Test Name Test Code Technique Specimen TAT/Reported on

Anti-Cyclic Citrullinated Peptide - Anti CCP

RIM10196Chemiluminescence

Microparticle Immunoassay (CMIA)

3 ml (1.5 ml Minimum) Serum from 1 SST (Gel

Barrier Tube) drawn between 8- 10 a.m. In a

fasting condition

Same Day by 7:00 p.m.

Anti-Nuclear Antibody - ANA, ELISA

RIM10301ELISA (Enzyme Linked

Immuno sorbent Assay)

2 ml. (1 ml. Minimum) Serum from 1 SST (Gel

Barrier Tube).

Next Working Day by 7:00 p.m.

Anti-Nuclear Antibody - ANA, IFA

RIM10187

IFA (Immunofluorescence Assay)

Anti-Neutrophilic Cytoplasmic Antibodies - ANCA

RIM101915th working by 7:00

p.m.

Smooth Muscle Antibody (ASMA), IFA

RIM10251Next Working Day by

7:00 p.m.

ASLO (Antistrepto Lysin -O) RBC10074

Latex Agglutination

Same Day by 7:00 p.m.

CRP (C-Reactive Protein)-Qualitative

RBC10084

Rheumatoid Factor - RF Factor, Qualitative

RSR10008 Slide Agglutination

Rheumatoid Factor -RF Factor, (Quantitative)

RBC10083 Nephelometry

Acetylcholine Receptor Auto antibodies

RIM10242Radio Immunoassay

(RIA5th working day by

7:00 p.m.

Anti-Soluble Liver Antigen (SLA) RIM10305ELISA (Enzyme Linked

Immuno sorbent Assay)4th Working Day by

7:00 p.m.

Anti-Mitochondrial Antibody - AMA, IFA

RIM10218

IFA (Immunofluorescence Assay)

Next Working Day by 7:00 p.m.

LKM Antibody in Dilutions, IFA (Liver Kidney Microsomal Antibody in Dilutions, IFA)

RIM102505th working day by

7:00 p.m.

ds DNA antibody (Reflex to end titer for all positive cases)

RIM10207Next Working Day by

7:00 p.m.Anti -ds DNA Antibody, ELISA RIM10208

ELISA (Enzyme Linked Immuno sorbent Assay)

ENA (Screen)Panel Qualitative (Extractable Nuclear Antigen Screen Panel Qualitative)

RP10030 LIA (LINE Immunoassay)3 ml (1.5 ml. Minimum) Serum from 2 SSTs (Gel

Barrier Tube).

4th Working Day by 7:00 p.m.

HLA B-27, Disease Association PCR SMO10079 PCR

5 - 6 ml EDTA anticoagulated Whole

blood in 2 Lavender top tubes.

3rd working day if received before

1400 hrs.

HLA B-27 (Flowcytometry) SFC10056 Flow Cytometry3 ml. (2 ml. Minimum) Whole Blood in 1 EDTA

(Lavender top) tube

Next Working Day if received before

1400 Hrs

TTG Antibody IgA, Tissue Transglutaminase IgA Antibody

RIM10239ELISA (Enzyme Linked

Immuno sorbent Assay)

2 ml. (1 ml. Minimum) Serum from 1 SST (Gel

Barrier Tube).

Next Working Day by 7:00 p.m.

TEST INFORMATION

Regd. Office & Main Lab .: 3- Factory Road , Adj. Safdarjung Hospital, New Delhi - 29

laboratories

DISCOVERDIAGNOSEDEFEND

Clinical Testing for Autoimmune Disorders

India’s Leading Super Specialized Laboratory Network

Anti CCP (Citrullinated Cyclic Peptide antibodies)

ASMA (Anti-Smooth muscle antibodies) by Immunofluorescence

Rheumatoid Factor Testing

Acetylcholine Receptor Antibodies

HLA B27 (Human Leukocyte antigen)

• Aids in diagnosis of patients with unexplained joint inflammation, especially in Seronegative RF (Rheumatoid Factor)

• Provides differential diagnosis of Rheumatoid arthritis from other forms of joint disease

• Initial Screening test and highly sensitive • Helps to evaluate a person for autoimmune disorders that affect many tissues

and organs throughout the body

• ANCA tests are used to diagnose and monitor inflammatory activity in the primary systemic small vessel vasculitis

• May be useful for monitoring treatment response in patients with WG (systemic or organ-limited disease); increasing titer suggests relapse of disease, while a decreasing titer suggests successful treatment

• Consist of fewer extractable antigens or recombinant antigens that can be detected by solid phase technique

• More specific than IFA as specific Ag can be used to detect specific disease• Potential for quantification

• M2 antibody is the most commonly detected and clinically significant mitochondrial antibody

• Helps in detection of antibodies in patients with PBC (Primary Biliary Cirrhosis)

• Specific marker for Autoimmune Hepatitis Type-1 (50-80%) & PBC (20%)

• Can also be found in Drug induced hepatitis, Alcohol induced liver cirrhosis, Primary Sclerosing cholangitis, Breast & Ovarian Cancer and 2% of apparently healthy persons

• Can be transiently detected in other viral infections including Hepatitis B, C & Infectious Mononucleosis

• Absence of SMA indicates non- autoimmune forms of Chronic Hepatitis

• Approximately 85% of patients with rheumatoid arthritis have detectable RF• It may also be seen in 1-5% of healthy people and in other with medical conditions

like Sjogren’s syndrome and Systemic Lupus Erythematosus (SLE), Tuberculosis, syphilis etc

• Majority of MG patients demonstrate elevated serum levels of acetylcholine receptor (AChR) antibodies

• Myasthenia gravis has been confirmed by the detection of anti-AChR antibody in 85-90% of the patients with generalized disease and in 75% of cases with restricted ocular myasthenia

• Used for the evaluation of seronegative spondyloarthropathies• Aids in diagnosis of Ankylosing Spondylitis & Reiter’s syndrome• Single platform Technology, Dual Laser & 6 color immunofluorescence technique

is used to identify & report percentages of HLA B27 positive cell population with utmost precision

More Than a Decade of Experience in delivering Pathological Solution India’s Leading Super Specialized Laboratory Network

ANA (Antinuclear Antibody) by Immunofluorescence

Anti-Neutrophilic Cytoplasmic Antibodies – ANCA

ELISA: Poly or Monospecific

• Multiplexed immunoassays and uses recombinant antigens• Easy to perform, rapid • Comparing the color intensities• Highly sensitive, SSA/Ro + can be detected• Automation is possible

LIA (Line Blot)

AMA (Anti-Mitochondrial Antibody) by Immunofluorescence

Also available• SLA/LP (Anti-Soluble Liver Antigen/Liver-Pancreas)

• LKM (Liver Kidney Microsomal Antibody)

• Anti-ds DNA

• Anti-TTG (Anti-Tissue Transglutaminase Antibody)

• Immunoglobulins (Quantitative & Qualitative)

• CRP (C-Reactive Protein) Testing

• ASO/ASLO (Anti-Streptolysin O)