Citrus Pathology PLP 5115C Fall 2019 · anatomy, plant physiology, genetics, molecular biology,...
Transcript of Citrus Pathology PLP 5115C Fall 2019 · anatomy, plant physiology, genetics, molecular biology,...
Citrus Pathology PLP 5115C Fall 2019
Meeting times: Mondays at 9:35 am to 11:30 am and Fridays at 12:50 to 1:40Instructor: Dr. Nabil KillinyOffice: Building 7103, Room 5, Citrus Research and Education CenterOffice hours: Drop-in office hours anytime, or email anytime.
Reading Materials Nothing assigned for now! Florida Citrus Diagnostic guide Compendium of Citrus Diseases Plant Pathology - Agrios Essential Plant Pathology- Schumann and D’Arcy Plant Virology – Hull Plant Bacteriology – Kado
Concepts of Plant Pathology What is plant pathology? Pathology: the study of the nature of disease or any
abnormal condition in an organism. Plant Pathology is “1. the study of living organisms and the
environmental conditions that cause disease in plants 2. the mechanisms by which these factors produce disease in
plants , 3. the interactions between disease-causing agentsand the diseased plant, and 4. the methods of preventing orcontrolling disease and alleviating the damage it causes”
What do Plant Pathologist Study?
Diseases caused by fungi, bacteria, mycoplasmas, parasitic higher plants, viruses, viroids, nematodes and protozoa
Also plant disorders caused by excess, imbalance, or lack of physical or chemical factors (e.g. moisture, temperature and nutrients)
But not plant damage caused by insects, humans or animals
Plant Pathology uses basic knowledgeof: Botany, mycology, bacteriology, virology, nematology, plant
anatomy, plant physiology, genetics, molecular biology,genetic engineering, biochemistry, horticulture, tissueculture, soil science, forestry, chemistry, physics,meteorology, and other branches of science.
Uses advances of other sciences to solve plant pathologicalproblems.
Plant pathology attempts to increase our knowledge of thecauses and development of plant diseases
Practical goal is: to save produce that is destroyed by plantdiseases to make it available to feed and cloth the world.Done by control and management of diseases.
Concept of Disease in Plants What is a healthy plant? When it can carry out its physiological functions to the best
of its genetic potential Functions include what? normal cell division, differentiation, development;
absorption of water and minerals from the soil and translocation throughout the plant; photosynthesis and translocation of the products to areas of utilization or storage; metabolism of synthesized compounds; reproduction; and storage of food supplies for overwintering or reproduction.
Plant disease (one definition): whenever plants are disturbedby pathogens or by certain environmental conditions andone or more functions are interfered with beyond a certaindeviation from the normal
The primary cause of disease are either pathogenic livingorganisms (pathogens) or factors in the environment.
Definition of symptoms: Evidence of the reaction of theplant to the pathogen or materials that the pathogenproduces (or environmental factors). Macroscopically ormicroscopically.
Types of Function Interference Root rots: root infection Vascular wilts and cankers: interference with the vascular
system Leaf spots, blights and mosaics: infection of foliage Viral, phytoplasma and systemic bacterial infections:
interferes with translocation of photosynthetic products Bacterial and fungal blights: interferes with flowers Fruit rots: causes reduce reproduction or storage of reserve
foods for the new plant
Hyperplasia or hypertrophy: more cells produced or largercells produced resulting in nonfunctioning or abnormal cellsor abnormal cell growth; often diverting food materials fromthe rest of the plant
In all cases of disease the physiology of the plant is affected Now let’s define disease: The malfunctioning of host cells and tissues that results from
their continuous irritation by a pathogenic agent orenvironmental factor and leads to the development ofsymptoms
Is disease a condition? Yes, a condition involving abnormal changes in the form,
physiology, integrity, or behavior of the plant that may resultin the impairment or death of the plant or its parts.
How do pathogens cause disease in plants? 1. weaken the plant by absorbing food from the plant, 2.
killing or disturbing the metabolism of the cells via toxins,enzymes or growth regulating substances, 3. blocking thetransportation of food, minerals, and water, 4. consumingthe contents of the host cells
Classification of Plant Diseases I. Infectious or biotic plant diseases (type of pathogen)
caused by 1. fungi, 2. prokaryotes (bacteria orphytoplasmas), 3. parasitic higher plants, 4. viruses andviroids, 5. nematodes
II. Disease symptom: root rot, canker, mosaic, foliage, leafspot, wilt, fruit disease, post harvest, etc.
III. Field crop disease, fruit tree disease, forest disease, turfdisease, ornamental disease, etc.
Question to class: Which is the most useful?
History of Plant Diseases
Begins with fungal diseases OldTestament lists blasting and mildews Greek philosopher Theophratus (370- 286 B.C.) first to
study and write about diseases of trees, cereals and legumes. Romans: created a special rust god, Rubigo, to protect them
from cereal rusts. Each spring celebrated Robigalia 1729 Micheli: His Nova plantarum genera (1729) was
a major step in the knowledge of fungi. 1st to point out thatfungi have reproductive bodies (spores) (dust particles takenfrom a fungus reproduced the same kind of fungus
1755 Tillet: black dust from bunted wheat to seed fromhealthy wheat and produced bunt in the plants.
1807 Prevost: bunt disease caused by a fungus Debary 1853: smut and rust fungi are the causes not the
results of plant diseases. Remember he was a microscopist Brefield 1875, 1883, 1912: introduced techniques for
growing microorganisms in pure cultures. Assisted by theaccomplishments of Koch, Petri and others
1878 Downy mildew of grape was introduced into Europeform the U.S. Spread rapidly. Millardet noticed that vinessprayed with copper sulfate and lime to deter pilferersretained their leaves during the season. “Bordeaux mixture”
1882 Ward: coffee rust in Ceylon warned againstmonoculture of crops
Historical Perspectives withDifferent Causal Agents
Bacteria in Plant Disease 1876 Pasteur and Koch proved anthrax was incited by a
bacterium (Anthrax is an infectious disease of both humansand other animals caused by bacteria called Bacillusanthracis. Infection in humans most often involves the skin,the gastrointestinal tract, or the lungs).
E. F. Smith 1895: bacterial diseases of plants; bacterial wilts ofcucurbits, ornamentals, crucifers. Established bacteria asphytopathogens
1977 Chilton: Crown gall bacterium transforms normal plantcells into tumor cells (anyone know the causal agent?)
1972 Windsor and Black: Rickettsia-like organisms in thephloem of clover with clover club leaf.
1973 and after: Fastidious bacteria in the xylem of Pierce’sdisease of grapes, phony peach, and others (who cultured it?)
Nematodes in Plant Disease 1743 Needham within wheat galls 1913-1932: Cobb series of studies on plant parasitic
nematodes
Viruses in Plant Diseases 1886 Mayer: tobacco mosaic: juice from plants was
infectious. “contagium fluidium” 1929: Holmes: local lesions could be used to measure the
number of virus present in plant sap prep. 1935 Stanley: precipated the crystalline protein from
infected tobacco plants and showed that the virus was anautocatalytic protein that could multiply in living cells(Nobel Prize)
Bawden in 1936: virus was protein and RNA 1939 virus particles first seen with the electron microscope 1956 Gierer and Schramm: protein could be removed from
the virus and that the nucleic acid carried the genetic info
1962 Agar double diffusion serological test for viruses 1977 ELISA test for viruses 1975 monoclonal antibodies Nucleic acid studies: 1936 to 1977 1971 Diener determined that potato spindle tuber disease
was caused by a viroid (What is a viroid? Is it unique toplants?)
1980 Cauliflower mosaic virus, a circular double strandedDNA, was first sequenced (8,000 base pairs)
1982 complete sequence of single strandedTMV was done
Protozoa and Phytoplasmas 1909 Lafont observed flagellate protozoa in latex bearing
cells of laticiferous plants of Euphorbiaceae 1931: Stahel found flagellates infecting phloem of coffee 1963 Vermeulen showed evidence of flagellates in coffee and
in 1976 they were associated with coconut and oil palms 1967 Doi in Japan observed mycoplasmalike bodies in the
phloem of plants infected with leafhopper diseases 1967 Ishiie and showed that symptoms disappeared
temporarily if treated with tetracycline antibiotics 1971 Davis et al observed spiroplasmas in corn stunt disease
Diagnosis of Plant Diseases Pathogen or Environment: Look for sign of the pathogen or
symptom ( already described) which might infer pathogen Infectious diseases: diseases caused by pathogens Characterized by their presence on or inside the plants Parasitic higher plants: dodder, mistletoe, witchweed or
broomrape growing on the surface Nematodes: presence on or in a plant or in its rhizosphere,
but must be distinguished from nonparasitic ones;taxonomic
Fungi: must determine if it is a parasite or saprophyte;morphology of its structures; taxonomy and reports ofpathogenicity on plant species
Bacteria: diagnosis of the disease and the ID of the bacteriumin based primarily on symptoms, constant presence of thebacterium and absence of other pathogens; culturing,selective media and taxonomic characterization; serology,PCR, fatty acid analysis; avoidance of looking at secondaryorganisms; fastidious bacteria difficult (small and sometimesin low numbers) and are difficult to culture.
Phytoplasmas and spiroplasmas: usually cause stunting andyellowing of plants and other specific symptoms; electronmicroscopy and PCR is used; P’s not culturable, S’s are;often graft transmissible and sensitive to antibiotics, insectvectored
Viruses and viroids: distinctive symptoms on hosts and oftenidentified this way; virus transmission tests to specific hostsby sap transmission or grafting; transmission by certaininsects; serological test available for many, PCR, electronmicroscopy, light microscopy of viral inclusions;electrophoretic and PCR tests for viroids;
Noninfectious diseases: lack or over abundance ofsubstances, environmental factors (look for specificsymptoms)
Koch’s Rules (Postulates) Pathogen found associated with the disease in all diseased
plants Pathogen must be isolated and grown in pure culture on
nutrient media and its characteristics described or on asusceptible host plant (obligate parasites) and it appearanceand effects recorded
Pathogen from pure culture must be inoculated to healthyplants and must produce the same disease symptoms
The pathogen must be reisolated from the disease plant andit characteristics must be the same as in step 2.
What about viruses and viroids? What about a positive PCR and accompanying sequence
determination? Is this enough?
Break
Parasitism and Disease Development Infectious diseases: result from infection of a plant by a
pathogen Parasite: an organism that lives on or in some other
organism and obtains its food from the later Parasitism: relationship between a parasite and its host Is a plant parasite a pathogen? Terms to consider: symbiosis and pathogenicity
Symbiosis and Pathogenicity Symbiosis: mutual benefit to parasite and host Pathogenicity: the ability of the parasite to interfere with one
or more essential functions of the plant with parasitismplaying an important but not always the most importantrole.
Considerations Obligate parasites Nonobligate parasites Facultative saprophytes Facultative parasites In N. America: 8000 species of fungi cause 80,000 diseases,
200 species of bacteria and 75 phytoplasmas, more than 500viruses attack crops.
Development of Disease What is necessary for a plant disease to occur? Plant Pathogen Susceptible plant Environment for disease to develop or express itself Called the “DiseaseTriangle” Variability of each component can change the degree of
disease severity Could there be an additional component of the triangle?
Stages in Development of Disease:
The Disease Cycle Infectious diseases have more or less distinct events in the
succession that lead to the development and perpetuation ofthe disease and the pathogen
This chain of events is the disease cycle May correspond to the pathogen life cycle but not always Disease cycle involves changes in the plant and the
symptoms of the plant as well as in the pathogen and spanstime within the growing season and from one season to thenext
The Disease Cycle Inoculation Penetration Establishment of infection Colonization and growth Reproduction of the pathogen (could be further growth) Dissemination of the pathogen Survival of the pathogen in absence of the host(overwintering or oversummering)
Inoculation Pathogen contact with the host- inoculum Spores, sclerotia, fragments of mycelium, individuals ofbacteria, phytoplasmas, viruses, viroids, etc. Propagules: single units of inoculum of a pathogen Types of inoculum: primary, secondary Sources of inoculum: debris, soil, tubers, transplants, seed Landing or arrival of inoculum: wind, water, insects
Prepenetration Phenomena Germination of spores or seeds Hatching of nematode eggs Attachment of pathogen to the host plant Recognition between host and pathogen
Penetration Direct penetration through intact plant surfaces Through wounds Natural openings
Infection Process by which pathogens establish contact with susceptible cells or tissues and procure nutrition from them. Successful infections result in symptoms in or on the host Latent infections do not produce symptoms immediately Incubation period is the time between inoculation andappearance of disease symptoms Invasion: movement throughout the host
Colonization Growth and reproduction of the pathogen
Dissemination Further growth sometimes outside the host to new tissues ornew plants By Air By Water By insects, mites, nematodes and other vectors Humans
Overwintering or Oversummering Survival in plant tissues or plant propagative parts In infected plant debris Soil Seeds (parasitic higher plants)
Types of Disease Cycles Monocyclic: single cycle pathogens such as smuts, Polycyclic: more than one generation per growth season;multi cycle pathogens such as downy mildews, late blight ofpotato Polycyclic diseases usually cause explosive epidemics sincethey complete many disease cycles per year and the amountof inoculum is multipled many fold Poletic pathogens: multi-year pathogens; survive in perennialhosts and have as much inoculum at the beginning of theseason as the ended with in the last season. Examples areviruses and phytoplasmas and fungal vascular wilts
Terminology List of terms to study that will be helpful in the cominglectures
plant pathology healthy plant Plant disease symptoms Macroscopic symptoms microscopic symptoms vascular wilt systemic infection hyperplasia hypertrophy
Koch’s Rules Infectious disease symbiosis pathogenicity Obligate parasites Nonobligate parasites Facultative saprophytes Facultative parasites disease triangle disease cycle Monocyclic Polycyclic Poletic pathogens
• Citrus tristeza • Citrus stubborn• Citrus Greening• citrus variegated chlorosis
Introduction and historical background of vector-borne plant disease
Historical background of vector-borne plant diseases
Sources:
• Insect Transmission of Plant Diseases (Chapter 1)
Julian Gilbert Leach 1940• Insects in Relation to Plant Disease (Chapter 1)
Walter Carter 1962
The first step: evidence that insects can transmit pathogens• Waite (1891) first established the fact that insects can
transmit plant pathogens (fire blight of pears is transmitted by bees).
• At almost same time, it was discovered that insects and other arthropods were great importance as vectors for human and animal diseases.
Challenges of studying insect-disease complex
• Although discovering the role of insects in transmitting diseases was a major step, progress in the study of insect transmission of plant diseases was slow.
• A lack of communication and cooperation between plant pathologists and entomologists delayed for many years the discovery of important facts about insect-plant disease relationships.
The need for a cooperative effort • Discovery of viral plant diseases and the role of insect in
the transmission of viruses has greatly stimulated the interest of both plant pathologists and entomologists on one main topic “the insect transmission of plant diseases”.
• The necessity for cooperation between entomologists and plant pathologists to devise the solution for these problems is now recognized by both groups.
The most destructive plant diseases are vector-borne
The most economica l l y
impor tant insec t s se r ve as vec tor s
Plant Pathologist
Entomologist
The economic threshold (ET) is zero
Vectors transmit pathogens from plant to plant in short and long distance
Control the vector, key to control the disease
Disturb the bacterial growth in plant, key to control the disease
Disturbing the pathogen acquisition by the vector from plant can lead to management of the disease
Economic Injury Level (EIL) - the lowest pest population level that will cause economic damage or the critical population density where the loss caused by the pest equals in monetary value to the cost of management.
Economic Threshold or Action Threshold (ET) - the point at which management actions should be taken to prevent an increasing pest population from exceeding the economic injury level.
The ET always represents a pest density or level of pest damage lower than the EIL.
In special cases, where pests serve as vectors of plant or animal diseases, the economic threshold (ET) is zero. A single pest attack may cause the death of a plant or animal.
Factors affect plant disease epidemics
Factors affect plant disease epidemics
Pierce’s disease in grapeXylella fastidiosa
Vector-borne diseases can kill millions of plants/trees fast!
Photo A.H. Purcell 2002
GWSS as a vector in Temecula Valley3 years after first symptomatic plant
Pierce’s disease in grape
Any questions?