Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2
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Transcript of Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2
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Control of Anthracnose of banana (Kluai Khai ) by the
biofumigant fungus Musco dor albus strain MFC2
Kusuma PotchanaWorraya
PrompangtaChomporn
RaksakiettisakAdviser Miss Aree Sakyim
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Purposes• To test the efficiency of MFC2 to inhibit
the anthracnose pathogen in banana. • To find the proper nutrient for MFC2Research Boundary• Testing MFC2 with two pathogen ;
Colletotrichum capsici and Colletotrichum gloeosporioidesระยะเวลาที่�ที่�าการ
ศึ�กษา • Twelve months (November 2006 - November 2007)
Term of Research
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Laboratory
• Biotechnological laboratoy of Mahidol University• Biological laboratory of Mahidol Wittayanusorn
School
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Method
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• Equipment and Substances •Colletotrichum capsici •Colletotrichum gloeosporioides•Muscodor albus strain MFC2•Autoclave• Larminar flow• Pasteur pipettes
ว�ธีดำ�าเนิ�นิการที่ดำลองMethod
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• Part 1 Grow MFC2 on PDA
Do hyphal tip with MFC2
Grow MFC2 for 10 -14 days
Method
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•Make nutrients for growing MFC2
Nutrient 1 Nutrient 2
Brown rice 15 grams
millet 35 grams
Bran 5 grams water 5 cm2
Method
millet 50 grams
Bran 5 grams water 5 cm2
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• Part 2 Grow Pathogens
Method
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• Part 3 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on PDA
10 day control
7 day
5 day
Method
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• Part 4 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on nutrient 1 and 2
7 day 7 day
ชุ�ดควบค�ม
Method
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Results
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Results
Table 1 Test on PDA
Plate Diameter (cm)
C.gloeosporioides
C.capsici
1 3.7 1.2
2 3.7 1.6
3 3.6 2.7
Average 3.67 1.83
Control 5.3 7
Pathogens after 5
day
inhibit inhibit
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• Table 2 Test on nutrient 1
Plate Diameter (cm)
C.gloeosporioides
C.capsici
PDA
NoPDA
PDA NoPDA
1 2.4 3.9 0.4 1
2 2.8 1 0.4 3
3 1.25
4.2 1 2.5
Average
2.15
3.03 0.6 2.17
Control 4.4 4.4
Pathogen after 5 days
Inhibit Inhibit
Results
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• Table 3 Test on nutrient 3 Plate Diameter (cm)
C.gloeospoiroides
C.capsici
PDA
NoPDA
PDA NoPDA
1 3.1 4.3 0.4 0.4
2 2 1.9 3.2 2.7
3 1 3 1.5 3.4
Average
2.03
3.06 1.7 2.17
Control 4.4 4.4
Pathogen after 5 days
Inhibit inhibit
Results
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• Muscodor albus Strain 2MFC growed in two different nutrients in order to control two anthracnose pathogens of banana ;Colletotri
chum capsici and Colletotrichum gloeosporioides can inhibit the two pathogens but does not make any difference. Considered with the quantity of the nutrients, MFC2 growed on nutrient mixed with MFC2 has more efficiency to control the pathogens than another which is growed without PDA.
Results
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Conclusions and Analysis
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การว�เคราะห์�ค�าความแตกต�าง
Conclusions and Analysis
• The nutrient ; water, bran, brown rice and millet, mixed with PDA can grow the MFC2 well and costs low. So this is another way to grow MFC2 to inhibit the two anthracnose pathogens of banana;
Colletotrichum capsici and Colletotrichum gloeosporioides.
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Advice
การว�เคราะห์�ค�าความแตกต�าง
• Test with banana, let farmer use and assess.
• Find the proper temperature for growing MFC2.
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• 2004Ezra, D., W.M. Hess and G.A. Strobel. . New endophytic is MMMMMMMM MMMMMM M MMMMMMMM-MMMMMMMMMM-MMMMMMMMM MMMMMMM,. Microbiology . 150: 4023–4031.
• Freeman, S., Y. Nizami, S. Dotan, S. EvenandT. Sando. 1997. Cont r ol of Collectotrichum acutatum MMMMM MMMMMMMMMMM MMMMMMMMMMM MMM MMMMM M,,
. Plant Disease -1997: 749 752.• MM MMMMM MMMMMMMMMMM MMMMMMMMM , .. 2000. , Colletotrichumgloeosp
orioides , to the Fungicide Prochloraz. Proc. Natl. Sci. Counc. ROC(B) -25 174179vol. : .
• 2003Mercier, J. and J.I. Jiménez. . Control of fungal decay of ap pl es and peaches by t he bi of umi gant f ungus Muscodor albus. Po
stharvest Biology and Technology. 31 1 8: – .
Reference
• 200Strobel, G.A., E. Dirkse, J. Sears and C. Markworth. 1 . Volatile antimicrobials from Muscodor albus M MMMMM MMM,
MMMMMMM . Microbiology 147 2943 2950. : – .• , .. 1980. The Coelomycetes, Fungi Imperfecti with Pyc
nidia, Acervuli and Stromata MMMMMMMM . ,.