Conversion c3 to c4 Plant
Transcript of Conversion c3 to c4 Plant
The major reason:- C4 plant have desirable agronomic traits High photosynthetic capacity High water and nitrogen use efficiencies
(leads to rapids growth rates, higher dry matter production and larger economic yields)
Detractive effects of photorespiration
A. Bandyopadhyay, K. Datta, J. Zhang, W. Yang, S. Raychaudhuri, M. Miyao
(2007)
Related journal to the topic
OBJECTIVES Introduce enzymes involved in C4 plant
photosynthesis into C3 plant of rice Determine the effects on photosynthesis
and plant productivity
What is PEPC gene? Phosphoenolpyruvate carboxylase (PEPC)
gene The gene known to play the key role in C4
photosynthesis by catalyzing initial fixation of CO2 in C4 maize
2) MEASUREMENT OF 2) MEASUREMENT OF PHOTOSYNTHESISPHOTOSYNTHESIS
AND AGRONOMIC DATAAND AGRONOMIC DATA
300 putative transgenic hygomycin resistant plant.
9% of the transformants showed abnormal morphological and physiological.
Genomuc DNA from PCR positive T3 plant showed the presence of non-segregating band of desired sized.
Twenty T1 progenies from the four selected T0 plant were analyzed for the mode of inheritance and segregation of the transgene.
PCR and Southern blot analysis of twenty T1 plant derived from each transgenic line showed the Mendelian segregation of 3:1
Why we need transferring the C4 traits into rice?
Why we use PEPC gene ? Rice is the C3 plant. So, how the C3 can
be C4 plant?