Cm3 safety in the lab
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SAFETY in the Clinical Laboratory
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Psalm 24: 4-5
He who has clean hands and a pure heart, who does not lift up his soul to what is false and does not swear deceitfully.
He will receive blessing from the Lordand righteousness from the God of His salvation.
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OUTLINE
• Safety Hazards
• Sources of Hazards
• How to deal with hazards
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SAFETY HAZARDSBiological
SharpChemical
RadioactiveElectrical
Fire/explosivePhysical
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Biological HazardsSource• Specimen• Fomites• Patients
Possible infection• Bacterial• Fungal• Viral• parasitic
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Chain of Infection
Host
TransmissionSource
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Universal Precautions (UP)
All patients are considered to be possible carriers of bloodborne
pathogensIt excluded urine and other body fluids as potentially infectious.
By CDC and OSHA
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Body Substance Isolation (BSI)
All body fluids and moist
body substances are
considered to be
potentially infectious.
Hand washing is NOT recommended after the removal of gloves.
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Standard Precautions (SP)
Treat all biological
specimens as potentially
infectious.
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• Latex
• Powder free nitrile
• Cotton
• Lightweight cotton
• Cut-resistant
• Finger cots
• Heavy duty neoprene
• Polyethylene
• Rubber
SP
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What is the disinfectant of choice for
bloodborne pathogens?
SODIUM HYPOCHLORITE (1:10)
How are biological wastes disposed?
Incineration, autoclaving or pickup by
certified hazardous waste company
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Sharp hazards
Possible Injury
• Cuts, punctures
• Bloodborne pathogen exposure
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Chemical Hazards
Source
• Preservatives
• Reagents
Possible Injury
• Exposure to
toxic,
carcinogenic, or
caustic agents
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• Chemical spills
• Chemical handling
• Chemical Hygiene Plan (CHP)
• Chemical labeling
• Material Safety Data Sheet (MSDS)
Chemical Hazards
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Radioactive hazards
The degree of
radiation
exposure is
related to the
combination of
time, distance
and shielding.
Source
• Equipment
• Radioisotopes
Possible injury
• Radiation
exposure
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Electrical Hazards
Sources
• Ungrounded or
wet equipment
• Frayed cords
Possible injury
• Burns
• shock
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Fire hazards
Sources
• Bunsen burners
• Organic chemicals
(*flash point)
– Combustible
– Flammable
Possible injury
• Burns
• Dismemberment
ECAR
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Using the fire extinguisher
• PULL...Pull the pin. (lock latch, pressing a puncture lever, or other motion)
• AIM...Aim low, pointing the extinguisher nozzle at the base of the fire.
• SQUEEZE...Squeeze the handle.
• SWEEP...Sweep from side to side at the base of the fire until it appears to be out. Watch the fire area in case fire breaks out again, and repeat use of the extinguisher if necessary.
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Physical hazards
• Running
• Wet floors
• Proper body movements
• Long Hair
• Jewelry
• Working area
• Closed shoes