Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University...
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Transcript of Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University...
![Page 1: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/1.jpg)
Columbia University
Amy Hale
Won Yung Choi
Yaniv Eyny
Johannes Schwaninger
Princeton University
Barry Jacobs, Ph.D.
Tripp Stewart
![Page 2: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/2.jpg)
A photo-emitter -detector reports each head entry into the food compartment
Movement of the rat is detected as its infrared body heat crosses compartments of a mounted lens.
![Page 3: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/3.jpg)
Sprague-Dawley rats received 16 days of drug-free training (28 trials/day).
On day 17, rats were pretreated with vehicle, D1 antagonist SCH23390 or D2 antagonist raclopride.
Pellets drop, on average, every 70 sec.
Each pellet delivery is preceded by a 400 ms, 78 dB sound.
28 trials are presented during each session
![Page 4: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/4.jpg)
Drug freeSubject 31
Seconds (relative to CS onset)
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Trial
-16 0 10
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D1 antagonistSCH 23390
Subject 31
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-16 0 10
Trial
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Selective D2 dopamine receptor blockade
VEH, Day 16
-16 0
Trial
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Subject 7
RAC 0.4 (Day 17)
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Time (sec relative to food delivery)
Trial
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from Horvitz and Eyny 2000
![Page 7: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/7.jpg)
Dopamine is needed to execute internally-generated responses,
but not for responses to a well-acquired conditioned stimulus
![Page 8: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/8.jpg)
Saline, Rat 41
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Time (sec)
Tri
al
Day 3 of training
SCH23390 0.16 mg/kg, Rat 32
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Time (sec)
Tri
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D1 receptor blockade increases the frequency of missed trials early but not late in training
Missed trials (proportion of
total)
Days of training
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Veh0.04 SCH0.08 SCH0.16 SCH
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But is necessary to execute responses to a conditioned stimulus during early stages of learning
Dopamine is not needed to execute responses to a well-acquired conditioned stimulus
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Test
CS-Food sessions
3-3 group
3-7 groupTest
7-7 group
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Training Sessions/Days
Lat
ency
(se
c)
Veh
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Test
CS-Food sessions
3-3 group
3-7 groupTest
7-7 group
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Training Sessions/Days
Lat
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Veh
SCH 0.16
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CORTEX
Motor planning
(anterior frontal lobe)Motor strip
Striatum
DA neurons
Thalamus Gl Pall
sensory
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Dopamine neurons respond to salient auditory and visual stimuli
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click click signals reward
click click signals reward
1
43
2
Dopamine neuronal response to a click is enhanced when the click signals reward delivery
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TONE ONSET 1 2 3 4 5
TONE END 7 8 9 10 11 12 13 14 15
He
ad
-in
(p
rop
ort
ion
of
tria
ls)
Unpaired
Veh
D1 receptor blockade disrupts acquisition of head-entry response to food cue
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He
ad-i
n (
pro
po
rtio
n o
f tr
ials
) Unpaired
Veh
.08 SCH
.16 SCH
D1 receptor blockade disrupts acquisition of head-entry response to food cue
![Page 18: Columbia University Amy Hale Won Yung Choi Yaniv Eyny Johannes Schwaninger Princeton University Barry Jacobs, Ph.D. Tripp Stewart.](https://reader035.fdocuments.net/reader035/viewer/2022062517/56649f165503460f94c2b855/html5/thumbnails/18.jpg)
14 15
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-10 -9 -8 -7 -6 -5 -4 -3 -2 -1
TONE ONSET 1 2 3 4 5
TONE END 7 8 9 10 11 12 13
He
ad
-in
(p
rop
ort
ion
of
tria
ls)
Unpaired
Veh
.2 Rac
.4 Rac
D2 receptor blockade promotes acquisition of head-entry response to food cue
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Dopamine plays a critical role in the acquisition of responses to new environmental stimuli, and D1 and D2 receptors appear to play opposing roles in this process.
Somehow, learned responses become dopamine-independent with overtraining.
00.10.20.30.40.50.60.70.80.9
1
3 17
Veh
0.04 SCH0.08 SCH0.16 SCH
Dopamine transmission is needed for the execution of internally generated responses,
but not for the execution of responses to well-acquired conditioned stimuli
0
4
8
12
16
20
24
28
-16 0 10
Tria
l
Thalamus
Striatum Gl Pall
cortex