Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

Comparative Analysis

The VENTANA HE 600 system versus a traditional linear stainer

Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

Study participantDr. Sandra Mueller, PETROGLYPH Pathology Services• Comparison of H&E stain quality between the VENTANA HE

600 system and a traditional linear “dip and dunk” stainer (Leica Autostainer XL)

• Dr. Mueller evaluated 480 slides total– 240 slides per instrument - 40 slides per instrument per tissue

sample• 6 tissue samples: Lymphoma 1, Lymphoma 2, Melanoma, Gastric Ca,

Lobular Ca, Liver– H&E stain quality assessed with four-point scale shown below

with Dr. Mueller’s definition for each rating:

Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

ResultsThe VENTANA HE 600 system improves H&E stain quality

3.8Average quality

score out of 4.0

3.0

The VENTANA

HE 600 system

Average quality

score out of 4.0

Dip and dunk

stainer

Average H&E stain quality scores across all slides and tissue samples

Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

ResultsThe VENTANA HE 600 system improves H&E stain quality

85%rated excellent quality

26.2%

The VENTANA HE 600

system

Dip and dunk stainer

rated excellent quality

0%rated poor quality

6.7%rated poor quality

Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

ResultsMore slides rated excellent on the VENTANA HE 600 system

100%more stains

rated excellent

77.5% 60%

Gastric Ca Lymphoma 1

more stains

rated excellent

more stains

rated excellent

Liver

45%more stains

rated excellent

40% 30%

Lobular Ca Melanoma

more stains

rated excellent

more stains

rated excellent

Lymphoma 2

58.8%more stains rated

excellent overall

“Why should pathologists refuse to settle for good enough? Because patients deserve more than good enough! As physicians, we should strive for excellence in patient care by asking how we can make it better.”– Dr. Sandra Mueller, pathologist

Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

Impressions on H&E stain quality consistencyA sampling of quotes from Dr. Mueller

The VENTANA HE 600 system Dip and dunk stainer• “This is very pretty tissue!

Consistent. Great detail. Nice H&E balance.”

• “Hematoxylin is very crisp, which highlights all the nuclear features.”

• “VERY consistent staining. Very crisp detail.”

• “This is a great stain. Beautiful contrast! Very consistent.”

• “Tissue has an edge effect…eosin is less differentiating…has a basophilic hue compromising degree of contrast.”

• “Eosin is lacking…hematoxylin lacks crispness.”

• “Eosin is inconsistent…hematoxylin has pink hue in many nuclei.”

• “Good stain…not great contrast.”

“On the VENTANA HE 600 system, eosin was more differentiating, which makes low power magnification more effective in achieving a diagnosis.”– Dr. Sandra Mueller, pathologist

Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.

Individual slide staining improves performanceEliminates reagent carry over and other stain quality defects

“Depletion, dilution, evaporation and oxidation – all commonplace with dip and dunk methods – are completely resolved with individual slide staining on the VENTANA HE 600 system. Fewer defects means fewer distractions, decreased eye fatigue and improved diagnostic performance.”– Dr. Sandra Mueller, pathologist

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