Clinical significance of circulatory chlamydial heat shock protein-60 antibodies in...
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POSTER PRESENTATION Open Access Clinical significance of circulatory chlamydial heat shock protein-60 antibodies in spondyloarthropathy patients Praveen Kumar 1 , Darshan Singh Bhakuni 2 , Sangita Rastogi 1* From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014) Chennai, India. 30 January - 1 February 2014 Background Chlamydia trachomatis is a multifaceted, asymptomatic, most prevalent sexually transmitted pathogen world- wide. Due to lack of specific diagnostic methods for C. trachomatis in Spondyloarthropathy (SpA) patients, this pathogen is under diagnosed and under estimated in various countries including India. We aimed to estimate circulatory antibodies to chlamydial heat shock protein- 60 (chsp60) IgG/ anti C. trachomatis IgA and evaluate their clinical significance in SpA patients. Methods In this case control study, serum from 60 arthritic patients comprising of Reactive Arthritis (ReA), undifferentiated SpA, Rheumatoid Arthritis (RA) and Osteoarthritis (OA) was collected for detection of anti chsp60 IgG and anti C. trachomatis IgA antibodies by using commercial ELISA kits. Cut-off indices (COI) of infected SpA patients were correlated with clinical data. Results Overall, 12/ 30 (40.0%) SpA patients, viz.: ReA/uSpA, were found positive for either circulatory chsp60 IgG or anti C. trachomatis IgA antibody (p value < 0.009 versus RA/ OA). 23.3% (7/ 30) SpA patients and 3.3 % (1/30) RA/OA control patients were found to be positive for cir- culatory chsp60 IgG antibodies. 16.6% (5/ 30) patients found positive for anti C. trachomatis IgA antibodies; however, none of the controls was infected. COI, age and disease duration in chsp60-positive SpA patients showed significant difference in median values (Kruskal-Wallis test; p value < 0.0001) while there was no such relation to anti C. trachomatis IgA. Conclusion Overall findings indicate an active/chronic C. trachomatis infection in SpA patients. The study also suggests useful- ness of chsp60 serology to facilitate better clinical manage- ment of patients. Authors’ details 1 Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital campus, New Delhi, India. 2 Department of Rheumatology and Clinical Immunology, Army Hospital (Research & Referral), New Delhi, India. Published: 27 May 2014 doi:10.1186/1471-2334-14-S3-P59 Cite this article as: Kumar et al.: Clinical significance of circulatory chlamydial heat shock protein-60 antibodies in spondyloarthropathy patients. BMC Infectious Diseases 2014 14(Suppl 3):P59. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital campus, New Delhi, India Full list of author information is available at the end of the article Kumar et al. BMC Infectious Diseases 2014, 14(Suppl 3):P59 http://www.biomedcentral.com/1471-2334/14/S3/P59 © 2014 Kumar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Transcript of Clinical significance of circulatory chlamydial heat shock protein-60 antibodies in...
POSTER PRESENTATION Open Access
Clinical significance of circulatory chlamydialheat shock protein-60 antibodies inspondyloarthropathy patientsPraveen Kumar1, Darshan Singh Bhakuni2, Sangita Rastogi1*
From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)Chennai, India. 30 January - 1 February 2014
BackgroundChlamydia trachomatis is a multifaceted, asymptomatic,most prevalent sexually transmitted pathogen world-wide. Due to lack of specific diagnostic methods forC. trachomatis in Spondyloarthropathy (SpA) patients,this pathogen is under diagnosed and under estimated invarious countries including India. We aimed to estimatecirculatory antibodies to chlamydial heat shock protein-60 (chsp60) IgG/ anti C. trachomatis IgA and evaluatetheir clinical significance in SpA patients.
MethodsIn this case control study, serum from 60 arthritic patientscomprising of Reactive Arthritis (ReA), undifferentiatedSpA, Rheumatoid Arthritis (RA) and Osteoarthritis (OA)was collected for detection of anti chsp60 IgG and antiC. trachomatis IgA antibodies by using commercial ELISAkits. Cut-off indices (COI) of infected SpA patients werecorrelated with clinical data.
ResultsOverall, 12/ 30 (40.0%) SpA patients, viz.: ReA/uSpA,were found positive for either circulatory chsp60 IgG oranti C. trachomatis IgA antibody (p value < 0.009 versusRA/ OA). 23.3% (7/ 30) SpA patients and 3.3 % (1/30)RA/OA control patients were found to be positive for cir-culatory chsp60 IgG antibodies. 16.6% (5/ 30) patientsfound positive for anti C. trachomatis IgA antibodies;however, none of the controls was infected. COI, age anddisease duration in chsp60-positive SpA patients showedsignificant difference in median values (Kruskal-Wallis
test; p value < 0.0001) while there was no such relation toanti C. trachomatis IgA.
ConclusionOverall findings indicate an active/chronic C. trachomatisinfection in SpA patients. The study also suggests useful-ness of chsp60 serology to facilitate better clinical manage-ment of patients.
Authors’ details1Microbiology Laboratory, National Institute of Pathology (ICMR), SafdarjungHospital campus, New Delhi, India. 2Department of Rheumatology andClinical Immunology, Army Hospital (Research & Referral), New Delhi, India.
Published: 27 May 2014
doi:10.1186/1471-2334-14-S3-P59Cite this article as: Kumar et al.: Clinical significance of circulatorychlamydial heat shock protein-60 antibodies in spondyloarthropathypatients. BMC Infectious Diseases 2014 14(Suppl 3):P59.
Submit your next manuscript to BioMed Centraland take full advantage of:
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at www.biomedcentral.com/submit
* Correspondence: [email protected] Laboratory, National Institute of Pathology (ICMR), SafdarjungHospital campus, New Delhi, IndiaFull list of author information is available at the end of the article
Kumar et al. BMC Infectious Diseases 2014, 14(Suppl 3):P59http://www.biomedcentral.com/1471-2334/14/S3/P59
© 2014 Kumar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
