
Website: www.pickeringlabs.com
Figure 3: Chromatogram of barley sample spiked with 0.2 μg/g of FB1 and 0.07 μg/g of FB2.
-- ----
--
FB1
FB2
0 2 4 6 8 10 12 14 min
Figure 4: Chromatogram of saffl ower seeds sample spiked with 0.2 μg/g of FB1 and 0.07 μg/g of FB2.
-- ----
--
FB1
FB2
0 2 4 6 8 10 12 14 min
Table II: Analysis of Fumonisins
SampleFB1 Found in
Sample
FB2 Found in
Sample
FB1 Spike
Concentration
FB2 Spike
ConcentrationFB1 Recoveries FB2 Recoveries
Barley 0 μg/g 0 μg/g 0.2 μg/g 0.07 μg/g 92.3% 87.5%
Milo 0.04 μg/g 0.01 μg/g 0.2 μg/g 0.07 μg/g 85.4% 81.8%
Saffl ower seeds 0 μg/g 0 μg/g 0.2 μg/g 0.07 μg/g 92.0% 80.8%
Corn 0.17 μg/g 0.03 μg/g 0.4 μg/g 0.13 μg/g 91.7% 88.7%
Oats 0 μg/g 0 μg/g 0.2 μg/g 0.07 μg/g 91.5% 83.3%
Mixed feed 0.08 μg/g 0.02 μg/g 0.3 μg/g 0.1 μg/g 88.0% 80.6%
Sample Extraction and Clean-Up
Immunoaffi nity clean-up columns: FumonitestTM (WB (Vicam)
Extraction solution: Water–methanol (20:80)
PBS solution: dilute 100 mL of 10X PBS (Vicam, P/N G1113) to 1 L
with DI water
To 25 g of fi nely ground sample add 2.5 g of NaCl and 50 mL of
extraction solution. Blend at high speed for 5 min, fi lter through
fl uted fi lter. Take 10 mL of extract and add 40 mL of PBS solution,
mix well, fi lter through microfi ber fi lter. Load 10 mL of diluted extract
to Immunoaffi nity column, let the solution pass through at the
fl ow rate about 1–2 drops/s. Wash the column with 10 mL of PBS
solution, elute with 1 mL of methanol followed by 1 mL of DI water.
Evaporate the solution to dryness under the stream of nitrogen,
reconstitute in 1 mL of methanol–water (50:50). Inject 10–50 uL.
ENVIRONMENTAL
62 APPLICATION NOTES – DECEMBER 2017
The US EPA monitors a variety of chemicals in water that
may cause harm to humans or wildlife to minimize exposure.
Method 625 was developed by the Offi ce of Science and
Technology in the Clean Water program to allow the moni-
toring of a large suite of semivolatile chemicals in waste-
water for compliance with the National Pollution Discharge
Elimination System (NPDES). NPDES is a system of permit-
ting that defi nes the characteristics of water that is released
into a waterway, defi ned by industrial category. The permit-
ting levels are set depending on the waterway’s use. If the
waterway is used for recreation or is an important wildlife
habitat, the limit may be set lower.
The original method was developed in the early 1980s and has been
updated several times since then to allow the use of more mod-
ern technology. The latest update has taken place over the last few
years and was proposed in a Method Update Rule (MUR) in 2015
which was just published in the Federal Register August 28, 2017
and became effective September 27, 2017 (1). The latest version
of the method includes a larger suite of analytes (up to 364) and
an extensive set of labeled surrogates to better monitor the method
performance throughout the sample preparation and analysis step.
This application note will present the data collected as part of
the demonstration of disk solid phase extraction validation for US
EPA method 625.1. Nine different wastewater matrices were evalu-
ated and tested against the criteria listed in Table 6 of the method.
Sample preparation was performed using the Atlantic® One-pass
system, where the water sample is passed through a solid phase
extraction (SPE) disk and carbon cartridge once, rather than twice
with a pH change between loadings. Automation of the process
was achieved using the SPE-DEX® 4790 system (superseded by
SPE-DEX 5000). Table I shows the matrix spike and matrix spike
duplicate results for a centralized waste treatment point source
(437 NPDES category) sample, for selected compounds. The spike
recovery and agreement between the duplicates was within criteria
for most all analytes. Method detection limits, initial demonstration
of compliance, and other wastewater matrices are shown in the full
application note (2).
References
(1) Method 625.1, December 14 revision, can be found in the MUR, Febru-
ary 19, 2015. Or downloaded here: https://nepis.epa.gov/Exe/ZyPDF.cgi/
P100LVHC.PDF?Dockey=P100LVHC.PDF
(2) Application Note 117, “Validation of Horizon Technology Disk Extrac-
tion Technology for US EPA Wastewater Method 625.1,” available from
www.horizontechinc.com.
Validation of Horizon Technology Disk Extraction Technology for US EPA Wastewater Method 625.1Zoe Grosser*, Alicia Cannon*, Michael Ebitson*, Melissa Lever*, Nic Rasnake†, Jessica Bowker†, Allen Fuller†, and Chris Johnson†, *Horizon Technology, and †ESC Lab Sciences
Horizon Technology, Inc.16 Northwestern Drive, Salem, NH 03079
tel. (603) 893-3663
Website: www.horizontechinc.com
Table I: Centralized waste treatment point source (437)
matrix spike and spike duplicate results
CompoundRecovery
(%)
Range
P,Ps(%)
Pass/
Fail
RPD
(%)
RPD (%)
Limit
Pass/
Fail
Fluorene 99.6 59-121 Pass 6.89 38 Pass
Hexachlorobenzene 110 D-152 Pass 9.10 55 Pass
Hexachloro-1,3-butadiene 99.6 24-120 Pass 4.09 62 Pass
Hexachloroethane 61.6 40-120 Pass 16.1 52 Pass
Indeno(1,2,3-cd)pyrene 73.5 D-171 Pass 4.54 99 Pass
Isophorone 100 21-196 Pass 2.71 93 Pass
Naphthalene 96.0 21-133 Pass 2.49 65 Pass
Nitrobenzene 94.2 35-180 Pass 0.79 62 Pass
N-Nitrosodi-n-propylamine 113 D-230 Pass 2.55 87 Pass
Phenanthrene 97.2 54-120 Pass 7.33 128 Pass
Pyrene 90.9 52-120 Pass 8.40 49 Pass
1,2,4-Trichlorobenzene 90.2 44-142 Pass 5.68 50 Pass
PHARMA/DRUG DISCOVERY
APPLICATION NOTES – DECEMBER 2017 63
A revolutionary new inline static mixer has been developed and
specifi cally tailored to meet the exacting demands of HPLC and
UHPLC systems. Poor mixing of two or more mobile phase sol-
vents results in a high signal to noise ratio and, thus, decreased
sensitivity. The test data presented demonstrates that superior
mixing is achieved while minimizing the internal volume in var-
ious gradient test conditions, such as unparalleled mixing for
trifl uoroacetic acid (TFA) and water and acetonitrile gradients.
Low mixing effi ciency, resulting in poor signal-to-noise ratios, has
plagued the chromatography world when it comes to limits of
detection and sensitivity. If poor mixing is present, baseline noise
will appear as a sine wave (rise and fall) of the detector signal
versus time. Poor mixing will also broaden and create asymmetrical
peaks resulting in reduced column effi ciency and peak resolution.
The ideal static mixer will combine the advantages of high mixing
effi ciency, low dead volume and low pressure drop, while minimizing
the volume and maximizing the throughput of the system.
Experimental Conditions
The following HPLC conditions and test setup were employed to
measure the system baseline noise and to compare the relative
performance of various static mixers. Figure 1 shows a schematic
diagram of a typical HPLC and UHPLC system confi guration. Static
mixer testing was performed by locating the Mott PerfectPeak® static
mixer immediately downstream of the pump and upstream of the
sample injector and HPLC column. For this study, mixing effi ciencies
were performed by bypassing the sample injector and column using
a capillary tube between the static mixer and the UV detector to
create back pressure.
The HPLC system utilized for this testing was an Agilent 1260
Series HPLC with a UV detector controlled using Agilent Chemstation
Software. Table I contains the setup conditions used in this study.
Conclusion
This application note demonstrates that greater mixing effi ciency
is exhibited with every Mott PerfectPeak® static mixer volume
increase with respect to the system with no mixer. Greater than
95% reduction in baseline noise was achieved with the largest size
mixer which will result in improved limits of detection and sensitivity.
Reducing HPLC and UHPLC System Noise and Volume with
High-Performance Static Mixers
James K. Steele, PhD, Kenneth L. Rubow, PhD, and Christopher J. Martino, Mott Corporation
Mott Corporation84 Spring Lane, Farmington, CT 06032
tel. 1 (860) 747-6333; email: [email protected]: www.mottcorp.com
Figure 1: Schematic diagram of the low-pressure gradient experimental test system.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0 1 2 3 4 5
Ab
sorb
an
ce (m
AU
)
Time (Minutes)
Relative Performance - Mott 25, 50, 100, 150, & 300 !L
Mott 300 �L
Mott 150 �L
Mott 100 �L
Mott 50 �L
Mott 25 �L
No Mixer
Figure 2: Plots showing offset HPLC UV detector signal versus time for this study showing baseline noise with no mixer, and Mott 25 μL, 50 μL, 100 μL, 150 μL, and 300 μL mixers.
Table I: HPLC configuration
Pump speed 0.25 mL/min through 1.0 mL/min
Solvent A 20 mM ammonium acetate in DI water
Solvent B 80% Acetonitrile (ACN) / 20% DI water
Solvent ratio 20% A / 80% B
Detector 220 nm
Flow rate 0.5 mL/min
POLYMER
64 APPLICATION NOTES – DECEMBER 2017
Phthalates are widely used as plasticizers in different industries to
enhance product durability. It has been determined that many of the
phthalates have a detrimental effect on human health. Six phthalates
(shown in Table I) are now regulated by the EU (Directive 2005/84/EC)
and the US (Consumer Product Safety Improvement Act, Section 108).
All the past methodologies for the determination of these phthalates
are based on solvent extraction, fi ltration, and concentration. These tra-
ditional techniques are cumbersome, time consuming, and suffer from
analyst-to-analyst variability while producing data of limited value.
In response to these analytical challenges and in conjunction with
the ASTM, Frontier Laboratories developed a technique using the Multi-
Shot Pyrolyzer coupled with a GC–MS system for analyzing phthalates
in PVC. This technique provides scientists with a “method map” for
identifying phthalates both qualitatively and quantitatively. First, Evolved
Gas Analysis (EGA)-MS is used to determine the optimal thermal zone
in which the target compounds evolve as the sample is heated. Then,
a simple, one-step thermal desorption (TD)-GC–MS technique is used
for the analysis. This application note details the fi rst step of the “meth-
od map” for identifying the thermal desorption temperature zones of
phthalates in PVC.
A sample consisted of small pieces of a PVC toy was taken from 10
different locations. They were dissolved in 1 mL of THF (50 mg/mL). Six
different phthalates were added to the THF solution. 10 μL of the THF
spiked solution was added to a sample cup; the solvent was evaporated
leaving a thin fi lm of the sample on the surface of the cup (ca. 0.5 mg).
Figure 1 shows the obtained EGA thermogram and extracted ion
chromatograms (EICs) that are used to determine the thermal zone for
each of the phthalates and the hydrogen chloride that is generated as
PVC is heated. Using average mass spectrum, the fi rst peak is identifi ed
as DINCH (1,2 cyclohexane dicarboxylic acid di-isononyl ester). It is
critical to ensure that the ions used to monitor the six phthalates can be
differentiated from ions produced by non-target additives, like DINCH.
The EICs clearly show that the temperature range in which all six phthal-
ates are thermally desorbed is 100 to 350 ºC.
Pyrolyzer: 100–600 ºC (20 ºC/min)
GC oven temp.: 300 ºC,
EGA tube: UADTM-2.5N (L=2.5 m, i.d.=0.15 mm)
Column fl ow rate: 1 mL/min; He, Split ratio: 1/20
Inj. port temp.: 320 ºC
This work clearly shows that the Frontier Multi-Shot Pyrolyzer is a viable
technique in analyzing phthalates in many industries. Today, more
laboratories are integrating the Frontier Multi-Shot Pyrolyzer into their
mainstream analytical protocols due to the advantages and immediate
quality improvements this technique provides. The Frontier Multi-Shot
Pyrolyzer guarantees reproducibility and accuracy while all surfaces
in the sample path are quartz deactivated using a proprietary process.
There is NO transfer line and NO cross contamination. The system also
increases laboratory productivity as sample preparation takes much
less time than traditional techniques that require solvent extraction.
Analysis of Phthalates in Polyvinyl Chloride (PVC) by Thermal
Desorption GC–MS: Determination of Thermal Desorption
Temperature Zone by Evolved Gas Analysis (EGA)Rojin Belganeh and William Pipkin, Frontier Laboratories (Koriyama, Japan)
Quantum AnalyticsFoster City, California, United States
tel. (650) 312-0900
Website: http://go.lqa.com/pyrolyzer-info Figure 1: TIC thermogram and extracted mass chromatograms of PVC, to which 1% of each phthalate was added
Table I: Use restricted phthalates (1% upper limit by
directive 2005/84/EC
Di(2-ethylhexyl) phthalate (DEHP)
Dibutylphthalate (DBP)
Butylbenzylphthalate (BBP)
Diisononylphthalate (DINP)
Diisodecylphthalate (DIDP)
Di(n-octyl)phthalate (DNOP)
GENERAL
APPLICATION NOTES – DECEMBER 2017 65
The Hamilton PRP-C18 is a column designed to provide high-
effi ciency reversed phase separations over an extended col-
umn life in nearly any mobile phase or pH. The rigid sta-
tionary phase has excellent mechanical and thermal stability
(> 100 °C), does not experience shrinkage or swelling and is
completely inert to most conditions commonly encountered
in reversed phase chromatography. In this study the pH sta-
bility of the PRP-C18 is evaluated. Even after prolonged ex-
posure to concentrated (1 molar) NaOH and H2SO4 there was
no measurable deterioration in performance.
Octadecyl silane (ODS) is the prevailing stationary phase in reversed-
phase HPLC. Despite a well-celebrated, widespread use in chroma-
tography, traditional ODS columns are not without limitations. Acidic
conditions promote hydrolytic stripping of octadecyl functionalization,
while alkaline conditions (pH > 7) attack the silica bed, both of which
are principle sources for anomalous peak shape and shifting retention
times that progressively worsen over the life of the column.
The PRP-C18 reversed phase column has similar mechanical
stability (up to 5000 psi) and separation effi ciency to that of tradi-
tional ODS, but without many of the chemical restrictions. The PRP-
C18 stationary phase does not experience stripping or dissolution
under the most extreme conditions. This allows for an expanded
mobile phase repertoire for use in methods development or aggres-
sive regeneration procedures.
Experimental Conditions
Column: PRP-C18, 4.1 × 50 mm, 5 μm
Instrumentation: Agilent 1100 quaternary pump with UV detector
Standards: acetone, phenol, benzyl alcohol, benzene, toluene, ethyl-
benzene, propylbenzene, napthalene, pentylbenzene, hexylbenzene
Mobile phase A: 0.2% Phosphoric acid
Mobile phase B: A + 95% ACN
Gradient: 5 to 100% B in 20 min
Flow rate: 2 mL/min
Temperature: Ambient
Injection volume: 2 μL
Detection: UV at 205 nm
Conclusion
Mobile phase pH is a useful tool in methods development, particularly
for separation of neutral forms of amines or other organic bases under
alkaline conditions. Although a few more recent C18 columns boast sta-
bility in alkaline pH, column life is still considerably shorter than if used
under more favorable conditions. On the other hand, the PRP-C18 has
genuine pH and chemical stability. The stationary phase is devoid of
free silanols, does not strip, bleed, or dissolve at any pH, and therefore
can be expected to perform reliably and reproducibly throughout the
extended life of the column, regardless of mobile phase conditions.
Hamilton PRP™-C18
High-Effi ciency Separations at Any pHDerek Jensen and Mark Carrier, Hamilton Company
Hamilton Company4970 Energy Way, Reno, NV 89502
tel. (800) 648-5950, fax (775) 858-3026
Website: www.hamiltoncompany.com
Initial
After 1 M NaOH
After 1 N H2SO
4
0 10 20
Figure 1: Separation of standard mix after 200 column volumes 1 M NaOH, 1 M H2SO4.
THE APPLICATION NOTEBOOK
Call for Application Notes
LCGC is planning to publish the next issue of
Th e Application Notebook special supplement in
February. Th e publication will include vendor
application notes that describe techniques and
applications of all forms of chromatography
and capillary electrophoresis that are of im-
mediate interest to users in industry, academia,
and government. If your company is interested
in participating in these special supplements,
contact:
Edward Fantuzzi, Publisher,
(732) 346-3015
Brianne Molnar, Sales Manager,
(732) 346-3034
Stephanie Shaff er, Sales Manager,
(774) 249-1890
Application Note Preparation
It is important that each company’s mate-
rial fi t within the allotted space. Th e editors
cannot be responsible for substantial editing
or handling of application notes that deviate
from the following guidelines:
Each application note page should be no more
than 500 words in length and should follow
the following format:
• Title: short, specifi c, and clear
• Abstract: brief, one- or two-
sentence abstract
• Introduction
• Experimental Conditions
• Results
• Conclusions
• References
• Two graphic elements: one is the company
logo; the other may be a sample chromato-
gram, fi gure, or table
• Th e company’s full mailing address,
telephone number, fax number,
and Internet address
All text will be published in accordance with
LCGC ’s style to maintain uniformity through-
out the issue. It also will be checked for gram-
matical accuracy, although the content will not
be edited. Text should be sent in electronic for-
mat, preferably using Microsoft Word.
Figures
Refer to photographs, line drawings, and
graphs in the text using arabic numerals in
consecutive order (Figure 1, etc.). Company
logos, line drawings, graphs, and charts must
be professionally rendered and submitted as
.TIF or .EPS fi les with a minimum resolution
of 300 dpi. Lines of chromatograms must be
heavy enough to remain legible after reduc-
tion. Provide peak labels and identifi cation.
Provide fi gure captions as part of the text,
each identifi ed by its proper number and title.
If you wish to submit a fi gure or chromato-
gram, please follow the format of the sample
provided below.
Tables
Each table should be typed as part of the main
text document. Refer to tables in the text by
Roman numerals in consecutive order (Table
I, etc.). Every table and each column within
the table must have an appropriate heading.
Table number and title must be placed in a
continuous heading above the data presented.
If you wish to submit a table, please follow
the format of the sample provided below.
References
Literature citations must be indicated by ara-
bic numerals in parentheses. List cited refer-
ences at the end in the order of their appear-
ance. Use the following format for references:
(1) T.L. Einmann and C. Champaign, Science
387, 922–930 (1981).
Th e deadline for submitting application notes for the February issue of Th e Application Notebook is:
January 13, 2018
Th is opportunity is limited to advertisers in LCGC North America. For more information, contact: Ed Fantuzzi at (732) 346-3015,
Brianne Molnar at (732) 346-3034, or Stephanie Shaff er at (774) 249-1890
Table I: Factor levels used in the designs
Factor Nominal Value Lower Level (−1) Upper Level (+1)
Gradient profile 1 0 2
Column temperature (°C) 40 38 42
Buffer concentration 40 36 44
Mobile-phase buffer pH 5 4.8 5.2
Detection wavelength (nm) 446 441 451
Triethylamine (%) 0.23 0.21 0.25
Dimethylformamide 10 9.5 10.5
Figure 1: Chromatograms obtained us-ing the conditions under which the ion suppression problem was originally dis-covered. The ion suppression trace is shown on the bottom. Column: 75 mm × 4.6 mm ODS-3; mobile-phase A: 0.05% heptafl uorobutyric acid in water; mobile-phase B: 0.05% heptafl uorobutyric acid in acetonitrile; gradient: 5–30% B in 4 min. Peaks: 1 = metabolite, 2 = internal standard, 3 = parent drug.
66 APPLICATION NOTES – DECEMBER 2017
8VH�3URPR�&RGH�&6&���IRU�����2II�5HJLVWUDWLRQ�
Hosted by
dŚĞ tŽƌůĚ�Ɛ >ĂƌŐĞƐƚ �ĂŶŶĂďŝƐ ^ĐŝĞŶĐĞ �ŽŶĨĞƌĞŶĐĞƌĞƚƵƌŶƐ ƚŽ ĚŽǁŶƚŽǁŶ WŽƌƚůĂŶĚ ĨŽƌ ĂŶ ŝŶĐƌĞĚŝďůĞ ŐĂƚŚĞƌŝŶŐ ŽĨ ĂŶĂůLJƟĐĂů
ƐĐŝĞŶƟƐƚƐ� ŵĞĚŝĐĂů ƉƌŽĨĞƐƐŝŽŶĂůƐ� ĐĂŶŶĂďŝƐ ŝŶĚƵƐƚƌLJ ĞdžƉĞƌƚƐ ĂŶĚ ŶŽǀŝĐĞƐ
ŝŶƚĞƌĞƐƚĞĚ ŝŶ ůĞĂƌŶŝŶŐ ŵŽƌĞ ĂďŽƵƚ ĐĂŶŶĂďŝƐ ƐĐŝĞŶĐĞ�
^ƉŽŶƐŽƌƐŚŝƉ�ĂŶĚ�ĞdžŚŝďŝƟŽŶ�ŽƉƉŽƌƚƵŶŝƟĞƐ�ĂƌĞ�ƐƟůů�ĂǀĂŝůĂďůĞ���ŽŶƚĂĐƚ�:ŽƐŚ�
�ƌŽƐƐŶĞLJ�Ăƚ�ũŽƐŚΛũĐĂŶŶĂ�ĐŽŵ ĨŽƌ�ŵŽƌĞ�ŝŶĨŽ�
M&DQQD,QF
M&DQQDB,QF
#M&DQQDB,QF
&DQQDELV6FLHQFH&RQIHUHQFH�FRP
3RUWODQG��2UHJRQ