1.Surveying Macrofungi of Yosemite& Pt Reyes Mochella sp nova Conocybe sp nova Tuber spMycena puraAgrocybe pediades

2. What is a macrofungus? Answer: a fungus that produces amacroscopically fruiting structure that can beseen (and sometimes identified) without amicroscope. 3. Macrofungi can be very macro Boletus edulis Kabir Peay 4. Or they can be just a few millimetersDasyscyphus nudipesvar. minor 5. Who is involved? Members of the amateur mushroom groups:Bay Area Mycological Society, MycologicalSociety of San Francisco, Sonoma MycologicalAssociation, Santa Cruz Fungus Federation Members of the general public Professional mycologist, especially studentsand postdocs, from Berkeley, Davis, SF State,Humboldt State 6. David Arora, Norm Andresen, Fred Stevens, Mike Wood,Erin Blanchard , ChristianSchwarz, Debbie Viess, David Rust, and many others not pictured 7. Some of the Amateurs are amazingCortinarius (>1000 described species)Dimitar Bojantchev 8. Galerina an example of an LBM (little brownmushroom) genus Doug Smith 9. Mike Wood the author of Mykoweb 10. The Berkeley crew: Jennifer Kerekes, Anthony Amend, Kabir Peay,Nicole Hynson, Sir David Read, Nhu Nguyen, Todd Osmundsen Else Vellinga 11. Why do we want to survey the macrofungi of the National Parks? Surprising little is known about the mycofloraof North America The park contains some of the best and mosteasily accessible examples of native plantcommunities Fungi are crucial components of all terrestrialecosystems Provides ground truth for ITS sequence data! 12. Example of the Pt Reyes Survey http://www.mykoweb.com/PtReyes/ The survey was structured into five publicmycoblitzs People from the MSSF, FFSC, BAMS, and SOMAprovided most of the manpower for collecting andidentifying. Collections were sequenced (ITS and sometimesRPB2) 13. We assigned people routes and zones to cover 14. Collection instructions were passed out: 15. Identification of the collections Collections were made and coarsely sorted on the Saturday ofeach foray, and then examined more closely, photographedand dried on the following Sunday. Collection tags from common species were saved andcatalogued to provide distribution data Species that were new to the park list, or for which nopermanent collections were previous made were saved. Sunday a smaller group at Berkeley sorted through the thelatter collections, examined some with microscopes, selectedthose worth saving, and photographed and dried them. 16. Curation of collections Collection data were entered in a database Collections were accessioned into the UCHerbarium Data and images were be made webaccessible Eventual goal is to sequence the ITS regionfrom all of them, and RPB1 from all collections 17. Inocybe centralPeter Kennedy Kabir Peay 18. DNA sequences helped to sort out the difficult groups 19. Increase in number of recorded specieswith each mycoblitz at Pt. Reyes600500400300200100001 23 45 6 7 20. Inverse Ranked Abundance Curve of Pt. Reyes fungal species by number of collections 21. A different plan for Yosemite Fewer people (but many specialists) No mycoblitzes, but lots of small groupcollecting Voucher specimens with similar labeling andphotographic conventions as at Pt. Reyes DNA sequences still a must Better internet coordination 22. The Mushroom Observer Nathan Wilsons cool tool Google Mushroom Observer Plug in Yosemite in the find widow 23. In 8 month we when from 39 pages to 63 pages of15 collections/page 24. Google Doc: the non-public coordination interface 25. A small piece of the collectionsspreadsheet on Google Docs 26. Some numbers from the first years survey >500 collections were made, 110 have beensequenced in one direction for the ITS region The collections included >130 species, and 111genera 90% of the species and 62% of the genera were newrecords for the Yosemite This more than doubled the number of speciesknown from the park and increases the knowngenera by 88% When compared to Pt Reyes 62% of the species and47% of genera were unique to the park Several new species have been described 27. Time consuming issues Keeping up with curation of specimens Keeping up with the website Keeping up with sequence acquisition anddissemination Dealing with permits (especially fromYosemite!) 28. My Dream: Expand this effort to survey the continentwith a goal of producing an North Americanmycoflora Use this is a platform for training bothprofessions (grad students and postdocs), andthe general public Find major funding for this