Continuing from last time: Receptors 2 types of acetylcholine receptors: 2 types of glutamate...
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Transcript of Continuing from last time: Receptors 2 types of acetylcholine receptors: 2 types of glutamate...
Continuing from last time: Receptors
2 types of acetylcholinereceptors:
2 types of glutamate receptors
same liganddifferent response
Explain response
Adapted from fig 21-40
Non-NMDA
Glutamate
NMDANa+ Na+
Na+ Na+
Mg++
Removal of Mg++
Na+
Ca++
Na+
Ca++
Coincidence detector
Moving On:
Neurotransmitters Neurotransmitter receptors
Soluble Transmembrane
Synaptic signaling requires protein receptorsand peptide- derived signals
How do you make a protein?How do you get a protein where it needs to be?
Questions
What is the central dogma?
How would a neuropeptide get made (in general terms)?
What are the basic parts of DNA, RNA, and proteins?
What is the difference between hnRNA, mRNA and
tRNA?
Part I: soluble neuropeptide –From DNA to protein
Genes
‘ The entire nucleic acid sequence necessary for thesynthesis of a functional polypeptide’
MCB, p285
Components of a Eukaryotic Gene
What are the ‘parts’ or regions of a gene?How are they organized?
Do genes always encode RNAs?
Do genes always encode RNAs that encode proteins?
Questions
How does RNA polymerase work and what does it make? How does it know where to start and stop? How does a ribosome work and what does it make? How does it know where to start and stop? If the DNA in every cell in your body is the
same why don't your adipose (fat) cells secrete epinephrine?
If the DNA contains all of the information why doesn't the ribosome just 'read' it?
Why have intermediate steps?
Looking at RNA ECB2 7.1
Making a cytosolic protein: Step 1--transcribe
Genes encode proteins
Why use an RNA step?
Major steps in process: Initiation, Elongation and Termination animation
DNA-RNA interactionsDNA Template strand Complementary RNA strand
DNA/RNAHybrid
Adapted from Life; Purves 6thed
WatchingTranscriptionEBC2 7.2
http://www.johnkyrk.com/DNAtranscription.htmlhttp://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf
Check out