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Copy Number MIRU-VNTR ESM Part 3 An example of a report of 24 MIRU-VNTR results. Report summary: the two strains 1396-09 and 0333-10 have identical MIRU-VNTR profiles, which differ from the MIRU-VNTR profiles of the other three strains. The 24 MIRU-VNTR typing pattern for reference strain MT14323 is a quality assurance control from each PCR and corresponding electrophoresis plate Locus: 2 4 10 16 20 23 24 26 27 31 39 40 A B C M 04 M 21 M 29 M 30 M 34 M 39 Q 11b 4052 4156c ID 1024-08 2 1 5 1 2 5 1 1 3 3 2 2 3 2 4 3 2 4 4 3 3 2 7 3 0364-09 2 1 5 1 2 5 1 1 3 2 2 2 3 2 4 4 3 4 4 3 3 2 7 3 1396-09 2 1 5 1 2 5 1 1 3 3 2 2 3 2 4 4 3 4 4 3 3 2 7 3 0333-10 2 1 5 1 2 5 1 1 3 3 2 2 3 2 4 4 3 4 4 3 3 2 7 3 0482-10 2 1 5 1 2 5 1 1 3 3 2 2 3 2 4 5 3 4 4 3 3 2 7 1 Referenc e MT14323 2 2 5 3 1 3 1 5 3 3 1 1 3 2 3 2 3 2 4 3 3 2 7 3 ESM Part 3 Journal: ACQUAL publisher: Springer year:2011 title: Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterium reference laboratory authors: Freidlin PJ*, Goldblatt D, Kaidar-Shwartz H, Dveyrin Z, Rorman E * corresponding author: [email protected]

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MIRU-VNTR

ESM Part 3 An example of a report of 24 MIRU-VNTR results. Report summary: the two strains 1396-09 and 0333-10 have identical MIRU-VNTR profiles, which differ from the MIRU-VNTR profiles of the other three strains. The 24 MIRU-VNTR typing pattern for reference strain MT14323 is a quality assurance control from each PCR and corresponding electrophoresis plate

Locus:2410162023242627313940ABC

M 04

M 21

M

29

M 30

M 34

M 39

Q 11b

40524156c

ID

1024-08215125113322324324433273

0364-09215125113222324434433273

1396-09215125113322324434433273

0333-10215125113322324434433273

0482-10215125113322324534433271

Reference MT14323

225313153311323232433273

ESM Part 3 Journal: ACQUAL publisher: Springer year:2011 title: Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterium reference laboratory authors: Freidlin PJ*, Goldblatt D, Kaidar-Shwartz H, Dveyrin Z, Rorman E * corresponding author: [email protected]