Constructing E.coli cells to act like multicellular organisms ——The engineered evolutionary...
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Transcript of Constructing E.coli cells to act like multicellular organisms ——The engineered evolutionary...
Constructing E.coli cells to act like multicellular organisms
——The engineered evolutionary story
Approaching to this purpose
• One-dimension
• Two-dimension
• Three-dimension
• Mathematical modeling
Do E.coli cells have a head?
One-dimension organization
• The simplest form----two cells
What we need are:
1) A protein located at the anterior side of E.coli cells.
2) A self-recognizable domain that act both as a ligand and a receptor.
The anterior located protein
• Chemoreceptor Complex in Escherichia coli.
Tsr
CheA
CheW
The outer membrane domain can be modified
• By the monomer of leucine zipper or the IgSF domain.
• By the hexomer of histidine.
Two-dimension organization
What we need are:
1) A protein located in the anterior site that can bind to a shaped mode.
2) A membrane protein that is broadly distributed on the surface of E.coli cells.
OmpC protein
• An outer membrane protein.
Cell Division Control
• SulA and MinCD
• Enrolled in SOS.
Three-dimension: the story of evolution
• When cell density increases:
• Expression of modified OmpC via quorum sensing system.
(eg. Inserting constructed OmpC sequence after the promoter of LuxR)
• Randomly bind?
Mathematical modeling
• What distinguishes multicelluar organisms from unicellular ones.
• Evolutionary fitness and trade-off.