Audit of the Drug Enforcement Administration's Aviation Operations ...
Clonazolam The Drug Enforcement Administration's Special ...Clonazolam The Drug Enforcement...
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Clonazolam The Drug Enforcement Administration's Special Testing and Research Laboratory generated this monograph using structurally confirmed reference material. Latest Revision: 10/18/16 SWGDRUG.org/monographs.htm Page 1 of 5 N N N C H 3 N N O O Cl 1. GENERAL INFORMATION IUPAC Name: 6-(2-chlorophenyl)-1-methyl-8-nitro-4 H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine CAS#: 33887-02-4 Synonyms: Clonitrazolam Source: DEA Reference Material Collection Appearance: Yellow powder UV max (nm): Not Determined 2. CHEMICAL AND PHYSICAL DATA 2.1 CHEMICAL DATA Form Chemical Formula Molecular Weight Melting Point ( o C) Base C 17 H 12 ClN 5 O 2 353 Not Determined
Transcript of Clonazolam The Drug Enforcement Administration's Special ...Clonazolam The Drug Enforcement...
ClonazolamThe Drug Enforcement Administration's Special Testing and Research Laboratory
generated this monograph using structurally confirmed reference material.
Latest Revision: 10/18/16 SWGDRUG.org/monographs.htm Page 1 of 5
N N
N
CH3
N
NO
O
Cl
1. GENERAL INFORMATION
IUPAC Name: 6-(2-chlorophenyl)-1-methyl-8-nitro-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine
CAS#: 33887-02-4
Synonyms: Clonitrazolam
Source: DEA Reference Material Collection
Appearance: Yellow powder
UVmax(nm): Not Determined
2. CHEMICAL AND PHYSICAL DATA
2.1 CHEMICAL DATA
Form Chemical Formula Molecular Weight Melting Point (oC)
Base C17H12ClN5O2 353 Not Determined
ClonazolamThe Drug Enforcement Administration's Special Testing and Research Laboratory
generated this monograph using structurally confirmed reference material.
8.5
Latest Revision: 10/18/16 SWGDRUG.org/monographs.htm Page 2 of 5
3. QUALITATIVE DATA
3.1 NUCLEAR MAGNETIC RESONANCE
Sample Preparation: Dilute analyte to ~15 mg/mL in deuterated methanol containing TMS for 0 ppmreference and dimethylsulfone as quantitative internal standard.
Instrument: 400 MHz NMR spectrometerParameters: Spectral width: at least containing -3 ppm through 13 ppm
Pulse angle: 90o
Delay between pulses: 45 seconds1H NMR: Clonazolam Lot RM-151119-01; CD3OD; 400MHz
8 7 6 5 4 3 2 1 0
31112121
TMS
dimethylsulfone
from CD3OD from CD3OD
8.0 7.5
12121
5.40 5.35
1
4.425 4.375
1
2.675
3
ClonazolamThe Drug Enforcement Administration's Special Testing and Research Laboratory
generated this monograph using structurally confirmed reference material.
Latest Revision: 10/18/16 SWGDRUG.org/monographs.htm Page 3 of 5
3.2 GAS CHROMATOGRAPHY/MASS SPECTROMETRY
Sample Preparation: Dilute analyte ~3 mg/mL in methanol.
Instrument: Agilent gas chromatograph operated in split mode with MS detectorColumn: DB-5 MS (or equivalent); 30m x 0.25 mm x 0.25 µmCarrier Gas: Helium at 1 mL/minTemperatures: Injector: 280oC
MSD transfer line: 280oCMS Source: 250oCMS Quad: 150oCOven program:
1) 100oC initial temperature for 1.0 min2) Ramp to 280oC at 12 oC/min3) Hold final temperature for 9.0 min
Injection Parameters: Split Ratio = 25:1, 1 µL injectedMS Parameters: Mass scan range: 30-550 amu
Threshold: 150Tune file: stune.uAcquisition mode: scan
Retention Time: 17.99 minEI Mass Spectrum: Clonazolam Lot RM-151119-01
m/z340320300280260240220200180160140120100806040
0
8
16
24
32
40
48
56
64
72
80
88
96
5151515151515151515151515151515151515151515163636363636363636363636363636363636363636363
75757575757575757575757575757575757575757575
7676767676767676767676767676767676767676767689898989898989898989898989898989898989898989
101101101101101101101101101101101101101101101101101101101101101101
102102102102102102102102102102102102102102102102102102102102102102
111111111111111111111111111111111111111111111111111111111111111111137137137137137137137137137137137137137137137137137137137137137137
142142142142142142142142142142142142142142142142142142142142142142 149149149149149149149149149149149149149149149149149149149149149149150150150150150150150150150150150150150150150150150150150150150150
176176176176176176176176176176176176176176176176176176176176176176177177177177177177177177177177177177177177177177177177177177177177
202202202202202202202202202202202202202202202202202202202202202202
203203203203203203203203203203203203203203203203203203203203203203
204204204204204204204204204204204204204204204204204204204204204204
238238238238238238238238238238238238238238238238238238238238238238
249249249249249249249249249249249249249249249249249249249249249249
278278278278278278278278278278278278278278278278278278278278278278
324324324324324324324324324324324324324324324324324324324324324324
326326326326326326326326326326326326326326326326326326326326326326
353353353353353353353353353353353353353353353353353353353353353353
EI+
ClonazolamThe Drug Enforcement Administration's Special Testing and Research Laboratory
generated this monograph using structurally confirmed reference material.
1700 1600 1500 1400 1300 1200 1100 1000 900 800 700 Wavenumber (cm-1) Latest Revision: 10/18/16 SWGDRUG.org/monographs.htm Page 4 of 5
3.3 INFRARED SPECTROSCOPY (FTIR)
Insrument: FTIR with diamond ATR attachment (1 bounce)Scan Parameters: Number of scans: 32
Number of background scans: 32Resolution: 4 cm-1
Sample gain: 1Aperture: 150
FTIR ATR (Diamond 1 Bounce): Clonazolam Lot RM-151119-01
Wavenumber (cm-1)3500 3000 2500 2000 1500
%Tr
ansm
ittan
ce
30
40
50
60
70
1612161216121612161216121612161216121612161216121612161216121612161216121612161216121612
1531153115311531153115311531153115311531153115311531153115311531153115311531153115311531
1519151915191519151915191519151915191519151915191519151915191519151915191519151915191519
14861486148614861486148614861486148614861486148614861486148614861486148614861486148614861428142814281428142814281428142814281428142814281428142814281428142814281428142814281428
1350135013501350135013501350135013501350135013501350135013501350135013501350135013501350
1321132113211321132113211321132113211321132113211321132113211321132113211321132113211321
10991099109910991099109910991099109910991099109910991099109910991099109910991099109910991058105810581058105810581058105810581058105810581058105810581058105810581058105810581058 1038
103810381038103810381038103810381038103810381038103810381038103810381038103810381038
999999999999999999999999999999999999999999999999999999999999999999
970970970970970970970970970970970970970970970970970970970970970970
878878878878878878878878878878878878878878878878878878878878878878
837837837837837837837837837837837837837837837837837837837837837837
776776776776776776776776776776776776776776776776776776776776776776
742742742742742742742742742742742742742742742742742742742742742742
726726726726726726726726726726726726726726726726726726726726726726
669669669669669669669669669669669669669669669669669669669669669669542542542542542542542542542542542542542542542542542542542542542542520520520520520520520520520520520520520520520520520520520520520520
%Tr
ansm
ittan
ce
30
40
50
60
70
1612161216121612161216121612161216121612161216121612161216121612161216121612161216121612
1542154215421542154215421542154215421542154215421542154215421542154215421542154215421542
1531153115311531153115311531153115311531153115311531153115311531153115311531153115311531
1519151915191519151915191519151915191519151915191519151915191519151915191519151915191519
1486148614861486148614861486148614861486148614861486148614861486148614861486148614861486
1470147014701470147014701470147014701470147014701470147014701470147014701470147014701470
1428142814281428142814281428142814281428142814281428142814281428142814281428142814281428
1402140214021402140214021402140214021402140214021402140214021402140214021402140214021402
1374137413741374137413741374137413741374137413741374137413741374137413741374137413741374
1350135013501350135013501350135013501350135013501350135013501350135013501350135013501350
1321132113211321132113211321132113211321132113211321132113211321132113211321132113211321
1250125012501250125012501250125012501250125012501250125012501250125012501250125012501250
1201120112011201120112011201120112011201120112011201120112011201120112011201120112011201
10991099109910991099109910991099109910991099109910991099109910991099109910991099109910991058105810581058105810581058105810581058105810581058105810581058105810581058105810581058 1038
103810381038103810381038103810381038103810381038103810381038103810381038103810381038
999999999999999999999999999999999999999999999999999999999999999999
970970970970970970970970970970970970970970970970970970970970970970
914914914914914914914914914914914914914914914914914914914914914914878878878878878878878878878878878878878878878878878878878878878878
837837837837837837837837837837837837837837837837837837837837837837
808808808808808808808808808808808808808808808808808808808808808808
776776776776776776776776776776776776776776776776776776776776776776
742742742742742742742742742742742742742742742742742742742742742742
726726726726726726726726726726726726726726726726726726726726726726
703703703703703703703703703703703703703703703703703703703703703703
669669669669669669669669669669669669669669669669669669669669669669
638638638638638638638638638638638638638638638638638638638638638638
550550550550550550550550550550550550550550550550550550550550550550 542
542542542542542542542542542542542542542542542542542542542542542520520520520520520520520520520520520520520520520520520520520520520
ClonazolamThe Drug Enforcement Administration's Special Testing and Research Laboratory
generated this monograph using structurally confirmed reference material.
Latest Revision: 10/18/16 SWGDRUG.org/monographs.htm Page 5 of 5
http://forendex.southernforensic.org/index.php/detail/index/1323
http://en.wikipedia.org/wiki/Clonazolam
4. ADDITIONAL RESOURCES
Forendex
Wikipedia
