AgricAgric oo Biotech Biotech

I.V. ShalnovI.V. Shalnov

Agricultural Biotechnology Laboratory of the Tver State Agricultural Agricultural Biotechnology Laboratory of the Tver State Agricultural AcademyAcademy

The organization of the complex for the production The organization of the complex for the production of disease-free seedof disease-free seed

crop materialcrop material

Currently, the agro-industrial complex of the Russian Federation, and in particular the Tver region has created a situation in which much of the production of agricultural products at a loss. Potatoes - one of the few crops grown in our region and are cost effective.

Our country is a major exporter of potatoes and is one of the leading places in the world, both in areas under crops and the gross collection, yielding to these measures only to China. Recently, however, our country is bought about 350 tons per year, and the first seeds of elite reproduction. This suggests that the insufficient supply of domestic producers of potato quality and relatively inexpensive seed material.

Reproduction in potato production is vegetatively (tubers), ie create clones of plants with absolutely identical set of genetic characteristics.

Within a few years vegetation in plants generated and transmitted to subsequent generations certain changes caused mycoplasma, virus and viroid diseases.Crop losses caused by a viral infection in different cultures and in different climatic conditions, reach a considerable size. For example the potato virus diseases reduce yield by 20-60%.

Country Yields reproductions% of the elite

I II III IV V

Russia 95 90 85 80 75

Byelorussia 90 80 68 65 52

Dynamics of potato yield in the reproductionsin different zones

The progress made in recent years, biotechnology, have created the possibility of solving scientific problems of a new branch of modern agricultural production - disease-free seed.

Are special innovative manufacturing companies in which

produced the "purification" of the material from pathogens. To do this, the complex of measures ensuring the complete removal of visible and hidden sources of contamination of the seed with a further reproduction in laboratory conditions and adaptation to production conditions.

We suggest the use of known technologies, and we have developed unique methods of production of seed potato virus-free basis under in vitro (in glass).

The purpose of the enterprise will be opened production of virus-free seed potatoes, based on our developed technology of cultivation.

Improvement of disease-free planting material will be carried out in several stages:

Stage 1Preparing for the isolation of tuber apical meristem or sprouts from a

known healthy plants

Stage 2Sterilization of plant material and the isolation of the apical meristem or

sprouts aseptically

Stage 3Cultivation of explants on artificial media in a room with controlled

lighting and temperature conditions

Stage 4Cuttings obtained regenerated plants by the number of internodes and

cuttings for planting medium

Stage 5Production of mini-tubers in the aeroponic greenhouse

Currently, a patent on the technology of breeding disease-free planting potatoes:

"The method of cultivating plants using aeroponics and ultrasonic device for their cultivation"

Fig. Scheme of ultrasonic aeroponic setup:1 - nutrient solution tank 2 - nutritive solution;3 - tee, 4 - Ultrasonic fog generator;5 - the capacity to feed and maintain the level of the solution; 6 - flowguide 7 - plants, 8 - mounting location of plants;9 - pipeline, 10 - flyanets seal, 11 - open hatches;12 - fan.

Further steps are breeding industrial field:

1. The landing of mini-tubers in the field using clonal potato planter and receipt of the first field of the reproduction of potatoes;

2. Reproduction of the material and getting super-super-elite;3. Reproduction of the material and getting super-elite;

The approximate amount of investment for the organization of the laboratory(excluding the value of the premises)

№ Тame Amountthous. rub.

The investments 1. Equipment washing room 50

2. Equipment sterilization room 50

3. Equipment operating room 170

4. Equipment phytotron 180

5. Reagents and Consumables 90

6. Labware 40

Only: 540

Required investments 7. Setting an aeroponic Aero-Flo 150

8. Equipment aeroponic greenhouses 200

9. Additional laboratory equipments 200

10. Reagents and Consumables 60

Only: 400

IN TOTAL: 1190