(classical) genetics

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(classical) genetics causality phenotype DNA ‘reverse’ genetics

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(classical) genetics. causality. DNA. phenotype. ‘reverse’ genetics. T. C. The ‘gapped duplex’ method of in vitro mutagenesis. Gapped duplex method with phenotypic selection. Target : replace Trp by Phe Screening aid : Mnl I cleavage Mismatch primer : …GCCCT G GGCT TC GGTGGCA…. - PowerPoint PPT Presentation

Transcript of (classical) genetics

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(classical) genetics

causalityphenotypeDNA

‘reverse’ genetics

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TC

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The ‘gapped duplex’ method of in vitro mutagenesis

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Gapped duplex method with phenotypic selection

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Creating or removing a restriction recognition site as an aid in screening for site-specific mutants

Target : replace Trp by Phe

Screening aid : MnlI cleavage

Mismatch primer : …GCCCTGGGCTTCGGTGGCA…

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Codewoord tabel voor ‘reverse translation’

Leu = 1T2 Arg = 3G4 Ser = 567

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