COMATOGAP -...

CHROMATOGRAPHY

Think about foodCare about safety

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot DeutschlandFrankreichMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-Mail sales-frmn-netcom

SchweizMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-Mail sales-chmn-netcom

Deutschlandund InternationalTel +49 24 21 969-0Fax +49 24 21 969-199E-Mail infomn-netcom

MACHEREY-NAGEL

EN ISO 9001 2008ZERTIFIZIERT

USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-Mail sales-usmn-netcom

Vol 3Antibiotics

infomn-netcom wwwmn-netcom2 3

Focus on antibiotics The global production and trading of food and feed products makes monitoring and control difficult

Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life

MN shows examples for food analysis that will help you with your work and make our food safer

Vol 3 Antibiotics in food of animal origin

Healthy animals provide healthy food

Antibiotics or antimicrobials are primarily used to treat infections caused by bacteria and other microorganisms but also they show growth promoting effects

In order to have the possibility to prevent diseases a ju-dicious use of antimicrobials is necessary since bacteria can be changed and then antibiotics are no longer effec-tive in people who need them to treat infections Antibiot-ic resistance is now a major threat to public health[WHO ndash World Health Organisation ndash httpwwwwhointmediacentrenewsreleases2014amr-reporten]

There are different classes of antimicrobials egbullPenicillinsbullAminoglycosidesbullArsenicalsbullPolypeptidesbullGlycolipidsbullTetracyclinesbullMacrolidesbullLincosamidesbullβ-LactamsbullQuinolones fluoroquinolonebullStreptograminsbullSulfonamidesbulland others

[Wikipedia ndash httpenwikipediaorg wikiList_of_antibiotics]

Most of these examples are antibiotics that were first iso-lated from a natural source Nowadays they are mostly produced synthetically and new antibiotics are usually semisynthetic modifications

Most of the antibiotics used in food animals are the

same as those used in humans

Due to the importance of antibiotic resistance as a public health problem

many governments and professional societies have reviewed existing scien-tific evidence and developed recommen-dations to limit all types of antibiotic use including use in food animals [National Anti-

microbial Resistance Monitoring System (NARMS) ndash FDA httpwwwfdagovAnimalVeterinarySafety

HealthAntimicrobialResistanceNationalAntimi-crobialResistanceMonitoringSystemdefaulthtm

ndash CDC httpwwwcdcgovnarms ndash USDA httpwwwarsusdagovsaabearnarms]


Compounds of interestChemical structures of important antibiotics

QuinolonesCiprofloxacin (R = H)

Enrofloxacin (R = C2H5)COOH

F

NO

N

NR

Norfloxacin (R = H)

Pefloxacin (R = CH3)

H5C2

NO

NCOOH

F

N

RH

Miloxacin (R = OCH3)

Oxolinic acid (R = C2H5) COOH

R

O

N

OO

Nalidixic acid

COOH

H5C2

H3CO

NN

Sarafloxacin

NH ndash NCOOH

F

F

O

N

Flumequine

COOH

CH3

FO

N

Macrolides Erythromycin Spiramycin

O

CH3

CH3OCH3

O HO

H3C

ON(CH3)2

O HO

H3C

H3CH3CH5C2 O

O

CH3

CH3

CH3OHOHHO

O

OO

O

O

O

O O

OH

N

N

H3C

H3C

H3C

H3CO

CH3

O OH

OH

CH3

H3C

HOCH3

CH3

CH3

CH3

O

β-Lactams Ampicillin Amoxycillin Penicillin G

O

OO

N

S

NH NH2

H3CH3C

HOO

HO

OO

N

S

NH NH2

OH

H3CH3C

O

OO

N

S

NH

H3CH3C

HO

Miscellaneous Chloramphenicol Thiamphenicol Florfenicol

O2N HO O

HN

OH Cl

ClS HO O

HN

OH

H3C

Cl

Cl

O OS F O

HN

OH

H3C

Cl

Cl

O O

Trimethoprim Furazolidone Tiamulin

NO

O

ON

CH3

CH3

CH3

NH2

H2NO

ONN

O2N O

O

O

OH

HSN

O

H3C

H3C H3CH3C

CH3

CH3

H2C

Chemical structures of sulfonamides and tetracyclines see page 5


Typical samples preparationSPE of veterinary drugs from fish

MN AAp MNo 305080JM Stevens et al GIT Spezial Separation 12009 16ndash17Sample pretreatment see wwwmn-netcomaAAs

c Column type CHROMABONDreg SA 3 mL 500 mg REF 730077

Column conditioning 2 x 3 mL n-hexane drying by vacuum then 3 mL 05 acetic acid in ethyl acetate do not let run the column drySample aspiration 5ndash8 mLmin then 10ndash12 min dryingColumn washing 10 mL methanolElution 5 mL 10 triethanolamine in methanolCompounds investigated sulfamerazine sulfadimidine sulfadimethoxine trimethoprim sulfadiazine chlortetracycline oxytetracycline ampicillin amoxycillin benzylpenicillin ciprofloxacin enrofloxacin norfloxacin oxolinic acid pefloxacin flumequine sarafloxacin furazolidone erythromycin spiramycin gentamicin chloram-phenicol florfenicol thiamphenicol tiamulin nalidixic acid miloxacinRecovery rates 85 to100 for spiked samples with concen-trations from 0001 to 0100 mgmL

SamplefiltrationandstorageIt is recommended to filter the prepared sample through dis-posable filters CHROMAFILregXtraPVDF-4525 REF 729219

Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871

116

x 3

2 m

mSPE of tetracyclines from musculature

MN AAp MNo 302030Courtesy of Mr Lippold Chemisches Landesuntersuchungs-amt (Chem Research Agency) Freiburg GermanySample pretreatment see wwwmn-netcomaAAs

c Column type CHROMABONDreg Tetracycline 6 mL 500 mg REF 730315

Column conditioning 1 column volume methanol 1 column volume dist water then 1 column volume EDTA-succinate buffer (see description at wwwmn-netcomaAAs) do not let run the column drySample application Slowly force or aspirate the eluate of the sample pretreatment through the columnColumn washing 2 mL dist water (removal of Cu ions) 1 mL n-hexaneElution Elute with 75 mL methanol in a 25 mL tapered flask Add 1 mL of an ethylene glycol ndash methanol mixture (22 g ethylene glycol are filled up with methanol to 100 mL) and evaporate with a rotation evaporator (max 40 degC) to dryness Fill up the residue with 01 molL McIlvain-EDTA buffer (dissolve 525 g citric acid x H2O 445 g Na2HPO4 x H2O and 93 g Titriplex III in 25 L dist water adjust with NaOH to pH 4) to 400 mLCompounds investigated tetracycline oxytetracycline chlortetracycline 100ndash500 mgkgRecovery ratestetracycline chlortetracycline ca 50ndash70 oxytetracycline ca 60ndash80

CHROMABONDreg vacuum manifold for 12 columns REF 730150

For other applications see wwwmn-netcomaAAs


AnalysisSubsequentanalysisHPLCSulfonamides

MN AAp MNo 125860 Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm

REF 76057830

0 2 4 6 8 min

1

23

4

5

6

7

Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfapyridine4 Sulfamerazine5 Sulfadimidine6 Sulfathiazole7 Sulfadimethoxine

Sulfapyridine

HNN NH2

O

OS

Sulfathiazole

SHN

O

O

SN

NH2

Sulfachloropyridazine Sulfaquinoxaline

NN HN NH2

O

OS

ClHNN

N

NH2

O

OS

Sulfadiazine R1=R2=HSulfamerazine R1 = H R2 = CH3Sulfadimidine R1 = R2 = CH3 Sulfadimethoxine

NN

HN NH2

O

OS

R2

R1 NN

HN NH2

O

OS

OCH3

H3CO

TetracyclinesMN AAp MNo 127050

Column EC 502 NUCLEOSHELLreg RP 18plus 27 μm REF 76323220

0 1 2 3 4 min

1

2

3

4

5

OH O O OHO

OH

NH2

N(CH3)2R4 R1R3

OH

R2

Peaks R1 R2 R3 R4

1 Minocycline H H H NH22 Oxytetracycline OH OH CH3 H3 Tetracycline H OH CH3 H4 Chlortetracycline H OH CH3 Cl5 Doxycycline OH H CH3 H

Detailed conditions are available online at wwwmn-netcomaAAs


Analysis Antibacterial agents

MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm

REF 76032546

8

7

65

4

3

2

1

9

10

11

0 1 2 3 4 5 6 7 8 min

Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid


MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings


Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077

Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg

30 730315

Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom

SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom

Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom

MACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com


Focus on antibiotics The global production and trading of food and feed products makes monitoring and control difficult

Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life

MN shows examples for food analysis that will help you with your work and make our food safer

Vol 3 Antibiotics in food of animal origin

Healthy animals provide healthy food

Antibiotics or antimicrobials are primarily used to treat infections caused by bacteria and other microorganisms but also they show growth promoting effects

In order to have the possibility to prevent diseases a ju-dicious use of antimicrobials is necessary since bacteria can be changed and then antibiotics are no longer effec-tive in people who need them to treat infections Antibiot-ic resistance is now a major threat to public health[WHO ndash World Health Organisation ndash httpwwwwhointmediacentrenewsreleases2014amr-reporten]

There are different classes of antimicrobials egbullPenicillinsbullAminoglycosidesbullArsenicalsbullPolypeptidesbullGlycolipidsbullTetracyclinesbullMacrolidesbullLincosamidesbullβ-LactamsbullQuinolones fluoroquinolonebullStreptograminsbullSulfonamidesbulland others

[Wikipedia ndash httpenwikipediaorg wikiList_of_antibiotics]

Most of these examples are antibiotics that were first iso-lated from a natural source Nowadays they are mostly produced synthetically and new antibiotics are usually semisynthetic modifications

Most of the antibiotics used in food animals are the

same as those used in humans

Due to the importance of antibiotic resistance as a public health problem

many governments and professional societies have reviewed existing scien-tific evidence and developed recommen-dations to limit all types of antibiotic use including use in food animals [National Anti-

microbial Resistance Monitoring System (NARMS) ndash FDA httpwwwfdagovAnimalVeterinarySafety

HealthAntimicrobialResistanceNationalAntimi-crobialResistanceMonitoringSystemdefaulthtm

ndash CDC httpwwwcdcgovnarms ndash USDA httpwwwarsusdagovsaabearnarms]





F

NO

N

NR

Norfloxacin (R = H)


H5C2

NO

NCOOH

F

N

RH



R

O

N

OO

Nalidixic acid

COOH

H5C2

H3CO

NN

Sarafloxacin

NH ndash NCOOH

F

F

O

N

Flumequine

COOH

CH3

FO

N


O

CH3

CH3OCH3

O HO

H3C

ON(CH3)2

O HO

H3C

H3CH3CH5C2 O

O

CH3

CH3

CH3OHOHHO

O

OO

O

O

O

O O

OH

N

N

H3C

H3C

H3C

H3CO

CH3

O OH

OH

CH3

H3C

HOCH3

CH3

CH3

CH3

O


O

OO

N

S

NH NH2

H3CH3C

HOO

HO

OO

N

S

NH NH2

OH

H3CH3C

O

OO

N

S

NH

H3CH3C

HO


O2N HO O

HN

OH Cl

ClS HO O

HN

OH

H3C

Cl

Cl

O OS F O

HN

OH

H3C

Cl

Cl

O O


NO

O

ON

CH3

CH3

CH3

NH2

H2NO

ONN

O2N O

O

O

OH

HSN

O

H3C

H3C H3CH3C

CH3

CH3

H2C









116

x 3

2 m










REF 76057830

0 2 4 6 8 min

1

23

4

5

6

7


Sulfapyridine

HNN NH2

O

OS

Sulfathiazole

SHN

O

O

SN

NH2


NN HN NH2

O

OS

ClHNN

N

NH2

O

OS


NN

HN NH2

O

OS

R2

R1 NN

HN NH2

O

OS

OCH3

H3CO



0 1 2 3 4 min

1

2

3

4

5

OH O O OHO

OH

NH2

N(CH3)2R4 R1R3

OH

R2

Peaks R1 R2 R3 R4






REF 76032546

8

7

65

4

3

2

1

9

10

11

0 1 2 3 4 5 6 7 8 min








30 730315



100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com





F

NO

N

NR

Norfloxacin (R = H)


H5C2

NO

NCOOH

F

N

RH



R

O

N

OO

Nalidixic acid

COOH

H5C2

H3CO

NN

Sarafloxacin

NH ndash NCOOH

F

F

O

N

Flumequine

COOH

CH3

FO

N


O

CH3

CH3OCH3

O HO

H3C

ON(CH3)2

O HO

H3C

H3CH3CH5C2 O

O

CH3

CH3

CH3OHOHHO

O

OO

O

O

O

O O

OH

N

N

H3C

H3C

H3C

H3CO

CH3

O OH

OH

CH3

H3C

HOCH3

CH3

CH3

CH3

O


O

OO

N

S

NH NH2

H3CH3C

HOO

HO

OO

N

S

NH NH2

OH

H3CH3C

O

OO

N

S

NH

H3CH3C

HO


O2N HO O

HN

OH Cl

ClS HO O

HN

OH

H3C

Cl

Cl

O OS F O

HN

OH

H3C

Cl

Cl

O O


NO

O

ON

CH3

CH3

CH3

NH2

H2NO

ONN

O2N O

O

O

OH

HSN

O

H3C

H3C H3CH3C

CH3

CH3

H2C









116

x 3

2 m










REF 76057830

0 2 4 6 8 min

1

23

4

5

6

7


Sulfapyridine

HNN NH2

O

OS

Sulfathiazole

SHN

O

O

SN

NH2


NN HN NH2

O

OS

ClHNN

N

NH2

O

OS


NN

HN NH2

O

OS

R2

R1 NN

HN NH2

O

OS

OCH3

H3CO



0 1 2 3 4 min

1

2

3

4

5

OH O O OHO

OH

NH2

N(CH3)2R4 R1R3

OH

R2

Peaks R1 R2 R3 R4






REF 76032546

8

7

65

4

3

2

1

9

10

11

0 1 2 3 4 5 6 7 8 min








30 730315



100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com








116

x 3

2 m










REF 76057830

0 2 4 6 8 min

1

23

4

5

6

7


Sulfapyridine

HNN NH2

O

OS

Sulfathiazole

SHN

O

O

SN

NH2


NN HN NH2

O

OS

ClHNN

N

NH2

O

OS


NN

HN NH2

O

OS

R2

R1 NN

HN NH2

O

OS

OCH3

H3CO



0 1 2 3 4 min

1

2

3

4

5

OH O O OHO

OH

NH2

N(CH3)2R4 R1R3

OH

R2

Peaks R1 R2 R3 R4






REF 76032546

8

7

65

4

3

2

1

9

10

11

0 1 2 3 4 5 6 7 8 min








30 730315



100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com




REF 76057830

0 2 4 6 8 min

1

23

4

5

6

7


Sulfapyridine

HNN NH2

O

OS

Sulfathiazole

SHN

O

O

SN

NH2


NN HN NH2

O

OS

ClHNN

N

NH2

O

OS


NN

HN NH2

O

OS

R2

R1 NN

HN NH2

O

OS

OCH3

H3CO



0 1 2 3 4 min

1

2

3

4

5

OH O O OHO

OH

NH2

N(CH3)2R4 R1R3

OH

R2

Peaks R1 R2 R3 R4






REF 76032546

8

7

65

4

3

2

1

9

10

11

0 1 2 3 4 5 6 7 8 min








30 730315



100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com




REF 76032546

8

7

65

4

3

2

1

9

10

11

0 1 2 3 4 5 6 7 8 min








30 730315



100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com




30 730315



100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0119

Foc

us F

lyer

Foo

d Vo

l 3

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

Bob

o s

hock

ndash fo

tolia

com

COMATOGAP -...

Documents

Transcript of COMATOGAP -...